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Registro Completo |
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
07/11/2019 |
Data da última atualização: |
07/11/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ANTUNES, G. dos R.; SANTANA, S. R. A.; ESCOBAR, I. E. C.; BRASIL, M. da S.; ARAUJO, G. G. L. de; VOLTOLINI, T. V.; FERNANDES JUNIOR, P. I. |
Afiliação: |
Gabiane dos Reis Antunes, UNIVASF; Sheilla Rios Assis Santana, UNIVASF; Indra Elena Costa Escobar, UNIVASF; Marivaine da Silva Brasil, Universidade Federal do Mato Grosso do Sul, Corumbá, MS; GHERMAN GARCIA LEAL DE ARAUJO, CPATSA; TADEU VINHAS VOLTOLINI, CPATSA; PAULO IVAN FERNANDES JUNIOR, CPATSA. |
Título: |
Associative diazotrophic bacteria from forage grasses in theBrazilian semi-arid region are effective plant growth promoters. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Crop & Pasture Science, v. 70, p. 899-907, 2019. |
DOI: |
10.1071/CP19076 |
Idioma: |
Inglês |
Conteúdo: |
The study of plant growth-promoting bacteria (PGPB) can identify outstanding bacteria for crops. For forage grasses adapted to drylands, the selection of PGPB can increase the field performance of pastures. The aim of this study was to isolate, and characterise at molecular, biochemical and symbiotic levels, diazotrophic bacteria obtained from buffel grass (Cenchrus ciliaris), sorghum (Sorghum bicolor) and Tifton 85 (Cynodon spp.) from Brazilian semi-arid region fields. Fieldgrown plants were collected, and the roots were surface-disinfected, crushed and inoculated in a semi-solid medium. After the formation and confirmation of microaerophilic pellicles, the bacteria were isolated and purified. All bacterial isolates were subjected to nifH gene amplification and identified by their partial 16S rRNA gene sequences. The bacteria were evaluated for the production of auxins and siderophores, calcium phosphate solubilisation, and diazotrophic ability as ?in vitro? plant growth-promotion traits. A plant inoculation assay was conducted to assess the plant growth promotion abilities of the bacterial isolates. Twenty-one bacterial isolates harboured the nifH gene (nifH+), among which nine were obtained from sorghum, eight from buffel grass, and four from Tifton 85. The bacterial isolates were classified as Bacillus (8), Stenotrophomonas (7), Agrobacterium (4), Cellulomonas (1) and Paenibacillus (1). All were shown to be auxin producers, with 14 isolates showing diazotrophic capacity ?in vitro?. Fourteen isolates increased plant N content, but the bacterial strains ESA 392 and ESA 398 (Bacillus), ESA 397 and ESA 407 (Stenotrophomonas), and ESA 401 (Agrobacterium) were shown to promote both plant growth and N nutrition. These strains are candidates for further assays to evaluate their agronomic performance under field conditions, aiming inoculant production for forage grasses in drylands. MenosThe study of plant growth-promoting bacteria (PGPB) can identify outstanding bacteria for crops. For forage grasses adapted to drylands, the selection of PGPB can increase the field performance of pastures. The aim of this study was to isolate, and characterise at molecular, biochemical and symbiotic levels, diazotrophic bacteria obtained from buffel grass (Cenchrus ciliaris), sorghum (Sorghum bicolor) and Tifton 85 (Cynodon spp.) from Brazilian semi-arid region fields. Fieldgrown plants were collected, and the roots were surface-disinfected, crushed and inoculated in a semi-solid medium. After the formation and confirmation of microaerophilic pellicles, the bacteria were isolated and purified. All bacterial isolates were subjected to nifH gene amplification and identified by their partial 16S rRNA gene sequences. The bacteria were evaluated