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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
18/12/2023 |
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Data da última atualização: |
18/12/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, C. G. N. da; MONTEIRO, E. de C.; DINIZ, P. P.; TERRA, L. A.; SCHWAB, S.; REIS, V. M.; ARAUJO, J. L. S. de; URQUIAGA, S. |
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Afiliação: |
CLEUDISON GABRIEL NASCIMENTO DA SILVA, UFRRJ; EDEVALDO DE CASTRO MONTEIRO, UFRRJ; PRISCILA PEREIRA DINIZ, UFRRJ; LEONARDO ARAUJO TERRA, UFRRJ; STEFAN SCHWAB, CNPAB; VERONICA MASSENA REIS, CNPAB; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; SEGUNDO SACRAMENTO U CABALLERO, CNPAB. |
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Título: |
Designing and validation of specifc primers for the quantitative detection of bacteria in sugarcane inoculant. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 54, p. 2627–2640, 2023. |
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ISSN: |
1678-4405 |
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Idioma: |
Inglês |
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Conteúdo: |
Endophytic diazotrophic plant growth-promoting bacteria Herbaspirillum rubrisubalbicans (HCC103), Herbaspirillum seropedicae (HRC54), Paraburkholderia tropica (Ppe8T), Gluconacetobacter diazotrophicus (Pal5T), and Nitrospirillum amazonense (CBAmC) have been used as inoculants for sugarcane. The genome sequences of these strains were used to design a set of specifc primers for the real-time PCR (qPCR) assay. Primer specifcity was confrmed by conventional PCR using the genomic DNAs of 25 related bacterial species and the fve target strains. The qPCR assays were conducted using root and shoot samples from two sugarcane varieties (RB867515 and RB92579). These samples were collected both with and without inoculation, using the target strains specifed in this study. The sugarcane plants were grown in a greenhouse, utilizing a substrate composed of sterile sand and vermiculite in a 2:1 ratio, for a duration of 55 days. The primers designed for this study successfully amplifed target DNA fragments from each of the bacterial species, enabling their diferentiation at the species level. The total bacterial population present in the sugarcane quantifed using qPCR was on average 105.2 cells g−1 of fresh tissue. Across both evaluated varieties, it was observed that the population of inoculated bacteria tended to decrease over time and became more concentrated in the sugarcane roots compared to the aerial parts. The qPCR results suggest that both the host and the microbes infuence the endophytic population and the bacterial number decreases with plant age. MenosEndophytic diazotrophic plant growth-promoting bacteria Herbaspirillum rubrisubalbicans (HCC103), Herbaspirillum seropedicae (HRC54), Paraburkholderia tropica (Ppe8T), Gluconacetobacter diazotrophicus (Pal5T), and Nitrospirillum amazonense (CBAmC) have been used as inoculants for sugarcane. The genome sequences of these strains were used to design a set of specifc primers for the real-time PCR (qPCR) assay. Primer specifcity was confrmed by conventional PCR using the genomic DNAs of 25 related bacterial species and the fve target strains. The qPCR assays were conducted using root and shoot samples from two sugarcane varieties (RB867515 and RB92579). These samples were collected both with and without inoculation, using the target strains specifed in this study. The sugarcane plants were grown in a greenhouse, utilizing a substrate composed of sterile sand and vermiculite in a 2:1 ratio, for a duration of 55 days. The primers designed for this study successfully amplifed target DNA fragments from each of the bacterial species, enabling their diferentiation at the species level. The total bacterial population present in the sugarcane quantifed using qPCR was on average 105.2 cells g−1 of fresh tissue. Across both evaluated varieties, it was observed that the population of inoculated bacteria tended to decrease over time and became more concentrated in the sugarcane roots compared to the aerial parts. The qPCR results suggest that both the host and the microbes infuence the endo... Mostrar Tudo |
