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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
07/01/2016 |
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Data da última atualização: |
03/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PASSOS, L. P.; SANTOS, A. S. de O. dos; MARTINS, M. F.; BOTEZINE, N. P.; LOPES, H. C. da C.; DIAS, D. H. da S.; FOGAÇA, G. N.; REIS, P. R. C.; MARQUES, R. |
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Afiliação: |
LEONIDAS PAIXAO PASSOS, CNPGL; Alessa Siqueira de Oliveira dos Santos; MARTA FONSECA MARTINS, CNPGL; Naiara Pereira Botezine; Heitor Carvalho da Costa Lopes; Diego Henrique da Silva Dias; Gisele Nogueira Fogaça; Paola Ramos Coutinho Reis; Rafael Marques. |
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Título: |
Response of iron-enriched palisadegrass seedlings to toxic Cr3+ revealed by physiological indicators and protein profiles using microfluidic electrophoresis. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Australian Journal of Crop Science, v. 9, n. 9, p. 835-843, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Most studies on crop responses to Cr toxicity lack using acidic growing medium in order to maximize Cr uptake and taking measures to prevent Cr-induced Fe deficiency. Also, the evaluation of protein profiles is still to be carried out in association with physiological responses to Cr stress. The purpose of this study was to investigate the effects of Cr3+ on the physiology and protein profiles of Feenriched palisadegrass seedlings in an acidic nutrient solution under controlled conditions. Following 30 days of continuous stress, a sharp reduction in leaf area was the most evident alteration caused by Cr3+ stress, while a tendency of depressing effects was observed in the other physiological variables. Nutrient solution acidity had no significant effect, which could be associated with the high tolerance of palisadegrass to acidic soils. Symptoms of Fe deficiency in Cr3+ -treated seedlings were minimal, suggesting Feenrichment was efficient in isolating such a stress from Cr3+ effects. Proteins were differentially expressed in Cr3+ -treated leaf samples, as revealed by microfluidic electrophoresis and SDS-PAGE profiles. In both cases, protein spots were less noticeable under Cr3+ stress. It is concluded that a depressed leaf area is the most rapid and detectable response of the species to Cr3+ toxicity, along with an overall down-regulation of protein spots. Other variables, such as number of leaves, number of dead leaves, shoot length and chlorophyll level are putative indicators for a prolonged exposure to Cr3+. MenosMost studies on crop responses to Cr toxicity lack using acidic growing medium in order to maximize Cr uptake and taking measures to prevent Cr-induced Fe deficiency. Also, the evaluation of protein profiles is still to be carried out in association with physiological responses to Cr stress. The purpose of this study was to investigate the effects of Cr3+ on the physiology and protein profiles of Feenriched palisadegrass seedlings in an acidic nutrient solution under controlled conditions. Following 30 days of continuous stress, a sharp reduction in leaf area was the most evident alteration caused by Cr3+ stress, while a tendency of depressing effects was observed in the other physiological variables. Nutrient solution acidity had no significant effect, which could be associated with the high tolerance of palisadegrass to acidic soils. Symptoms of Fe deficiency in Cr3+ -treated seedlings were minimal, suggesting Feenrichment was efficient in isolating such a stress from Cr3+ effects. Proteins were differentially expressed in Cr3+ -treated leaf samples, as revealed by microfluidic electrophoresis and SDS-PAGE profiles. In both cases, protein spots were less noticeable under Cr3+ stress. It is concluded that a depressed leaf area is the most rapid and detectable response of the species to Cr3+ toxicity, along with an overall down-regulation of protein spots. Other variables, such as number of leaves, number of dead leaves, shoot length and chlorophyll level are putative indica... Mostrar Tudo |
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Palavras-Chave: |
Cr3+; Iron supply; Medium pH; Microfluidic electrophoresis; Palisadegrass; Physiological indicator. |
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Thesagro: |
Brachiaria Brizantha. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/136770/1/Cnpgl-2015-AustrJCropS-Response.pdf
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Marc: |
LEADER 02495naa a2200301 a 4500
001 2033161
005 2024-02-03
008 2015 bl uuuu u00u1 u #d
100 1 $aPASSOS, L. P.
