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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
30/01/2013 |
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Data da última atualização: |
01/07/2016 |
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Autoria: |
LEITÃO, C. C. F.; COSTA, J. J. N.; SARAIVA, M. V. A.; ARAÚJO, V. R.; FIGUEIREDO, J. R.; HURK, R. van den; SILVA, J. R. V. |
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Título: |
Goat ovarian follicles express different levels of mRNA for inhibin-ßA subunit and activin-A stimulates secondary follicle growth in vitro. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Ciência Rural, Santa Maria, v. 43, n. 1, p. 107-113, jan., 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstracts: This study investigated the levels of messenger ribonucleic acids (mRNA) for inhibin-ßA subunit in goat primordial, primary and secondary follicles, as well as in cumulus-oocyte complexes (COCs) and mural granulosa / theca cells of antral follicles. The effects of activin-A (100ng mL-1) and/or follicle stimulating hormone (FSH, 50ng mL-1) on growth and expression of mRNA for activin-A and FSH receptor (FSH-R) in secondary follicles cultured for six days were evaluated. The data showed that the expression of inhibin-ßA is lower in secondary follicles than in primary follicles and is higher in large antral follicles than in small antral follicles. After culture, activin-A and/or FSH promoted growth of secondary follicles, while FSH increased the levels of mRNA for inhibin-ßA, and activin-A increased the levels of FSH-R mRNA. In conclusion, mRNA for inhibin-ßA is expressed at different levels in pre-antral and antral follicles and activin-A acts as a stimulator of the FSH-R expression in goat follicles. On its turn, the expression of inhibin-ßA is stimulated by FSH, which together with activin-A promotes secondary follicle growth in-vitro.
[Folículos ovarianos caprinos expressam diferentes níveis de RNAm para subunidade ßA da inibina e ativina-A promove o crescimento de folículos secundários in vitro].
Resumo: Este estudo investigou os níveis de ácidos ribonucleicos (RNAm) para a subunidade ßA da inibina em folículos primordiais, primários e secundários caprinos, bem como em complexos cumulus-oócitos (CCOs) e células da granulosa mural/teca de folículos antrais. Além disso, avaliaram-se os efeitos da ativina-A (100ng mL-1) e/ou hormônio folículo estimulante (FSH, 50ng mL-1) sobre o crescimento e a expressão do RNAm para inibina-ßA e receptores de FSH (FSH-R) em folículos secundários cultivados por seis dias. Os dados mostraram que a expressão de inibina-ßA é menor em folículos secundários do que em folículos primários e é maior em grandes folículos antrais que nos pequenos folículos antrais. Após o cultivo, ativina-A e/ou FSH promoveram o crescimento de folículos secundários. Enquanto o FSH aumentou os níveis de RNAm para inibina-ßA, a ativina-A aumentou os níveis de RNAm para FSH-R. Em conclusão, a inibina-ßA é expressa em diferentes níveis em folículos pré-antrais e antrais e a ativina-A atua como um estimulador da expressão de FSH-R em folículos caprinos. Por sua vez, a expressão de inibina-ßA é estimulada pelo FSH, que, juntamente com ativina, promove o crescimento de folículos secundários in vitro. MenosAbstracts: This study investigated the levels of messenger ribonucleic acids (mRNA) for inhibin-ßA subunit in goat primordial, primary and secondary follicles, as well as in cumulus-oocyte complexes (COCs) and mural granulosa / theca cells of antral follicles. The effects of activin-A (100ng mL-1) and/or follicle stimulating hormone (FSH, 50ng mL-1) on growth and expression of mRNA for activin-A and FSH receptor (FSH-R) in secondary follicles cultured for six days were evaluated. The data showed that the expression of inhibin-ßA is lower in secondary follicles than in primary follicles and is higher in large antral follicles than in small antral follicles. After culture, activin-A and/or FSH promoted growth of secondary follicles, while FSH increased the levels of mRNA for inhibin-ßA, and activin-A increased the levels of FSH-R mRNA. In conclusion, mRNA for inhibin-ßA is expressed at different levels in pre-antral and antral follicles and activin-A acts as a stimulator of the FSH-R expression in goat follicles. On its turn, the expression of inhibin-ßA is stimulated by FSH, which together with activin-A promotes secondary follicle growth in-vitro.
