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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
18/03/2020 |
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Data da última atualização: |
02/06/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RAMOS-GONZÁLEZ, P. L.; SANTOS, G, F. dos; CHABI-JESUS, C.; CHABI-JESUS, CAMILA; HARAKAVA, R.; KITAJIMA, E. W.; ASTUA, J. de F. |
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Afiliação: |
PEDRO LUIS RAMOS-GONZÁLEZ, Instituto Biológico; GUSTAVO FRANCISCO DOS SANTOS, Instituto Biológico; CAMILA CHABI-JESUS, Instituto Biológico; CAMILA CHABI-JESUS, Instituto Biológico; RICARDO HARAKAVA, Instituto Biológico; ELLIOT W. KITAJIMA, ESALQ; JULIANA DE FREITAS ASTUA, CNPMF. |
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Título: |
Passion fruit green spot virus genome harbors a new orphan ORF and highlights the flexibility of the 5'-end of the RNA2 segment across cileviruses. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Frontiers in Microbiology, February 2020 . |
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DOI: |
https://doi.org/10.3389/fmicb.2020.00206 |
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Idioma: |
Inglês |
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Conteúdo: |
Passion fruit green spot and passion fruit sudden death are two reportedly distinct viral diseases that recurrently affect passion fruit (Passiflora spp.) groves in Brazil. Here we used a systematic approach that interconnects symptoms, transmission electron microscopy, RT-PCR detection assays followed by Sanger sequencing, and high-throughput sequencing of the RNA of affected passion fruit plants to gain insights about these diseases. Our data confirmed not only the involvement of cileviruses in these two pathologies, as previously suggested, but also that these viruses belong to the same tentative species: passion fruit green spot virus (PfGSV). Results revealed that PfGSV has a positive-sense RNA genome split into two molecules of approximately 9 kb (RNA1) and 5 kb (RNA2), which share about 50?70% nucleotide sequence identity with other viruses in the genus Cilevirus. Genome sequences of five PfGSV isolates suggest that they have more conserved RNA1 (<5% of nucleotide sequence variability) compared to RNA2 (up to 7% of variability) molecules. The highest nucleotide sequence divergence among PfGSV isolates and other cileviruses is in the genomic segment covering from the 5?-end of the RNA2 until the 5?-end of the open reading frame (ORF) p61, which includes the ORF p15 and the intergenic region. This genomic stretch also harbors a novel orphan ORF encoding a 13 kDa protein presenting a cysteine-rich domain. High variability of 5?-end of the RNA2 in cileviruses is discussed in an evolutionary context assuming that they share putative common ancestors with unclassified arthropod-infecting single-strand positive RNA viruses, including mosquito-specific viruses of the group Negevirus (clades Nelorpivirus and Sandwavirus), and other viruses in the family Kitaviridae. MenosPassion fruit green spot and passion fruit sudden death are two reportedly distinct viral diseases that recurrently affect passion fruit (Passiflora spp.) groves in Brazil. Here we used a systematic approach that interconnects symptoms, transmission electron microscopy, RT-PCR detection assays followed by Sanger sequencing, and high-throughput sequencing of the RNA of affected passion fruit plants to gain insights about these diseases. Our data confirmed not only the involvement of cileviruses in these two pathologies, as previously suggested, but also that these viruses belong to the same tentative species: passion fruit green spot virus (PfGSV). Results revealed that PfGSV has a positive-sense RNA genome split into two molecules of approximately 9 kb (RNA1) and 5 kb (RNA2), which share about 50?70% nucleotide sequence identity with other viruses in the genus Cilevirus. Genome sequences of five PfGSV isolates suggest that they have more conserved RNA1 (<5% of nucleotide sequence variability) compared to RNA2 (up to 7% of variability) molecules. The highest nucleotide sequence divergence among PfGSV isolates and other cileviruses is in the genomic segment covering from the 5?-end of the RNA2 until the 5?-end of the open reading frame (ORF) p61, which includes the ORF p15 and the intergenic region. This genomic stretch also harbors a novel orphan ORF encoding a 13 kDa protein presenting a cysteine-rich domain. High variability of 5?-end of the RNA2 in cileviruses is discussed... Mostrar Tudo |
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Thesagro: |
Maracujá. |
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Categoria do assunto: |
-- |
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Marc: |
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024 7 $ahttps://doi.org/10.3389/fmicb.2020.00206$2DOI
100 1 $aRAMOS-GONZÁLEZ, P. L.
