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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
19/04/2023 |
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Data da última atualização: |
19/04/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RIEGER, J. S. G.; MANTOVANI, C.; SUNIGA, P. A. P.; PINTO, I. B.; SOUSA, G. A. A.; GAVA, D.; CANTAO, M. E.; SANTOS, L. R.; ARAÚJO, F. R.; ZANELLA, J. R. C. |
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Afiliação: |
DANIELLE GAVA, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA; JANICE REIS CIACCI ZANELLA, CNPSA. |
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Título: |
Standardization of ELISA with senecavirus A recombinant VP2 protein and its use in swine herds in Brazil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 22, n. 1, ed. gmr19118, 2023. |
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DOI: |
http://dx.doi.org/10.4238/gmr19118 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Senecavirus A (SVA) is a nonenveloped, single-stranded RNA virusThe icosahedral viral particle is composed of four structural proteins: VP1, VP2, VP3 and VP4, among which VP2 is strongly involved in the antibody immune response. The virus causes vesicles on the snout and feet in pigs, which are clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. Outbreaks of SVA have been reported worldwide since 2014; however, its prevalence in Brazil remains unknown. In this study, the VP2 structural protein was produced and purified from E. coli, and recombinant VP2 (rVP2), based on the most recent Brazilian strain, was used to develop an indirect ELISA to identify antibodies against SVA in Brazilian swine herds. Sensitivity and specificity values of the rVP2 ELISA were determined using receiver operating characteristic analysis performed on 43 SVA positive and 219 negative serum samples. In addition, serum samples from pigs immunized with eight distinct Brazilian SVA inactivated strains were tested with the rVP2 ELISA. For the specificity of the assay, 17 serum samples from vesicular stomatitis virus (VSV) from naturally infected pigs were tested. The rVP2 ELISA was found to have 100% specificity and 74.4% sensitivity. The performance of the assay using samples collected during the SVA outbreak, had a sensitivity of 100%, and with those collected nine months after the outbreak it had a sensitivity of 73.4%. The rVP2 ELISA developed here was able to detect specific SVA antibodies in acute disease and recovered pigs, and no cross-reactivity with VSV was observed. This assay has potential as a useful tool for monitoring SVA infection and could help to improve disease diagnosis. MenosAbstract: Senecavirus A (SVA) is a nonenveloped, single-stranded RNA virusThe icosahedral viral particle is composed of four structural proteins: VP1, VP2, VP3 and VP4, among which VP2 is strongly involved in the antibody immune response. The virus causes vesicles on the snout and feet in pigs, which are clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. Outbreaks of SVA have been reported worldwide since 2014; however, its prevalence in Brazil remains unknown. In this study, the VP2 structural protein was produced and purified from E. coli, and recombinant VP2 (rVP2), based on the most recent Brazilian strain, was used to develop an indirect ELISA to identify antibodies against SVA in Brazilian swine herds. Sensitivity and specificity values of the rVP2 ELISA were determined using receiver operating characteristic analysis performed on 43 SVA positive and 219 negative serum samples. In addition, serum samples from pigs immunized with eight distinct Brazilian SVA inactivated strains were tested with the rVP2 ELISA. For the specificity of the assay, 17 serum samples from vesicular stomatitis virus (VSV) from naturally infected pigs were tested. The rVP2 ELISA was found to have 100% specificity and 74.4% sensitivity. The performance of the assay using samples collected during the SVA outbreak, had a sensitivity of 100%, and with those collected nine months after the outbreak it had a sensitivity of 73.4%. The rVP2 ELISA developed here wa... Mostrar Tudo |
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Thesagro: |
Diagnostico. |
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Thesaurus Nal: |
Senecavirus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1153239/1/final10100.pdf
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Marc: |
LEADER 02545naa a2200265 a 4500
001 2153239
005 2023-04-19
008 2023 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4238/gmr19118$2DOI
100 1 $aRIEGER, J. S. G.
245 $aStandardization of ELISA with senecavirus A recombinant VP2 protein and its use in swine herds in Brazil.$h[electronic resource]
260 $c2023
520 $aAbstract: Senecavirus A (SVA) is a nonenveloped, single-stranded RNA virusThe icosahedral viral particle is composed of four structural proteins: VP1, VP2, VP3 and VP4, among which VP2 is strongly involved in the antibody immune response. The virus causes vesicles on the snout and feet in pigs, which are clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. Outbreaks of SVA have been reported worldwide since 2014; however, its prevalence in Brazil remains unknown. In this study, the VP2 structural protein was produced and purified from E. coli, and recombinant VP2 (rVP2), based on the most recent Brazilian strain, was used to develop an indirect ELISA to identify antibodies against SVA in Brazilian swine herds. Sensitivity and specificity values of the rVP2 ELISA were determined using receiver operating characteristic analysis performed on 43 SVA positive and 219 negative serum samples. In addition, serum samples from pigs immunized with eight distinct Brazilian SVA inactivated strains were tested with the rVP2 ELISA. For the specificity of the assay, 17 serum samples from vesicular stomatitis virus (VSV) from naturally infected pigs were tested. The rVP2 ELISA was found to have 100% specificity and 74.4% sensitivity. The performance of the assay using samples collected during the SVA outbreak, had a sensitivity of 100%, and with those collected nine months after the outbreak it had a sensitivity of 73.4%. The rVP2 ELISA developed here was able to detect specific SVA antibodies in acute disease and recovered pigs, and no cross-reactivity with VSV was observed. This assay has potential as a useful tool for monitoring SVA infection and could help to improve disease diagnosis.
