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Registro Completo |
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
28/11/2017 |
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Data da última atualização: |
29/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, M. R. A. dos; AGUIAR, C. B.; MENDES, A. M. |
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Afiliação: |
MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-Rondonia. |
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Título: |
Dedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
International Journal of Development Research, v. 7, n. 6, p.13062-13066, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Chili peppers belong to the genus Capsicum and have been studied for their bioactivity, provided by secondary metabolites, which can be used for medicinal and agricultural purposes. Some Capsicum species have been submitted to in vitro cultivation systems of cell suspensions, which have been largely reported as safe and standardized methods of production of secondary compounds. The objective of this study was to determine a protocol for the establishment of cell suspensions from stems of the cultivar C. chinense cv. Airetama. For this, it was necessary to determine the combinations and concentrations of growth regulators for callus induction and to study the growth pattern of the calluses with a focus on the deceleration phase. In this phase the callus cells must be subcultured in a liquid medium in order to establish cell suspension cultivations. Stem explants were inoculated into media supplemented with 2,4-D and BA. The proliferation of callus cells and their growth pattern were evaluated. The highest callus cell proliferation occurred with the combination of 18.10 µM 2,4-D + 2.22 µM BA. The calluses were friable and whitish and their growth pattern followed a sigmoid shape. The deceleration phase started on the 42nd day. |
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Palavras-Chave: |
Callogenesis; Growlh curve. |
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Thesaurus Nal: |
secondary metabolites. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/167695/1/2017-IJDR-Airetama.pdf
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Marc: |
LEADER 01829naa a2200181 a 4500
001 2080950
005 2017-11-29
008 2017 bl uuuu u00u1 u #d
100 1 $aSANTOS, M. R. A. dos
245 $aDedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama.$h[electronic resource]
260 $c2017
520 $aChili peppers belong to the genus Capsicum and have been studied for their bioactivity, provided by secondary metabolites, which can be used for medicinal and agricultural purposes. Some Capsicum species have been submitted to in vitro cultivation systems of cell suspensions, which have been largely reported as safe and standardized methods of production of secondary compounds. The objective of this study was to determine a protocol for the establishment of cell suspensions from stems of the cultivar C. chinense cv. Airetama. For this, it was necessary to determine the combinations and concentrations of growth regulators for callus induction and to study the growth pattern of the calluses with a focus on the deceleration phase. In this phase the callus cells must be subcultured in a liquid medium in order to establish cell suspension cultivations. Stem explants were inoculated into media supplemented with 2,4-D and BA. The proliferation of callus cells and their growth pattern were evaluated. The highest callus cell proliferation occurred with the combination of 18.10 µM 2,4-D + 2.22 µM BA. The calluses were friable and whitish and their growth pattern followed a sigmoid shape. The deceleration phase started on the 42nd day.
650 $asecondary metabolites
653 $aCallogenesis
653 $aGrowlh curve
700 1 $aAGUIAR, C. B.
700 1 $aMENDES, A. M.
773 $tInternational Journal of Development Research$gv. 7, n. 6, p.13062-13066, 2017.
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