Data/hora: 10/06/2024 04:31:25

Biblioteca(s): | Embrapa Rondônia.
Data corrente: | 28/11/2017
Data da última atualização: | 29/11/2017
Autoria: | SANTOS, M. R. A. dos; AGUIAR, C. B.; MENDES, A. M.
Afiliação: | MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-Rondonia.
Título: | Dedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama.
Ano de publicação: | 2017
Fonte/Imprenta: | International Journal of Development Research, v. 7, n. 6, p.13062-13066, 2017.
Idioma: | Inglês
Conteúdo: | Chili peppers belong to the genus Capsicum and have been studied for their bioactivity, provided by secondary metabolites, which can be used for medicinal and agricultural purposes. Some Capsicum species have been submitted to in vitro cultivation systems of cell suspensions, which have been largely reported as safe and standardized methods of production of secondary compounds. The objective of this study was to determine a protocol for the establishment of cell suspensions from stems of the cultivar C. chinense cv. Airetama. For this, it was necessary to determine the combinations and concentrations of growth regulators for callus induction and to study the growth pattern of the calluses with a focus on the deceleration phase. In this phase the callus cells must be subcultured in a liquid medium in order to establish cell suspension cultivations. Stem explants were inoculated into media supplemented with 2,4-D and BA. The proliferation of callus cells and their growth pattern were evaluated. The highest callus cell proliferation occurred with the combination of 18.10 µM 2,4-D + 2.22 µM BA. The calluses were friable and whitish and their growth pattern followed a sigmoid shape. The deceleration phase started on the 42nd day.
Palavras-Chave: | Callogenesis; Growlh curve.
Thesaurus Nal: | secondary metabolites.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/167695/1/2017-IJDR-Airetama.pdf
Registro original: | Embrapa Rondônia (CPAF-RO)
Exemplares: | CPAF-RO - (17943-1) - UPC - AP - DD - SP-3975 -  - SP-3975 - DIS