Data/hora: 18/06/2024 13:41:00

Biblioteca(s): 	Embrapa Milho e Sorgo.	
Data corrente: 	14/04/2009	
Data da última atualização: 	30/05/2018	
Autoria: 	COELHO, M. R. R.; CARNEIRO, N. P.; MARRIEL, I. E.; SELDIN, L.	
Afiliação: 	Marcia Reed Rodrigues Coelho, UFRJ; NEWTON PORTILHO CARNEIRO, CNPMS; IVANILDO EVODIO MARRIEL, CNPMS; Lucy Seldin, UFRJ.	
Título: 	Molecular detection of nifH gene-containing Paenibacillus in the rhizosphere of sorghum (Sorghum bicolor) sown in Cerrado soil.	
Ano de publicação: 	2009	
Fonte/Imprenta: 	Letters in Applied Microbiology, Oxford, v. 48, n. 5, p. 611-617, 2009.	
DOI: 	10.1111/j1472.765X.2009.02578.x	
Idioma: 	Inglês	
Conteúdo: 	Aims: To develop a polymerase chain reaction (PCR)-based approach for the detection of nifH gene-containing Paenibacillus in environmental samples. Methods and Results: The primers, nitHPAENf and nitHPAENr, were designed and tested with DNA from: (i) strains of different nitrogen-fixing Paenibacillus species, (ii) strains of other nitrogen-fixing genera and (iii) rhizosphere of sorghum sown in Cerrado soi! amended with either 12 or 120 kg ha-1 of nitrogen fertilizer. All nitrogen-fixing Paenibacillus strains tested and the DNA samples from rhizosphere soil were amplified when these primers were used, generating a 280 bp fragment. When the PCR products obtained from both sorghum rhizospheres were cloned and sequenced, the majority of the clones analysed could be identified as Paenibacillus durus. Moreover, a greater diversity in the nifH sequences could be observed in the rhizosphere treated with a high amount of nitrogen fertilizer. Conclusions: Nitrogen fertilization slightly influenced the structure of the nifH gene-containing Paenibacillus community in sorghum rhizospheres cultivated in Cerrado soil. Significance and Impact of the Study: The PCR detection method developed is adequate to assess the presence of nifH gene-containing Paenibacillus in the environment and can be used in future to determine the ecological role of this group of micro-organisms for the nitrogen input to the plants.	
Palavras-Chave: 	nifH; PCR; Primers; Sorghum.	
Thesaurus Nal: 	Paenibacillus.	
Categoria do assunto: 	S Ciências Biológicas	
Registro original: 	Embrapa Milho e Sorgo (CNPMS)	
Exemplares: 	CNPMS - (21604-1) - UPC - AP - DD -  -  -  - DIS