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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
06/02/2018 |
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Data da última atualização: |
06/02/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUSA, K. C. M. de; FERNANDES, M. P.; HERRERA. H. M.; BENEVENUTE, J. L.; SANTOS, F. M.; BARRETO, W. T. G.; MACEDOB, G. C.; CAMPOSB, J. B.; MARTINS, T. F.; ROCHA, F. L.; PINTOC, C. E. de A.; BATTESTI, D. B.; PIRANDAF, E. M.; CANCADO, P. H. D.; MACHADO, R. Z.; ANDRÉA, M. R. |
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Afiliação: |
Keyla Carstens Marques de Sousa, Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista/UNESP; Marina Pugnaghi Fernandes, Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista/UNESP; Heitor Miraglia Herrera, Universidade Católica Dom Bosco; Jyan Lucas Benevenute, Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista/UNESP; Filipe Martins Santos, Universidade Católica Dom Bosco; Wanessa Teixeira Gomes Barreto, Universidade Católica Dom Bosco; Gabriel Carvalho Macedob, Universidade Católica Dom Bosco; João Bosco Camposb, Universidade Católica Dom Bosco; Thiago Fernandes Martins, Faculdade de Medicina Veterinária e Zootecnia/Universidade de São Paulo; Fabiana Lopes Rocha, Laboratório de Ecologia Animal/Universidade Federal da Paraíba; Pedro Cordeiro Estrela de Andrade Pintoc, Laboratório de Ecologia Animal/Universidade Federal da Paraíba; Darci Barros Battesti, Laboratório de Parasitologia/Instituto Butantan; Eliane Mattos Pirandaf, Universidade Federal de Mato Grosso do Sul; PAULO HENRIQUE DUARTE CANCADO, CNPGC; Rosangela Zacarias Machado, Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista/UNESP; Marcos Rogério Andréa, Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista/UNESP. |
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Título: |
Molecular detection of Hepatozoon spp. in domestic dogs and wild mammals in southern Pantanal, Brazil with implications in the transmission route. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Veterinary Parasitology, v. 237, p. 37-46, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Hepatozoon parasites comprise intracellular apicomplexan parasites transmitted to vertebrate animals by ingestion of arthropods definitive hosts. The present work aimed to investigate the occurrence of Hepatozoon spp. in wild animals, domestic dogs and their respective ectoparasites, in southern Pantanal region, central-western Brazil, by molecular techniques. Between August 2013 and March 2015, 31 coatis (Nasua nasua), 78 crab-eating foxes (Cerdocyon thous), seven ocelots (Leopardus pardalis), 42 dogs (Canis lupus familiaris), 110 wild rodents (77 Thichomys fosteri, 25 Oecomys mamorae, and 8 Clyomys laticeps), 30 marsupials (14 Thylamys macrurus, 11 Gracilinanus agilis, 4 Monodelphis domestica and 1 Didelphis albiventris), and 1582 ticks and 80 fleas collected from the sampled animals were investigated. DNA samples were submitted to PCR assays for Hepatozoon spp. targeting 18S rRNA gene. Purified amplicons were directly sequenced and submitted to phylogenetic analysis. A high prevalence of Hepatozoon among carnivores (C. thous [91.02%], dogs [45.23%], N. nasua [41.9%] and L. pardalis [71.4%]) was found. However, ticks and fleas were negative to Hepatozoon PCR assays. By phylogenetic analysis based on 18S rRNA sequences, Hepatozoon sequences amplified from crab-eating foxes, dogs, coatis and ocelots clustered with sequences of H. canis, H. americanum and H. felis. The closely related positioning of Hepatozoon sequences amplified from wild rodents and T. macrurus marsupial to Hepatozoon from reptiles and amphibians suggest a possible transmission of those Hepatozoon species between hosts by ectoparasites or by predation. Hepatozoon haplotypes found circulating in wild rodents seem to present a higher degree of polymorphism when compared to those found in other groups of animals. Although rodents seem not to participate as source of Hepatozoon infection to wild carnivores and domestic dogs, they may play an important role in the transmission of Hepatozoon to reptiles