Data/hora: 24/05/2024 15:34:36

Biblioteca(s): 	Embrapa Unidades Centrais.	
Data corrente: 	15/02/2016	
Data da última atualização: 	15/02/2016	
Autoria: 	SANTOS, E. D.; SILVA FILHO, J. R.; MACHADO, T. P.; DAU, S. L; RODRIGUEZ, R.; MOTTA, A C. da.	
Afiliação: 	EZEQUIEL D. SANTOS, FAMV; JOSÉ R. SILVA FILHO, Universidade de Passo Fundo; TANISE P. MACHADO, FAMV; STEFANO L. DAU; RUBENS RODRIGUEZ, IPPF; ADRIANA C. da MOTTA, FAMV.	
Título: 	Oral fibrosarcoma in jararaca (Bothrops pubescens): anatomopathological and immunohistochemical aspects.	
Ano de publicação: 	2015	
Fonte/Imprenta: 	Pesquisa Veterinária Brasileira v. 35, n.7, p. 664-670, julho 2015.	
Idioma: 	Inglês	
Conteúdo: 	A 4-year-old female captive-bred snake of the genus Bothrops showed swelling on the left side of the oral cavity, suggesting the development of neoplasia. The mass was removed surgically and sent for pathological examination. Two months later a new increase in volume in the same site was observed, suggesting recurrence. The lesion was completely removed and sent for pathological analysis. Histologically, the two-samples consisted of a mass with highly-cell density composed of spindle-shaped anaplastic cells arranged in interwoven bundles, distributed throughout the tissue extension and, occasionally, polygonal cells arranged in irregular fascicles. The Masson trichrome staining showed modest amount of collagen supporting the neoplastic cells. PAS-positive content was not observed in the cytoplasm of neoplastic cells. Histological and histochemical findings indicated that it was a spindle cell neoplasm, but the classification was not possible. Immunohistochemistry was requested and performed using the streptavidin-biotin-peroxidase method. The markers used were anti-vimentin, anti-PCNA, anti-EMA, anti-melan A and anti-melanosome, antidesmin, anti-actin, anti-CD68 and anti- S100protein. The neoplastic cells were immunoreactive for vimentin and PCNA and negative for the other antibodies. The morphology characterization, histochemical and immunohistochemical analysis of neoplastic cells allowed the definitive diagnosis of oral fibrosarcoma.	
Palavras-Chave: 	Fibrossarcoma; Histochemical; Imuno-histoquímica; Neoplasma fusocelular; PCNA; Serpente; Vimentina.	
Thesagro: 	Histoquímica.	
Thesaurus Nal: 	Bothrops; Fibrosarcoma; Immunohistochemistry; Proliferating cell nuclear antigen; Snakes; Vimentin.	
Categoria do assunto: 	--	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/139060/1/Oral-fibrosarcoma.pdf	
Registro original: 	Embrapa Unidades Centrais (AI-SEDE)	
Exemplares: 	AI-SEDE - (58787-1) - UPE - AP - PP - 636.08905 - P474 -  - DIS