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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Algodão; Embrapa Semiárido. |
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Data corrente: |
05/09/2022 |
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Data da última atualização: |
04/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
JOVINO, R. S.; SILVA, T. R. da; RODRIGUES, R. T.; CARVALHO, J. R. de S.; CUNHA, J. B. de A.; LIMA, L. M. de; SANTOS, R. C. dos; SANTOS, C. E. de R. e S.; RIBEIRO, P. R. de A.; FREITAS, A. D. S. de; MARTINS, L. M. V.; FERNANDES JUNIOR, P. I. |
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Afiliação: |
RAPHAEL SILVA JOVINO, Universidade Estadual da Paraíba; THAÍSE ROSA DA SILVA, UNIVASF; RUTH TEREZINHA RODRIGUES, UNIVASF; JACKSON RAFAEL DE SÁ CARVALHO, Universidade Estadual de Feira de Santana; JUSSARA BARBOZA DE ALENCAR CUNHA, UNEB/DTCS; LIZIANE MARIA DE LIMA, CNPA; ROSEANE CAVALCANTI DOS SANTOS, CNPA; CAROLINA ETIENNE DE ROSÁLIA E SILVA SANTOS, UFRPE; PAULA ROSE DE ALMEIDA RIBEIRO, Bolsista CNPq; ANA DOLORES SANTIAGO DE FREITAS, UFRPE; LINDETE MÍRIA VIEIRA MARTINS, UNEB/DTCS; PAULO IVAN FERNANDES JUNIOR, CPATSA. |
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Título: |
Elite Bradyrhizobium strains boost biological nitrogen fixation and peanut yield in tropical drylands. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 53, p. 1623-1632, 2022. |
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DOI: |
https://doi.org/10.1007/s42770-022-00792-4 |
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Idioma: |
Inglês |
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Conteúdo: |
Peanut (Arachis hypogaea L.) is an important crop for the family-based systems in the tropics, mainly in Brazil. In the Brazilian drylands, peanuts are cropped in low technological systems, and cheap and efficient technologies are needed to improve crop yield and sustainability. Despite this importance, few data are available on selecting efficient peanut rhizobia in experiments under different edaphoclimatic conditions. This work evaluated the agronomic efficiency and the biological nitrogen fixation (BNF) by two elite Bradyrhizobium strains under four different fields in the Brazilian semiarid region. We compared a new efficient strain Bradyrhizobium sp. ESA 123 with the reference strain B. elkanii SEMIA 6144, currently used in peanut rhizobial inoculants in Brazil. Besides the inoculated treatments, two uninoculated controls were assessed (with and without 80 kg ha−1 of N-urea). The BNF was estimated by the δ15N approach in three out of four field assays. BNF contribution was improved by inoculation of both Bradyrhizobium strains, ranging from 42 to 51% in Petrolina and 43 to 60% in Nossa Senhora da Glória. Peanuts? yields benefited from the inoculation of both strains and N fertilization in all four assays. Nevertheless, the results showed the efficiency of both strains under different edaphoclimatic conditions, indicating the native strain ESA 123 as a potential bacterium for recommendation as inoculants for peanuts in Brazil, mainly in drylands. |
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Palavras-Chave: |
Elite Bradyrhizobium; FBN; Inoculação de sementes; Rhizobia; Seleção de cepas. |
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Thesagro: |
Amendoim; Arachis Hypogaea; Fixação de Nitrogênio; Inoculante. |
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Thesaurus Nal: |
Arachis hypogaea subsp. hypogaea; Peanuts; Rhizobiaceae; Seed inoculation. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02772naa a2200421 a 4500
001 2151488
005 2023-02-04
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s42770-022-00792-4$2DOI
100 1 $aJOVINO, R. S.
245 $aElite Bradyrhizobium strains boost biological nitrogen fixation and peanut yield in tropical drylands.$h[electronic resource]
260 $c2022
520 $aPeanut (Arachis hypogaea L.) is an important crop for the family-based systems in the tropics, mainly in Brazil. In the Brazilian drylands, peanuts are cropped in low technological systems, and cheap and efficient technologies are needed to improve crop yield and sustainability. Despite this importance, few data are available on selecting efficient peanut rhizobia in experiments under different edaphoclimatic conditions. This work evaluated the agronomic efficiency and the biological nitrogen fixation (BNF) by two elite Bradyrhizobium strains under four different fields in the Brazilian semiarid region. We compared a new efficient strain Bradyrhizobium sp. ESA 123 with the reference strain B. elkanii SEMIA 6144, currently used in peanut rhizobial inoculants in Brazil. Besides the inoculated treatments, two uninoculated controls were assessed (with and without 80 kg ha−1 of N-urea). The BNF was estimated by the δ15N approach in three out of four field assays. BNF contribution was improved by inoculation of both Bradyrhizobium strains, ranging from 42 to 51% in Petrolina and 43 to 60% in Nossa Senhora da Glória. Peanuts? yields benefited from the inoculation of both strains and N fertilization in all four assays. Nevertheless, the results showed the efficiency of both strains under different edaphoclimatic conditions, indicating the native strain ESA 123 as a potential bacterium for recommendation as inoculants for peanuts in Brazil, mainly in drylands.
