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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
18/04/2024 |
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Data da última atualização: |
18/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, E. R. dos; CAMARGO, B. R. de; SILVA, L. A. da; LAUMANN, R. A.; RIBEIRO, B. M.; ARDISSON-ARAÚJO, D. M. P. |
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Afiliação: |
ETHIANE ROZO DOS SANTOS, UNIVERSIDADE DE BRASÍLIA; BRENDA RABELO DE CAMARGO, UNIVERSIDADE DE BRASÍLIA; LEONARDO ASSIS DA SILVA, UNIVERSIDADE DE BRASÍLIA; RAUL ALBERTO LAUMANN, CENARGEN; BERGMANN MORAIS RIBEIRO, UNIVERSIDADE DE BRASÍLIA; DANIEL M. P. ARDISSON-ARAÚJO, UNIVERSIDADE DE BRASÍLIA. |
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Título: |
The multispecies stinkbug iflavirus Halyomorpha halys virus detected in the multispecies stinkbug egg parasitoid microwasp, Telenomus podisi (Ashmead) (Hymenoptera: Platygastridae). |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, 2024. |
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DOI: |
https://doi.org/10.1007/s42770-024-01340-y |
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Idioma: |
Português |
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Notas: |
On-line first. |
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Conteúdo: |
Wasps are important parasitoids of stinkbugs and frequently exposed to various types of microorganisms through environmental contact and fecal–oral transmission route. Many parasitize stinkbug eggs and are commercially used in the field to control insect population. The parasitoid T. podisi is known for its high parasitism capacity and ability to target multiple species of stinkbugs. In this study we asked whether T. podisi exposed to eggs infected by a multispecies asymptomatic stinkbug virus, the Halyomorpha halys virus (HhV) would get infected. HhV is a geographically distributed multispecies iflavirus previously found to infect four stinkbug hosts, including three Brazilian species, Chinavia ubica, Euschistus heros and Diceraeus melacanthus, and T. posidi can parasitize all of them. As results, RT-PCR screening revealed positive samples for the HhV genome in two out of four tested pools of T. podisi, whereas the antigenome, indicative of replicative activity, was not detected. The wasps were raised in E. heros eggs that presented both the genome and the antigenome forms of the HhV genome. Subsequent RNA-deep sequencing of HhV positive T. podisi RNA pools yielded a complete genome of HhV with high coverage. Phylogenetic analysis positioned the isolate HhV-Tp (isolate Telenomus podisi) alongside with the stinkbug HhV. Analysis of transcriptomes from several hymenopteran species revealed HhV-Tp reads in four species. However, the transmission mechanism and the ecological significance of HhV remain elusive, warranting further studies to illuminate both the transmission process and its capacity for environmental propagation using T. podisi as a potential vector. MenosWasps are important parasitoids of stinkbugs and frequently exposed to various types of microorganisms through environmental contact and fecal–oral transmission route. Many parasitize stinkbug eggs and are commercially used in the field to control insect population. The parasitoid T. podisi is known for its high parasitism capacity and ability to target multiple species of stinkbugs. In this study we asked whether T. podisi exposed to eggs infected by a multispecies asymptomatic stinkbug virus, the Halyomorpha halys virus (HhV) would get infected. HhV is a geographically distributed multispecies iflavirus previously found to infect four stinkbug hosts, including three Brazilian species, Chinavia ubica, Euschistus heros and Diceraeus melacanthus, and T. posidi can parasitize all of them. As results, RT-PCR screening revealed positive samples for the HhV genome in two out of four tested pools of T. podisi, whereas the antigenome, indicative of replicative activity, was not detected. The wasps were raised in E. heros eggs that presented both the genome and the antigenome forms of the HhV genome. Subsequent RNA-deep sequencing of HhV positive T. podisi RNA pools yielded a complete genome of HhV with high coverage. Phylogenetic analysis positioned the isolate HhV-Tp (isolate Telenomus podisi) alongside with the stinkbug HhV. Analysis of transcriptomes from several hymenopteran species revealed HhV-Tp reads in four species. However, the transmission mechanism and the ecological si... Mostrar Tudo |
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Palavras-Chave: |
Egg parasitoid; Halyomorpha halys virus; Stinkbug; Telenomus podisi. |
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Categoria do assunto: |
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Marc: |
LEADER 02558naa a2200253 a 4500
001 2163741
005 2024-04-18
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s42770-024-01340-y$2DOI
100 1 $aSANTOS, E. R. dos
245 $aThe multispecies stinkbug iflavirus Halyomorpha halys virus detected in the multispecies stinkbug egg parasitoid microwasp, Telenomus podisi (Ashmead) (Hymenoptera$bPlatygastridae).$h[electronic resource]
260 $c2024
500 $aOn-line first.
