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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
04/07/2019 |
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Data da última atualização: |
04/07/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAITANO-BERTOLACCI, M. A. B.; BASTOS, R. R. P.; SANTOS, L. R. dos; SOARES, C. O.; RIEGER, J. S. G.; MANTOVANI, C.; SANTANA, M. de S.; ROSINHA, G. M. S. |
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Afiliação: |
Universidade Federal de Mato Grosso do Sul/FUFMS/Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição; Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina Veterinária e Zootecnia; LENITA RAMIRES DOS SANTOS, CNPGC; CLEBER OLIVEIRA SOARES, CNPGC; Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição; Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição; CNPGC; GRACIA MARIA SOARES ROSINHA, CNPGC. |
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Título: |
DNA vaccine and DNA prime-protein boost with the virB9 and virB12 genes induced low level of protection against Brucella abortus infection in mice. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 18, n. 2, gmr18295, 2019 |
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Idioma: |
Inglês |
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Conteúdo: |
VIRB proteins from Brucella spp. constitute the type IV Secretion System (T4SS), a key virulence factor that mediates the intracellular survival of these bacteria. We investigated the immunogenicity and protection of proteins produced by the virB9 and virB12 genes in the DNA vaccine and DNA prime-protein boost strategies. Groups of 10 mice were vaccinated with pcDNAvirB9, pcDNAvirB12, pcDNAvirB9+rVIRB9 or pcDNAvirB12+rVIRB12. The latter two groups were vaccinated with the proteins rVIRB9 and rVIRB12, respectively, during the third immunization. Three weeks after the last immunization, six animals from each group were challenged intraperitoneally with B. abortus strain S2308, and the efficacy of the vaccines was calculated as the log10 of protection by subtracting the mean log CFU of the vaccinated group from the mean log CFU of the negative control group (injected with sterile saline). Most of the vaccinated mice produced total IgG and the subclasses IgG1 and IgG2a against the respective protein, except for the mice vaccinated with pcDNAvirB12. Cytokines IFN-γ and IL-10 were produced, but without a significant difference between the vaccinated and negative control groups. The vaccines did not induce significant levels of protection, in contrast to the immunization obtained with the S19 vaccine strain (Log10, 1.48). In conclusion, the virB9 and virB12 genes of B. abortus, using DNA vaccine and DNA prime-protein boost strategies, were able to induce both humoral and cellular immune responses, but not enough to induce significant protection in the immunized mice. However, given the response in this system, further investigations using the virB9 and virB12 genes of Brucella spp., together with different immune modulators, are warranted. An effort should be made to direct and enhance the immune response, in order to identify a combination that stimulates a better immune response and, consequently, a better level of protection. MenosVIRB proteins from Brucella spp. constitute the type IV Secretion System (T4SS), a key virulence factor that mediates the intracellular survival of these bacteria. We investigated the immunogenicity and protection of proteins produced by the virB9 and virB12 genes in the DNA vaccine and DNA prime-protein boost strategies. Groups of 10 mice were vaccinated with pcDNAvirB9, pcDNAvirB12, pcDNAvirB9+rVIRB9 or pcDNAvirB12+rVIRB12. The latter two groups were vaccinated with the proteins rVIRB9 and rVIRB12, respectively, during the third immunization. Three weeks after the last immunization, six animals from each group were challenged intraperitoneally with B. abortus strain S2308, and the efficacy of the vaccines was calculated as the log10 of protection by subtracting the mean log CFU of the vaccinated group from the mean log CFU of the negative control group (injected with sterile saline). Most of the vaccinated mice produced total IgG and the subclasses IgG1 and IgG2a against the respective protein, except for the mice vaccinated with pcDNAvirB12. Cytokines IFN-γ and IL-10 were produced, but without a significant difference between the vaccinated and negative control groups. The vaccines did not induce significant levels of protection, in contrast to the immunization obtained with the S19 vaccine strain (Log10, 1.48). In conclusion, the virB9 and virB12 genes of B. abortus, using DNA vaccine and DNA prime-protein boost strategies, were able to induce both humoral and cellu... Mostrar Tudo |
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Palavras-Chave: |
DNA vaccine; T4SS; VirB operon; VirB12; VirB9. |
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Thesagro: |
Brucella Abortus. |
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Thesaurus Nal: |
Bovine brucellosis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02847naa a2200289 a 4500
001 2110376
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100 1 $aCAITANO-BERTOLACCI, M. A. B.
