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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
08/02/2017 |
Data da última atualização: |
16/05/2022 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
OLIVARES, C.; FONSECA, J. F. da; SARAIVA, H.; ALFRADIQUE, V.; CÔRTES, L.; COSTA, L.; BRANDÃO, F.; SOUZA-FABJAN, J. M. |
Afiliação: |
Carolina Olivares; JEFERSON FERREIRA DA FONSECA, CNPC; Helena Saraiva; Vivian Alfradique; Luana Côrtes; Lendel Costa; Felipe Brandão; Joanna Maria Souza-Fabjan. |
Título: |
Influence of Mini-Percoll techniques on motility parameters of ram frozen-thawed sperm. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
In: INTERNATIONAL CONGRESS OF ANIMAL REPRODUCTION, 18., 2016, Tours. Abstracts... Tous: Society for Reprocutive Biololy, 2016. p. 168-169. Abstract PW171 |
Descrição Física: |
Poster. |
Idioma: |
Inglês |
Conteúdo: |
The aim of this study was to compare the influence of different forces and time of centrifugation in mini-Percoll techniques with traditional Percoll for sperm selection on motility parameters of ram frozen-thawed sperm. For the first time, we have demonstrated that the reduction of the gradient volume and time of centrifugation, and the increase of its force at Percoll, can be successfully used for ram sperm selection. In conclusion, mini-Percoll may be used instead of traditional Percoll, decreasing costs and time of sperm handling, without motility damages in ram frozen-thawed sperm. |
Palavras-Chave: |
Cinética espermática; Força e tempo de centrifugação; Gradiente de Percoll; Motilidade espermática; Movemen; Seleção espermática; Sexagem; Sperm quality. |
Thesagro: |
Carneiro; Espermatozóide; Ovino; Reprodução animal; Sêmen. |
Thesaurus Nal: |
Rams; Sexual reproduction; Sheep; Spermatozoa. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/155074/1/cnpc-2016-Influence.pdf
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Marc: |
LEADER 01828nam a2200409 a 4500 001 2063221 005 2022-05-16 008 2016 bl uuuu u00u1 u #d 100 1 $aOLIVARES, C. 245 $aInfluence of Mini-Percoll techniques on motility parameters of ram frozen-thawed sperm.$h[electronic resource] 260 $aIn: INTERNATIONAL CONGRESS OF ANIMAL REPRODUCTION, 18., 2016, Tours. Abstracts... Tous: Society for Reprocutive Biololy, 2016. p. 168-169. Abstract PW171$c2016 300 $cPoster. 520 $aThe aim of this study was to compare the influence of different forces and time of centrifugation in mini-Percoll techniques with traditional Percoll for sperm selection on motility parameters of ram frozen-thawed sperm. For the first time, we have demonstrated that the reduction of the gradient volume and time of centrifugation, and the increase of its force at Percoll, can be successfully used for ram sperm selection. In conclusion, mini-Percoll may be used instead of traditional Percoll, decreasing costs and time of sperm handling, without motility damages in ram frozen-thawed sperm. 650 $aRams 650 $aSexual reproduction 650 $aSheep 650 $aSpermatozoa 650 $aCarneiro 650 $aEspermatozóide 650 $aOvino 650 $aReprodução animal 650 $aSêmen 653 $aCinética espermática 653 $aForça e tempo de centrifugação 653 $aGradiente de Percoll 653 $aMotilidade espermática 653 $aMovemen 653 $aSeleção espermática 653 $aSexagem 653 $aSperm quality 700 1 $aFONSECA, J. F. da 700 1 $aSARAIVA, H. 700 1 $aALFRADIQUE, V. 700 1 $aCÔRTES, L. 700 1 $aCOSTA, L. 700 1 $aBRANDÃO, F. 700 1 $aSOUZA-FABJAN, J. M.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
10/01/2019 |
Data da última atualização: |
10/01/2019 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
PÉREZ-JARAMILLO, J. E.; CARRION, V. J.; HOLLANDER, M. de; MENDES, R.; RAAIJMAKERS, J. M. |
Afiliação: |
J.E. Pérez-Jaramillo, Netherlands Institute of Ecology NIOO-KNAW, Microbial Ecology, Wageningen, The Netherlands; V. J. Carrion, Netherlands Institute of Ecology NIOO-KNAW, Microbial Ecology, Wageningen, The Netherlands; M. de HOLLANDER, Netherlands Institute of Ecology NIOO-KNAW, Microbial Ecology, Wageningen, The Netherlands; RODRIGO MENDES, CNPMA; J. M. RAAIJMAKERS, Netherlands Institute of Ecology NIOO-KNAW, Microbial Ecology, Wageningen, The Netherlands. |
Título: |
The spermosphere effect: building up plant microbiome assembly. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
In: CONGRESS OF EUROPEAN MICROBIOLOGISTS - FEMS, 7., 2017, Valencia, Spain. Abstract Book... Valencia: Federation of European Microbiological Society (FEMS), 2017. Ref. FEMS7-2470. |
Idioma: |
Português |
Conteúdo: |
