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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
15/03/2000 |
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Data da última atualização: |
10/03/2023 |
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Autoria: |
SANTOS, I. R. I. |
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Título: |
Desiccation and freezing tolerance of embryonic axes and lateral buds of Citrus spp: implication for germplasm conservation. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Fort Collins: Colorado State University, 1999. |
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Páginas: |
159p. |
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Idioma: |
Inglês |
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Notas: |
Ph.D. Thesis. |
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Conteúdo: |
Citrus is the second most importan fruit crop in the world in terms of economic value. Germplasm conservation of commercial cultivars as well as of wild relatives is a priority. Presently, Citrus germplasm has been preserved mainly in field gene banks. Plants maintained in the field are susceptible to biological and climati disasters. Cryopreservation (conservation in liquid nitrogen at -196oC or in its vapor phase at - 150oC) can in theory ensure storage of plant material for several years maintaining high biological and genetic integrity. This research examined several aspects of tolerance to desiccation and freezing in an attempt to develop cryopreseravation protocols for embryonic axes and lateral buds of Pineapple sweet orange (Citrus sinensis [L.] Osb.) and for embryonic axes of Citrus limon Burm. F. and Citrus reticulata Blanco. The roles of alginate encapsulation and pre-culture with sucrose in enhancing tolerance of freezing in liquid nitrogen were tested. Carbohydrate analysis was carried out on axes pre-cultured with sucrose or not and on buds from cold acclimated plants to gain insight on the role of sucrose as a cryopreservation stabilizer. The interrrelationshisp between water content and thermodynamic properties of water during freezing and thawing, which are critical in achieving survival in liquid nitrogen, were also studied. Prior to cryopreservation studies the germiantion of isolated embryonic axes was assessed. For that, embryonic axes with and without alginate encapsulation and with and without sucrose pre-culture, but without dehydration or exposure to liquid nitrogen, were cultured in vitro. Total seedling recovery was observed whether or not axes had been encapsulated and whether normal seedlings, without intermediary callus formation, within three weeks of culture in vitro. MenosCitrus is the second most importan fruit crop in the world in terms of economic value. Germplasm conservation of commercial cultivars as well as of wild relatives is a priority. Presently, Citrus germplasm has been preserved mainly in field gene banks. Plants maintained in the field are susceptible to biological and climati disasters. Cryopreservation (conservation in liquid nitrogen at -196oC or in its vapor phase at - 150oC) can in theory ensure storage of plant material for several years maintaining high biological and genetic integrity. This research examined several aspects of tolerance to desiccation and freezing in an attempt to develop cryopreseravation protocols for embryonic axes and lateral buds of Pineapple sweet orange (Citrus sinensis [L.] Osb.) and for embryonic axes of Citrus limon Burm. F. and Citrus reticulata Blanco. The roles of alginate encapsulation and pre-culture with sucrose in enhancing tolerance of freezing in liquid nitrogen were tested. Carbohydrate analysis was carried out on axes pre-cultured with sucrose or not and on buds from cold acclimated plants to gain insight on the role of sucrose as a cryopreservation stabilizer. The interrrelationshisp between water content and thermodynamic properties of water during freezing and thawing, which are critical in achieving survival in liquid nitrogen, were also studied. Prior to cryopreservation studies the germiantion of isolated embryonic axes was assessed. For that, embryonic axes with and without a... Mostrar Tudo |
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Palavras-Chave: |
Citro; Citrus reticula; Citrus spp; Congelamento Tolerancia; Conservation; Cryopreservatio; Desiccation; Dessidratacao; Embriogenesis; Organges; Plant laboratory; Protocolo; Tolerance. |
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Thesagro: |
Conservação; Criopreservação; Frio; Gema; Germoplasma; Laboratório; Laranja; Limão; Planta. |
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Thesaurus Nal: |
buds; freezing; germplasm; lemons. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02965nam a2200445 a 4500
001 1149518
005 2023-03-10
008 1999 bl uuuu m 00u1 u #d
100 1 $aSANTOS, I. R. I.
245 $aDesiccation and freezing tolerance of embryonic axes and lateral buds of Citrus spp$bimplication for germplasm conservation.
260 $aFort Collins: Colorado State University$c1999
300 $a159p.
500 $aPh.D. Thesis.
520 $aCitrus is the second most importan fruit crop in the world in terms of economic value. Germplasm conservation of commercial cultivars as well as of wild relatives is a priority. Presently, Citrus germplasm has been preserved mainly in field gene banks. Plants maintained in the field are susceptible to biological and climati disasters. Cryopreservation (conservation in liquid nitrogen at -196oC or in its vapor phase at - 150oC) can in theory ensure storage of plant material for several years maintaining high biological and genetic integrity. This research examined several aspects of tolerance to desiccation and freezing in an attempt to develop cryopreseravation protocols for embryonic axes and lateral buds of Pineapple sweet orange (Citrus sinensis [L.] Osb.) and for embryonic axes of Citrus limon Burm. F. and Citrus reticulata Blanco. The roles of alginate encapsulation and pre-culture with sucrose in enhancing tolerance of freezing in liquid nitrogen were tested. Carbohydrate analysis was carried out on axes pre-cultured with sucrose or not and on buds from cold acclimated plants to gain insight on the role of sucrose as a cryopreservation stabilizer. The interrrelationshisp between water content and thermodynamic properties of water during freezing and thawing, which are critical in achieving survival in liquid nitrogen, were also studied. Prior to cryopreservation studies the germiantion of isolated embryonic axes was assessed. For that, embryonic axes with and without alginate encapsulation and with and without sucrose pre-culture, but without dehydration or exposure to liquid nitrogen, were cultured in vitro. Total seedling recovery was observed whether or not axes had been encapsulated and whether normal seedlings, without intermediary callus formation, within three weeks of culture in vitro.
