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Registro Completo |
Biblioteca(s): |
Embrapa Rondônia. |
Data corrente: |
30/12/2021 |
Data da última atualização: |
01/01/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SOUZA, J. A. de; SANTOS, M. R. A. dos. |
Afiliação: |
JAQUELINE ANDRUCHEVITZ DE SOUZA, FIMCA; MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO. |
Título: |
Direct organogenesis and in vitro regeneration of plants of Piper marginatum. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
International Journal of Development Research, v. 11, n. 12, p. 52440-52442, Dec. 2021. |
ISSN: |
2230-9926 |
DOI: |
https://doi.org/10.37118/ijdr.23568.12.2021 |
Idioma: |
Inglês |
Conteúdo: |
Piper marginatum, popularly known in Brazil as Caapeba, is a native Amazonian plant of shrubby size, traditionally used for its active principles that act as therapeutic agents in wounds and pathologies. The objective of this work was to establish a protocol for the in vitro propagation of this species from leaf explants. For this, leaves were collected from two-year-old plants kept in a greenhouse at Embrapa Rondônia, in Porto Velho, Brazil. The leaves were subjected to disinfestation in a laminar flow chamber by immersion in 70% alcohol for one minute, followed by immersion in 2.0% sodium hypochlorite with 1.0 mL of Tween 20® for five minutes. Leaves were cut into 1.0 cm2 explants, which were inoculated into MS medium supplemented with 30.0 g L-1 sucrose, 6.0 g L-1 agar and variable concentrations of growth regulators benzylaminopurine (BA) (0.0, 1.13, 2.25 and 4.50 mg L-1) and 2,4-dichlorophenoxyacetic acid (2,4-D) (0.0, 1.11, 2.21 and 4.42 mg L-1) in factorial combination. The cultures were kept in a growth room at 25°C, under a 16-hour photoperiod. After 30 days, the number of shoots formed in the explants was evaluated. These shoots were subcultured on MS medium, under the conditions described, but without growth regulators. After 60 days, plantlet height, number of leaves, leaf area and root volume were evaluated. The treatment that contained 1.13 mg L-1 of BAP, in the absence of 2,4-D, resulted in the highest number of shoots and more vigorous plantlets after subculture, in relation to all evaluated variables. The plantlets were acclimatized in a greenhouse, with 50% shading and sprinkler irrigation three times a day, for their conversion into plants. MenosPiper marginatum, popularly known in Brazil as Caapeba, is a native Amazonian plant of shrubby size, traditionally used for its active principles that act as therapeutic agents in wounds and pathologies. The objective of this work was to establish a protocol for the in vitro propagation of this species from leaf explants. For this, leaves were collected from two-year-old plants kept in a greenhouse at Embrapa Rondônia, in Porto Velho, Brazil. The leaves were subjected to disinfestation in a laminar flow chamber by immersion in 70% alcohol for one minute, followed by immersion in 2.0% sodium hypochlorite with 1.0 mL of Tween 20® for five minutes. Leaves were cut into 1.0 cm2 explants, which were inoculated into MS medium supplemented with 30.0 g L-1 sucrose, 6.0 g L-1 agar and variable concentrations of growth regulators benzylaminopurine (BA) (0.0, 1.13, 2.25 and 4.50 mg L-1) and 2,4-dichlorophenoxyacetic acid (2,4-D) (0.0, 1.11, 2.21 and 4.42 mg L-1) in factorial combination. The cultures were kept in a growth room at 25°C, under a 16-hour photoperiod. After 30 days, the number of shoots formed in the explants was evaluated. These shoots were subcultured on MS medium, under the conditions described, but without growth regulators. After 60 days, plantlet height, number of leaves, leaf area and root volume were evaluated. The treatment that contained 1.13 mg L-1 of BAP, in the absence of 2,4-D, resulted in the highest number of shoots and more vigorous plantlets after subcult... Mostrar Tudo |
