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| 3. |  | ROUT, P. K.; SAXENA, V. K.; KHAN, B. U.; MANDAL, A.; SINGH, L. B.; ROY, R. Impact of the improvement and conservation programme of Jamunapari goats in their home tract; an Indian experience. In: INTERNATIONAL CONFERENCE ON GOATS, 7., 2000, Tours, Poitiers, France. Proceedings... Paris: INRA: IGA: Institut de Elevage, 2000. v. 2, p. 936-938.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 4. | | SHARMA, D. K.; CHAUHAN, P. P.; SAXENA, V. K.; AGRAWAL, R. D. Experimental trypanosomiasis in Barbari goats: a study on leukocyte response. In: INTERNATIONAL CONFERENCE ON GOATS, 7., 2000, Tours, Poitiers, France. Proceedings... Paris: INRA: IGA: Institut de Elevage, 2000. v. 2, p. 857-859.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
07/11/2011 |
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Data da última atualização: |
12/12/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
LITHOLDO JÚNIOR, C. G.; GOMES, E. V.; LOBO JUNIOR, M.; NASSER, L. C. B.; PETROFEZA, S. |
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Afiliação: |
C. G. LITHOLDO JÚNIOR, UFG; E. V. GOMES, UFG; MURILLO LOBO JUNIOR, CNPAF; LUIZ CARLOS BHERING NASSER; SILVANA PETROFEZA. |
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Título: |
Genetic diversity and mycelial compatibility groups of the plant-pathogenic fungus Sclerotinia sclerotiorum in Brazil. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 10, n. 2, p. 868-877, 2011. |
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Idioma: |
Inglês |
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Conteúdo: |
The genetic variability of 40 Sclerotinia sclerotiorum isolates from various fields widely distributed throughout Brazil and different host crops was analyzed using RAPD markers and mycelial compatibility groupings (MCGs). The isolates were characterized using 16 random primers of the OPERON series, which produced 121 DNA fragments. UPGMA cluster analysis using Jaccard?s genetic distance and MCGs allowed separation of the isolates into three clusters, with similarity indices of 68.2, 61.8, and 61.8%, and five MCGs. The haplotypes obtained with RAPD markers provided very characteristic groupings of S. sclerotiorum isolates according to MCG, but did not show any relationship with geographic origin or host type. Furthermore, analysis of molecular variance demonstrated that 99.1% of the observed variation was a result of genetic differences between individuals; the host culture did not have a significant effect. This is the first report of high level variability of S. sclerotiorum in Brazil based on the study of isolates of wide geographical origin, supported by RAPD markers and MCGs. These results endorse the prevalence of sexual reproduction in tropical and subtropical regions in contrast to clonal reproduction in temperate regions. |
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Palavras-Chave: |
Diversidade genética. |
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Thesagro: |
Mofo branco; Sclerotinia sclerotiorum. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/45576/1/gmr9371.pdf
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Marc: |
LEADER 01919naa a2200205 a 4500
001 1905022
005 2011-12-12
008 2011 bl uuuu u00u1 u #d
100 1 $aLITHOLDO JÚNIOR, C. G.
245 $aGenetic diversity and mycelial compatibility groups of the plant-pathogenic fungus Sclerotinia sclerotiorum in Brazil.$h[electronic resource]
260 $c2011
520 $aThe genetic variability of 40 Sclerotinia sclerotiorum isolates from various fields widely distributed throughout Brazil and different host crops was analyzed using RAPD markers and mycelial compatibility groupings (MCGs). The isolates were characterized using 16 random primers of the OPERON series, which produced 121 DNA fragments. UPGMA cluster analysis using Jaccard?s genetic distance and MCGs allowed separation of the isolates into three clusters, with similarity indices of 68.2, 61.8, and 61.8%, and five MCGs. The haplotypes obtained with RAPD markers provided very characteristic groupings of S. sclerotiorum isolates according to MCG, but did not show any relationship with geographic origin or host type. Furthermore, analysis of molecular variance demonstrated that 99.1% of the observed variation was a result of genetic differences between individuals; the host culture did not have a significant effect. This is the first report of high level variability of S. sclerotiorum in Brazil based on the study of isolates of wide geographical origin, supported by RAPD markers and MCGs. These results endorse the prevalence of sexual reproduction in tropical and subtropical regions in contrast to clonal reproduction in temperate regions.
650 $aMofo branco
650 $aSclerotinia sclerotiorum
653 $aDiversidade genética
700 1 $aGOMES, E. V.
700 1 $aLOBO JUNIOR, M.
700 1 $aNASSER, L. C. B.
700 1 $aPETROFEZA, S.
773 $tGenetics and Molecular Research$gv. 10, n. 2, p. 868-877, 2011.
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Embrapa Arroz e Feijão (CNPAF) |
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