Data/hora: 10/06/2024 03:31:28

Biblioteca(s): | Embrapa Arroz e Feijão.
Data corrente: | 21/10/2004
Data da última atualização: | 26/10/2004
Autoria: | SILVEIRA, V.; STEINER, N.; SANTOS, A. L. W. dos; NODARI, R. O.; GUERRA, M. P.
Título: | Biotechnology tolls in Araucaria angustifolia conservation and improvement: inductive factors affecting somatic embryogenesis.
Ano de publicação: | 2002
Fonte/Imprenta: | Crop Breeding and Applied Biotechnology, Londrina, v. 2, n. 3, p. 463-470, Sept. 2002.
Idioma: | Inglês
Conteúdo: | Biotechnological techniques based on somatic embryogenesis have been employed for capturing genetic gains in forest breeding  programs. The present work aimed at studying the factors associated with the induction of somatic embryogenesis in Araucaria angustifolia. Zygotic embryos in different development stages were inoculated in a LP medium supplemented with 2,4-D, BA and KIN. Embryogenic cultures were inoculated in a LP liquid medium free of plant growth regulators. ABA and/or PEG 4000 were evaluated for the development of somatic embryos. The embryogenic induction was dependent on the genotype of the mother plant, ranging from 21.9% to 55.2%. Suspension cultures showed a 5.6 times increment of the initial cell volume after 33 days in the culture medium free of plant growth regulators. It was possible to establish and multiply autonomous embryogenic cultures. The medium containing ABA 50 (mM) and PEG (1%) resulted in the development o globular and torpedo somatic embryos.
Palavras-Chave: | Autonomous; Brazilian pine; Conservation; Embriogênese somática; Improvement; Somatic; Suspension cultures.
Thesagro: | Araucária Angustifólia; Melhoramento.
Thesaurus Nal: | embryogenesis.
Categoria do assunto: | --
Registro original: | Embrapa Arroz e Feijão (CNPAF)
Exemplares: | CNPAF - (21752-1) - ADD - AP - -- - 631.05 - C - 2004.00112 - DIS