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Registro Completo |
Biblioteca(s): |
Embrapa Suínos e Aves; Embrapa Unidades Centrais. |
Data corrente: |
20/12/2017 |
Data da última atualização: |
21/02/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PEREIRA, C. E. R.; VANNUCCI, F. A.; GABARDO, M. de P.; SANTOS, L. F. dos; MORES, N.; GUEDES, R. M. C. |
Afiliação: |
Carlos E. R. Pereira, Departamento de Clínica e Cirurgia/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG; Fabio A. Vannucci, Laboratório de Microbiologia Veterinária; Michelle de P. Gabardo, Departamento de Clínica e Cirurgia/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG; Lucas F. dos Santos; NELSON MORES, CNPSA; Roberto M. C. Guedes, Departamento de Clínica e Cirurgia/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG. |
Título: |
Mycoplasma hyorhinis infection in early cases of mycoplasmal pneumonia in swine and evaluation of diagnostic assays. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n, 10, p. 1057-1063, outubro 2017. |
Idioma: |
Inglês |
Notas: |
Título em português: Infecção por Mycoplasma hyorhinis em casos precoces de pneumonia micoplásmica em suínos e comparação entre técnicas diagnósticas. |
Conteúdo: |
Mycoplasmal pneumonia is an important disease in the pig industry. Due to the controversial role of Mycoplasma hyorhinis in this disease, confirmation of the presence of this bacterium and the identification of its roles in respiratory disease remain major challenges. The objectives of this study were to evaluate the presence of M. hyorhinis in early cases of mycoplasmal pneumonia and to determine the usefulness of fluorescent in situ hybridization (FISH) for the diagnosis of respiratory mycoplasmosis in naturally infected pigs. Ninety M. hyopneumoniae and/or M. hyorhinis-infected lung tissue samples based on diagnostic mosaic (DM) were used. The average age of the animals was 116 and 57 days (P<0.01) for groups 1 (positive-M. hyopneumoniae only) and 2 (positive-M. hyorhinis only), respectively. These findings suggest that development of lesions caused by M. hyorhinis occurs earlier than for M. hyopneumoniae. Using the DM as the gold standard, the sensitivity and specificity of FISH for M. hyopneumoniae were 75 and 100%, respectively, and were 40 and 73.3% for the immunohistochemistry (IHC). The sensitivity and specificity of FISH for M. hyorhinis were 76.7 and 100%, respectively. These findings demonstrate that FISH can be a useful tool for diagnosing mycoplasmosis. Viral antigens (PCV2 or influenza A) were detected in 53.3% (16/30) of the samples in group 2 (M. hyorhinis-PCR positive) and 13.3% (4/30) of the samples in group 1 (M. hyopneumoniae-PCR positive). This finding indicates that the
association of M. hyorhinis and viral infection in nursery pigs likely starts due to a viral immunosuppressive condition. MenosMycoplasmal pneumonia is an important disease in the pig industry. Due to the controversial role of Mycoplasma hyorhinis in this disease, confirmation of the presence of this bacterium and the identification of its roles in respiratory disease remain major challenges. The objectives of this study were to evaluate the presence of M. hyorhinis in early cases of mycoplasmal pneumonia and to determine the usefulness of fluorescent in situ hybridization (FISH) for the diagnosis of respiratory mycoplasmosis in naturally infected pigs. Ninety M. hyopneumoniae and/or M. hyorhinis-infected lung tissue samples based on diagnostic mosaic (DM) were used. The average age of the animals was 116 and 57 days (P<0.01) for groups 1 (positive-M. hyopneumoniae only) and 2 (positive-M. hyorhinis only), respectively. These findings suggest that development of lesions caused by M. hyorhinis occurs earlier than for M. hyopneumoniae. Using the DM as the gold standard, the sensitivity and specificity of FISH for M. hyopneumoniae were 75 and 100%, respectively, and were 40 and 73.3% for the immunohistochemistry (IHC). The sensitivity and specificity of FISH for M. hyorhinis were 76.7 and 100%, respectively. These findings demonstrate that FISH can be a useful tool for diagnosing mycoplasmosis. Viral antigens (PCV2 or influenza A) were detected in 53.3% (16/30) of the samples in group 2 (M. hyorhinis-PCR positive) and 13.3% (4/30) of the samples in group 1 (M. hyopneumoniae-PCR positive). This findin... Mostrar Tudo |
Palavras-Chave: |
Mmunohistochemistry; Mycoplasmal pneumonia; Swines. |
Thesaurus Nal: |
Fluorescence in situ hybridization; Mycoplasma hyopneumoniae; Mycoplasma hyorhinis. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/169437/1/Mycoplasma-hyorhinis-infection.pdf
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Marc: |