for the production of auxins and siderophores, calcium phosphate solubilisation, and diazotrophic ability as ?in vitro? plant growth-promotion traits. A plant inoculation assay was conducted to assess the plant growth promotion abilities of the bacterial isolates. Twenty-one bacterial isolates harboured the nifH gene (nifH+), among which nine were obtained from sorghum, eight from buffel grass, and four from Tifton 85. The bacterial isolates were classified as Bacillus (8), Stenotrophomonas (7), Agrobacterium (4), Cellulomonas (1) and Paenibacillus (1). All were shown to be auxin producers, with 14 isolates showing diazotrophic capacity ... Mostrar Tudo |
Palavras-Chave: |
Bactérias diazotróficas; Crescimento vegetal; Fixação biológica de nitrogênio; PGPB; Seleção de linhagem; Semiárido. |
Thesagro: |
Bactéria; Caatinga; Capim Buffel; Forragem; Gramínea Forrageira; Sorgo. |
Thesaurus Nal: |
Forage grasses. |
Categoria do assunto: |
S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/204359/1/Associative-diazotrophic-bacteria-from-forage-2019.pdf
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Marc: |
LEADER 02992naa a2200361 a 4500 001 2113997 005 2019-11-07 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1071/CP19076$2DOI 100 1 $aANTUNES, G. dos R. 245 $aAssociative diazotrophic bacteria from forage grasses in theBrazilian semi-arid region are effective plant growth promoters.$h[electronic resource] 260 $c2019 520 $aThe study of plant growth-promoting bacteria (PGPB) can identify outstanding bacteria for crops. For forage grasses adapted to drylands, the selection of PGPB can increase the field performance of pastures. The aim of this study was to isolate, and characterise at molecular, biochemical and symbiotic levels, diazotrophic bacteria obtained from buffel grass (Cenchrus ciliaris), sorghum (Sorghum bicolor) and Tifton 85 (Cynodon spp.) from Brazilian semi-arid region fields. Fieldgrown plants were collected, and the roots were surface-disinfected, crushed and inoculated in a semi-solid medium. After the formation and confirmation of microaerophilic pellicles, the bacteria were isolated and purified. All bacterial isolates were subjected to nifH gene amplification and identified by their partial 16S rRNA gene sequences. The bacteria were evaluated for the production of auxins and siderophores, calcium phosphate solubilisation, and diazotrophic ability as ?in vitro? plant growth-promotion traits. A plant inoculation assay was conducted to assess the plant growth promotion abilities of the bacterial isolates. Twenty-one bacterial isolates harboured the nifH gene (nifH+), among which nine were obtained from sorghum, eight from buffel grass, and four from Tifton 85. The bacterial isolates were classified as Bacillus (8), Stenotrophomonas (7), Agrobacterium (4), Cellulomonas (1) and Paenibacillus (1). All were shown to be auxin producers, with 14 isolates showing diazotrophic capacity ?in vitro?. Fourteen isolates increased plant N content, but the bacterial strains ESA 392 and ESA 398 (Bacillus), ESA 397 and ESA 407 (Stenotrophomonas), and ESA 401 (Agrobacterium) were shown to promote both plant growth and N nutrition. These strains are candidates for further assays to evaluate their agronomic performance under field conditions, aiming inoculant production for forage grasses in drylands. 650 $aForage grasses 650 $aBactéria 650 $aCaatinga 650 $aCapim Buffel 650 $aForragem 650 $aGramínea Forrageira 650 $aSorgo 653 $aBactérias diazotróficas 653 $aCrescimento vegetal 653 $aFixação biológica de nitrogênio 653 $aPGPB 653 $aSeleção de linhagem 653 $aSemiárido 700 1 $aSANTANA, S. R. A. 700 1 $aESCOBAR, I. E. C. 700 1 $aBRASIL, M. da S. 700 1 $aARAUJO, G. G. L. de 700 1 $aVOLTOLINI, T. V. 700 1 $aFERNANDES JUNIOR, P. I. 773 $tCrop & Pasture Science$gv. 70, p. 899-907, 2019.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registros recuperados : 7 | |