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Palavras-Chave: |
Diazotrophic bacteria; DPGPB quantifcation; Primers; Real Time PCR. |
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Thesagro: |
Saccharum Officinarum. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02399naa a2200277 a 4500
001 2159851
005 2023-12-18
008 2023 bl uuuu u00u1 u #d
022 $a1678-4405
100 1 $aSILVA, C. G. N. da
245 $aDesigning and validation of specifc primers for the quantitative detection of bacteria in sugarcane inoculant.$h[electronic resource]
260 $c2023
520 $aEndophytic diazotrophic plant growth-promoting bacteria Herbaspirillum rubrisubalbicans (HCC103), Herbaspirillum seropedicae (HRC54), Paraburkholderia tropica (Ppe8T), Gluconacetobacter diazotrophicus (Pal5T), and Nitrospirillum amazonense (CBAmC) have been used as inoculants for sugarcane. The genome sequences of these strains were used to design a set of specifc primers for the real-time PCR (qPCR) assay. Primer specifcity was confrmed by conventional PCR using the genomic DNAs of 25 related bacterial species and the fve target strains. The qPCR assays were conducted using root and shoot samples from two sugarcane varieties (RB867515 and RB92579). These samples were collected both with and without inoculation, using the target strains specifed in this study. The sugarcane plants were grown in a greenhouse, utilizing a substrate composed of sterile sand and vermiculite in a 2:1 ratio, for a duration of 55 days. The primers designed for this study successfully amplifed target DNA fragments from each of the bacterial species, enabling their diferentiation at the species level. The total bacterial population present in the sugarcane quantifed using qPCR was on average 105.2 cells g−1 of fresh tissue. Across both evaluated varieties, it was observed that the population of inoculated bacteria tended to decrease over time and became more concentrated in the sugarcane roots compared to the aerial parts. The qPCR results suggest that both the host and the microbes infuence the endophytic population and the bacterial number decreases with plant age.
650 $aSaccharum Officinarum
653 $aDiazotrophic bacteria
653 $aDPGPB quantifcation
653 $aPrimers
653 $aReal Time PCR
700 1 $aMONTEIRO, E. de C.
700 1 $aDINIZ, P. P.
700 1 $aTERRA, L. A.
700 1 $aSCHWAB, S.
700 1 $aREIS, V. M.
700 1 $aARAUJO, J. L. S. de
700 1 $aURQUIAGA, S.
773 $tBrazilian Journal of Microbiology$gv. 54, p. 2627–2640, 2023.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Biblioteca |
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| Registros recuperados : 9 | |
| 2. |  | MICHEL, D. C.; PASSOS, S. R.; ARAUJO, J. L. S. de; BARAÚNA, A. C.; SILVA, K. da; LEME, M. M. P.; MELO, I. S. de; DE MEYER, S. E.; O’HARAM G.; ZILLI, J. E. Bradyrhizobium centrolobii and Bradyrhizobium macuxiense sp. nov. isolated from Centrolobium paraense grown in soil of Amazonia, Brazil Archives of Microbiology, v. 199, n. 5, p. 657-664, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrobiologia; Embrapa Roraima. |
