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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
10/10/2013 |
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Data da última atualização: |
09/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BEVITORI, R.; POPIELARSKA-KONIECZNA, M.; SANTOS, E. M. dos; GROSSI-DE-SÁ, M. F.; PETROFEZA, S. |
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Afiliação: |
ROSANGELA BEVITORI, CNPAF; M. POPIELARSKA-KONIECZNA, JAGIELLONIAN UNIVERSITY, CRACOW-POLAND; E. M. DOS SANTOS, UFG; MARIA FATIMA GROSSI DE SA, CENARGEN; S. PETROFEZA, UFG. |
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Título: |
Morpho-anatomical characterization of mature embryo-derived callus of rice (Oryza sativa L.) suitable for transformation. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Protoplasma, New York, v. 251, n. 3, p. 545-554, May 2014. |
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DOI: |
10.1007/s00709-013-0553-4 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to morphoanatomically characterize embryogenic rice calli during early induction of somatic embryogenesis of three Brazilian rice cultivars. Herein, we explored embryogenic units (EUs) from 2-week-old cut proliferated calli to verify whether they were suitable for Agrobacterium tumefasciens-mediated transformation. Histological analysis and scanning electron microscopy (SEM) were used to analyze these types of calli during early rice callogenesis in the cultivars BRS Primavera, BRS Bonança, and BRS Caiapó. The characteristics of the embryogenic cells were preserved in the EUs, which showed a globular, compact structure that contained tightly packed cells and thus rendered the cells suitable for transformation. The EUs of BRS Caiapó also maintained the characteristics of the nonembryogenic callus, such as an elongated morphology and a lack of cellular organization. In general, the observations of the histological sections corresponded with those of the SEM images. The histological analysis suggested that all cultivars used in these experiments have morphogenic potential. The EUs from proliferated 2-week-old cut calli maintained their embryogenic features. The EUs were subjected to Agrobacterium-mediated transformation, which exhibited a regeneration frequency of 58 % for transformed hygromycin-resistant cell lines. These results show that EUs from proliferated 2-week-old cut calli are suitable for plant transformation. |
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Thesagro: |
Arroz; Embriogenese; Morfogenese; Oryza sativa. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/179857/1/Bevitori2014-Article-Morpho-anatomicalCharacterizat.pdf
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Marc: |
LEADER 02199naa a2200229 a 4500
001 1975223
005 2023-03-09
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1007/s00709-013-0553-4$2DOI
100 1 $aBEVITORI, R.
245 $aMorpho-anatomical characterization of mature embryo-derived callus of rice (Oryza sativa L.) suitable for transformation.$h[electronic resource]
260 $c2014
520 $aThe objective of this study was to morphoanatomically characterize embryogenic rice calli during early induction of somatic embryogenesis of three Brazilian rice cultivars. Herein, we explored embryogenic units (EUs) from 2-week-old cut proliferated calli to verify whether they were suitable for Agrobacterium tumefasciens-mediated transformation. Histological analysis and scanning electron microscopy (SEM) were used to analyze these types of calli during early rice callogenesis in the cultivars BRS Primavera, BRS Bonança, and BRS Caiapó. The characteristics of the embryogenic cells were preserved in the EUs, which showed a globular, compact structure that contained tightly packed cells and thus rendered the cells suitable for transformation. The EUs of BRS Caiapó also maintained the characteristics of the nonembryogenic callus, such as an elongated morphology and a lack of cellular organization. In general, the observations of the histological sections corresponded with those of the SEM images. The histological analysis suggested that all cultivars used in these experiments have morphogenic potential. The EUs from proliferated 2-week-old cut calli maintained their embryogenic features. The EUs were subjected to Agrobacterium-mediated transformation, which exhibited a regeneration frequency of 58 % for transformed hygromycin-resistant cell lines. These results show that EUs from proliferated 2-week-old cut calli are suitable for plant transformation.
650 $aArroz
650 $aEmbriogenese
650 $aMorfogenese
650 $aOryza sativa
700 1 $aPOPIELARSKA-KONIECZNA, M.
700 1 $aSANTOS, E. M. dos
700 1 $aGROSSI-DE-SÁ, M. F.
700 1 $aPETROFEZA, S.
773 $tProtoplasma, New York$gv. 251, n. 3, p. 545-554, May 2014.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| 12. |  | SANTOS, E. M. R.; FRANKLIN, E.; LUIZÃO, F. J. Litter manipulation and associated invertebrate fauna in secondary forest, central Amazonia, Brazil. In: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM.| Biblioteca(s): Embrapa Florestas. |
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| 17. | | CUENCA, M. A. G.; GABRIEL, T. dos S.; PRATA, D. A. T.; SANTOS, E. M. Análise econométrica do comportamento histórico dos preços pagos aos cocoicultores sergipanos e sua participação percentual na variação do valor bruto da produção de coco. CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL HOTRICULTURE, 54., 2008, Vitória. Frutas para todos: estratégias, tecnologias e visão sustentável. Vitória: Sociedade Brasileira de Fruticultura, 2008. Custos, comercialização e mercado. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
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| 18. | | CUENCA, M. A. G.; GABRIEL, T. dos S.; PRATA, D. A. T.; SANTOS, E. M. Análise da tendência histórica de preços recebidos pelos produtores de laranja no estado de Sergipe e sua participação percentual na variação do valor bruto da produção citrícola estadual. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL HOTRICULTURE, 54., 2008, Vitória. Frutas para todos: estratégias, tecnologias e visão sustentável. Vitória: Sociedade Brasileira de Fruticultura, 2008. Custos, comercialização e mercado. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| Registros recuperados : 121 | |
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