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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
19/04/2023 |
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Data da última atualização: |
19/04/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RIEGER, J. S. G.; MANTOVANI, C.; SUNIGA, P. A. P.; PINTO, I. B.; SOUSA, G. A. A.; GAVA, D.; CANTAO, M. E.; SANTOS, L. R.; ARAÚJO, F. R.; ZANELLA, J. R. C. |
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Afiliação: |
DANIELLE GAVA, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA; JANICE REIS CIACCI ZANELLA, CNPSA. |
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Título: |
Standardization of ELISA with senecavirus A recombinant VP2 protein and its use in swine herds in Brazil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 22, n. 1, ed. gmr19118, 2023. |
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DOI: |
http://dx.doi.org/10.4238/gmr19118 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Senecavirus A (SVA) is a nonenveloped, single-stranded RNA virusThe icosahedral viral particle is composed of four structural proteins: VP1, VP2, VP3 and VP4, among which VP2 is strongly involved in the antibody immune response. The virus causes vesicles on the snout and feet in pigs, which are clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. Outbreaks of SVA have been reported worldwide since 2014; however, its prevalence in Brazil remains unknown. In this study, the VP2 structural protein was produced and purified from E. coli, and recombinant VP2 (rVP2), based on the most recent Brazilian strain, was used to develop an indirect ELISA to identify antibodies against SVA in Brazilian swine herds. Sensitivity and specificity values of the rVP2 ELISA were determined using receiver operating characteristic analysis performed on 43 SVA positive and 219 negative serum samples. In addition, serum samples from pigs immunized with eight distinct Brazilian SVA inactivated strains were tested with the rVP2 ELISA. For the specificity of the assay, 17 serum samples from vesicular stomatitis virus (VSV) from naturally infected pigs were tested. The rVP2 ELISA was found to have 100% specificity and 74.4% sensitivity. The performance of the assay using samples collected during the SVA outbreak, had a sensitivity of 100%, and with those collected nine months after the outbreak it had a sensitivity of 73.4%. The rVP2 ELISA developed here was able to detect specific SVA antibodies in acute disease and recovered pigs, and no cross-reactivity with VSV was observed. This assay has potential as a useful tool for monitoring SVA infection and could help to improve disease diagnosis. MenosAbstract: Senecavirus A (SVA) is a nonenveloped, single-stranded RNA virusThe icosahedral viral particle is composed of four structural proteins: VP1, VP2, VP3 and VP4, among which VP2 is strongly involved in the antibody immune response. The virus causes vesicles on the snout and feet in pigs, which are clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. Outbreaks of SVA have been reported worldwide since 2014; however, its prevalence in Brazil remains unknown. In this study, the VP2 structural protein was produced and purified from E. coli, and recombinant VP2 (rVP2), based on the most recent Brazilian strain, was used to develop an indirect ELISA to identify antibodies against SVA in Brazilian swine herds. Sensitivity and specificity values of the rVP2 ELISA were determined using receiver operating characteristic analysis performed on 43 SVA positive and 219 negative serum samples. In addition, serum samples from pigs immunized with eight distinct Brazilian SVA inactivated strains were tested with the rVP2 ELISA. For the specificity of the assay, 17 serum samples from vesicular stomatitis virus (VSV) from naturally infected pigs were tested. The rVP2 ELISA was found to have 100% specificity and 74.4% sensitivity. The performance of the assay using samples collected during the SVA outbreak, had a sensitivity of 100%, and with those collected nine months after the outbreak it had a sensitivity of 73.4%. The rVP2 ELISA developed here wa... Mostrar Tudo |
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Thesagro: |
Diagnostico. |
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Thesaurus Nal: |
Senecavirus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1153239/1/final10100.pdf
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Marc: |
LEADER 02545naa a2200265 a 4500
001 2153239
005 2023-04-19
008 2023 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4238/gmr19118$2DOI
100 1 $aRIEGER, J. S. G.
245 $aStandardization of ELISA with senecavirus A recombinant VP2 protein and its use in swine herds in Brazil.$h[electronic resource]
260 $c2023
520 $aAbstract: Senecavirus A (SVA) is a nonenveloped, single-stranded RNA virusThe icosahedral viral particle is composed of four structural proteins: VP1, VP2, VP3 and VP4, among which VP2 is strongly involved in the antibody immune response. The virus causes vesicles on the snout and feet in pigs, which are clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. Outbreaks of SVA have been reported worldwide since 2014; however, its prevalence in Brazil remains unknown. In this study, the VP2 structural protein was produced and purified from E. coli, and recombinant VP2 (rVP2), based on the most recent Brazilian strain, was used to develop an indirect ELISA to identify antibodies against SVA in Brazilian swine herds. Sensitivity and specificity values of the rVP2 ELISA were determined using receiver operating characteristic analysis performed on 43 SVA positive and 219 negative serum samples. In addition, serum samples from pigs immunized with eight distinct Brazilian SVA inactivated strains were tested with the rVP2 ELISA. For the specificity of the assay, 17 serum samples from vesicular stomatitis virus (VSV) from naturally infected pigs were tested. The rVP2 ELISA was found to have 100% specificity and 74.4% sensitivity. The performance of the assay using samples collected during the SVA outbreak, had a sensitivity of 100%, and with those collected nine months after the outbreak it had a sensitivity of 73.4%. The rVP2 ELISA developed here was able to detect specific SVA antibodies in acute disease and recovered pigs, and no cross-reactivity with VSV was observed. This assay has potential as a useful tool for monitoring SVA infection and could help to improve disease diagnosis.