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| 3. | | MICHEL, D. C.; PASSOS, S. R.; ARAUJO, J. L. S. de; BARAÚNA, A. C.; SILVA, K. da; LEME, M. M. P.; MELO, I. S. de; DE MEYER, S. E.; O'HARAM, G.; ZILLI, J. E. Bradyrhizobium centrolobii and Bradyrhizobium macuxiense sp. nov. isolated from Centrolobium paraense grown in soil of Amazonia, Brazil Archives of Microbiology, v. 199, n. 5, p. 675-664, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 4. | | SILVA, K. da; DE MEYER, S. E.; ROUWS, L. F. M.; FARIAS, E. N. C.; SANTOS, M. A. O. dos; O'HARA, G.; ARDLEY, J. K.; WILLMES, A.; PITARD, R. M.; ZILLI, J. E. Bradyrhizobium ingae sp. nov., isolated from effective nodules of Inga laurina grown in Cerrado soil. International journal of Systematic and Evolutionary Microbiology, Online, 10 jul. 2014| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Roraima. |
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| 5. | | SILVA, K. da; DE MEYER, S. E.; ROUWS, L. F. M.; FARIAS, E. N. C.; SANTOS, M. A. O. dos; O'HARA, G.; ARDLEY, J. K.; WILLMES, A.; PITARD, R. M.; ZILLI, J. E. Bradyrhizobium ingae sp. nov., isolated from effective nodules of Inga laurina grown in Cerrado soil. International journal of Systematic and Evolutionary Microbiology, Online, 10 jul. 2014| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 6. | | SILVA, F. V.; de MEYER, S. E.; ARAUJO, J. L. S. de; BARBÉ, T. da C.; XAVIER, G. R.; O'HARA, J. K. A.; RUMJANEK, N. G.; WILLEMS, A.; ZILLI, J. E. Bradyrhizobium manausense sp. nov., isolated from effective nodules of Vigna unguiculata grown in Brazilian Amazonian rainforest soils. International Journal of Systematic and Evolutionary Microbiology, v. 64, p. 2358-2363, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 7. | | ZILLI, J. E.; BARAÚNA, A. C.; SILVA, K. da; MEYER, S. E.; FARIAS, E. do N. C.; KAMINSKI, P.; COSTA, I. B. da; ARDLEY, J. K.; WILLEMS, A.; CAMACHO, N. N.; DOURADO, F. dos S.; O'HARA, G. Bradyrhizobium neotropicale sp. nov. isolated from effective nodules of Centrolobium paraense grown in soil of Amazonia, Brazil. International journal of Systematic and Evolutionary Microbiology, v. 64, pt 12, p. 3950-3957, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrobiologia; Embrapa Roraima. |
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| 8. | | JAMES, E. K.; FARIA, S. M. de; HUGHE, C. E.; VENTER, S. N.; AMETSITSI, G. K. D.; ARDLEY, J.; BEUKES, C. W.; CARDOSO, D.; CHAN, A.; CHIMPHANG, S.; REIS JUNIOR, F. B. dos; GEHLO, H. S.; ROSS, E.; KOENEN, E. J. M.; LIMA, H. de C.; MALUK, M.; MAVIMA, L.; DE MEYER, S. E.; MUASYA, M.; PRIN, Y.; RINGELBERG, J. J.; SIMON, M. F.; SPRENT, J. I.; STEENKAMP, E.; TAK, N.; YOUNG, P. W.; ZARTMANN, C. What can the diversity of nodulation phenotypes and rhizobial symbionts in the caesalpinioideae tell us about the evolution of the legume-rhizobium symbiosis? In: INTERNATIONAL LEGUME CONFERENCE, 8., 2023, Pirenópolis. Integrating knowledge on the legume family: [abstracts]. Pirenópolis: [s.n.], 2023.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
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| 9. | | JAMES, E. K.; FARIA, S. M. de; HUGHE, C. E.; VENTER, S. N.; AMETSITSI, G. K. D.; ARDLEY, J.; BEUKES, C. W.; CARDOSO, D.; CHAN, A.; CHIMPHANG, S.; REIS JUNIOR, F. B. dos; GEHLO, H. S.; ROSS, E.; KOENEN, E. J. M.; LIMA, H. de C.; MALUK, M.; MAVIMA, L.; DE MEYER, S. E.; MUASYA, M.; PRIN, Y.; RINGELBERG, J. J.; SIMON, M. F.; SPRENT, J. I.; STEENKAMP, E.; TAK, N.; YOUNG, P. W.; ZARTMANN, C. What can the diversity of nodulation phenotypes and rhizobial symbionts in the caesalpinioideae tell us about the evolution of the legume-rhizobium symbiosis? In: INTERNATIONAL LEGUME CONFERENCE, 8., 2023, Pirenópolis. Integrating knowledge on the legume family: [abstracts]. Pirenópolis: [s.n.], 2023.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia; Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 9 | |
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