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 | Acesso ao texto completo restrito à biblioteca da Embrapa Solos. Para informações adicionais entre em contato com cnps.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
11/01/2019 |
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Data da última atualização: |
11/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MATTOS, B. B.; MONTEBIANCO, C.; ROMANEL, E.; SILVA, T. da F.; BERNABÉ, R. B.; SIMAS-TOSIN, F.; SOUZA, L. M.; SASSAKI, G. L.; VASLIN, M. F. S.; BARRETO-BERGTER, E. |
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Afiliação: |
BIANCA BRAZ MATTOS, CNPS; CAROLINE MONTEBIANCO, UFRJ; ELISSON ROMANEL, UFRJ/USP; TATIANE DA FRANCA SILVA, UFRJ/USP; RENATO BARROSO BERNABÉ, UFRJ; FERNANDA SIMAS-TOSIN, UFPR; LAURO M. SOUZA, UFPR; GUILHERME L. SASSAKI, UFPR; MAITE F. S. VASLIN, UFRJ; ELIANA BARRETO-BERGTER, UFRJ. |
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Título: |
A peptidogalactomannan isolated from Cladosporium herbarum induces defense-related genes in BY-2 tobacco cells. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Plant Physiology and Biochemistry, v. 126, p. 206-216, May 2018. |
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DOI: |
https://doi.org/10.1016/j.plaphy.2018.02.023 |
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Idioma: |
Inglês |
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Conteúdo: |
Cladosporium herbarum is a plant pathogen associated with passion fruit scab and mild diseases in pea and soybean. In this study, a peptidogalactomannan (pGM) of C. herbarum mycelium was isolated and structurally characterized, and its role in plant-fungus interactions was evaluated. C. herbarum pGM is composed of carbohydrates (76%) and contains mannose, galactose and glucose as its main monosaccharides (molar ratio, 52:36:12). Methylation and C-13-nuclear magnetic resonance (C-13-NMR) spectroscopy analysis have shown the presence of a main chain containing (1 -> 6)-linked a-D-Manp residues, and beta-D-Galf residues are present as (1 -> 5)-interlinked side chains. beta-Galactofuranose containing similar structures were characterized by our group in A. fumigants, A. versicolor, A. flavus and C. resinae. Tobacco BY-2 cells were used as a model system to address the question of the role of C. herbarum pGM in cell viability and induction of the expression of plant defense-related genes. Native and partially acid hydrolyzed pGMs (lacking galactofuranosyl side-chain residues) were incubated with BY-2 cell suspensions at different concentrations. Cell viability drastically decreased after exposure to more than 400 mu g ml(-1) pGM; however no cell viability effect was observed after exposure to a partially acid hydrolyzed pGM. BY-2 cell contact with pGM strongly induce the expression of plant defense-related genes, such as phenylalanine ammonia lyase (PAL) and lipoxygenase (LOX), as well as the pathogen-related PR-l alpha, PR-2 and PR-3 genes, suggesting that pGM activates defense responses in tobacco cells. Interestingly, contact with partially hydrolyzed pGM also induced defense related gene expression at earlier times than native pGM. These results show that the side chains of the (1 -> 5) linked beta-D-galactofuranosyl units from pGM play an important role in the first line fungus-plant interactions mediating plant responses against C. herbarum. In addition, it was observed that pGM and/or C. herbarum conidia are able to induced HR when in contact with tobacco leaves and in vitro plantlets roots, producing necrotic lesions and peroxidase and NO burst, respectively. MenosCladosporium herbarum is a plant pathogen associated with passion fruit scab and mild diseases in pea and soybean. In this study, a peptidogalactomannan (pGM) of C. herbarum mycelium was isolated and structurally characterized, and its role in plant-fungus interactions was evaluated. C. herbarum pGM is composed of carbohydrates (76%) and contains mannose, galactose and glucose as its main monosaccharides (molar ratio, 52:36:12). Methylation and C-13-nuclear magnetic resonance (C-13-NMR) spectroscopy analysis have shown the presence of a main chain containing (1 -> 6)-linked a-D-Manp residues, and beta-D-Galf residues are present as (1 -> 5)-interlinked side chains. beta-Galactofuranose containing similar structures were characterized by our group in A. fumigants, A. versicolor, A. flavus and C. resinae. Tobacco BY-2 cells were used as a model system to address the question of the role of C. herbarum pGM in cell viability and induction of the expression of plant defense-related genes. Native and partially acid hydrolyzed pGMs (lacking galactofuranosyl side-chain residues) were incubated with BY-2 cell suspensions at different concentrations. Cell viability drastically decreased after exposure to more than 400 mu g ml(-1) pGM; however no cell viability effect was observed after exposure to a partially acid hydrolyzed pGM. BY-2 cell contact with pGM strongly induce the expression of plant defense-related genes, such as phenylalanine ammonia lyase (PAL) and lipoxygenase (LOX), a... Mostrar Tudo |
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Palavras-Chave: |
Peptidogalactomannan. |
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Thesagro: |
Defesa Vegetal; Glicoproteína. |
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Thesaurus Nal: |
Cladosporium herbarum. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 03116naa a2200289 a 4500
001 2103593
005 2021-11-11
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.plaphy.2018.02.023$2DOI
100 1 $aMATTOS, B. B.