650 $aArachis hypogaea subsp. hypogaea
650 $aPeanuts
650 $aRhizobiaceae
650 $aSeed inoculation
650 $aAmendoim
650 $aArachis Hypogaea
650 $aFixação de Nitrogênio
650 $aInoculante
653 $aElite Bradyrhizobium
653 $aFBN
653 $aInoculação de sementes
653 $aRhizobia
653 $aSeleção de cepas
700 1 $aSILVA, T. R. da
700 1 $aRODRIGUES, R. T.
700 1 $aCARVALHO, J. R. de S.
700 1 $aCUNHA, J. B. de A.
700 1 $aLIMA, L. M. de
700 1 $aSANTOS, R. C. dos
700 1 $aSANTOS, C. E. de R. e S.
700 1 $aRIBEIRO, P. R. de A.
700 1 $aFREITAS, A. D. S. de
700 1 $aMARTINS, L. M. V.
700 1 $aFERNANDES JUNIOR, P. I.
773 $tBrazilian Journal of Microbiology$gv. 53, p. 1623-1632, 2022.
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Registro original: |
Embrapa Algodão (CNPA) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
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URL |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
31/03/1993 |
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Data da última atualização: |
05/03/2024 |
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Autoria: |
KITAJIMA, E. W.; RESENDE, R. de O.; AVILA, A. C. de; GOLDBACH, R. W.; PETERS, D. |
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Afiliação: |
ANTONIO CARLOS DE AVILA, CNPH. |
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Título: |
Immuno-electron microscopical detection of tomato spotted wilt virus and its nucleocapsids in crude plant extracts. |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
Journal of Virological Methods, v. 38, n. 3, p. 313-322, Aug./Sept. 1992. |
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DOI: |
https://doi.org/10.1016/0166-0934(92)90076-P |
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Idioma: |
Inglês |
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Conteúdo: |
Tomato spotted wilt virus (TSWV) particles were identified in thin sections and in crude extracts of leaves from plants infected with different TSWV isolates, using gold labelled protein A and antibodies prepared against purified virus particles or against nucleocapsid preparations. In addition, both in thin sections and in dip preparations aggregates were detected using either goldlabelled antiserum. These aggregates were not detected using antisera against envelope proteins or against the non-structural protein NSs. They were the only detectably labelled material in preparations made from plants infected by morphologically defective isolates of TSWV, i.e. isolates that did not produce enveloped particles. The aggregates were discerned in dip preparations as cloudy amorphous structures having a cottonwool boll-like appearance. These ‘cotton bolls’ were interpreted as being the in vitro structures of the nucleocapsid aggregates occurring in plant cells infected by isolates maintained by mechanical inoculation and are the only structures in cells infected by morphologically defective isolates (Ie, 1982; Kitajima et al., 1992). The detection of the ‘cotton boll-like’ structures in crude extracts provides an adequate and rapid method to establish the presence of nucleocapsid aggregates in cells of infected plants, irrespective of whether they are infected by morphologically defective isolates or not. |
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Palavras-Chave: |
Deteccao; Immunogold; Imunoflorescencia; Labelling; TSWV; Vira-Cabeca. |
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Thesaurus NAL: |
detection; Tomato spotted wilt virus. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02285naa a2200277 a 4500
001 1749564
005 2024-03-05
008 1992 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/0166-0934(92)90076-P$2DOI
100 1 $aKITAJIMA, E. W.
245 $aImmuno-electron microscopical detection of tomato spotted wilt virus and its nucleocapsids in crude plant extracts.$h[electronic resource]
260 $c1992
520 $aTomato spotted wilt virus (TSWV) particles were identified in thin sections and in crude extracts of leaves from plants infected with different TSWV isolates, using gold labelled protein A and antibodies prepared against purified virus particles or against nucleocapsid preparations. In addition, both in thin sections and in dip preparations aggregates were detected using either goldlabelled antiserum. These aggregates were not detected using antisera against envelope proteins or against the non-structural protein NSs. They were the only detectably labelled material in preparations made from plants infected by morphologically defective isolates of TSWV, i.e. isolates that did not produce enveloped particles. The aggregates were discerned in dip preparations as cloudy amorphous structures having a cottonwool boll-like appearance. These ‘cotton bolls’ were interpreted as being the in vitro structures of the nucleocapsid aggregates occurring in plant cells infected by isolates maintained by mechanical inoculation and are the only structures in cells infected by morphologically defective isolates (Ie, 1982; Kitajima et al., 1992). The detection of the ‘cotton boll-like’ structures in crude extracts provides an adequate and rapid method to establish the presence of nucleocapsid aggregates in cells of infected plants, irrespective of whether they are infected by morphologically defective isolates or not.
650 $adetection
650 $aTomato spotted wilt virus
653 $aDeteccao
653 $aImmunogold
653 $aImunoflorescencia
653 $aLabelling
653 $aTSWV
653 $aVira-Cabeca
700 1 $aRESENDE, R. de O.
700 1 $aAVILA, A. C. de
700 1 $aGOLDBACH, R. W.
700 1 $aPETERS, D.
773 $tJournal of Virological Methods$gv. 38, n. 3, p. 313-322, Aug./Sept. 1992.
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