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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
09/04/2010 |
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Data da última atualização: |
25/09/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FONSECA, J. F. da; TORRES, C. A. A.; MAFFILI, V. V.; BORGES, A. M.; SANTOS, A. D. F.; RODRIGUES, M. T.; OLIVEIRA, R. F. M. |
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Afiliação: |
JEFERSON FERREIRA DA FONSECA, CNPC; UFV, Viçosa, MG; UFV; UFV; UFV; UFV; UFV. |
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Título: |
The hypoosmotic swelling test in fresh goat spermatozoa. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Animal Reproduction, v. 2, n. 2, p. 139-144, Apr./Jun. 2005. |
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Idioma: |
Inglês |
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Conteúdo: |
The hypoosmotic swelling test (HOST) has been proved to be a good tool for evaluating the membrane integrity of spermatozoa of various domestic animals including cattle, horses, and swine. However, the best approach for using this technique in goat semen has not been tested. The present study aimed to establish the best hypoosmotic solution (HS) for testing membrane integrity in fresh goat semen. Sodium citrate and fructose based solutions (S) with the following osmolarities (mOsm/l) were used: 50 (S1), 75 (S2), 100 (S3), 125 (S4), 150 (S5), 175 (S6), 200 (S7), 250 (S8), 290 (S9) and 300 mOsm/l (S10). Twenty-eight semen samples were collected from seven mature bucks (four collections per buck) at 48 hour intervals. After macroscopic evaluation, 10?l of semen was immediately added to 2ml of each solution and incubated for one hour at 37ºC. Sequentially, 20?l of semen diluted in HS were evaluated with oil immersion using a phase-contrast microscope. A total of 200 spermatozoa were counted in at least five different fields, and sperm tails were classified as non-coiled, coiled, and strongly coiled. The respective percentages of spermatozoa with coiled tails (coiled plus strongly coiled) in the ten solutions listed above were 34.1, 38.8, 45.3, 51.5, 46.8, 42.8, 38.2, 29.0, 19.4 and 23.1%. Percentages of strongly coiled spermatozoa were: 6.8, 10.6, 21.5, 25.3, 24.3, 21.5, 19.3, 12.4, 6.4, and 7.9 for the ten solutions, respectively. According to total coiling, S4 was superior to S1, S7, S8, S9, and S10 (P<0.05). According to strong coiling, S4 was superior to S1, S2, S8, S9, and S10 (P<0.05). Results suggest that the 125 mOsm/l solution would be best for use in HOST in fresh goat spermatozoa. MenosThe hypoosmotic swelling test (HOST) has been proved to be a good tool for evaluating the membrane integrity of spermatozoa of various domestic animals including cattle, horses, and swine. However, the best approach for using this technique in goat semen has not been tested. The present study aimed to establish the best hypoosmotic solution (HS) for testing membrane integrity in fresh goat semen. Sodium citrate and fructose based solutions (S) with the following osmolarities (mOsm/l) were used: 50 (S1), 75 (S2), 100 (S3), 125 (S4), 150 (S5), 175 (S6), 200 (S7), 250 (S8), 290 (S9) and 300 mOsm/l (S10). Twenty-eight semen samples were collected from seven mature bucks (four collections per buck) at 48 hour intervals. After macroscopic evaluation, 10?l of semen was immediately added to 2ml of each solution and incubated for one hour at 37ºC. Sequentially, 20?l of semen diluted in HS were evaluated with oil immersion using a phase-contrast microscope. A total of 200 spermatozoa were counted in at least five different fields, and sperm tails were classified as non-coiled, coiled, and strongly coiled. The respective percentages of spermatozoa with coiled tails (coiled plus strongly coiled) in the ten solutions listed above were 34.1, 38.8, 45.3, 51.5, 46.8, 42.8, 38.2, 29.0, 19.4 and 23.1%. Percentages of strongly coiled spermatozoa were: 6.8, 10.6, 21.5, 25.3, 24.3, 21.5, 19.3, 12.4, 6.4, and 7.9 for the ten solutions, respectively. According to total coiling, S4 was superior to ... Mostrar Tudo |
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Palavras-Chave: |
Goat; Hypoosmotic swelling test; Sperm. |
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Thesagro: |
Caprino; Congelamento; Reprodução animal; Sêmen. |
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Thesaurus Nal: |
