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Registro Completo |
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
19/12/2023 |
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Data da última atualização: |
29/12/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, K. S.; COSTA, C.; BESSA, M. J.; TEIXEIRA, J. P.; SILVA, A. V. C. da; PADILHA, F. F.; DARIVA, C.; OLIVEIRA, M. B. P. P. |
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Afiliação: |
KLEBSON SILVA SANTOS, ITP; CARLA COSTA, Portuguese National Institute of Health; MARIA JOÃO BESSA, Universidade do Porto; JOAO PAULO TEIXEIRA, UNIVERSIDADE DO PORTO; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC; FRANCINE FERREIRA PADILHA, ITP; CLÁUDIO DARIVA, ITP; MARIA BEATRIZ PINTO PRIOR OLIVEIRA, UNIVERSIDADE DO PORTO. |
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Título: |
Azadirachta indica A. Juss (neem) phenolic extract inhibits human B-lymphoblastoid cells growth via cell cycle arrest, apoptosis induction, and DNA damage. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Exploration of Foods and Foodomics, v. 1, p. 130-142, 2023. |
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DOI: |
https://doi.org/10.37349/eff.2023.00011 |
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Idioma: |
Inglês |
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Conteúdo: |
Aim: As far as is known, the pharmaceutical effects of neem on human B-lymphoblastoid (TK6) cells have not been studied until now. Hence, the present study aimed to obtain neem phenolic extracts for inhibits the proliferation of TK6 cells and explore some possible underlying mechanisms involved in these effects. Methods: Hexane extract (HE) was obtained in the first step. After that, the residual hexane was removed
from the neem. The dried neem sample was used in a new extraction for obtaining the ethyl acetate extract (EAE). Total phenolic compounds (TPC) and total flavonoid contents (TFC) were determined by spectrophotometric methods. Lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) tests were used to evaluate the cytotoxicity in TK6 cells. The stop at G0/G1 cell cycle and inducing apoptosis in the TK6 cells were analyzed by flow cytometry. For deoxyribonucleic acid (DNA) damage evaluation, the alkaline comet test was used. Results: The higher TFC (65.50 mg/g of extract ± 1.17 mg/g of extract) and TPC (52.08 mg of extract ± 0.88 mg of extract) were obtained in EAE compared to HE that was obtained TFC of 14.61 mg/g of extract ± 0.60 mg/g of extract and TPC of 3.20 mg/g of extract ± 1.20 mg/g of extract. EAE was more significantly cytotoxic to TK6 cells than HE. The apoptosis induction was higher after exposure to 15.0 μg/mL of EAE (11.29%) in comparison to 15.0 μg/mL of HE (2.52%). The G0/G1 phase increased from 72% negative control (NC) to 83% after treatment with neem extracts (15 μg/mL). Neem extracts were also able to cause DNA strand breaks in TK6 cells. MenosAim: As far as is known, the pharmaceutical effects of neem on human B-lymphoblastoid (TK6) cells have not been studied until now. Hence, the present study aimed to obtain neem phenolic extracts for inhibits the proliferation of TK6 cells and explore some possible underlying mechanisms involved in these effects. Methods: Hexane extract (HE) was obtained in the first step. After that, the residual hexane was removed
from the neem. The dried neem sample was used in a new extraction for obtaining the ethyl acetate extract (EAE). Total phenolic compounds (TPC) and total flavonoid contents (TFC) were determined by spectrophotometric methods. Lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) tests were used to evaluate the cytotoxicity in TK6 cells. The stop at G0/G1 cell cycle and inducing apoptosis in the TK6 cells were analyzed by flow cytometry. For deoxyribonucleic acid (DNA) damage evaluation, the alkaline comet test was used. Results: The higher TFC (65.50 mg/g of extract ± 1.17 mg/g of extract) and TPC (52.08 mg of extract ± 0.88 mg of extract) were obtained in EAE compared to HE that was obtained TFC of 14.61 mg/g of extract ± 0.60 mg/g of extract and TPC of 3.20 mg/g of extract ± 1.20 mg/g of extract. EAE was more significantly cytotoxic to TK6 cells than HE. The apoptosis induction was higher after exposure to 15.0 μg/mL of EAE (11.29%) in comparison to 15.0 μg/mL of HE (2.52%). The G0/G1 phase increased from 72% negati... Mostrar Tudo |
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Thesagro: |
Conservação; Nim; Planta Medicinal. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 02468naa a2200253 a 4500
001 2160010
005 2023-12-29
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.37349/eff.2023.00011$2DOI
100 1 $aSANTOS, K. S.