and amphibians in Pantanal biome. MenosHepatozoon parasites comprise intracellular apicomplexan parasites transmitted to vertebrate animals by ingestion of arthropods definitive hosts. The present work aimed to investigate the occurrence of Hepatozoon spp. in wild animals, domestic dogs and their respective ectoparasites, in southern Pantanal region, central-western Brazil, by molecular techniques. Between August 2013 and March 2015, 31 coatis (Nasua nasua), 78 crab-eating foxes (Cerdocyon thous), seven ocelots (Leopardus pardalis), 42 dogs (Canis lupus familiaris), 110 wild rodents (77 Thichomys fosteri, 25 Oecomys mamorae, and 8 Clyomys laticeps), 30 marsupials (14 Thylamys macrurus, 11 Gracilinanus agilis, 4 Monodelphis domestica and 1 Didelphis albiventris), and 1582 ticks and 80 fleas collected from the sampled animals were investigated. DNA samples were submitted to PCR assays for Hepatozoon spp. targeting 18S rRNA gene. Purified amplicons were directly sequenced and submitted to phylogenetic analysis. A high prevalence of Hepatozoon among carnivores (C. thous [91.02%], dogs [45.23%], N. nasua [41.9%] and L. pardalis [71.4%]) was found. However, ticks and fleas were negative to Hepatozoon PCR assays. By phylogenetic analysis based on 18S rRNA sequences, Hepatozoon sequences amplified from crab-eating foxes, dogs, coatis and ocelots clustered with sequences of H. canis, H. americanum and H. felis. The closely related positioning of Hepatozoon sequences amplified from wild rodents and T. macrurus marsupial to... Mostrar Tudo |
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Palavras-Chave: |
Hepatozoon spp. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/172251/1/Molecular-detection-of-Hepatozoon-spp.-in-domestic-dogs-and-wild.pdf
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Marc: |
LEADER 02990naa a2200313 a 4500
001 2087229
005 2018-02-06
008 2017 bl uuuu u00u1 u #d
100 1 $aSOUSA, K. C. M. de
245 $aMolecular detection of Hepatozoon spp. in domestic dogs and wild mammals in southern Pantanal, Brazil with implications in the transmission route.$h[electronic resource]
260 $c2017
520 $aHepatozoon parasites comprise intracellular apicomplexan parasites transmitted to vertebrate animals by ingestion of arthropods definitive hosts. The present work aimed to investigate the occurrence of Hepatozoon spp. in wild animals, domestic dogs and their respective ectoparasites, in southern Pantanal region, central-western Brazil, by molecular techniques. Between August 2013 and March 2015, 31 coatis (Nasua nasua), 78 crab-eating foxes (Cerdocyon thous), seven ocelots (Leopardus pardalis), 42 dogs (Canis lupus familiaris), 110 wild rodents (77 Thichomys fosteri, 25 Oecomys mamorae, and 8 Clyomys laticeps), 30 marsupials (14 Thylamys macrurus, 11 Gracilinanus agilis, 4 Monodelphis domestica and 1 Didelphis albiventris), and 1582 ticks and 80 fleas collected from the sampled animals were investigated. DNA samples were submitted to PCR assays for Hepatozoon spp. targeting 18S rRNA gene. Purified amplicons were directly sequenced and submitted to phylogenetic analysis. A high prevalence of Hepatozoon among carnivores (C. thous [91.02%], dogs [45.23%], N. nasua [41.9%] and L. pardalis [71.4%]) was found. However, ticks and fleas were negative to Hepatozoon PCR assays. By phylogenetic analysis based on 18S rRNA sequences, Hepatozoon sequences amplified from crab-eating foxes, dogs, coatis and ocelots clustered with sequences of H. canis, H. americanum and H. felis. The closely related positioning of Hepatozoon sequences amplified from wild rodents and T. macrurus marsupial to Hepatozoon from reptiles and amphibians suggest a possible transmission of those Hepatozoon species between hosts by ectoparasites or by predation. Hepatozoon haplotypes found circulating in wild rodents seem to present a higher degree of polymorphism when compared to those found in other groups of animals. Although rodents seem not to participate as source of Hepatozoon infection to wild carnivores and domestic dogs, they may play an important role in the transmission of Hepatozoon to reptiles and amphibians in Pantanal biome.