245 $aResponse of iron-enriched palisadegrass seedlings to toxic Cr3+ revealed by physiological indicators and protein profiles using microfluidic electrophoresis.$h[electronic resource]
260 $c2015
520 $aMost studies on crop responses to Cr toxicity lack using acidic growing medium in order to maximize Cr uptake and taking measures to prevent Cr-induced Fe deficiency. Also, the evaluation of protein profiles is still to be carried out in association with physiological responses to Cr stress. The purpose of this study was to investigate the effects of Cr3+ on the physiology and protein profiles of Feenriched palisadegrass seedlings in an acidic nutrient solution under controlled conditions. Following 30 days of continuous stress, a sharp reduction in leaf area was the most evident alteration caused by Cr3+ stress, while a tendency of depressing effects was observed in the other physiological variables. Nutrient solution acidity had no significant effect, which could be associated with the high tolerance of palisadegrass to acidic soils. Symptoms of Fe deficiency in Cr3+ -treated seedlings were minimal, suggesting Feenrichment was efficient in isolating such a stress from Cr3+ effects. Proteins were differentially expressed in Cr3+ -treated leaf samples, as revealed by microfluidic electrophoresis and SDS-PAGE profiles. In both cases, protein spots were less noticeable under Cr3+ stress. It is concluded that a depressed leaf area is the most rapid and detectable response of the species to Cr3+ toxicity, along with an overall down-regulation of protein spots. Other variables, such as number of leaves, number of dead leaves, shoot length and chlorophyll level are putative indicators for a prolonged exposure to Cr3+.
650 $aBrachiaria Brizantha
653 $aCr3+
653 $aIron supply
653 $aMedium pH
653 $aMicrofluidic electrophoresis
653 $aPalisadegrass
653 $aPhysiological indicator
700 1 $aSANTOS, A. S. de O. dos
700 1 $aMARTINS, M. F.
700 1 $aBOTEZINE, N. P.
700 1 $aLOPES, H. C. da C.
700 1 $aDIAS, D. H. da S.
700 1 $aFOGAÇA, G. N.
700 1 $aREIS, P. R. C.
700 1 $aMARQUES, R.
773 $tAustralian Journal of Crop Science$gv. 9, n. 9, p. 835-843, 2015.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Hortaliças. |
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Data corrente: |
09/08/2022 |
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Data da última atualização: |
11/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
MARTINS, T. P.; REGO, C. M.; NAKASU, E. Y. T.; FERNANDES, F. R.; INOUE-NAGATA, A. K. |
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Afiliação: |
T. P. MARTINS, UNIVERSIDADE DE BRASÍLIA; C. M. REGO, UNIVERSIDADE DE BRASÍLIA; ERICH YUKIO TEMPEL NAKASU, CNPH; FERNANDA RAUSCH FERNANDES, CNPTIA; ALICE KAZUKO INOUE NAGATA, CNPH. |
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Título: |
A high viral diversity in tomato crops in Brazil is revealed by next generation sequencing analyses. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Acta Horticulturae, v. 1316, p. 99-105, 2021. |
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ISSN: |
2406-6168 |
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DOI: |
10.17660/ActaHortic.2021.1316.14 |
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Idioma: |
Inglês |
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Notas: |
Edition of Proceedings of the VI International Symposium on Tomato Diseases: Managing Tomato Diseases in the Face of Globalization and Climate Change. |
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Conteúdo: |
Tomato is planted in Brazil mainly for fresh consumption and tomato paste production. Among the various pathogens that infect tomato plants in Brazil, viruses are particularly important due to their high incidence and the resulting losses caused. Diagnosis of viral diseases usually relies on detection methods directed to known viruses and close variants, either by serology or nucleic acid hybridization/ amplification. However, the advent of next generation sequencing (NGS) facilitated a deep analysis of viral populations, which can be used for identification, assembly and discovery of new viruses. Aiming to estimate the viral diversity present in tomato crops from three states of Brazil, five composite leaf samples were analyzed using NGS. The samples referred as Braz (collected in the Federal District, 2015); Ahol, Toca1, and Toca2 (São Paulo State, 2014), and RNY2 (Minas Gerais State, 2013) were submitted to semi-purification of viral particles and RNA extraction before RNA-seq (Illumina). The reads were filtered for quality, the contigs assembled (Velvet algorithm), and submitted to MegaBLAST analysis against a virus reference sequences database. These samples were collected from plants showing symptoms such as mosaic, chlorosis, leaf curling, chlorotic spots, necrosis and stunting. Known viruses belonging to nine genera, Crinivirus, Begomovirus, Tospovirus, Tobravirus, Potyvirus, Tobamovirus, Tymovirus, Potexvirus and Cucumovirus, were detected. Potentially undescribed and unreported viruses in tomatoes, such as an amalgavirus and an ilarvirus, were also detected and are under confirmation. The conclusion was that there is a high virus diversity present in tomato plants in Brazil, making tomato production a challenge to the growers. The crinivirus, Tomato