Data/hora: 13/06/2024 01:42:05

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 01/04/2005
Data da última atualização: | 01/04/2005
Autoria: | TERZANO, G. M.; ROMANO, D.; SENATORE, E. M.; DE MAURO, G.; SCATÀ, M. C.; BORGHESE, A.
Título: | Recovery of oocyties by laparoscopic follicle aspiration in FSH-treated goats.
Ano de publicação: | 2000
Fonte/Imprenta: | Theriogenology, v. 53, n. 1, p. 509 2000.
Idioma: | Inglês
Notas: | Edição de proceedings Annual Conference of the International Embryo Transfer Society, Maastricht, The Netherlands, jan. 2000.
Conteúdo: | The objective oft his preliminary study was to compare the number and quality of oocytes recovered by laparoscopic follicle aspiration from donor goats subjected to two different schedules of superovulatory treatments with a porcine gonadotropin pituitary extract (Pluset, Serono Veterinary, Italy). Sixteen multiparous Jonica goats were used in June. Estrous was synchronized by insertion of 45 mg fluorogestone acetate-impregnated intravaginal sponges (Chronogest, Intervet, Italy) for 10 d and by the injection of 3 mg of PGF2a (Prosolvin, Intervet, Italy) administered 8 d after sponge insertion. The goats were randomIy assigned to two different treatment groups (group A and B, each n=8). Group A: 250 lU pFSH, injected (sc) as a single injection 24 h before sponge removal; group B: 250 IU pFSH injected (im) 12 h apart in a decreasing dosage over 4 d (65,65,30,30,20,20, 10, 10 lU), starting 72 h before sponge removal. Prior to laparoscopy the goats were deprived of food and water for 24 and 12 h, respectively. Aspirations were performed 24 h following sponge removal; donors were sedated with (im) 0.05 mg/kg body weight of atropin sulphate (Angelini, Italy) and 0.2 mg/kg body weight of xilazine (Rompun, Bayer, Germany) followed by (iv) 4 mg/kg body weight ofketamine (Inoketam 1000, Virbac, France). A 7 mm laparoscope, a 5 fim atraumatic grasping forceps and an 18 gauge aspiration needle connected to a vacuum line (10 mL/min) were used for ovarian manipulation and follicle aspiration. Aspiration medium consisted of phosphate-buffered saline (Euroclone, Ltd, UK) supplemented with 10 IU/mL heparin, 1% antibiotic-antimycotic and 1 g/L polyvinyl alcohol (Sigma, USA). Number of observed follicles, aspirated follicles, recovered Oocytes and viable Oocytes were recorded. Data were anaIyzed by least squares anaIysis of variance using the GLM procedure (SAS/STAT User's Guide). One goat was eliminated from data anaIysis due to ovarian adhesions and difficulties in manipulation. The results are reported as mean + SEM as follows. The results suggest that a simpler superovulatory protocol can be used in goats without affecting follicle recruitment and oocyte recovery. Further research is in progress to study goat oocytes quality and maturation in vitro.
Palavras-Chave: | Ednocrinologia; FSH; Ovocito.
Thesagro: | Caprino; Hormônio; Laparoscopia; Reprodução Animal.
Categoria do assunto: | --
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (17593-1) - ADC - AP - -- -  -  - v.53,p.509 - DIS