650 $aSenecavirus
650 $aDiagnostico
700 1 $aMANTOVANI, C.
700 1 $aSUNIGA, P. A. P.
700 1 $aPINTO, I. B.
700 1 $aSOUSA, G. A. A.
700 1 $aGAVA, D.
700 1 $aCANTAO, M. E.
700 1 $aSANTOS, L. R.
700 1 $aARAÚJO, F. R.
700 1 $aZANELLA, J. R. C.
773 $tGenetics and Molecular Research$gv. 22, n. 1, ed. gmr19118, 2023.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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| Registros recuperados : 20.759 | |
| 182. |  | LINS-TEIXEIRA, A.; TAPIA-CORAL, S. C.; LUIZÃO, F. J.; MORAIS, J. W.; WANDELLI, E. V. Macro-invertebrados do solo em capoeiras trituradas usadas como alternativa ao uso do fogo no Assentamento Tarumã-Mirim, Amazonas. In: CONGRESSO DE ECOLOGIA DO BRASIL, 8., 2007, Caxambú. Anais... [S.l.]: Sociedade de Ecologia do Brasil, 2007. p. 1-2.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 184. | | SPERA, S. T.; SANTOS, H. P. dos; FONTANELI, R. S.; TOMM, G. O.; PAZA, A. P.; SIGNOR, L. R. Efeito de sistemas de manejo do solo nos atributos físicos de solo, após vinte anos. In: COSTAMILAN, L. M.; BERTAGNOLLI, P. F. (Org.). Soja: resultados de pesquisa 2007/2008. Passo Fundo: Embrapa Trigo, 2008. p. 127-139. (Embrapa Trigo. Documentos, 86).| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Trigo. |
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| 185. | | SANTOS, H. P. dos; FONTANELI, R. S.; SPERA, S. T.; TOMM, G. O.; SANTOS, D. B. Efeito de sistemas de manejo do solo na fertilidade e no nível de matéria orgânica do solo, após vinte anos. In: REUNIÃO TÉCNICA ANUAL DE MILHO, 53., REUNIÃO TÉCNICA ANUAL DE SORGO, 36., 2008. Pelotas. Atas e resumos... Pelotas: Embrapa Clima Temperado, 2008. 1 CD-ROM. 6 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| 189. | | ALVES, V. B.; CECCON, G.; MATOSO, A. de O.; PADILHA, N. de S.; LUIS NETO NETO, A. Crescimento inicial de espécies forrageiras anuais e perenes em solo com dois níveis de fertilidade. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 30.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 14.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 12.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 9.; SIMPÓSIO SOBRE SELÊNIO NO BRASIL, 1., 2012, Maceió. A responsabilidade socioambiental da pesquisa agrícola: anais. Viçosa: SBCS, 2012. Fertbio. Organizado por Leila Cruz da Silva, Gilson Moura Filho, Adriano Barboza Moura, Abel Washington de Albuquerque.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agropecuária Oeste. |
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| 190. | | SOUZA, R. C.; CANTÃO, M. E.; NOGUEIRA, M. A.; VANSCONCELOS, A. T. R.; BARCELLOS, F. G.; VICENTE, V. A.; HUNGRIA, M. Diversidade funcional de genes com potencial biotecnológico em um solo agrícola no Norte do Paraná. In: CONGRESSO BRASILEIRO MICROBIOLOGIA, 27., SIMPÓSIO IBEROAMERICANO SOBRE MICRO-ORGANISMOS FOTOSSINTETIZANTES, 2.; SIMPÓSIO BRASILEIRO DE MICOBACTÉRIAS, 15.; SIMPÓSIO DE FERMENTAÇÃO ALCOÓLICA, 2.; BRAZILIAN MICROBIOME WORKSHOP; BRAZILIAN MICROBIOME PROJECT MEETING, 1.; SIMPÓSIO DE COLEÇÕES DE CULTURA, 4.; MINI-SIMPÓSIO SOBRE NEW DELHI METALO-BETA-LACTAMASE-1 (NDM-1); 2013, NATAL. Anais... [São Paulo]: Sociedade Brasileira de Microbiologia, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 197. | | MACEDO, R. S.; TEIXEIRA, W. G.; MARTINS, G. C.; SOUZA, A. C. G. de; ENCINAS, O. C. Dinâmica do carbono e nutrientes em solos sob floresta primária e em áreas com diferentes idades de reflorestamento na Província Petrolífera de Urucu, Coari-AM. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 32., 2009, Fortaleza. O solo e a produção de bioenergia: perspectivas e desafios : resumos. [Viçosa, MG]: SBCS; Fortaleza: UFC, 2009. p. 535. PT2573.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 200. | | LIMA FILHO, O. F. de; SILVA, C. J. da; MOUTA, E. R. Efeito da aplicação de escória de siderurgia granulada em cana-soca junto com a adubação NPK. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 29.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 13.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 11.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 8., 2010, Guarapari. Fontes de nutrientes e produção agrícola: modelando o futuro: anais. Viçosa, MG: SBCS, 2010. 1 CD-ROM. FERTBIO 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agropecuária Oeste. |
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| Registros recuperados : 20.759 | |
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