chlorosis virus, was the most frequently found within the samplings, suggesting that it is widespread in the major tomato production areas. Two begomoviruses were detected, implying that this strategy is also useful to detect viruses with a DNA genome. Finally, this technique was particularly convenient to identify the viruses coexisting in tomatoes and to find unknown viruses that may threaten the tomato production in the country. MenosTomato is planted in Brazil mainly for fresh consumption and tomato paste production. Among the various pathogens that infect tomato plants in Brazil, viruses are particularly important due to their high incidence and the resulting losses caused. Diagnosis of viral diseases usually relies on detection methods directed to known viruses and close variants, either by serology or nucleic acid hybridization/ amplification. However, the advent of next generation sequencing (NGS) facilitated a deep analysis of viral populations, which can be used for identification, assembly and discovery of new viruses. Aiming to estimate the viral diversity present in tomato crops from three states of Brazil, five composite leaf samples were analyzed using NGS. The samples referred as Braz (collected in the Federal District, 2015); Ahol, Toca1, and Toca2 (São Paulo State, 2014), and RNY2 (Minas Gerais State, 2013) were submitted to semi-purification of viral particles and RNA extraction before RNA-seq (Illumina). The reads were filtered for quality, the contigs assembled (Velvet algorithm), and submitted to MegaBLAST analysis against a virus reference sequences database. These samples were collected from plants showing symptoms such as mosaic, chlorosis, leaf curling, chlorotic spots, necrosis and stunting. Known viruses belonging to nine genera, Crinivirus, Begomovirus, Tospovirus, Tobravirus, Potyvirus, Tobamovirus, Tymovirus, Potexvirus and Cucumovirus, were detected. Potentially undescribed a... Mostrar Tudo |
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Palavras-Chave: |
HTS; Next Generation Sequencing; Sequenciamento de nova geração; Virome. |
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Thesagro: |
Tomate; Vírus. |
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Thesaurus NAL: |
Solanum lycopersicum. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03214naa a2200289 a 4500
001 2145363
005 2022-08-11
008 2021 bl uuuu u00u1 u #d
022 $a2406-6168
024 7 $a10.17660/ActaHortic.2021.1316.14$2DOI
100 1 $aMARTINS, T. P.
245 $aA high viral diversity in tomato crops in Brazil is revealed by next generation sequencing analyses.$h[electronic resource]
260 $c2021
500 $aEdition of Proceedings of the VI International Symposium on Tomato Diseases: Managing Tomato Diseases in the Face of Globalization and Climate Change.
520 $aTomato is planted in Brazil mainly for fresh consumption and tomato paste production. Among the various pathogens that infect tomato plants in Brazil, viruses are particularly important due to their high incidence and the resulting losses caused. Diagnosis of viral diseases usually relies on detection methods directed to known viruses and close variants, either by serology or nucleic acid hybridization/ amplification. However, the advent of next generation sequencing (NGS) facilitated a deep analysis of viral populations, which can be used for identification, assembly and discovery of new viruses. Aiming to estimate the viral diversity present in tomato crops from three states of Brazil, five composite leaf samples were analyzed using NGS. The samples referred as Braz (collected in the Federal District, 2015); Ahol, Toca1, and Toca2 (São Paulo State, 2014), and RNY2 (Minas Gerais State, 2013) were submitted to semi-purification of viral particles and RNA extraction before RNA-seq (Illumina). The reads were filtered for quality, the contigs assembled (Velvet algorithm), and submitted to MegaBLAST analysis against a virus reference sequences database. These samples were collected from plants showing symptoms such as mosaic, chlorosis, leaf curling, chlorotic spots, necrosis and stunting. Known viruses belonging to nine genera, Crinivirus, Begomovirus, Tospovirus, Tobravirus, Potyvirus, Tobamovirus, Tymovirus, Potexvirus and Cucumovirus, were detected. Potentially undescribed and unreported viruses in tomatoes, such as an amalgavirus and an ilarvirus, were also detected and are under confirmation. The conclusion was that there is a high virus diversity present in tomato plants in Brazil, making tomato production a challenge to the growers. The crinivirus, Tomato chlorosis virus, was the most frequently found within the samplings, suggesting that it is widespread in the major tomato production areas. Two begomoviruses were detected, implying that this strategy is also useful to detect viruses with a DNA genome. Finally, this technique was particularly convenient to identify the viruses coexisting in tomatoes and to find unknown viruses that may threaten the tomato production in the country.
650 $aSolanum lycopersicum
650 $aTomate
650 $aVírus
653 $aHTS
653 $aNext Generation Sequencing
653 $aSequenciamento de nova geração
653 $aVirome
700 1 $aREGO, C. M.
700 1 $aNAKASU, E. Y. T.
700 1 $aFERNANDES, F. R.
700 1 $aINOUE-NAGATA, A. K.
773 $tActa Horticulturae$gv. 1316, p. 99-105, 2021.
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