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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
30/09/2021 |
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Data da última atualização: |
30/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, V. N.; GAMA, R. N. C. S.; SANTOS, C. A. F. |
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Afiliação: |
V. N. SANTOS, Universidade Estadual de Feira de Santana; R. N. C. S. C. GAMA, Universidade Estadual de Feira de Santana; CARLOS ANTONIO FERNANDES SANTOS, CPATSA. |
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Título: |
Development and transferability of microsatellite loci for Spondias tuberosa (Anacardiaceae: Sapindales), a species endemic to the Brazilian semi-arid region. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Research Society and Development, v. 20, n. 2, May. 2021. |
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Idioma: |
Inglês |
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Conteúdo: |
The umbu tree (Spondias tuberosa) is one of the best known plants of the Brazilian semi-arid region; it has great potential for the fruit market due to excellent consumer acceptance. This tree is not presently cultivated; fruit commercialization is based on extrativism. Consequently, there has been little research on the genetics of this species. Our objective was to develop, evaluate and transfer single sequence repeat (SSR) loci to S. tuberosa to support work on genetic resources and agronomic improvement of this species. SSR loci for the umbu tree were developed from a new enriched genomic library and evaluated by PCR. Fourteen SSR loci developed for S. radlkoferi were evaluated for use in S. tuberosa, as well as 18 SSR loci previously identified for this species. DNA was extracted from leaf tissue of eight umbu trees available that are part of a germplasm collection located in Petrolina, PE, Brazil. Of the 14 pairs of primers that were tested, six yielded amplicons, and two showed polymorphism in the genotyped samples. All SSR loci of S. radlkoferi transferred to the umbu tree species, yielding amplicons; however, only four were polymorphic in this sample. Among the eighteen available species-specific SSR loci, six were polymorphic for these eight trees. Among the 38 loci that we evaluated, 12 were polymorphic in the sample, including two new loci for S. tuberosa. The number of alleles ranged from 2 to 12, and 10 of them were moderately to highly informative (PIC>0.50), while nine had heterozygosity greater than 0.50. The six new SSR loci and the 14 SSR loci transferred from S. radlkoferi increase the number of available loci for population studies, germplasm collection and resource management for the development of new umbu tree cultivars. MenosThe umbu tree (Spondias tuberosa) is one of the best known plants of the Brazilian semi-arid region; it has great potential for the fruit market due to excellent consumer acceptance. This tree is not presently cultivated; fruit commercialization is based on extrativism. Consequently, there has been little research on the genetics of this species. Our objective was to develop, evaluate and transfer single sequence repeat (SSR) loci to S. tuberosa to support work on genetic resources and agronomic improvement of this species. SSR loci for the umbu tree were developed from a new enriched genomic library and evaluated by PCR. Fourteen SSR loci developed for S. radlkoferi were evaluated for use in S. tuberosa, as well as 18 SSR loci previously identified for this species. DNA was extracted from leaf tissue of eight umbu trees available that are part of a germplasm collection located in Petrolina, PE, Brazil. Of the 14 pairs of primers that were tested, six yielded amplicons, and two showed polymorphism in the genotyped samples. All SSR loci of S. radlkoferi transferred to the umbu tree species, yielding amplicons; however, only four were polymorphic in this sample. Among the eighteen available species-specific SSR loci, six were polymorphic for these eight trees. Among the 38 loci that we evaluated, 12 were polymorphic in the sample, including two new loci for S. tuberosa. The number of alleles ranged from 2 to 12, and 10 of them were moderately to highly informative (PIC>0.50), ... Mostrar Tudo |
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Palavras-Chave: |
SSR. |
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Thesagro: |
Melhoramento Genético Vegetal; Spondias Tuberosa; Umbu. |
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Thesaurus Nal: |
Plant breeding; Spondias. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/226520/1/Development-and-transferability-of-microsatellite-2021.pdf
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Marc: |
LEADER 02483naa a2200217 a 4500
001 2134937
005 2021-09-30
008 2021 bl uuuu u00u1 u #d
100 1 $aSANTOS, V. N.