650 $abuds
650 $afreezing
650 $agermplasm
650 $alemons
650 $aConservação
650 $aCriopreservação
650 $aFrio
650 $aGema
650 $aGermoplasma
650 $aLaboratório
650 $aLaranja
650 $aLimão
650 $aPlanta
653 $aCitro
653 $aCitrus reticula
653 $aCitrus spp
653 $aCongelamento Tolerancia
653 $aConservation
653 $aCryopreservatio
653 $aDesiccation
653 $aDessidratacao
653 $aEmbriogenesis
653 $aOrganges
653 $aPlant laboratory
653 $aProtocolo
653 $aTolerance
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Origem |
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Classificação |
Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
18/09/2015 |
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Data da última atualização: |
22/03/2016 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
ANTOLIN, L. A. S.; COSTA NETO, G. M. F.; BORGES, M. G.; HEINEMANN, A. B. |
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Afiliação: |
LUIS ALBERTO S. ANTOLIN, graduando UFG; GERMANO MARTINS F. COSTA NETO, mestrando UFG; M. G. BORGES; ALEXANDRE BRYAN HEINEMANN, CNPAF. |
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Título: |
Mapping the potential yield of upland rice in Rondonia State. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: INOVAGRI INTERNATIONAL MEETING, 3., 2015, Fortaleza. Anais... Fortaleza: INI, 2015. |
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DOI: |
http://dx.doi.org/10.12702/iii.inovagri.2015-a112 |
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Idioma: |
Inglês |
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Conteúdo: |
The rice farming has a high socioeconomical importance for Brazilian population, and represents since the 1970?s, it is the main crop for agricultural borders opening. Aiming characterize the potential yield and to define the regions most favorable for growth upland rice, it was simulated the potential grain yield (PGY), potential evapotranspiration (ETP) and maximum transpiration (TRC) by anoriented process crop model. The simulations were based on cultivar BRSPrimavera, considering eight planting dates (Nov 1st, 10th, 20th, Dec 1st, 10th, 20th, 30th and Jan 9th), for 33 years of data weather (1980-2013), in seven locations at Rondônia state: Ariquemes, Cacoal, Guajara-Mirim, Ji-Paraná, Machadinho do Oeste, Porto Velho and Vilhena. The upland rice crop cycle ranged from 95 days from emergence (DAE) in Ariquemes to 99 DAE in Machadinho do Oeste. The PGY, ETP and TRC data set were spatialized for the region. Rondônia showed an average and standard deviation potential yield of 4393 and 477 kg ha-1. The average ETP was 584,8 mm/cycle, with variance of 1259.9 mm/cycle. Maximum and minimum values of ETP was found for Guajara-Mirim and Vilhena, planting dates of Nov 1st and Jan 9th, respectively. The spatialization of results demonstrated a trend of PGY reduction as the sowing date is delayed. The highest potential yield were found at Vilhena (4952.7 kg/ha) and Ariquemes (4461.4 kg/ha). |
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Palavras-Chave: |
Rondonia. |
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Thesagro: |
Arroz; Evapotranspiração; Oryza sativa; Produtividade. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/129923/1/Inovagri.pdf
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Marc: |
LEADER 02073nam a2200217 a 4500
001 2024348
005 2016-03-22
008 2015 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.12702/iii.inovagri.2015-a112$2DOI
100 1 $aANTOLIN, L. A. S.
245 $aMapping the potential yield of upland rice in Rondonia State.
260 $aIn: INOVAGRI INTERNATIONAL MEETING, 3., 2015, Fortaleza. Anais... Fortaleza: INI$c2015
520 $aThe rice farming has a high socioeconomical importance for Brazilian population, and represents since the 1970?s, it is the main crop for agricultural borders opening. Aiming characterize the potential yield and to define the regions most favorable for growth upland rice, it was simulated the potential grain yield (PGY), potential evapotranspiration (ETP) and maximum transpiration (TRC) by anoriented process crop model. The simulations were based on cultivar BRSPrimavera, considering eight planting dates (Nov 1st, 10th, 20th, Dec 1st, 10th, 20th, 30th and Jan 9th), for 33 years of data weather (1980-2013), in seven locations at Rondônia state: Ariquemes, Cacoal, Guajara-Mirim, Ji-Paraná, Machadinho do Oeste, Porto Velho and Vilhena. The upland rice crop cycle ranged from 95 days from emergence (DAE) in Ariquemes to 99 DAE in Machadinho do Oeste. The PGY, ETP and TRC data set were spatialized for the region. Rondônia showed an average and standard deviation potential yield of 4393 and 477 kg ha-1. The average ETP was 584,8 mm/cycle, with variance of 1259.9 mm/cycle. Maximum and minimum values of ETP was found for Guajara-Mirim and Vilhena, planting dates of Nov 1st and Jan 9th, respectively. The spatialization of results demonstrated a trend of PGY reduction as the sowing date is delayed. The highest potential yield were found at Vilhena (4952.7 kg/ha) and Ariquemes (4461.4 kg/ha).
650 $aArroz
650 $aEvapotranspiração
650 $aOryza sativa
650 $aProdutividade
653 $aRondonia
700 1 $aCOSTA NETO, G. M. F.
700 1 $aBORGES, M. G.
700 1 $aHEINEMANN, A. B.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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