[Folículos ovarianos caprinos expressam diferentes níveis de RNAm para subunidade ßA da inibina e ativina-A promove o crescimento de folículos secundários in vitro].
Resumo: Este estudo investigou os níveis de ácidos ribonucleicos (RNAm) para a subunidade ßA da inibina em folículos primordiais, primários e secundários caprinos, be... Mostrar Tudo |
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Palavras-Chave: |
Folículos pré-antrais; Formação de antro; FSH-R; Oócito. |
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Thesagro: |
Caprino; Ovário. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03411naa a2200265 a 4500
001 1953855
005 2016-07-01
008 2013 bl uuuu u00u1 u #d
100 1 $aLEITÃO, C. C. F.
245 $aGoat ovarian follicles express different levels of mRNA for inhibin-ßA subunit and activin-A stimulates secondary follicle growth in vitro.
260 $c2013
520 $aAbstracts: This study investigated the levels of messenger ribonucleic acids (mRNA) for inhibin-ßA subunit in goat primordial, primary and secondary follicles, as well as in cumulus-oocyte complexes (COCs) and mural granulosa / theca cells of antral follicles. The effects of activin-A (100ng mL-1) and/or follicle stimulating hormone (FSH, 50ng mL-1) on growth and expression of mRNA for activin-A and FSH receptor (FSH-R) in secondary follicles cultured for six days were evaluated. The data showed that the expression of inhibin-ßA is lower in secondary follicles than in primary follicles and is higher in large antral follicles than in small antral follicles. After culture, activin-A and/or FSH promoted growth of secondary follicles, while FSH increased the levels of mRNA for inhibin-ßA, and activin-A increased the levels of FSH-R mRNA. In conclusion, mRNA for inhibin-ßA is expressed at different levels in pre-antral and antral follicles and activin-A acts as a stimulator of the FSH-R expression in goat follicles. On its turn, the expression of inhibin-ßA is stimulated by FSH, which together with activin-A promotes secondary follicle growth in-vitro. [Folículos ovarianos caprinos expressam diferentes níveis de RNAm para subunidade ßA da inibina e ativina-A promove o crescimento de folículos secundários in vitro]. Resumo: Este estudo investigou os níveis de ácidos ribonucleicos (RNAm) para a subunidade ßA da inibina em folículos primordiais, primários e secundários caprinos, bem como em complexos cumulus-oócitos (CCOs) e células da granulosa mural/teca de folículos antrais. Além disso, avaliaram-se os efeitos da ativina-A (100ng mL-1) e/ou hormônio folículo estimulante (FSH, 50ng mL-1) sobre o crescimento e a expressão do RNAm para inibina-ßA e receptores de FSH (FSH-R) em folículos secundários cultivados por seis dias. Os dados mostraram que a expressão de inibina-ßA é menor em folículos secundários do que em folículos primários e é maior em grandes folículos antrais que nos pequenos folículos antrais. Após o cultivo, ativina-A e/ou FSH promoveram o crescimento de folículos secundários. Enquanto o FSH aumentou os níveis de RNAm para inibina-ßA, a ativina-A aumentou os níveis de RNAm para FSH-R. Em conclusão, a inibina-ßA é expressa em diferentes níveis em folículos pré-antrais e antrais e a ativina-A atua como um estimulador da expressão de FSH-R em folículos caprinos. Por sua vez, a expressão de inibina-ßA é estimulada pelo FSH, que, juntamente com ativina, promove o crescimento de folículos secundários in vitro.
650 $aCaprino
650 $aOvário
653 $aFolículos pré-antrais
653 $aFormação de antro
653 $aFSH-R
653 $aOócito
700 1 $aCOSTA, J. J. N.
700 1 $aSARAIVA, M. V. A.
700 1 $aARAÚJO, V. R.
700 1 $aFIGUEIREDO, J. R.
700 1 $aHURK, R. van den
700 1 $aSILVA, J. R. V.