1. | | VOLTOLINI, T. V.; SANTANA, S. R. A.; ANTUNUES, G. dos R.; ARAUJO, G. G. L. de. Alternativas alimentares para os rebanhos. In: MELO, R. F. de; VOLTOLINI, T. V. (Ed.). Agricultura familiar dependente de chuva no Semiárido. Brasília, DF, Embrapa, 2019. cap. 7, p. 229-261.Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Semiárido. |
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2. | | ANTUNES, G. dos R.; SANTANA, S. R. A.; ESCOBAR, I. E. C.; BRASIL, M. da S.; ARAUJO, G. G. L. de; VOLTOLINI, T. V.; FERNANDES JUNIOR, P. I. Associative diazotrophic bacteria from forage grasses in theBrazilian semi-arid region are effective plant growth promoters. Crop & Pasture Science, v. 70, p. 899-907, 2019.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Semiárido. |
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3. | | ANTUNES, G. dos R.; VOLTOLINI, T. V.; ARAUJO, G. G. L. de; ESCOBAR, I. E. C.; CAVALCANTI, M. I. P.; OLIVEIRA, K. G.; NASCIMENTO, T. R. do; SANTANA, S. R. A.; FERNANDES JUNIOR, P. I. Caracterização fenotípica de bactérias Diazotróficas Endofíticas isoladas de capim-buffel no Semiárido de Pernambuco. In: SIMPÓSIO DE MUDANÇAS CLIMÁTICAS E DESERTIFICAÇÃO NO SEMIÁRIDO BRASILEIRO, 4., 2015, Petrolina. Experiências e oportunidades para o desenvolvimento. Petrolina: Embrapa Semiárido, 2015. 1 CD-ROM. (Embrapa Semiárido. Documentos, 262).Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Semiárido. |
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4. | | SANTANA, S. R. A.; VOLTOLINI, T. V.; ANTUNES, G. dos R.; SILVA, V. M. da; SIMOES, W. L.; MORGANTE, C. V.; FREITAS, A. D. S. de; CHAVES, A. R. de M.; AIDAR, S. de T.; FERNANDES JUNIOR, P. I. Inoculation of plant growth promoting bacteria attenuates the negative efects of drought on sorghum. Archives of Microbiology, v. 202, p. 1015-1024, 2020.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Semiárido. |
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5. | | CAMPOS, L. J. M.; COSTA, R. V.; ALMEIDA, R. E. M.; EVANGELISTA, B. A.; EVARISTO, A. B.; SANTOS, D.; SILVA, V. O. O.; TUBIANA, D. O.; SANTANA, S. R.; REIS, I. A. Influência do espaçamento na produtividade da soja em várzeas tropicais. In: REUNIÃO DE PESQUISA DE SOJA, 37., 2019, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2019. p. 18-21. (Embrapa Soja. Documentos, 413).Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Pesca e Aquicultura. |
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6. | | CAMPOS, L. J. M.; COSTA, R. V.; ALMEIDA, R. E. M.; EVANGELISTA, B. A.; EVARISTO, A. B.; SANTOS, D.; SILVA, V. O. O.; TUBIANA, D. O.; SANTANA, S. R.; REIS, I. A. Influência do espaçamento na produtividade da soja em várzeas tropicais. In: REUNIÃO DE PESQUISA DE SOJA, 37., 2019, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2019. p. 18-21. (Embrapa Soja. Documentos, 413).Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Milho e Sorgo. |
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7. | | CAMPOS, L. J. M.; COSTA, R. V.; ALMEIDA, R. E. M.; EVANGELISTA, B. A.; EVARISTO, A. B.; SANTOS, D.; SILVA, V. O. O.; TUBIANA, D. O.; SANTANA, S. R.; REIS, I. A. Influência do espaçamento na produtividade da soja em várzeas tropicais. In: REUNIÃO DE PESQUISA DE SOJA, 37., 2019, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2019. (Embrapa Soja. Documentos, 413). p. 18-21.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
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Registros recuperados : 7 | |
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