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Registro Completo |
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
26/11/2020 |
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Data da última atualização: |
26/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, M. R. A. dos; PAZ, E. S. |
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Afiliação: |
MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO; Universidade Federal de Rondonia. |
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Título: |
Dedifferentation of leaf cells and growth pattern of caluses of Capsicum frutescens cv. Stromboli. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
International Journal of Development Research, v. 10, n. 11, p. 41853-41856, Nov. 2020. |
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ISSN: |
2230-9926 |
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DOI: |
10.37118/ijdr.20362.11.2020 |
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Idioma: |
Inglês |
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Conteúdo: |
In vitro cell suspension cultivation systems have been largely reported as safe and standardized methods of production of secondary metabolites with medicinal and agricultural interest. Capsicum frutescensis one of the most widely grown vegetable in the world and its biological activities have been demonstrated against insects, fungi, bacteria and other groups of organisms. The objective of this study was to establish a protocol for dedifferentiation of leaf cells of the cultivar C. frutescens cv. Stromboli and to determine the growth pattern of the calluses with a focus on the deceleration phase, when the callus cells must be subcultured into a liquid medium in order to establish cell suspension cultivations aiming at the production of secondary metabolites. The explants were inoculated into a medium supplemented with BA and 2,4-D in factorial combinations. The percentage of callus induction (%CI), the explant area covered by callus cells (ACCC) and the fresh weight of the calluses were evaluated. The procedures that resulted in higher proliferation of callus cells were repeated in order to determine the growth curve of the calluses. The treatment that resulted in the highest %CI, ACCC and callus weight was 1.5 mg L-1 2,4-D. The calluses produced were friable and whitish and their growth pattern followed a sigmoid shape. The deceleration phase started on the 39th day of cultivation. |
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Palavras-Chave: |
Amazônia Ocidental; Callogenesis; Capsicum frutescens cv Stromboli; Célula foliar; Deceleration phase; Embrapa Rondônia; Fase de desaceleração; Growth curves; Leaf cells; Metabólicos secundários; Porto Velho (RO); Protocolo de diferenciação celular; Rondônia; Western Amazon. |
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Thesagro: |
Calogênese; Cultura In Vitro; Curva de Crescimento. |
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Thesaurus Nal: |
Callus formation; Cell dedifferentiation; In vitro culture; Secondary metabolites. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/218277/1/cpafro-18474.pdf
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Marc: |
LEADER 02684naa a2200409 a 4500
001 2127111
005 2020-11-26
008 2020 bl uuuu u00u1 u #d
022 $a2230-9926
024 7 $a10.37118/ijdr.20362.11.2020$2DOI
100 1 $aSANTOS, M. R. A. dos
245 $aDedifferentation of leaf cells and growth pattern of caluses of Capsicum frutescens cv. Stromboli.$h[electronic resource]
260 $c2020
520 $aIn vitro cell suspension cultivation systems have been largely reported as safe and standardized methods of production of secondary metabolites with medicinal and agricultural interest. Capsicum frutescensis one of the most widely grown vegetable in the world and its biological activities have been demonstrated against insects, fungi, bacteria and other groups of organisms. The objective of this study was to establish a protocol for dedifferentiation of leaf cells of the cultivar C. frutescens cv. Stromboli and to determine the growth pattern of the calluses with a focus on the deceleration phase, when the callus cells must be subcultured into a liquid medium in order to establish cell suspension cultivations aiming at the production of secondary metabolites. The explants were inoculated into a medium supplemented with BA and 2,4-D in factorial combinations. The percentage of callus induction (%CI), the explant area covered by callus cells (ACCC) and the fresh weight of the calluses were evaluated. The procedures that resulted in higher proliferation of callus cells were repeated in order to determine the growth curve of the calluses. The treatment that resulted in the highest %CI, ACCC and callus weight was 1.5 mg L-1 2,4-D. The calluses produced were friable and whitish and their growth pattern followed a sigmoid shape. The deceleration phase started on the 39th day of cultivation.
650 $aCallus formation
650 $aCell dedifferentiation
650 $aIn vitro culture
650 $aSecondary metabolites
650 $aCalogênese
650 $aCultura In Vitro
650 $aCurva de Crescimento
653 $aAmazônia Ocidental
653 $aCallogenesis
653 $aCapsicum frutescens cv Stromboli
653 $aCélula foliar
653 $aDeceleration phase
653 $aEmbrapa Rondônia
653 $aFase de desaceleração
653 $aGrowth curves
653 $aLeaf cells
653 $aMetabólicos secundários
653 $aPorto Velho (RO)
653 $aProtocolo de diferenciação celular
653 $aRondônia
653 $aWestern Amazon
700 1 $aPAZ, E. S.
773 $tInternational Journal of Development Research$gv. 10, n. 11, p. 41853-41856, Nov. 2020.