773 $tCiência Rural, Santa Maria$gv. 43, n. 1, p. 107-113, jan., 2013.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
10/02/2014 |
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Data da última atualização: |
19/10/2022 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
FRANZINI, V. I.; MENDES, F. L.; MURAOKA, T.; TREVISAM, A. R.; ADU-GYAMFI, J. J. |
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Afiliação: |
VINICIUS IDE FRANZINI, CPATU; CPATU; CENA/USP; CENA/USP; International Atomic Energy Agency. |
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Título: |
Biological nitrogen fixation efficiency in brazilian common bean genotypes as measured by 15N methodology. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In: OPTIMIZING productivity of food crop genotypes in low nutrient soils. Vienna: FAO: IAEA, 2013. |
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Páginas: |
p. 299-309. |
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Série: |
(IAEA Tecdoc, 1721). |
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Idioma: |
Inglês |
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Conteúdo: |
Common bean (Phaseolus vulgaris L.) represents the main source of protein for the Brazilian and other Latin-American populations. Unlike soybean, which is very efficient in fixing atmospheric N2 symbiotically, common bean does not dispense with the need for N fertilizer application, as the biologically fixed N (BNF) seems incapable to supplement the total N required by the crop. A experiment under controlled conditions was conducted in Piracicaba, Brazil, to assess N2 fixation of 25 genotypes of common bean (Phaseolus vulgaris L.). BNF was measured by 15N isotope dilution using a non-N2 fixing bean genotype as a reference crop. The common bean genotypes were grown in low (2.2 mg N kg-1 soil) or high N content soil (200 mg N kg-1 soil), through N fertilizer application, as urea-15N (31.20 and 1.4 atom % 15N, respectively). The bean seeds were inoculated with Rhizobium tropici CIAT 899 strain and the plants were harvested at grain maturity stage. The contribution of BNF was on average 75% of total plant N content, and there were differences in N fixing capacity among the bean genotypes. The most efficient genotypes were Horizonte, Roxo 90, Grafite, Aporé and Vereda, when grown in high N soil. None of the genotypes grown in low N soil was efficient in producing grains compared to those grown in high N soil, and therefore the BNF was not able to supply the total N demand of the bean crop. |
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Thesagro: |
Feijão; Genótipo; Nitrogênio. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/96865/1/p299.pdf
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Marc: |
LEADER 02128naa a2200229 a 4500
001 1979298
005 2022-10-19
008 2013 bl uuuu u00u1 u #d
100 1 $aFRANZINI, V. I.
245 $aBiological nitrogen fixation efficiency in brazilian common bean genotypes as measured by 15N methodology.$h[electronic resource]
260 $c2013
300 $ap. 299-309.
490 $a(IAEA Tecdoc, 1721).
520 $aCommon bean (Phaseolus vulgaris L.) represents the main source of protein for the Brazilian and other Latin-American populations. Unlike soybean, which is very efficient in fixing atmospheric N2 symbiotically, common bean does not dispense with the need for N fertilizer application, as the biologically fixed N (BNF) seems incapable to supplement the total N required by the crop. A experiment under controlled conditions was conducted in Piracicaba, Brazil, to assess N2 fixation of 25 genotypes of common bean (Phaseolus vulgaris L.). BNF was measured by 15N isotope dilution using a non-N2 fixing bean genotype as a reference crop. The common bean genotypes were grown in low (2.2 mg N kg-1 soil) or high N content soil (200 mg N kg-1 soil), through N fertilizer application, as urea-15N (31.20 and 1.4 atom % 15N, respectively). The bean seeds were inoculated with Rhizobium tropici CIAT 899 strain and the plants were harvested at grain maturity stage. The contribution of BNF was on average 75% of total plant N content, and there were differences in N fixing capacity among the bean genotypes. The most efficient genotypes were Horizonte, Roxo 90, Grafite, Aporé and Vereda, when grown in high N soil. None of the genotypes grown in low N soil was efficient in producing grains compared to those grown in high N soil, and therefore the BNF was not able to supply the total N demand of the bean crop.
650 $aFeijão
650 $aGenótipo
650 $aNitrogênio
700 1 $aMENDES, F. L.
700 1 $aMURAOKA, T.
700 1 $aTREVISAM, A. R.
700 1 $aADU-GYAMFI, J. J.
773 $tIn: OPTIMIZING productivity of food crop genotypes in low nutrient soils. Vienna: FAO: IAEA, 2013.
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