Plants have a significant influence on the diversity and activity of soil microbial communities. During imbibition and germination, plant seeds release chemically diverse exudates thereby promoting microbial activity in the zone surrounding the seed, also referred to as the spermosphere. To date, little is known about the diversity and activities of microbial communities in the spermosphere and how this short-lived plant developmental stage affects microbiome assembly. Here, we deciphered the magnitude of the spermosphere effect for two different food crops, i.e. tomato and bean. More specifically, we investigated if a plant genotype-dependent influence is discernible in the spermosphere and to what extent the spermosphere microbiome relates to the rhizosphere microbiome. We selected wild and modern accessions of tomato and common bean for which strong differences in the rhizosphere microbiome were found in previous studies. Community profiling of the spermosphere revealed a decrease of ?-diversity of all crop accessions as compared to the bulk soil. Similarly, a significant difference in the ?-diversity was observed between bean accessions and bulk soil. Bacteroidetes, Firmicutes and Proteobacteria were the bacterial phyla that consistently responded to the seed germination and were significantly more abundant in the spermosphere. Albeit small, significant differences in the ?-diversity were detected between wild and modern crop accessions, suggesting a plant genotype-dependent effect already at this early developmental stage. If and how seed exudates are the main driver of the differences in spermosphere communities between the wild and modern crop accessions is currently under investigation. MenosPlants have a significant influence on the diversity and activity of soil microbial communities. During imbibition and germination, plant seeds release chemically diverse exudates thereby promoting microbial activity in the zone surrounding the seed, also referred to as the spermosphere. To date, little is known about the diversity and activities of microbial communities in the spermosphere and how this short-lived plant developmental stage affects microbiome assembly. Here, we deciphered the magnitude of the spermosphere effect for two different food crops, i.e. tomato and bean. More specifically, we investigated if a plant genotype-dependent influence is discernible in the spermosphere and to what extent the spermosphere microbiome relates to the rhizosphere microbiome. We selected wild and modern accessions of tomato and common bean for which strong differences in the rhizosphere microbiome were found in previous studies. Community profiling of the spermosphere revealed a decrease of ?-diversity of all crop accessions as compared to the bulk soil. Similarly, a significant difference in the ?-diversity was observed between bean accessions and bulk soil. Bacteroidetes, Firmicutes and Proteobacteria were the bacterial phyla that consistently responded to the seed germination and were significantly more abundant in the spermosphere. Albeit small, significant differences in the ?-diversity were detected between wild and modern crop accessions, suggesting a plant genotype-depen... Mostrar Tudo |
Thesaurus NAL: |
Beans; Microbiome; Tomatoes. |
Categoria do assunto: |
V Taxonomia de Organismos |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/190199/1/RA-MendesR-FEMS-2017-Ref-FEMS7-2470.pdf
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Marc: |
LEADER 02408nam a2200193 a 4500 001 2103544 005 2019-01-10 008 2018 bl uuuu u00u1 u #d 100 1 $aPÉREZ-JARAMILLO, J. E. 245 $aThe spermosphere effect$bbuilding up plant microbiome assembly.$h[electronic resource] 260 $aIn: CONGRESS OF EUROPEAN MICROBIOLOGISTS - FEMS, 7., 2017, Valencia, Spain. Abstract Book... Valencia: Federation of European Microbiological Society (FEMS), 2017. Ref. FEMS7-2470.$c2470 520 $aPlants have a significant influence on the diversity and activity of soil microbial communities. During imbibition and germination, plant seeds release chemically diverse exudates thereby promoting microbial activity in the zone surrounding the seed, also referred to as the spermosphere. To date, little is known about the diversity and activities of microbial communities in the spermosphere and how this short-lived plant developmental stage affects microbiome assembly. Here, we deciphered the magnitude of the spermosphere effect for two different food crops, i.e. tomato and bean. More specifically, we investigated if a plant genotype-dependent influence is discernible in the spermosphere and to what extent the spermosphere microbiome relates to the rhizosphere microbiome. We selected wild and modern accessions of tomato and common bean for which strong differences in the rhizosphere microbiome were found in previous studies. Community profiling of the spermosphere revealed a decrease of ?-diversity of all crop accessions as compared to the bulk soil. Similarly, a significant difference in the ?-diversity was observed between bean accessions and bulk soil. Bacteroidetes, Firmicutes and Proteobacteria were the bacterial phyla that consistently responded to the seed germination and were significantly more abundant in the spermosphere. Albeit small, significant differences in the ?-diversity were detected between wild and modern crop accessions, suggesting a plant genotype-dependent effect already at this early developmental stage. If and how seed exudates are the main driver of the differences in spermosphere communities between the wild and modern crop accessions is currently under investigation. 650 $aBeans 650 $aMicrobiome 650 $aTomatoes 700 1 $aCARRION, V. J. 700 1 $aHOLLANDER, M. de 700 1 $aMENDES, R. 700 1 $aRAAIJMAKERS, J. M.
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Embrapa Meio Ambiente (CNPMA) |
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