LEADER 02663naa a2200265 a 4500 001 2088004 005 2018-02-21 008 2017 bl uuuu u00u1 u #d 100 1 $aPEREIRA, C. E. R. 245 $aMycoplasma hyorhinis infection in early cases of mycoplasmal pneumonia in swine and evaluation of diagnostic assays.$h[electronic resource] 260 $c2017 500 $aTítulo em português: Infecção por Mycoplasma hyorhinis em casos precoces de pneumonia micoplásmica em suínos e comparação entre técnicas diagnósticas. 520 $aMycoplasmal pneumonia is an important disease in the pig industry. Due to the controversial role of Mycoplasma hyorhinis in this disease, confirmation of the presence of this bacterium and the identification of its roles in respiratory disease remain major challenges. The objectives of this study were to evaluate the presence of M. hyorhinis in early cases of mycoplasmal pneumonia and to determine the usefulness of fluorescent in situ hybridization (FISH) for the diagnosis of respiratory mycoplasmosis in naturally infected pigs. Ninety M. hyopneumoniae and/or M. hyorhinis-infected lung tissue samples based on diagnostic mosaic (DM) were used. The average age of the animals was 116 and 57 days (P<0.01) for groups 1 (positive-M. hyopneumoniae only) and 2 (positive-M. hyorhinis only), respectively. These findings suggest that development of lesions caused by M. hyorhinis occurs earlier than for M. hyopneumoniae. Using the DM as the gold standard, the sensitivity and specificity of FISH for M. hyopneumoniae were 75 and 100%, respectively, and were 40 and 73.3% for the immunohistochemistry (IHC). The sensitivity and specificity of FISH for M. hyorhinis were 76.7 and 100%, respectively. These findings demonstrate that FISH can be a useful tool for diagnosing mycoplasmosis. Viral antigens (PCV2 or influenza A) were detected in 53.3% (16/30) of the samples in group 2 (M. hyorhinis-PCR positive) and 13.3% (4/30) of the samples in group 1 (M. hyopneumoniae-PCR positive). This finding indicates that the association of M. hyorhinis and viral infection in nursery pigs likely starts due to a viral immunosuppressive condition. 650 $aFluorescence in situ hybridization 650 $aMycoplasma hyopneumoniae 650 $aMycoplasma hyorhinis 653 $aMmunohistochemistry 653 $aMycoplasmal pneumonia 653 $aSwines 700 1 $aVANNUCCI, F. A. 700 1 $aGABARDO, M. de P. 700 1 $aSANTOS, L. F. dos 700 1 $aMORES, N. 700 1 $aGUEDES, R. M. C. 773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n, 10, p. 1057-1063, outubro 2017.
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Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
Biblioteca(s): |
Embrapa Trigo. |
Data corrente: |
20/10/2015 |
Data da última atualização: |
15/04/2016 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
ALMEIDA, R. P. de; MUTADIUA, C. A. P.; MIRANDA, C. H. B.; FAVARO, S. P.; ROCHA, M. de M.; FERREIRA, G. B.; CRUZ, I.; DENARDIN, J. E.; NEUMAIER, N.; SILVA FILHO, P. M. da; CARVALHO, M. da C. S.; NHANHOMBE, E. |
Afiliação: |
RAUL PORFIRIO DE ALMEIDA, CNPA; CELSO AMÉRICO PEDRO MUTADIUA, MRE-ABC; CESAR HERACLIDES BEHLING MIRANDA, CNPAE; SIMONE PALMA FAVARO, CNPAE; MAURISRAEL DE MOURA ROCHA, CPAMN; GILVAN BARBOSA FERREIRA, CNPA; IVAN CRUZ, CNPMS; JOSE ELOIR DENARDIN, CNPT; NORMAN NEUMAIER, CNPSO; PEDRO MOREIRA DA SILVA FILHO, CNPSO; MARIA DA CONCEICAO SANTANA CARVALHO, CNPAF; ERGINO NHANHOMBE, UNIVERSIDADE PEDAGÓGICA,LICHINGA,MOÇAMBIQUE. |
Título: |
Flutuação populacional de insetos da cultura do feijão-nhemba em Lichinga, Niassa, Moçambique. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
In: SEMINÁRIO DE DIVULGAÇÃO DE RESULTADOS DA INVESTIGAÇÃO AGRÁRIA NO CORREDOR DE NACALA, 2., 2015, Lichinga, Moçambique. Anais... Lichinga, Moçambique: Instituto de Investigação Agrária de Moçambique, 2015. Editado por Fernando João Sualei, Oscar Chichongue, Guilhermino Boina, Simone Palma Favaro, Cesar Heraclides Behling Miranda. p. 247-251. |
Idioma: |
Português |
Palavras-Chave: |
INSETOS. |
Thesagro: |
Feijão. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/131412/1/ID43282-2015SDRIACNacala-p247.pdf
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Marc: |
LEADER 01077nam a2200253 a 4500 001 2026793 005 2016-04-15 008 2015 bl uuuu u00u1 u #d 100 1 $aALMEIDA, R. P. de 245 $aFlutuação populacional de insetos da cultura do feijão-nhemba em Lichinga, Niassa, Moçambique.$h[electronic resource] 260 $aIn: SEMINÁRIO DE DIVULGAÇÃO DE RESULTADOS DA INVESTIGAÇÃO AGRÁRIA NO CORREDOR DE NACALA, 2., 2015, Lichinga, Moçambique. Anais... Lichinga, Moçambique: Instituto de Investigação Agrária de Moçambique, 2015. Editado por Fernando João Sualei, Oscar Chichongue, Guilhermino Boina, Simone Palma Favaro, Cesar Heraclides Behling Miranda. p. 247-251.$c2015 650 $aFeijão 653 $aINSETOS 700 1 $aMUTADIUA, C. A. P. 700 1 $aMIRANDA, C. H. B. 700 1 $aFAVARO, S. P. 700 1 $aROCHA, M. de M. 700 1 $aFERREIRA, G. B. 700 1 $aCRUZ, I. 700 1 $aDENARDIN, J. E. 700 1 $aNEUMAIER, N. 700 1 $aSILVA FILHO, P. M. da 700 1 $aCARVALHO, M. da C. S. 700 1 $aNHANHOMBE, E.
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