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Registro Completo |
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
30/12/2021 |
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Data da última atualização: |
01/01/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, J. A. de; SANTOS, M. R. A. dos. |
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Afiliação: |
JAQUELINE ANDRUCHEVITZ DE SOUZA, FIMCA; MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO. |
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Título: |
In vitro propagation of Piper hispidum by somatic embryogenesis in leaf explants. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
International Journal of Development Research, v. 11, n. 12, p. 52392-52393, Dec. 2021. |
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ISSN: |
2230-9926 |
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DOI: |
https://doi.org/10.37118/ijdr.23567.12.2021 |
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Idioma: |
Inglês |
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Conteúdo: |
A protocol for regeneration of P. hispidum through somatic embryogenesis in leaf explants is described. Under aseptic conditions, the leaves were cut into 1.0 cm2 explants. These explants were inoculated into test tubes with 10.0 mL of an Murashige and Skoog (MS) basal culture medium supplemented with 30.0 g L-1 sucrose, 6.0 g L-1 agar and a factorial combination of the growth regulators 2,4-Dichlorophenoxyacetic acid (2,4-D) and 6-Benzylaminopurine (BA), both at 0.0, 1.0, 2.0, 3.0 and 4.0 mg L-1 , totalizing 25 treatments. The pH was adjusted to 5.8 and the medium autoclaved at 121°C for 20 minutes. After 75 days, the average number of cotyledonary somatic embryos in each explant was recorded.Maximum number of 9.2 cotyledonary embryos per explant was observed with the combination of 2.0 mg L-1 BA + 2.0 mg L-1 2,4-D. The subculture of the cotyledonary embryos to a medium without growth regulators resulted in 95% conversion into plantlets, which were acclimatized with 100% survival. |
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Palavras-Chave: |
Cotyledonary embryos; Embriogênese somática; Protocolo de regeneração. |
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Thesagro: |
Cultura de Tecido; Cultura In Vitro; Explante; Folha; Propagação Vegetativa; Reprodução Vegetal. |
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Thesaurus Nal: |
Explants; Genetic techniques and protocols; In vitro culture; Leaves; Piper hispidum; Plant reproduction; Somatic embryogenesis; Tissue culture; Vegetative propagation. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/229831/1/cpafro-18666.pdf
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Marc: |
LEADER 02157naa a2200373 a 4500
001 2138599
005 2022-01-01
008 2021 bl uuuu u00u1 u #d
022 $a2230-9926
024 7 $ahttps://doi.org/10.37118/ijdr.23567.12.2021$2DOI
100 1 $aSOUZA, J. A. de
245 $aIn vitro propagation of Piper hispidum by somatic embryogenesis in leaf explants.$h[electronic resource]
260 $c2021
520 $aA protocol for regeneration of P. hispidum through somatic embryogenesis in leaf explants is described. Under aseptic conditions, the leaves were cut into 1.0 cm2 explants. These explants were inoculated into test tubes with 10.0 mL of an Murashige and Skoog (MS) basal culture medium supplemented with 30.0 g L-1 sucrose, 6.0 g L-1 agar and a factorial combination of the growth regulators 2,4-Dichlorophenoxyacetic acid (2,4-D) and 6-Benzylaminopurine (BA), both at 0.0, 1.0, 2.0, 3.0 and 4.0 mg L-1 , totalizing 25 treatments. The pH was adjusted to 5.8 and the medium autoclaved at 121°C for 20 minutes. After 75 days, the average number of cotyledonary somatic embryos in each explant was recorded.Maximum number of 9.2 cotyledonary embryos per explant was observed with the combination of 2.0 mg L-1 BA + 2.0 mg L-1 2,4-D. The subculture of the cotyledonary embryos to a medium without growth regulators resulted in 95% conversion into plantlets, which were acclimatized with 100% survival.
650 $aExplants
650 $aGenetic techniques and protocols
650 $aIn vitro culture
650 $aLeaves
650 $aPiper hispidum
650 $aPlant reproduction
650 $aSomatic embryogenesis
650 $aTissue culture
650 $aVegetative propagation
650 $aCultura de Tecido
650 $aCultura In Vitro
650 $aExplante
650 $aFolha
650 $aPropagação Vegetativa
650 $aReprodução Vegetal
653 $aCotyledonary embryos
653 $aEmbriogênese somática
653 $aProtocolo de regeneração
700 1 $aSANTOS, M. R. A. dos
773 $tInternational Journal of Development Research$gv. 11, n. 12, p. 52392-52393, Dec. 2021.
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Registro original: |
Embrapa Rondônia (CPAF-RO) |
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| Registros recuperados : 188 | |
| 1. |  | SANTOS, M. R. A. dos. Cultura de tecidos vegetais em Rondônia. Agrosoft, 2008. Meta 2008.
Artigo também publicado com o título: Micropropagação vegetal. Demais veículos em que o artigo foi publicado: Maxpress Net; Cultivar; Portal do Agronegócio; CREA-RO; O Guaporé; Boletim Pecuário; ClicNews; BragaRural;...| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Rondônia. |
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| Registros recuperados : 188 | |
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