650 $aSenecavirus
650 $aDiagnostico
700 1 $aMANTOVANI, C.
700 1 $aSUNIGA, P. A. P.
700 1 $aPINTO, I. B.
700 1 $aSOUSA, G. A. A.
700 1 $aGAVA, D.
700 1 $aCANTAO, M. E.
700 1 $aSANTOS, L. R.
700 1 $aARAÚJO, F. R.
700 1 $aZANELLA, J. R. C.
773 $tGenetics and Molecular Research$gv. 22, n. 1, ed. gmr19118, 2023.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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| Registros recuperados : 173 | |
| 1. |  | SANTOS, L. R. P. Cuias ameaçadas. Revista Globo Rural, São Paulo, v. 23, n. 273, p. 31, jul. 2008. Informações prestadas por Marina Castelo Branco e Gilmar Paulo Henz, pesquisadores da Embrapa Hortaliças.| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Hortaliças. |
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| 7. | | SANTOS, L. R. dos; GASPAR, E. B. Vacina sim: na medicina veterinária também. Disponível em: https://www.embrapa.br/busca-de-noticias/-/noticia/64113417/artigo----vacina-sim-na-medicina-veterinaria-tambem. Acesso em: 21 out. 2021. Disponibilizado também em: https://www.agorams.com.br/vacina-sim-na-medicina-veterinaria-tambem/| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 10. | | RIBEIRO, S. M.; ROSINHA, G. M. S.; SANTOS, L. R. dos; SANTOS, G. M. Avaliação da resposta imune de camundongos BALB/c a uma proteína recombinante visando o desenvolvimento de vacina contra tristeza parasitária bovina. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 11., 2015, Campo Grande, MS. Anais... Campo Grande: Embrapa Gado de Corte, 2015. 114 p. (Embrapa Gado de Corte. Documentos, 213).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 16. | | SANTOS, L. R. R. dos; LEMOS, O. F. de; RODRIGUES, S. de M. Germinação in vitro de diferentes cultivares de pimenta-do-reino (Piper nigrum L.). In: SEMINÁRIO CIENTÍFICO DA UFRA, 7.; SEMINÁRIO [DE INICIAÇÃO CIENTÍFICA] DA EMBRAPA AMAZÔNIA ORIENTAL, 13.; SEMINÁRIO DE PESQUISA DA UFRA, 1., 2009, Belém, PA. Pesquisa e desenvolvimento tecnológico na formação do jovem cientista: anais. Belém, PA: UFRA: Embrapa Amazônia Oriental, 2009. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 18. | | CAMPELO, M. F.; LEMOS, O. F. de; SANTOS, L. R. R. dos; MIRANDA, S. de R. Proliferação de brotos de cultivares de bananeira em cinco subcultivos em diferentes concentrações de benzilaminopurina a cada subcultivo. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA ORIENTAL, 15., 2011, Belém, PA. A ciência de fazer ciência: anais. Belém, PA: Embrapa Amazônia Oriental, 2011. 1 CD-ROM. PIBIC-2011.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 19. | | SANTOS, L. R. R. dos; LEMOS, O. F. de; RODRIGUES, S. de M.; MONDIN, M. Quantidade de DNA nuclear de genótipos do gênero Piper. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA, 19.; SEMINÁRIO DE PÓS-GRADUAÇÃO DA EMBRAPA AMAZÔNIA ORIENTAL, 3., 2015, Belém, PA. Anais. Belém, PA: Embrapa Amazônia Oriental, 2015. p. 331-334.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 20. | | SANTOS, L. R. dos; ZANGIROLAMI, M. de S.; SILVA, N. O.; VALDERRAMA, P.; MARÇO, P. H. Rapid non-invasive assessment of quality parameters in ground soybean using near-infrared spectroscopy. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 53, n. 1, p. 97-104, jan. 2018. Título em português: Avaliação rápida não invasiva de parâmetros de qualidade em soja triturada com uso de espectroscopia de infravermelho próximo.| Biblioteca(s): Embrapa Unidades Centrais. |
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| Registros recuperados : 173 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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