Animal reproduction; Tail. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/75517/1/API-The-hypoosmotic.pdf
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Marc: |
LEADER 02537naa a2200301 a 4500
001 1663713
005 2019-09-25
008 2005 bl uuuu u00u1 u #d
100 1 $aFONSECA, J. F. da
245 $aThe hypoosmotic swelling test in fresh goat spermatozoa.$h[electronic resource]
260 $c2005
520 $aThe hypoosmotic swelling test (HOST) has been proved to be a good tool for evaluating the membrane integrity of spermatozoa of various domestic animals including cattle, horses, and swine. However, the best approach for using this technique in goat semen has not been tested. The present study aimed to establish the best hypoosmotic solution (HS) for testing membrane integrity in fresh goat semen. Sodium citrate and fructose based solutions (S) with the following osmolarities (mOsm/l) were used: 50 (S1), 75 (S2), 100 (S3), 125 (S4), 150 (S5), 175 (S6), 200 (S7), 250 (S8), 290 (S9) and 300 mOsm/l (S10). Twenty-eight semen samples were collected from seven mature bucks (four collections per buck) at 48 hour intervals. After macroscopic evaluation, 10?l of semen was immediately added to 2ml of each solution and incubated for one hour at 37ºC. Sequentially, 20?l of semen diluted in HS were evaluated with oil immersion using a phase-contrast microscope. A total of 200 spermatozoa were counted in at least five different fields, and sperm tails were classified as non-coiled, coiled, and strongly coiled. The respective percentages of spermatozoa with coiled tails (coiled plus strongly coiled) in the ten solutions listed above were 34.1, 38.8, 45.3, 51.5, 46.8, 42.8, 38.2, 29.0, 19.4 and 23.1%. Percentages of strongly coiled spermatozoa were: 6.8, 10.6, 21.5, 25.3, 24.3, 21.5, 19.3, 12.4, 6.4, and 7.9 for the ten solutions, respectively. According to total coiling, S4 was superior to S1, S7, S8, S9, and S10 (P<0.05). According to strong coiling, S4 was superior to S1, S2, S8, S9, and S10 (P<0.05). Results suggest that the 125 mOsm/l solution would be best for use in HOST in fresh goat spermatozoa.
650 $aAnimal reproduction
650 $aTail
650 $aCaprino
650 $aCongelamento
650 $aReprodução animal
650 $aSêmen
653 $aGoat
653 $aHypoosmotic swelling test
653 $aSperm
700 1 $aTORRES, C. A. A.
700 1 $aMAFFILI, V. V.
700 1 $aBORGES, A. M.
700 1 $aSANTOS, A. D. F.
700 1 $aRODRIGUES, M. T.
700 1 $aOLIVEIRA, R. F. M.
773 $tAnimal Reproduction$gv. 2, n. 2, p. 139-144, Apr./Jun. 2005.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria de Alimentos. Para informações adicionais entre em contato com ctaa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
07/03/2012 |
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Data da última atualização: |
07/03/2012 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BARRABIN, J. S.; BORGUINI, R. G.; GODOY, R. L. O.; ROSA, J. S. da; SCHULZ, D. F. |
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Afiliação: |
JULIANA S. BARRABIN, UFRRJ; RENATA GALHARDO BORGUINI, CTAA; RONOEL LUIZ DE OLIVEIRA GODOY, CTAA; JEANE SANTOS DA ROSA, CTAA; DANIEL F. SCHULZ, UFRJ. |
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Título: |
Preparation of calibration curves for UPLC-ESI-QTOF-MS determination of aflatoxinas B1, B2, G1, and G2. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: ISM CONFERENCE, 2011, Mendoza. Strategies to reduce the impact of mycotoxins in Latin America in a global context: book of abstracts. Río Cuarto: Universidad Nacional de Río Cuarto, 2011. p. 233. |
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Idioma: |
Inglês |
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Palavras-Chave: |
Aflatoxinas; Espectrometria de massa; Micotoxinas. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 00734naa a2200193 a 4500
001 1917840
005 2012-03-07
008 2011 bl --- 0-- u #d
100 1 $aBARRABIN, J. S.
245 $aPreparation of calibration curves for UPLC-ESI-QTOF-MS determination of aflatoxinas B1, B2, G1, and G2.
260 $c2011
653 $aAflatoxinas
653 $aEspectrometria de massa
653 $aMicotoxinas
700 1 $aBORGUINI, R. G.
700 1 $aGODOY, R. L. O.
700 1 $aROSA, J. S. da
700 1 $aSCHULZ, D. F.
773 $tIn: ISM CONFERENCE, 2011, Mendoza. Strategies to reduce the impact of mycotoxins in Latin America in a global context: book of abstracts. Río Cuarto: Universidad Nacional de Río Cuarto, 2011. p. 233.
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Embrapa Agroindústria de Alimentos (CTAA) |
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