Data/hora: 14/06/2024 06:48:31

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 12/07/2023
Data da última atualização: | 13/07/2023
Autoria: | VILA VERDE, D. DOS S.; MENDES, M. I. DE S.; SOUZA, A. da S.; AUD, F. F.; SANTOS, K. C. F. dos; SOARES FILHO, W. dos S.; GESTEIRA, A. da S.; COSTA, M. G. C.
Afiliação: | DENISE DOS SANTOS VILA VERDE, UNIVERSIDADE ESTADUAL DE SANTA CRUZ; MARIA INÊS DE SOUZA MENDES; ANTONIO DA SILVA SOUZA, CNPMF; FABIANA FERRAZ AUD, CNPMF; KAREN CRISTINA FIALHO DOS SANTOS, CNPMF; WALTER DOS SANTOS SOARES FILHO, CNPMF; ABELMON DA SILVA GESTEIRA, CNPMF; MÁRCIO GILBERTO CARDOSO COSTA, UNIVERSIDADE ESTADUAL DE SANTA CRUZ.
Título: | Obtaining spontaneous haploids and polyploids from seeds derived from tangerine-type fruits.
Ano de publicação: | 2023
Fonte/Imprenta: | Plant Cell, Tissue and Organ Culture, january, 2023.
DOI: | https://doi.org/10.1007/s11240-023-02449-y
Idioma: | Inglês
Conteúdo: | Obtaining citrus polyploids, especially from triploid individuals, is of great importance for genetic improvement programs, as they produce seedless fruits. Therefore, the aim of this study was to use flow cytometry analyses to identify polyploid individuals among tangerine-type seedlings originating from seeds germinated in vitro. Fruits were collected from 13 different genotypes in the municipality of Mucugê (state of Bahia, Brazil). The seeds were then removed and classified as developed (seeds with well-formed integument), underdeveloped (seeds with wrinkled integument) and small (seeds of 1/3 to 1/6 of normal size). After the integument was removed, the seeds were disinfected and introduced into a WPM culture medium, in which they remained for 120 days. After this period, the percentage of germinated seeds and growth variables were evaluated, and ploidy analysis was conducted using flow cytometry. The developed seeds displayed a higher germination rate (91.5%) than the others. The small and underdeveloped seeds resulted in a considerable frequency of spontaneous polyploids (11%), and one haploid plant was also obtained from the latter class.
Thesagro: | Fruta Cítrica; Tangerina.
Thesaurus Nal: | Citrus; Flow cytometry; Tetraploidy; Triploidy.
Categoria do assunto: | G Melhoramento Genético
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (33816-1) - UPC - AP - DD - Publicação digital -  -  - DIS