Data/hora: 08/06/2024 06:34:06

Biblioteca(s): 	Embrapa Meio-Norte.	
Data corrente: 	06/12/2012	
Data da última atualização: 	01/06/2022	
Autoria: 	CASTRO, P. F.; FREITAS JUNIOR, A. C. V.; SANTANA, W. M.; COSTA, H. M. S.; CARVALHO JUNIOR, L. B.; BEZERRA, R. S.	
Afiliação: 	PATRICIA FERNANDES DE CASTRO, CPAMN; AUGUSTO C. V. FREITAS JUNIOR, UFPE; WERLAYNE M. SANTANA, UFPE; HELANE M. S. COSTA, UFPE; LUIZ B. CARVALHO JUNIOR, UFPE; RANILSON S. BEZERRA, UFPE.	
Título: 	Comparative study of amylases from the midgut gland of three species of penaeid shrimp.	
Ano de publicação: 	2012	
Fonte/Imprenta: 	Journal of Crustacean Biology, v. 32, n. 4, p. 607-613, 2012.	
Idioma: 	Inglês	
Conteúdo: 	Amylases from the midgut gland of wild Farfantepenaeus subtilis (Pérez-Farfante, 1967) and Litopenaeus schmitti (Burkenroad, 1936), and farmed Litopenaeus vannamei (Boone, 1931) were characterized through studies on the effect of inhibitor and metallic ions, optimal pH and temperature, thermal stability and zymograms. The substrate zymogram revealed nine, eight, ten and seven amylolytic bands from F. subtilis, L. schmitti, adults and juveniles L. vannamei, respectively. Total amylolytic activity in the farmed shrimp was three times as high as that of the wild specimens. Amylases from all species exhibited residual activity above 85% at alkaline pH (7.0-8.0), with optimal temperature between 40 and 50°C. None of the enzymes from the species were thermally stable at temperatures above 55°C. Alpha-amylase activity in F. subtilis and L. schmitti was totally inhibited by Type I inhibitor at 50 and 100 g.mL-1, while enzymes from adult and juvenile L. vannamei retained 43 5 ± 1 98 and 22 5 ± 0 65% of their activity, respectively, at these same concentrations. Ca2+ increased amylase activity in all species only at a concentration of 1 mM, inhibiting activity at 5 and 10 mM. All other ions employed (Cd2+,Zn2+,Hg2+,Cu2+ and Al 3+) strongly inhibited amylase activity, regardless of the concentration used.	
Palavras-Chave: 	Caracterização amilase; Glândula do intestino médio.	
Thesaurus Nal: 	Farfantepenaeus subtilis; Litopenaeus schmitti; Litopenaeus vannamei.	
Categoria do assunto: 	X Pesquisa, Tecnologia e Engenharia	
Registro original: 	Embrapa Meio-Norte (CPAMN)	
Exemplares: 	CPAMN - (27843-1) - UPC - AP - PP - S 136/12 -  - 2012.00136 - DIS