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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura; Embrapa Meio Ambiente. |
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Data corrente: |
07/06/2022 |
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Data da última atualização: |
08/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AMARAL, M. do; FREITAS, A. C. O.; SANTOS, A. S.; SANTOS, E. C. dos; FERREIRA, M. M.; GESTEIRA, A. da S.; GRAMACHO, K. P.; MARINHO-PRADO, J. S.; PIROVANI, C. P. |
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Afiliação: |
MILENA DO AMARAL, UESC; ANA CAMILA OLIVEIRA FREITAS, UESC; ARIANA SILVA SANTOS, UESC; EVERTON CRUZ DOS SANTOS, Instituto do Câncer José Alencar Gomes da Silva; MONALIZA MACÊDO FERREIRA, UESC; ABELMON DA SILVA GESTEIRA, CNPMF; KARINA PERES GRAMACHO, CEPEC; JEANNE SCARDINI MARINHO PRADO, CNPMA; CARLOS PRIMINHO PIROVANI, UESC. |
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Título: |
TcTI, a Kunitz-type trypsin inhibitor from cocoa associated with defense against pathogens. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Scientific Reports, v. 12, n. 1, article 680, 2022. |
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DOI: |
https://doi.org/10.1038/s41598-021-04700-y |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Protease inhibitors (PIs) are important biotechnological tools of interest in agriculture. Usually they are the first proteins to be activated in plant-induced resistance against pathogens. Therefore, the aim of this study was to characterize a Theobroma cacao trypsin inhibitor called TcTI. The ORF has 740 bp encoding a protein with 219 amino acids, molecular weight of approximately 23 kDa. rTcTI was expressed in the soluble fraction of Escherichia coli strain Rosetta [DE3]. The purified His-Tag rTcTI showed inhibitory activity against commercial porcine trypsin. The kinetic model demonstrated that rTcTI is a competitive inhibitor, with a Ki value of 4.08 × 10-7 mol L-1. The thermostability analysis of rTcTI showed that 100% inhibitory activity was retained up to 60 °C and that at 70-80 °C, inhibitory activity remained above 50%. Circular dichroism analysis indicated that the protein is rich in loop structures and beta-conformations. Furthermore, in vivo assays against Helicoverpa armigera larvae were also performed with rTcTI in 0.1 mg mL-1 spray solutions on leaf surfaces, which reduced larval growth by 70% compared to the control treatment. Trials with cocoa plants infected with Mp showed a greater accumulation of TcTI in resistant varieties of T. cacao, so this regulation may be associated with different isoforms of TcTI. This inhibitor has biochemical characteristics suitable for biotechnological applications as well as in resistance studies of T. cacao and other crops. MenosAbstract: Protease inhibitors (PIs) are important biotechnological tools of interest in agriculture. Usually they are the first proteins to be activated in plant-induced resistance against pathogens. Therefore, the aim of this study was to characterize a Theobroma cacao trypsin inhibitor called TcTI. The ORF has 740 bp encoding a protein with 219 amino acids, molecular weight of approximately 23 kDa. rTcTI was expressed in the soluble fraction of Escherichia coli strain Rosetta [DE3]. The purified His-Tag rTcTI showed inhibitory activity against commercial porcine trypsin. The kinetic model demonstrated that rTcTI is a competitive inhibitor, with a Ki value of 4.08 × 10-7 mol L-1. The thermostability analysis of rTcTI showed that 100% inhibitory activity was retained up to 60 °C and that at 70-80 °C, inhibitory activity remained above 50%. Circular dichroism analysis indicated that the protein is rich in loop structures and beta-conformations. Furthermore, in vivo assays against Helicoverpa armigera larvae were also performed with rTcTI in 0.1 mg mL-1 spray solutions on leaf surfaces, which reduced larval growth by 70% compared to the control treatment. Trials with cocoa plants infected with Mp showed a greater accumulation of TcTI in resistant varieties of T. cacao, so this regulation may be associated with different isoforms of TcTI. This inhibitor has biochemical characteristics suitable for biotechnological applications as well as in resistance studies of T. cacao and ot... Mostrar Tudo |
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Thesagro: |
Cacau; Inibidor de Enzima; Praga de Planta; Protease; Resistência; Theobroma Cacao. |
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Thesaurus Nal: |
Disease resistance; Helicoverpa armigera; Insecticide resistance; Trypsin inhibitors. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02552naa a2200349 a 4500
001 2143860
005 2022-06-08
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1038/s41598-021-04700-y$2DOI
100 1 $aAMARAL, M. do
245 $aTcTI, a Kunitz-type trypsin inhibitor from cocoa associated with defense against pathogens.$h[electronic resource]
260 $c2022
520 $aAbstract: Protease inhibitors (PIs) are important biotechnological tools of interest in agriculture. Usually they are the first proteins to be activated in plant-induced resistance against pathogens. Therefore, the aim of this study was to characterize a Theobroma cacao trypsin inhibitor called TcTI. The ORF has 740 bp encoding a protein with 219 amino acids, molecular weight of approximately 23 kDa. rTcTI was expressed in the soluble fraction of Escherichia coli strain Rosetta [DE3]. The purified His-Tag rTcTI showed inhibitory activity against commercial porcine trypsin. The kinetic model demonstrated that rTcTI is a competitive inhibitor, with a Ki value of 4.08 × 10-7 mol L-1. The thermostability analysis of rTcTI showed that 100% inhibitory activity was retained up to 60 °C and that at 70-80 °C, inhibitory activity remained above 50%. Circular dichroism analysis indicated that the protein is rich in loop structures and beta-conformations. Furthermore, in vivo assays against Helicoverpa armigera larvae were also performed with rTcTI in 0.1 mg mL-1 spray solutions on leaf surfaces, which reduced larval growth by 70% compared to the control treatment. Trials with cocoa plants infected with Mp showed a greater accumulation of TcTI in resistant varieties of T. cacao, so this regulation may be associated with different isoforms of TcTI. This inhibitor has biochemical characteristics suitable for biotechnological applications as well as in resistance studies of T. cacao and other crops.