653 $aHepatozoon spp
700 1 $aFERNANDES, M. P.
700 1 $aHERRERA. H. M.
700 1 $aBENEVENUTE, J. L.
700 1 $aSANTOS, F. M.
700 1 $aBARRETO, W. T. G.
700 1 $aMACEDOB, G. C.
700 1 $aCAMPOSB, J. B.
700 1 $aMARTINS, T. F.
700 1 $aROCHA, F. L.
700 1 $aPINTOC, C. E. de A.
700 1 $aBATTESTI, D. B.
700 1 $aPIRANDAF, E. M.
700 1 $aCANCADO, P. H. D.
700 1 $aMACHADO, R. Z.
700 1 $aANDRÉA, M. R.
773 $tVeterinary Parasitology$gv. 237, p. 37-46, 2017.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
23/11/2011 |
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Data da última atualização: |
11/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
DELGADO, J. V.; MARTÍNEZ, A. M.; ACOSTA, A.; ALVAREZ, L. A.; ARMSTRONG, E.; CAMACHO, E.; CAÑON, J.; CORTÉS, O.; DUNNER, S.; LANDI, V.; MARQUES, J. R.; MARTÍN-BURRIEL, I.; MARTÍNEZ, O. R.; MARTÍNEZ, R. D.; MELUCCI, L.; MUÑOZ, J. E.; PENEDO, M. C. T.; POSTIGLIONI, A.; QUIRÓZ, J.; RODELLAR, C.; SPONENBERG, P.; UFFO, O.; ULLOA-ARVIZU, R.; VEGA-PLA, J. L.; VILLALOBOS, A.; ZAMBRANO, D.; ZARAGOZA, P.; GAMA, L. T.; GINJA, C. |
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Afiliação: |
UNIVERSIDAD DE CÓRDOBA; UNIVERISDAD DE CÓRDOBA; CENSA; UNIVERSIDAD NACIONAL DE COLOMBIA; FACULTAD DE VETERINARIA-U DE LA R; IFAPA; UNIVERSIDAD COMPLUTENSE DE MADRID; UNIVERSIDAD COMPLUTENSE DE MADRID; UNIVERSIDAD COMPLUTENSE DE MADRID; UNIVERSIDAD DE CÓRDOBA; JOSE RIBAMAR FELIPE MARQUES, CPATU; UNIVERISDAD DE ZARAGOZA; UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO / UNIVERSIDAD NACIONAL DE ASUNCIÓN; UNIVERSIDAD NACIONAL DE LOMAS DE ZAMORA; UNIVERSIDAD NACIONAL DE MAR DEL PLATA / INSTITUTO NACIONAL DE TECNOLOGIA AGROPECUARIA; UNIVERSIDAD NACIONAL DE COLOMBIA; UNIVERSITY OF CALIFORNIA; FACULTAD DE VETERINARIA - U DE LA R; INSTITUTO NACIONAL DE INVESTIGACIONES FORESTALES, AGRICOLAS Y PECUARIAS; UNIVERSIDAD DE ZARAGOZA; VIRGINIA-MARYLAND REGIONAL COLLEGE OF VETERINARY MEDICINE; CENSA; UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO; CRIA CABALLAR DE LAS FUERZAS ARMADAS; INSTITUTO DE INVESTIGACION AGROPECUARIA; UNIVERSIDAD TÉCNICO ESTATAL DE QUEVEDO; UNIVERSIDAD DE ZARAGOZA; INSTITUTO NACIONAL DOS RECURSOS BIOLÓGICOS; INSTITUTO SUPERIOR DE AGRONOMIA / INSTITUTO NACIONAL DOS RECURSOS BIOLÓGICOS / UNIVERSITY OF CALIFORNIA. |
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Título: |
Genetic characterization of Latin-American creole cattle using microsatellite markers. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Animal Genetics, v. 43, n. 1, p. 2-10, Feb. 2012. |
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DOI: |
10.1111/j.1365-2052.2011.02207.x |
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Idioma: |
Inglês |
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Conteúdo: |