520 $aWasps are important parasitoids of stinkbugs and frequently exposed to various types of microorganisms through environmental contact and fecal–oral transmission route. Many parasitize stinkbug eggs and are commercially used in the field to control insect population. The parasitoid T. podisi is known for its high parasitism capacity and ability to target multiple species of stinkbugs. In this study we asked whether T. podisi exposed to eggs infected by a multispecies asymptomatic stinkbug virus, the Halyomorpha halys virus (HhV) would get infected. HhV is a geographically distributed multispecies iflavirus previously found to infect four stinkbug hosts, including three Brazilian species, Chinavia ubica, Euschistus heros and Diceraeus melacanthus, and T. posidi can parasitize all of them. As results, RT-PCR screening revealed positive samples for the HhV genome in two out of four tested pools of T. podisi, whereas the antigenome, indicative of replicative activity, was not detected. The wasps were raised in E. heros eggs that presented both the genome and the antigenome forms of the HhV genome. Subsequent RNA-deep sequencing of HhV positive T. podisi RNA pools yielded a complete genome of HhV with high coverage. Phylogenetic analysis positioned the isolate HhV-Tp (isolate Telenomus podisi) alongside with the stinkbug HhV. Analysis of transcriptomes from several hymenopteran species revealed HhV-Tp reads in four species. However, the transmission mechanism and the ecological significance of HhV remain elusive, warranting further studies to illuminate both the transmission process and its capacity for environmental propagation using T. podisi as a potential vector.
653 $aEgg parasitoid
653 $aHalyomorpha halys virus
653 $aStinkbug
653 $aTelenomus podisi
700 1 $aCAMARGO, B. R. de
700 1 $aSILVA, L. A. da
700 1 $aLAUMANN, R. A.
700 1 $aRIBEIRO, B. M.
700 1 $aARDISSON-ARAÚJO, D. M. P.
773 $tBrazilian Journal of Microbiology, 2024.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| Registros recuperados : 53 | |
| 5. |  | MACHADO, M. A. C. F; SANTOS, E. R. dos; BETTIOL, W.; MORANDI, M. A. B. Antagonismo de Sporidiobolus pararoseus a Botrytis cinerea em discos de folha de lírio originária de produção integrada. Tropical Plant Pathology, Brasília, DF, v. 34, p. S70, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia; XLII Annual Meeting of the Brazilian Pthytopathologiacal Society, Rio de Janeiro, ago. 2009. Resumo 252.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 8. | | STELA, E. P.; MORANDI, M. A. B.; SANTOS, E. R. dos; CAOVILA, L. E.; FERNANDES, M. Comparação de formulações de Clonostachys rosea no biocontrole de Botrytis cinerea. In: Summa Phytopathologica, Botucatu, v. 32, supl., p. S.38, fev. 2006.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 9. | | MORANDI, M. A. B.; MATTOS, L. P. V.; SANTOS, E. R. dos. Efeito da hora de aplicação sobre a eficiência de Clonostachys rosea em controlar Botrytis cinerea em roseiras. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 39., 2006, Salvador, BA. Resumos... Salvador, BA: SBF, 2006. Revista Fitopatologia Brasileira, Brasília, DF, v.31, supl., p. S.137, ago. 2006.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 12. | | BONUGLI, R. C.; MORANDI, M. A. B.; MATTOS, L. P. V.; SANTOS, E. R. dos. Efeito da radiação solar na sobrevivência e estabelecimento de clonostachys rosea, agente de biocontrole de botrytis cinerea em roseiras. Fitopatologia Brasileira, Brasília, v. 29, p. 72, ago. 2004. Suplemento. R 261. Edição de Resumos do 37º Congresso Brasileiro de Fitopatologia, ago. 2004. p. S72| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 13. | | MORANDI, M. A. B.; MATTOS, L. P. V.; SANTOS, E. R. dos; BONUGLI, R. C. Influence of application time on the establishment, survival, and ability of Clonostachys rosea to suppress Botrytis cinerea sporulation on rose debris. Crop Protection, Guildford, v. 27, n.1, p. 77-83, 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 14. | | MORANDI, M. A. B.; MATTOS, L. P. V.; SANTOS, E. R. dos. Influence of application time on survival, establishment and ability of Clonostachys rosea to control Botrytis cinerea conidiation on rose debris. In: MEETING OF THE WORKING GROUP ''BIOLOGICAL CONTROL OF FUNGAL AND BACTERIAL PLANT PATHOGENS", 9., 2006, Spa, Belgium. Proceedings... IOBC/WPRS Bulletin, v.30, n.6, p.317-320, 2007.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 18. | | COSTA, L. B.; MORANDI, M. A. B.; SANTOS, E. R. dos. Redução do inóculo de Sclerotinia sclerotiorum por Trichoderma asperellum e Clonostachys rosea em feijão de inverno nas safras 2007 e 2008. Tropical Plant Pathology, Brasília, DF, v. 34, p. S70, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia; XLII Annual Meeting of the Brazilian Pthytopathologiacal Society, Rio de Janeiro, ago. 2009. Resumo 253.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| Registros recuperados : 53 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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