Palavras-Chave: |
Piper marginatum; Protocolo de propagação. |
Thesagro: |
Caapeba; Cultura In Vitro; Explante; Folha; Organogénese; Planta Medicinal; Propagação Vegetativa; Regeneração; Reprodução Vegetal. |
Thesaurus Nal: |
Explants; Genetic techniques and protocols; In vitro regeneration; Leaves; Medicinal plants; Organogenesis; Plant reproduction; Vegetative propagation. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/229832/1/cpafro-18667.pdf
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Marc: |
LEADER 02852naa a2200385 a 4500 001 2138600 005 2022-01-01 008 2021 bl uuuu u00u1 u #d 022 $a2230-9926 024 7 $ahttps://doi.org/10.37118/ijdr.23568.12.2021$2DOI 100 1 $aSOUZA, J. A. de 245 $aDirect organogenesis and in vitro regeneration of plants of Piper marginatum.$h[electronic resource] 260 $c2021 520 $aPiper marginatum, popularly known in Brazil as Caapeba, is a native Amazonian plant of shrubby size, traditionally used for its active principles that act as therapeutic agents in wounds and pathologies. The objective of this work was to establish a protocol for the in vitro propagation of this species from leaf explants. For this, leaves were collected from two-year-old plants kept in a greenhouse at Embrapa Rondônia, in Porto Velho, Brazil. The leaves were subjected to disinfestation in a laminar flow chamber by immersion in 70% alcohol for one minute, followed by immersion in 2.0% sodium hypochlorite with 1.0 mL of Tween 20® for five minutes. Leaves were cut into 1.0 cm2 explants, which were inoculated into MS medium supplemented with 30.0 g L-1 sucrose, 6.0 g L-1 agar and variable concentrations of growth regulators benzylaminopurine (BA) (0.0, 1.13, 2.25 and 4.50 mg L-1) and 2,4-dichlorophenoxyacetic acid (2,4-D) (0.0, 1.11, 2.21 and 4.42 mg L-1) in factorial combination. The cultures were kept in a growth room at 25°C, under a 16-hour photoperiod. After 30 days, the number of shoots formed in the explants was evaluated. These shoots were subcultured on MS medium, under the conditions described, but without growth regulators. After 60 days, plantlet height, number of leaves, leaf area and root volume were evaluated. The treatment that contained 1.13 mg L-1 of BAP, in the absence of 2,4-D, resulted in the highest number of shoots and more vigorous plantlets after subculture, in relation to all evaluated variables. The plantlets were acclimatized in a greenhouse, with 50% shading and sprinkler irrigation three times a day, for their conversion into plants. 650 $aExplants 650 $aGenetic techniques and protocols 650 $aIn vitro regeneration 650 $aLeaves 650 $aMedicinal plants 650 $aOrganogenesis 650 $aPlant reproduction 650 $aVegetative propagation 650 $aCaapeba 650 $aCultura In Vitro 650 $aExplante 650 $aFolha 650 $aOrganogénese 650 $aPlanta Medicinal 650 $aPropagação Vegetativa 650 $aRegeneração 650 $aReprodução Vegetal 653 $aPiper marginatum 653 $aProtocolo de propagação 700 1 $aSANTOS, M. R. A. dos 773 $tInternational Journal of Development Research$gv. 11, n. 12, p. 52440-52442, Dec. 2021.
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Embrapa Rondônia (CPAF-RO) |
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1. | | SANTOS, M. R. A. dos. Cultura de tecidos vegetais em Rondônia. Agrosoft, 2008. Meta 2008.
Artigo também publicado com o título: Micropropagação vegetal. Demais veículos em que o artigo foi publicado: Maxpress Net; Cultivar; Portal do Agronegócio; CREA-RO; O Guaporé; Boletim Pecuário; ClicNews; BragaRural;...Tipo: Artigo de Divulgação na Mídia |
Biblioteca(s): Embrapa Rondônia. |
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