Genetic diversity in and relationships among 26 Creole cattle breeds from 10 American countries were assessed using 19 microsatellites. Heterozygosities, F-statistics estimates, genetic distances, multivariate analyses and assignment tests were performed. The levels of within-breed diversity detected in Creole cattle were considerable and higher than those previously reported for European breeds, but similar to those found in other Latin American breeds. Differences among breeds accounted for 8.4% of the total genetic variability. Most breeds clustered separately when the number of pre-defined populations was 21 (the most probable K value), with the exception of some closely related breeds that shared the same cluster and others that were admixed. Despite the high genetic diversity detected, significant inbreeding was also observed within some breeds, and heterozygote excess was detected in others. These results indicate that Creoles represent important reservoirs of cattle genetic diversity and that appropriate conservation measures should be implemented for these native breeds in order to minimize inbreeding and uncontrolled crossbreeding. |
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Palavras-Chave: |
Caracterização genética. |
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Thesagro: |
Gado. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02494naa a2200493 a 4500
001 1906892
005 2022-11-11
008 2012 bl uuuu u00u1 u #d
024 7 $a10.1111/j.1365-2052.2011.02207.x$2DOI
100 1 $aDELGADO, J. V.
245 $aGenetic characterization of Latin-American creole cattle using microsatellite markers.$h[electronic resource]
260 $c2012
520 $aGenetic diversity in and relationships among 26 Creole cattle breeds from 10 American countries were assessed using 19 microsatellites. Heterozygosities, F-statistics estimates, genetic distances, multivariate analyses and assignment tests were performed. The levels of within-breed diversity detected in Creole cattle were considerable and higher than those previously reported for European breeds, but similar to those found in other Latin American breeds. Differences among breeds accounted for 8.4% of the total genetic variability. Most breeds clustered separately when the number of pre-defined populations was 21 (the most probable K value), with the exception of some closely related breeds that shared the same cluster and others that were admixed. Despite the high genetic diversity detected, significant inbreeding was also observed within some breeds, and heterozygote excess was detected in others. These results indicate that Creoles represent important reservoirs of cattle genetic diversity and that appropriate conservation measures should be implemented for these native breeds in order to minimize inbreeding and uncontrolled crossbreeding.
650 $aGado
653 $aCaracterização genética
700 1 $aMARTÍNEZ, A. M.
700 1 $aACOSTA, A.
700 1 $aALVAREZ, L. A.
700 1 $aARMSTRONG, E.
700 1 $aCAMACHO, E.
700 1 $aCAÑON, J.
700 1 $aCORTÉS, O.
700 1 $aDUNNER, S.
700 1 $aLANDI, V.
700 1 $aMARQUES, J. R.
700 1 $aMARTÍN-BURRIEL, I.
700 1 $aMARTÍNEZ, O. R.
700 1 $aMARTÍNEZ, R. D.
700 1 $aMELUCCI, L.
700 1 $aMUÑOZ, J. E.
700 1 $aPENEDO, M. C. T.
700 1 $aPOSTIGLIONI, A.
700 1 $aQUIRÓZ, J.
700 1 $aRODELLAR, C.
700 1 $aSPONENBERG, P.
700 1 $aUFFO, O.
700 1 $aULLOA-ARVIZU, R.
700 1 $aVEGA-PLA, J. L.
700 1 $aVILLALOBOS, A.
700 1 $aZAMBRANO, D.
700 1 $aZARAGOZA, P.
700 1 $aGAMA, L. T.
700 1 $aGINJA, C.
773 $tAnimal Genetics$gv. 43, n. 1, p. 2-10, Feb. 2012.
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