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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
03/08/2020 |
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Data da última atualização: |
03/08/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BRAIR, V. L.; MAIA, A. L. R. S.; CORREIA, L. F. L.; BARBOSA, N. O.; SANTOS, J. D. R.; BRANDÃO, F. Z.; FONSECA, J. F. da; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G. |
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Afiliação: |
VIVIANE L. BRAIR, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; ANA LUCIA R. S. MAIA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; LUCAS FRANCISCO L. CORREIA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; NATHALIA O. BARBOSA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JULIANA D. R. SANTOS, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; FELIPE Z. BRANDÃO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JEFERSON FERREIRA DA FONSECA, CNPC; RIBRIO IVAN T. P. BATISTA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JOANNA M. G. SOUZA-FABJAN, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil. |
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Título: |
Gene expression patterns of in vivo-derived sheep blastocysts is more affected by vitrification than slow freezing technique. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Cryobiology, v. 95, p. 110-115, 2020. |
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DOI: |
https://doi.org/10.1016/j.cryobiol.2020.05.009 |
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Idioma: |
Inglês |
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Conteúdo: |
Transfer of fresh sheep embryos frequently results in higher pregnancy rate compared to cryopreserved ones, possibly due to a failure in the communication between the cryopreserved embryo and the endometrium during pre-implantation and pregnancy establishment. Thus, this study assessed the effect of sheep embryo cryopres-ervation (slow freezing or vitrification) on embryo survival rate and expression of genes related to trophectoderm differentiation (CDX2), pluripotency maintenance (NANOG), cell proliferation (TGFB1), mitochondrial activity (NRF1) and apoptosis (BAX and BCL2). Superovulation (n ¼32 ewes) was performed and embryos were transcervically collected. One hundred good quality (Grade I and II) embryos were allocated into three groups: fresh embryos (CTL; n ¼15), slow freezing (SF; n ¼42) or vitrification (VT; n ¼43). After thawing/warming, three pools of five blastocysts per group were used for RT-qPCR; the remaining 55 embryos were cultured in vitro in SOFaa medium at 38.5 �C and 5% CO2 (SF: n ¼27 and VT: n ¼28). Survival rate of SF and VT were, respectively, 29.6% (8/27) and 14.2% (4/28) at 24 h; and 48.1% (13/27) and 32.1% (9/28) at 48 h (P >0.05). Only CDX2 was affected (up-regulated, P <0.05) in both groups compared to CTL. The BAX transcript was upregulated in VT, compared to SF group. The VT increased (P <0.05) the expression of all genes, except for NANOG and NRF1, when compared to the CTL. In conclusion, although in vitro survival was similar between techniques, VT led to increased changes in blastocyst gene expression compared to CTL and SF. MenosTransfer of fresh sheep embryos frequently results in higher pregnancy rate compared to cryopreserved ones, possibly due to a failure in the communication between the cryopreserved embryo and the endometrium during pre-implantation and pregnancy establishment. Thus, this study assessed the effect of sheep embryo cryopres-ervation (slow freezing or vitrification) on embryo survival rate and expression of genes related to trophectoderm differentiation (CDX2), pluripotency maintenance (NANOG), cell proliferation (TGFB1), mitochondrial activity (NRF1) and apoptosis (BAX and BCL2). Superovulation (n ¼32 ewes) was performed and embryos were transcervically collected. One hundred good quality (Grade I and II) embryos were allocated into three groups: fresh embryos (CTL; n ¼15), slow freezing (SF; n ¼42) or vitrification (VT; n ¼43). After thawing/warming, three pools of five blastocysts per group were used for RT-qPCR; the remaining 55 embryos were cultured in vitro in SOFaa medium at 38.5 �C and 5% CO2 (SF: n ¼27 and VT: n ¼28). Survival rate of SF and VT were, respectively, 29.6% (8/27) and 14.2% (4/28) at 24 h; and 48.1% (13/27) and 32.1% (9/28) at 48 h (P >0.05). Only CDX2 was affected (up-regulated, P <0.05) in both groups compared to CTL. The BAX transcript was upregulated in VT, compared to SF group. The VT increased (P <0.05) the expression of all genes, except for NANOG and NRF1, when compared to the CTL. In conclusion, although in vitro survival was similar between... Mostrar Tudo |
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Palavras-Chave: |
Apoptosis regulator; Embryo cryopreservation; Embryonic metabolism; Reproductive biotechnologies. |
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Thesaurus Nal: |
Embryo implantation; Embryo transfer; Ewes. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02602naa a2200313 a 4500
001 2124160
005 2020-08-03
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.cryobiol.2020.05.009$2DOI
100 1 $aBRAIR, V. L.