245 $aA peptidogalactomannan isolated from Cladosporium herbarum induces defense-related genes in BY-2 tobacco cells.$h[electronic resource]
260 $c2018
520 $aCladosporium herbarum is a plant pathogen associated with passion fruit scab and mild diseases in pea and soybean. In this study, a peptidogalactomannan (pGM) of C. herbarum mycelium was isolated and structurally characterized, and its role in plant-fungus interactions was evaluated. C. herbarum pGM is composed of carbohydrates (76%) and contains mannose, galactose and glucose as its main monosaccharides (molar ratio, 52:36:12). Methylation and C-13-nuclear magnetic resonance (C-13-NMR) spectroscopy analysis have shown the presence of a main chain containing (1 -> 6)-linked a-D-Manp residues, and beta-D-Galf residues are present as (1 -> 5)-interlinked side chains. beta-Galactofuranose containing similar structures were characterized by our group in A. fumigants, A. versicolor, A. flavus and C. resinae. Tobacco BY-2 cells were used as a model system to address the question of the role of C. herbarum pGM in cell viability and induction of the expression of plant defense-related genes. Native and partially acid hydrolyzed pGMs (lacking galactofuranosyl side-chain residues) were incubated with BY-2 cell suspensions at different concentrations. Cell viability drastically decreased after exposure to more than 400 mu g ml(-1) pGM; however no cell viability effect was observed after exposure to a partially acid hydrolyzed pGM. BY-2 cell contact with pGM strongly induce the expression of plant defense-related genes, such as phenylalanine ammonia lyase (PAL) and lipoxygenase (LOX), as well as the pathogen-related PR-l alpha, PR-2 and PR-3 genes, suggesting that pGM activates defense responses in tobacco cells. Interestingly, contact with partially hydrolyzed pGM also induced defense related gene expression at earlier times than native pGM. These results show that the side chains of the (1 -> 5) linked beta-D-galactofuranosyl units from pGM play an important role in the first line fungus-plant interactions mediating plant responses against C. herbarum. In addition, it was observed that pGM and/or C. herbarum conidia are able to induced HR when in contact with tobacco leaves and in vitro plantlets roots, producing necrotic lesions and peroxidase and NO burst, respectively.
650 $aCladosporium herbarum
650 $aDefesa Vegetal
650 $aGlicoproteína
653 $aPeptidogalactomannan
700 1 $aMONTEBIANCO, C.
700 1 $aROMANEL, E.
700 1 $aSILVA, T. da F.
700 1 $aBERNABÉ, R. B.
700 1 $aSIMAS-TOSIN, F.
700 1 $aSOUZA, L. M.
700 1 $aSASSAKI, G. L.
700 1 $aVASLIN, M. F. S.
700 1 $aBARRETO-BERGTER, E.
773 $tPlant Physiology and Biochemistry$gv. 126, p. 206-216, May 2018.
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| Registros recuperados : 12 | |
| 1. |  | SOUZA, M. G. de; ALMEIDA, O. C. de; SOUZA, A. das G. C. de. Avaliação de metodologia para inoculação de Moniliophthora perniciosa em cupuaçuzeiro. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 44., 2011, Bento Gonçalves. Anais... Tropical Plant Pathology, Brasília, DF, v. 36, p. 0558, 2011. 1 CD-ROM. Suplemento.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 9. | | NASCIMENTO, H. J. da S.; SOUZA, M. G. de; OLIVEIRA, M. de; ALMEIDA, O. C. de; SOUZA, A. das G. C. de; CHAVES, F. C. M. Avaliação do óleo essencial Piper hispidum com potencial antifúngico. In: REUNIÃO ANUAL DA SOCIEDADE INTERAMERICANA DE HORTICULTURA TROPICAL, 61., 2015, Manaus. Anais... Brasília, DF: Embrapa, 2015. p. 78.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 10. | | SILVA, M. S. da; SOUZA, M. G. de; ALMEIDA, O. C. de; SOUZA, A. C.; CHAVES, F. C.; OLIVEIRA, M. R. Efeito do óleo essencial de Piperaceae sobre Moniliophthora perniciosa (Sthael) Aime Phillips-Mora. do cupuaçuzeiro. In: SIMPÓSIO INTERNACIONAL DE BOTÂNICA APLICADA, 2.; SIMPÓSIO NACIONAL DE FRUTÍFERAS E ORNAMENTAIS DO NORTE E NORDESTE, 2., 2013, Manaus. Anais... Manaus: Ufam, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 11. | | BARACHO, M. B.; SOUZA, M. G. de; OLIVEIRA, M. R. de; ALMEIDA, O. C. de; SOUZA, A. das G. C. de; CHAVES, F. C. M. Efeito do óleo essencial de Piper tuberculatum sobre Moniliophthora perniciosa causador da vassoura-de-bruxa no cupuaçuzeiro. In: REUNIÃO ANUAL DA SOCIEDADE INTERAMERICANA DE HORTICULTURA TROPICAL, 61., 2015, Manaus. Anais... Brasília, DF: Embrapa, 2015. p. 89.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 12. | | SOUZA, M. G. de; SOUZA, A. das G. C. de; SOUSA, N. R. de; SILVA, G. F. da; ALMEIDA, O. C. de; BICHARA, K. P. de A.; REBELLO, R. P.; CRUZ, A. S. da. Seleção de variáveis na avaliação de progênies do cupuaçuzeiro (Theobroma grandiflorum) quanto à resistência a Moniliophthora perniciosa. In: REUNIÃO TÉCNICA DE PESQUISA E DESENVOLVIMENTO DA EMBRAPA AMAZÔNIA OCIDENTAL, 4., 2009, Manaus. Anais... Manaus: Embrapa Amazônia Ocidental, 2009. p. 57-60. (Embrapa Amazônia Ocidental. Documentos, 73).| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| Registros recuperados : 12 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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