245 $aDNA vaccine and DNA prime-protein boost with the virB9 and virB12 genes induced low level of protection against Brucella abortus infection in mice.$h[electronic resource]
260 $c2019
520 $aVIRB proteins from Brucella spp. constitute the type IV Secretion System (T4SS), a key virulence factor that mediates the intracellular survival of these bacteria. We investigated the immunogenicity and protection of proteins produced by the virB9 and virB12 genes in the DNA vaccine and DNA prime-protein boost strategies. Groups of 10 mice were vaccinated with pcDNAvirB9, pcDNAvirB12, pcDNAvirB9+rVIRB9 or pcDNAvirB12+rVIRB12. The latter two groups were vaccinated with the proteins rVIRB9 and rVIRB12, respectively, during the third immunization. Three weeks after the last immunization, six animals from each group were challenged intraperitoneally with B. abortus strain S2308, and the efficacy of the vaccines was calculated as the log10 of protection by subtracting the mean log CFU of the vaccinated group from the mean log CFU of the negative control group (injected with sterile saline). Most of the vaccinated mice produced total IgG and the subclasses IgG1 and IgG2a against the respective protein, except for the mice vaccinated with pcDNAvirB12. Cytokines IFN-γ and IL-10 were produced, but without a significant difference between the vaccinated and negative control groups. The vaccines did not induce significant levels of protection, in contrast to the immunization obtained with the S19 vaccine strain (Log10, 1.48). In conclusion, the virB9 and virB12 genes of B. abortus, using DNA vaccine and DNA prime-protein boost strategies, were able to induce both humoral and cellular immune responses, but not enough to induce significant protection in the immunized mice. However, given the response in this system, further investigations using the virB9 and virB12 genes of Brucella spp., together with different immune modulators, are warranted. An effort should be made to direct and enhance the immune response, in order to identify a combination that stimulates a better immune response and, consequently, a better level of protection.
650 $aBovine brucellosis
650 $aBrucella Abortus
653 $aDNA vaccine
653 $aT4SS
653 $aVirB operon
653 $aVirB12
653 $aVirB9
700 1 $aBASTOS, R. R. P.
700 1 $aSANTOS, L. R. dos
700 1 $aSOARES, C. O.
700 1 $aRIEGER, J. S. G.
700 1 $aMANTOVANI, C.
700 1 $aSANTANA, M. de S.
700 1 $aROSINHA, G. M. S.
773 $tGenetics and Molecular Research$gv. 18, n. 2, gmr18295, 2019
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Embrapa Gado de Corte (CNPGC) |
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| Registros recuperados : 173 | |
| 1. |  | SANTOS, L. R. P. Cuias ameaçadas. Revista Globo Rural, São Paulo, v. 23, n. 273, p. 31, jul. 2008. Informações prestadas por Marina Castelo Branco e Gilmar Paulo Henz, pesquisadores da Embrapa Hortaliças.| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Hortaliças. |
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| 7. | | SANTOS, L. R. dos; GASPAR, E. B. Vacina sim: na medicina veterinária também. Disponível em: https://www.embrapa.br/busca-de-noticias/-/noticia/64113417/artigo----vacina-sim-na-medicina-veterinaria-tambem. Acesso em: 21 out. 2021. Disponibilizado também em: https://www.agorams.com.br/vacina-sim-na-medicina-veterinaria-tambem/| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 10. | | RIBEIRO, S. M.; ROSINHA, G. M. S.; SANTOS, L. R. dos; SANTOS, G. M. Avaliação da resposta imune de camundongos BALB/c a uma proteína recombinante visando o desenvolvimento de vacina contra tristeza parasitária bovina. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 11., 2015, Campo Grande, MS. Anais... Campo Grande: Embrapa Gado de Corte, 2015. 114 p. (Embrapa Gado de Corte. Documentos, 213).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 16. | | SANTOS, L. R. R. dos; LEMOS, O. F. de; RODRIGUES, S. de M. Germinação in vitro de diferentes cultivares de pimenta-do-reino (Piper nigrum L.). In: SEMINÁRIO CIENTÍFICO DA UFRA, 7.; SEMINÁRIO [DE INICIAÇÃO CIENTÍFICA] DA EMBRAPA AMAZÔNIA ORIENTAL, 13.; SEMINÁRIO DE PESQUISA DA UFRA, 1., 2009, Belém, PA. Pesquisa e desenvolvimento tecnológico na formação do jovem cientista: anais. Belém, PA: UFRA: Embrapa Amazônia Oriental, 2009. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 18. | | CAMPELO, M. F.; LEMOS, O. F. de; SANTOS, L. R. R. dos; MIRANDA, S. de R. Proliferação de brotos de cultivares de bananeira em cinco subcultivos em diferentes concentrações de benzilaminopurina a cada subcultivo. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA ORIENTAL, 15., 2011, Belém, PA. A ciência de fazer ciência: anais. Belém, PA: Embrapa Amazônia Oriental, 2011. 1 CD-ROM. PIBIC-2011.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 19. | | SANTOS, L. R. R. dos; LEMOS, O. F. de; RODRIGUES, S. de M.; MONDIN, M. Quantidade de DNA nuclear de genótipos do gênero Piper. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA, 19.; SEMINÁRIO DE PÓS-GRADUAÇÃO DA EMBRAPA AMAZÔNIA ORIENTAL, 3., 2015, Belém, PA. Anais. Belém, PA: Embrapa Amazônia Oriental, 2015. p. 331-334.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 20. | | SANTOS, L. R. dos; ZANGIROLAMI, M. de S.; SILVA, N. O.; VALDERRAMA, P.; MARÇO, P. H. Rapid non-invasive assessment of quality parameters in ground soybean using near-infrared spectroscopy. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 53, n. 1, p. 97-104, jan. 2018. Título em português: Avaliação rápida não invasiva de parâmetros de qualidade em soja triturada com uso de espectroscopia de infravermelho próximo.| Biblioteca(s): Embrapa Unidades Centrais. |
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| Registros recuperados : 173 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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