Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
02/06/2022 |
Data da última atualização: |
02/06/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SANTOS, E. M. dos; TAVARES, L. S.; FAYER, L.; BRANDAO, H. de M.; MUNK, M.; SANTOS, M. de O. |
Afiliação: |
ELISANDRA M. DOS SANTOS, Universidade Federal de Juiz de Fora; LETÍCIA S. TAVARES, Universidade Federal de Juiz de Fora; LEONARA FAYER, Universidade Federal de Juiz de Fora; HUMBERTO DE MELLO BRANDAO, CNPGL; MICHELE MUNK, Universidade Federal de Juiz de Fora; MARCELO DE O. SANTOS, Universidade Federal de Juiz de Fora. |
Título: |
Nanoencapsulated Lippia rotundifolia antimicrobial peptide: synthesis, characterization, antimicrobial activity, and cytotoxicity evaluations. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Archives of Microbiology, v. 204, n. 3, 184, 2022. |
DOI: |
https://doi.org/10.1007/s00203-022-02787-z |
Idioma: |
Inglês |
Conteúdo: |
Antimicrobial peptides (AMP) are promising novel antibiotics but exhibit low stability and can be toxic. The AMP encapsulation can be used to protect the drug and control its release rates. The Lr-AMP1f encapsulated into chitosan nanoparticle (NP) by ionic gelation method reached 90% efficiency. The results indicated that the hydrodynamic particle size of NPs increased from 196.1 ± 3.14 nm (free NP) to 228.1 ± 12.22 nm (nanoencapsulated Lr-AMP1f), while the atomic force microscope showed the spherical shape. The Zeta potential of the nanoencapsulated Lr-AMP1f was high (+ 35 mV). These AMP-loaded NPs exhibited stability for up to 21 days of storage. The minimum inhibitory concentration (MIC) of free Lr-AMP1f was 8 µg/mL for E. coli and S. epidermidis. However, the nanoencapsulated Lr-AMP1f produced a bacteriostatic effect against both bacteria at 8 µg/mL. The MIC of nanoencapsulated Lr-AMP1f was 16 µg/mL for E. coli and 32 for S. epidermidis. Nanoencapsulated Lr-AMP1f was nontoxic to HEK293 cells. Promisingly, chitosan NP can be used as a vehicle for the antibacterial application of new AMP (Lr-AMP1f). |
Palavras-Chave: |
Atividade antimicrobiana; Citotoxicidade; Nanocapsulado; Nanotecnologia; Peptídeo antimicrobiano. |
Thesagro: |
Antibiótico; Peptídeo. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02008naa a2200277 a 4500 001 2143648 005 2022-06-02 008 2022 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s00203-022-02787-z$2DOI 100 1 $aSANTOS, E. M. dos 245 $aNanoencapsulated Lippia rotundifolia antimicrobial peptide$bsynthesis, characterization, antimicrobial activity, and cytotoxicity evaluations.$h[electronic resource] 260 $c2022 520 $aAntimicrobial peptides (AMP) are promising novel antibiotics but exhibit low stability and can be toxic. The AMP encapsulation can be used to protect the drug and control its release rates. The Lr-AMP1f encapsulated into chitosan nanoparticle (NP) by ionic gelation method reached 90% efficiency. The results indicated that the hydrodynamic particle size of NPs increased from 196.1 ± 3.14 nm (free NP) to 228.1 ± 12.22 nm (nanoencapsulated Lr-AMP1f), while the atomic force microscope showed the spherical shape. The Zeta potential of the nanoencapsulated Lr-AMP1f was high (+ 35 mV). These AMP-loaded NPs exhibited stability for up to 21 days of storage. The minimum inhibitory concentration (MIC) of free Lr-AMP1f was 8 µg/mL for E. coli and S. epidermidis. However, the nanoencapsulated Lr-AMP1f produced a bacteriostatic effect against both bacteria at 8 µg/mL. The MIC of nanoencapsulated Lr-AMP1f was 16 µg/mL for E. coli and 32 for S. epidermidis. Nanoencapsulated Lr-AMP1f was nontoxic to HEK293 cells. Promisingly, chitosan NP can be used as a vehicle for the antibacterial application of new AMP (Lr-AMP1f). 650 $aAntibiótico 650 $aPeptídeo 653 $aAtividade antimicrobiana 653 $aCitotoxicidade 653 $aNanocapsulado 653 $aNanotecnologia 653 $aPeptídeo antimicrobiano 700 1 $aTAVARES, L. S. 700 1 $aFAYER, L. 700 1 $aBRANDAO, H. de M. 700 1 $aMUNK, M. 700 1 $aSANTOS, M. de O. 773 $tArchives of Microbiology$gv. 204, n. 3, 184, 2022.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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