245 $aAzadirachta indica A. Juss (neem) phenolic extract inhibits human B-lymphoblastoid cells growth via cell cycle arrest, apoptosis induction, and DNA damage.$h[electronic resource]
260 $c2023
520 $aAim: As far as is known, the pharmaceutical effects of neem on human B-lymphoblastoid (TK6) cells have not been studied until now. Hence, the present study aimed to obtain neem phenolic extracts for inhibits the proliferation of TK6 cells and explore some possible underlying mechanisms involved in these effects. Methods: Hexane extract (HE) was obtained in the first step. After that, the residual hexane was removed from the neem. The dried neem sample was used in a new extraction for obtaining the ethyl acetate extract (EAE). Total phenolic compounds (TPC) and total flavonoid contents (TFC) were determined by spectrophotometric methods. Lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) tests were used to evaluate the cytotoxicity in TK6 cells. The stop at G0/G1 cell cycle and inducing apoptosis in the TK6 cells were analyzed by flow cytometry. For deoxyribonucleic acid (DNA) damage evaluation, the alkaline comet test was used. Results: The higher TFC (65.50 mg/g of extract ± 1.17 mg/g of extract) and TPC (52.08 mg of extract ± 0.88 mg of extract) were obtained in EAE compared to HE that was obtained TFC of 14.61 mg/g of extract ± 0.60 mg/g of extract and TPC of 3.20 mg/g of extract ± 1.20 mg/g of extract. EAE was more significantly cytotoxic to TK6 cells than HE. The apoptosis induction was higher after exposure to 15.0 μg/mL of EAE (11.29%) in comparison to 15.0 μg/mL of HE (2.52%). The G0/G1 phase increased from 72% negative control (NC) to 83% after treatment with neem extracts (15 μg/mL). Neem extracts were also able to cause DNA strand breaks in TK6 cells.
650 $aConservação
650 $aNim
650 $aPlanta Medicinal
700 1 $aCOSTA, C.
700 1 $aBESSA, M. J.
700 1 $aTEIXEIRA, J. P.
700 1 $aSILVA, A. V. C. da
700 1 $aPADILHA, F. F.
700 1 $aDARIVA, C.
700 1 $aOLIVEIRA, M. B. P. P.
773 $tExploration of Foods and Foodomics$gv. 1, p. 130-142, 2023.
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Registro original: |
Embrapa Tabuleiros Costeiros (CPATC) |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
11/03/2013 |
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Data da última atualização: |
11/03/2013 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA, S. L.; GOMES, R. da C.; BONIN, M. N.; MARTELLO, L. S.; ALVAREZ, P. L.; OLIVEIRA, L. S.; MAZON, M. R.; NOGUEIRA FILHO, J. C. M. |
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Afiliação: |
S. L. SILVA, USP; RODRIGO DA COSTA GOMES, CNPGC; M. N. BONIN, USP; L. S. MARTELLO, USP; P. L. ALVAREZ, USP; L. S. OLIVEIRA, USP; M. R. MAZON, USP; J. C. M. NOGUEIRA FILHO, USP. |
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Título: |
Feedlot performance and carcass traits of Nellore cattle as affected by sex condition and frame size. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Animal Science v.90, (Supplement 3/J); Journal of Dairy Science, v.95, (Supplement 2), p.99, 2012. |
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Idioma: |
Inglês |
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Notas: |
Edição dos trabalhos do:ADSA-PSA-AMPA-ASAS-CSAS-WSASAS JOINT ANNUAL MEETING, 2012, Phoenix-AR. Ruminant Nutrition: Beef I. M266. |
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Conteúdo: |
Use of non-castrated (NC) males for red meat production has been a common practice in Brazil because they grow fast, utilize feed more efficiently and show high-yielding and leaner carcasses when compared with castrated males (CM). The objective of this work was to investigate the effects of sex condition and frame size (FS) on feedlot performance and carcass traits of finishing Bos indicus steers and bulls. |
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Palavras-Chave: |
Bovino de corte. |
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Thesagro: |
Nutrição Animal. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/78698/1/Rodrigo2.pdf
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Marc: |
LEADER 01285nam a2200229 a 4500
001 1952638
005 2013-03-11
008 2012 bl uuuu u00u1 u #d
100 1 $aSILVA, S. L.
245 $aFeedlot performance and carcass traits of Nellore cattle as affected by sex condition and frame size.$h[electronic resource]
260 $aJournal of Animal Science v.90, (Supplement 3/J); Journal of Dairy Science, v.95, (Supplement 2), p.99$c2012
500 $aEdição dos trabalhos do:ADSA-PSA-AMPA-ASAS-CSAS-WSASAS JOINT ANNUAL MEETING, 2012, Phoenix-AR. Ruminant Nutrition: Beef I. M266.
520 $aUse of non-castrated (NC) males for red meat production has been a common practice in Brazil because they grow fast, utilize feed more efficiently and show high-yielding and leaner carcasses when compared with castrated males (CM). The objective of this work was to investigate the effects of sex condition and frame size (FS) on feedlot performance and carcass traits of finishing Bos indicus steers and bulls.
650 $aNutrição Animal
653 $aBovino de corte
700 1 $aGOMES, R. da C.
700 1 $aBONIN, M. N.
700 1 $aMARTELLO, L. S.
700 1 $aALVAREZ, P. L.
700 1 $aOLIVEIRA, L. S.
700 1 $aMAZON, M. R.
700 1 $aNOGUEIRA FILHO, J. C. M.
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