245 $aGene expression patterns of in vivo-derived sheep blastocysts is more affected by vitrification than slow freezing technique.$h[electronic resource]
260 $c2020
520 $aTransfer of fresh sheep embryos frequently results in higher pregnancy rate compared to cryopreserved ones, possibly due to a failure in the communication between the cryopreserved embryo and the endometrium during pre-implantation and pregnancy establishment. Thus, this study assessed the effect of sheep embryo cryopres-ervation (slow freezing or vitrification) on embryo survival rate and expression of genes related to trophectoderm differentiation (CDX2), pluripotency maintenance (NANOG), cell proliferation (TGFB1), mitochondrial activity (NRF1) and apoptosis (BAX and BCL2). Superovulation (n ¼32 ewes) was performed and embryos were transcervically collected. One hundred good quality (Grade I and II) embryos were allocated into three groups: fresh embryos (CTL; n ¼15), slow freezing (SF; n ¼42) or vitrification (VT; n ¼43). After thawing/warming, three pools of five blastocysts per group were used for RT-qPCR; the remaining 55 embryos were cultured in vitro in SOFaa medium at 38.5 �C and 5% CO2 (SF: n ¼27 and VT: n ¼28). Survival rate of SF and VT were, respectively, 29.6% (8/27) and 14.2% (4/28) at 24 h; and 48.1% (13/27) and 32.1% (9/28) at 48 h (P >0.05). Only CDX2 was affected (up-regulated, P <0.05) in both groups compared to CTL. The BAX transcript was upregulated in VT, compared to SF group. The VT increased (P <0.05) the expression of all genes, except for NANOG and NRF1, when compared to the CTL. In conclusion, although in vitro survival was similar between techniques, VT led to increased changes in blastocyst gene expression compared to CTL and SF.
650 $aEmbryo implantation
650 $aEmbryo transfer
650 $aEwes
653 $aApoptosis regulator
653 $aEmbryo cryopreservation
653 $aEmbryonic metabolism
653 $aReproductive biotechnologies
700 1 $aMAIA, A. L. R. S.
700 1 $aCORREIA, L. F. L.
700 1 $aBARBOSA, N. O.
700 1 $aSANTOS, J. D. R.
700 1 $aBRANDÃO, F. Z.
700 1 $aFONSECA, J. F. da
700 1 $aBATISTA, R. I. T. P.
700 1 $aSOUZA-FABJAN, J. M. G.
773 $tCryobiology$gv. 95, p. 110-115, 2020.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
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Registro Completo
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
29/08/2016 |
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Data da última atualização: |
29/05/2017 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
OLIVEIRA, R. P. de; GREGO, C. R. |
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Afiliação: |
RONALDO PEREIRA DE OLIVEIRA, CNPS; CELIA REGINA GREGO, CNPM. |
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Título: |
Adoção da agricultura de precisão. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: OLIVEIRA, R. P. de; GREGO, C. R.; BRANDAO, Z. N. (Ed.). Geoestatística aplicada na agricultura de precisão utilizando o Vesper. Brasília, DF: Embrapa, 2015. cap. 1, p. 19-27. |
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Idioma: |
Português |
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Thesagro: |
Agricultura de precisão. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/146839/1/GeoVesper-cap-1.pdf
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Marc: |
LEADER 00523naa a2200133 a 4500
001 2051862
005 2017-05-29
008 2015 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, R. P. de
245 $aAdoção da agricultura de precisão.$h[electronic resource]
260 $c2015
650 $aAgricultura de precisão
700 1 $aGREGO, C. R.
773 $tIn: OLIVEIRA, R. P. de; GREGO, C. R.; BRANDAO, Z. N. (Ed.). Geoestatística aplicada na agricultura de precisão utilizando o Vesper. Brasília, DF: Embrapa, 2015. cap. 1, p. 19-27.
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