245 $aPassion fruit green spot virus genome harbors a new orphan ORF and highlights the flexibility of the 5'-end of the RNA2 segment across cileviruses.$h[electronic resource]
260 $c2020
520 $aPassion fruit green spot and passion fruit sudden death are two reportedly distinct viral diseases that recurrently affect passion fruit (Passiflora spp.) groves in Brazil. Here we used a systematic approach that interconnects symptoms, transmission electron microscopy, RT-PCR detection assays followed by Sanger sequencing, and high-throughput sequencing of the RNA of affected passion fruit plants to gain insights about these diseases. Our data confirmed not only the involvement of cileviruses in these two pathologies, as previously suggested, but also that these viruses belong to the same tentative species: passion fruit green spot virus (PfGSV). Results revealed that PfGSV has a positive-sense RNA genome split into two molecules of approximately 9 kb (RNA1) and 5 kb (RNA2), which share about 50?70% nucleotide sequence identity with other viruses in the genus Cilevirus. Genome sequences of five PfGSV isolates suggest that they have more conserved RNA1 (<5% of nucleotide sequence variability) compared to RNA2 (up to 7% of variability) molecules. The highest nucleotide sequence divergence among PfGSV isolates and other cileviruses is in the genomic segment covering from the 5?-end of the RNA2 until the 5?-end of the open reading frame (ORF) p61, which includes the ORF p15 and the intergenic region. This genomic stretch also harbors a novel orphan ORF encoding a 13 kDa protein presenting a cysteine-rich domain. High variability of 5?-end of the RNA2 in cileviruses is discussed in an evolutionary context assuming that they share putative common ancestors with unclassified arthropod-infecting single-strand positive RNA viruses, including mosquito-specific viruses of the group Negevirus (clades Nelorpivirus and Sandwavirus), and other viruses in the family Kitaviridae.
650 $aMaracujá
700 1 $aSANTOS, G, F. dos
700 1 $aCHABI-JESUS, C.
700 1 $aCHABI-JESUS, CAMILA
700 1 $aHARAKAVA, R.
700 1 $aKITAJIMA, E. W.
700 1 $aASTUA, J. de F.
773 $tFrontiers in Microbiology, February 2020 .
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
13/05/2003 |
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Data da última atualização: |
30/06/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
MATZENBACHER, R. G.; PRIMAVESI, A. C. P. de A.; LAJUS, C. A.; PEGORARO, D.; EMANUELLI, E. P.; ASSAMANN, I.; OLIVEIRA, J. C.; ROSA, J. L. da; ALMEIDA, J. L.; MIRANDA, M.; BASSO, S. M. S. |
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Afiliação: |
R. G. MATZENBACHER, FUNDACEP FECOTRIGO; ANA CANDIDA PACHECO DE A PRIMAVESI, CPPSE; C. A. LAJUS, EPAGRI; D. PEGORARO, PRO SEMENTES; E. P. EMANUELLI, UFSM; I. ASSMANN, CEFET; J. C. OLIVEIRA, IAPAR; J. L. DA ROSA, EPAGRI; J. L. ALMEIDA, FAPA; M. MIRANDA, EPAGRI; S. M. S. BASSO, UPF. |
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Título: |
Ensaio nacional de aveias de cobertura de 2002 - análise conjunta. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
In: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE AVEIA, 23., 2003, Gramado, RS. Anais... Gramado: UFRGS, 2003. |
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Idioma: |
Português |
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Palavras-Chave: |
Analise conjunta; Cobertura; Ensaio nacional. |
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Thesagro: |
Aveia. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPPSE/14439/1/PROCIACP2003.00160.PDF
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Marc: |
LEADER 00823nam a2200265 a 4500
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008 2003 bl uuuu u00u1 u #d
100 1 $aMATZENBACHER, R. G.
245 $aEnsaio nacional de aveias de cobertura de 2002 - análise conjunta.$h[electronic resource]
260 $aIn: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE AVEIA, 23., 2003, Gramado, RS. Anais... Gramado: UFRGS$c2003
650 $aAveia
653 $aAnalise conjunta
653 $aCobertura
653 $aEnsaio nacional
700 1 $aPRIMAVESI, A. C. P. de A.
700 1 $aLAJUS, C. A.
700 1 $aPEGORARO, D.
700 1 $aEMANUELLI, E. P.
700 1 $aASSAMANN, I.
700 1 $aOLIVEIRA, J. C.
700 1 $aROSA, J. L. da
700 1 $aALMEIDA, J. L.
700 1 $aMIRANDA, M.
700 1 $aBASSO, S. M. S.
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Embrapa Pecuária Sudeste (CPPSE) |
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