650 $aDisease resistance
650 $aHelicoverpa armigera
650 $aInsecticide resistance
650 $aTrypsin inhibitors
650 $aCacau
650 $aInibidor de Enzima
650 $aPraga de Planta
650 $aProtease
650 $aResistência
650 $aTheobroma Cacao
700 1 $aFREITAS, A. C. O.
700 1 $aSANTOS, A. S.
700 1 $aSANTOS, E. C. dos
700 1 $aFERREIRA, M. M.
700 1 $aGESTEIRA, A. da S.
700 1 $aGRAMACHO, K. P.
700 1 $aMARINHO-PRADO, J. S.
700 1 $aPIROVANI, C. P.
773 $tScientific Reports$gv. 12, n. 1, article 680, 2022.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
28/02/2018 |
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Data da última atualização: |
28/02/2018 |
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Tipo da produção científica: |
Documentos |
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Autoria: |
SOUZA, F. de F.; DEON, M. D.; CASTRO, J. M. da C. e; CALGARO, M. |
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Afiliação: |
FLAVIO DE FRANCA SOUZA, CPATSA; MAGNUS DALLIGNA DEON, CPATSA; JOSE MAURO DA CUNHA E CASTRO, CPATSA; MARCELO CALGARO, CPATSA. |
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Título: |
Contribuições das pesquisas realizadas na Embrapa Semiárido para a cultura da aceroleira. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Petrolina: Embrapa Semiárido, 2017. |
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Páginas: |
26 p. |
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Série: |
(Embrapa Semiárido. Documentos, 282). |
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ISSN: |
1808-9992 |
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Idioma: |
Português |
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Conteúdo: |
Neste trabalho, são apresentados os resultados das contribuições da Embrapa Semiárido para a cultura da acerola. Inicialmente, faz-se um resgate sobre a introdução da acerola no Brasil e, em seguida, são apresentados resultados de pesquisas desenvolvidas pela Embrapa Semiárido, que vão do melhoramento à propagação e resistência a doenças. Por fim, destacam-se os principais entraves ao bom desempenho da atividade para a economia da região, apresentando-se algumas propostas que podem contribuir para a superação dos mesmos. |
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Palavras-Chave: |
Propagação; Semiárido. |
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Thesagro: |
Acerola; Doença; Fruticultura; Melhoramento genético vegetal; Variedade. |
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Thesaurus NAL: |
Malpighia emarginata. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/173370/1/SDC282.pdf
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Marc: |
LEADER 01338nam a2200277 a 4500
001 2088295
005 2018-02-28
008 2017 bl uuuu u0uu1 u #d
022 $a1808-9992
100 1 $aSOUZA, F. de F.
245 $aContribuições das pesquisas realizadas na Embrapa Semiárido para a cultura da aceroleira.$h[electronic resource]
260 $aPetrolina: Embrapa Semiárido$c2017
300 $a26 p.
490 $a(Embrapa Semiárido. Documentos, 282).
520 $aNeste trabalho, são apresentados os resultados das contribuições da Embrapa Semiárido para a cultura da acerola. Inicialmente, faz-se um resgate sobre a introdução da acerola no Brasil e, em seguida, são apresentados resultados de pesquisas desenvolvidas pela Embrapa Semiárido, que vão do melhoramento à propagação e resistência a doenças. Por fim, destacam-se os principais entraves ao bom desempenho da atividade para a economia da região, apresentando-se algumas propostas que podem contribuir para a superação dos mesmos.
650 $aMalpighia emarginata
650 $aAcerola
650 $aDoença
650 $aFruticultura
650 $aMelhoramento genético vegetal
650 $aVariedade
653 $aPropagação
653 $aSemiárido
700 1 $aDEON, M. D.
700 1 $aCASTRO, J. M. da C. e
700 1 $aCALGARO, M.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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