245 $aDevelopment and transferability of microsatellite loci for Spondias tuberosa (Anacardiaceae$bSapindales), a species endemic to the Brazilian semi-arid region.$h[electronic resource]
260 $c2021
520 $aThe umbu tree (Spondias tuberosa) is one of the best known plants of the Brazilian semi-arid region; it has great potential for the fruit market due to excellent consumer acceptance. This tree is not presently cultivated; fruit commercialization is based on extrativism. Consequently, there has been little research on the genetics of this species. Our objective was to develop, evaluate and transfer single sequence repeat (SSR) loci to S. tuberosa to support work on genetic resources and agronomic improvement of this species. SSR loci for the umbu tree were developed from a new enriched genomic library and evaluated by PCR. Fourteen SSR loci developed for S. radlkoferi were evaluated for use in S. tuberosa, as well as 18 SSR loci previously identified for this species. DNA was extracted from leaf tissue of eight umbu trees available that are part of a germplasm collection located in Petrolina, PE, Brazil. Of the 14 pairs of primers that were tested, six yielded amplicons, and two showed polymorphism in the genotyped samples. All SSR loci of S. radlkoferi transferred to the umbu tree species, yielding amplicons; however, only four were polymorphic in this sample. Among the eighteen available species-specific SSR loci, six were polymorphic for these eight trees. Among the 38 loci that we evaluated, 12 were polymorphic in the sample, including two new loci for S. tuberosa. The number of alleles ranged from 2 to 12, and 10 of them were moderately to highly informative (PIC>0.50), while nine had heterozygosity greater than 0.50. The six new SSR loci and the 14 SSR loci transferred from S. radlkoferi increase the number of available loci for population studies, germplasm collection and resource management for the development of new umbu tree cultivars.
650 $aPlant breeding
650 $aSpondias
650 $aMelhoramento Genético Vegetal
650 $aSpondias Tuberosa
650 $aUmbu
653 $aSSR
700 1 $aGAMA, R. N. C. S.
700 1 $aSANTOS, C. A. F.
773 $tResearch Society and Development$gv. 20, n. 2, May. 2021.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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| Registros recuperados : 7 | |
| 2. |  | SANTOS, V. N. dos; OLIVEIRA, D. R. M. dos S. A importância do escritório de projetos de pesquisa para gestão de processos. In: MOSTRA DE ESTAGIÁRIOS E BOLSISTAS DA EMBRAPA INFORMÁTICA AGROPCUÁRIA, 13., 2017, Campinas. Resumos expandidos... Brasília, DF: Embrapa, 2017. p. 29-33. Editores técnicos: Giampaolo Queiroz Pellegrino, Luciana Guilherme Sacomani Zenerato, Maria Fernanda Moura, Giulia Croce, Poliana Fernanda Giachetto.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| 6. | | SILVA, A. P. A. DA; SANTOS, V. N.; GALDINO, I. K. C. P. DE O.; LIMA, Z. N.; SANTOS, K. M. O. dos; PEREIRA, E. V. DOS S.; DANTAS, G. C.; BURITI, F. C. A. Atividade antimicrobiana de bactéria lática nativa (Lactiplantibacillus plantarum CNPC001) Potencialmente probiótica frente a Salmonella enterica serovar Typhimurium ATCC 14028. In: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS E NUTRIÇÃO, 15., 2023, Campinas. A revolução da ciência de alimentos e nutrição: alimentando o mundo de forma sustentável: caderno [eletrônico] de resumos. Campinas: Galoá, 2023. SLACAN. 168903 Pôster.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 7. | | GALDINO, I. K. C. P. DE O.; SILVA, M. O. M. DA; SILVA, A. P. A. DA; SANTOS, V. N.; FEITOSA, R. L. P.; FERREIRA, L. C. N.; DANTAS, G. C.; PEREIRA, E. V. DOS S.; OLIVEIRA, T. A. DE; SANTOS, K. M. O. dos; EGITO, A. S. do; BURITI, F. C. A.; CARDARELLI, H. R. β-Glucosidase activity and antimicrobial properties of potentially probiotic autochthonous lactic cultures Beta-Glucosidase activity and antimicrobial properties of potentially probiotic autochthonous lactic cultures. PeerJ, v. 11, e16094, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| Registros recuperados : 7 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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