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Registro original: |
Embrapa Rondônia (CPAF-RO) |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
04/11/2019 |
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Data da última atualização: |
04/11/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ÂNGELO, F. F.; SCHWAAB, R. D. C.; REZENDE, A. P. M.; LOPES, M. F.; AGUIAR, D. M.; SOUZA, V. de; MELO JUNIOR, A. M.; FERREIRA, M. H. |
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Afiliação: |
Universidade Federal de Juiz de Fora (UFJF) - Juiz de Fora, MG, Brazil; Universidade Federal de Juiz de Fora (UFJF) - Juiz de Fora, MG, Brazil; Universidade Federal de Juiz de Fora (UFJF) - Juiz de Fora, MG, Brazil; Universidade Federal de Juiz de Fora (UFJF) - Juiz de Fora, MG, Brazil; Universidade Federal de Juiz de Fora (UFJF) - Juiz de Fora, MG, Brazil; VIVIANE DE SOUZA, CNPC; Universidade Federal de Juiz de Fora (UFJF) - Juiz de Fora, MG, Brazil; Universidade Federal de Juiz de Fora (UFJF) - Juiz de Fora, MG, Brazil. |
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Título: |
Antimicrobial resistance profile of Staphylococcus aureus isolated in goat milk. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 30., 2019, Maceió, Anais... Maceió: SBM, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The present work aimed to study the antimicrobial resistance profile of Staphylococcus aureus isolated from goal milk. Two hundred and twelve samples of goat milk were used for the microbiological analyses. In ten (4.7%) of these samples, strains of Staphylococcus aureus were isolated. The susceptibility of the strains of S. aureus was assayed by the disc diffusion method. The antibiotics tested were: Ampicillin-10?g (AMP), Chloramphenicol-30?g (CHL), Tetracycline-30?g (TET), Gentamicin-10?g (GEN), Trimethoprim-Sulfamethoxazole-25?g (SXT), Erythromycin-30?g (ERY), Cefoxitin-30?g (CFO), Ciprofloxacin-05?g (CIP), Penicillin-10? (PEN)), Clindamycin-02?g (CLI), Azithromycin-15?g (AZM), Rifampicin-05?g (RIF) and Linezolid 30?g (LZD). The resistance of some strains to the antibiotics tested becomes a concern, because of the difficulty in treating the animal, but also because of a loss of product quality and by virtue of the possibility of contamination in human consumption, which can occur, including, during periods of treatment, when the milk should not be used. |
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Palavras-Chave: |
Antimicrobial resistance; Antimicrobial sensitivity; Antimicrobiano; Resistance to chemicals. |
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Thesagro: |
Bactéria; Cabra Leiteira; Caprino; Doença Animal; Mamite. |
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Thesaurus NAL: |
Bacterial infections; Dairy goats; Mastitis. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/204203/1/CNPC-2019-Antimicrobial.pdf
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Marc: |
LEADER 02082nam a2200337 a 4500
001 2113856
005 2019-11-04
008 2019 bl uuuu u00u1 u #d
100 1 $aÂNGELO, F. F.
245 $aAntimicrobial resistance profile of Staphylococcus aureus isolated in goat milk.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 30., 2019, Maceió, Anais... Maceió: SBM$c2019
520 $aAbstract: The present work aimed to study the antimicrobial resistance profile of Staphylococcus aureus isolated from goal milk. Two hundred and twelve samples of goat milk were used for the microbiological analyses. In ten (4.7%) of these samples, strains of Staphylococcus aureus were isolated. The susceptibility of the strains of S. aureus was assayed by the disc diffusion method. The antibiotics tested were: Ampicillin-10?g (AMP), Chloramphenicol-30?g (CHL), Tetracycline-30?g (TET), Gentamicin-10?g (GEN), Trimethoprim-Sulfamethoxazole-25?g (SXT), Erythromycin-30?g (ERY), Cefoxitin-30?g (CFO), Ciprofloxacin-05?g (CIP), Penicillin-10? (PEN)), Clindamycin-02?g (CLI), Azithromycin-15?g (AZM), Rifampicin-05?g (RIF) and Linezolid 30?g (LZD). The resistance of some strains to the antibiotics tested becomes a concern, because of the difficulty in treating the animal, but also because of a loss of product quality and by virtue of the possibility of contamination in human consumption, which can occur, including, during periods of treatment, when the milk should not be used.
650 $aBacterial infections
650 $aDairy goats
650 $aMastitis
650 $aBactéria
650 $aCabra Leiteira
650 $aCaprino
650 $aDoença Animal
650 $aMamite
653 $aAntimicrobial resistance
653 $aAntimicrobial sensitivity
653 $aAntimicrobiano
653 $aResistance to chemicals
700 1 $aSCHWAAB, R. D. C.
700 1 $aREZENDE, A. P. M.
700 1 $aLOPES, M. F.
700 1 $aAGUIAR, D. M.
700 1 $aSOUZA, V. de
700 1 $aMELO JUNIOR, A. M.
700 1 $aFERREIRA, M. H.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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