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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
20/04/2004 |
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Data da última atualização: |
20/04/2004 |
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Autoria: |
SANTANA, M. S.; MATHEUS, J. |
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Título: |
In situ inmunofluorescent localization of linamarase in cassava (Manihot esculenta Crantz). |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Revista Brasileira de Mandioca, Salvador, v.17, p.76, nov., 1998. Suplemento. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Cassava (Manihot esculenta Crantz) is a cianogenic species consumed as a major staple food in many tropical countries. Cianogenic glucosides are synthesized in leaf tissues tranported to starch roots where they are stored. Mechanical demage of cassava tissues leads to the release of hydrocyanic acid (HCN), a volatile highly toxic-compound. Under normal physiological conditions cassava tissues contains no detectable HCN since the cianogenic glucosides and its degrading enzymes, linamarase and hydroxynitrile lyase, exists in different in compartments. Compartmentation of linamarase has been studied previously by other authors in several species measuring enzyme activity in different tissues. In leaves, linamarase is reported to be entirely apoplastic and associated to the cell walls. However, Pancoro and Hunghes (1992) detected the expression of mRNA and the activity of linamarase only in laticifers of cassava leaves. The present study investigates the location of linamarase in leaves and roots of two varieties of cassava of low and high content of cianoglucosides by inmunoflurescent labelling. Anti-linamarase immure e serum was obtained by inoculating a rabbit with purified linamarase. Hand-cults tissues were incubated with the anti-linamarase serum and then incubated with a commercial FITC-anti-rabbit IgG conjugate. Pre-inmune serum was control. The results shows the inmunoflurescent label distributed mainly in laticifers of leaves and roots in both varieties with no differences between then. Our results supports those of Pancoro and Hughes (1992) and in addition suggest that more than one tissue mary be involved in the synthesis and transport of linamarase. MenosAbstract: Cassava (Manihot esculenta Crantz) is a cianogenic species consumed as a major staple food in many tropical countries. Cianogenic glucosides are synthesized in leaf tissues tranported to starch roots where they are stored. Mechanical demage of cassava tissues leads to the release of hydrocyanic acid (HCN), a volatile highly toxic-compound. Under normal physiological conditions cassava tissues contains no detectable HCN since the cianogenic glucosides and its degrading enzymes, linamarase and hydroxynitrile lyase, exists in different in compartments. Compartmentation of linamarase has been studied previously by other authors in several species measuring enzyme activity in different tissues. In leaves, linamarase is reported to be entirely apoplastic and associated to the cell walls. However, Pancoro and Hunghes (1992) detected the expression of mRNA and the activity of linamarase only in laticifers of cassava leaves. The present study investigates the location of linamarase in leaves and roots of two varieties of cassava of low and high content of cianoglucosides by inmunoflurescent labelling. Anti-linamarase immure e serum was obtained by inoculating a rabbit with purified linamarase. Hand-cults tissues were incubated with the anti-linamarase serum and then incubated with a commercial FITC-anti-rabbit IgG conjugate. Pre-inmune serum was control. The results shows the inmunoflurescent label distributed mainly in laticifers of leaves and roots in both varieties with ... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 02117naa a2200133 a 4500
001 1651949
005 2004-04-20
008 1998 bl uuuu u00u1 u #d
100 1 $aSANTANA, M. S.
245 $aIn situ inmunofluorescent localization of linamarase in cassava (Manihot esculenta Crantz).
260 $c1998
520 $aAbstract: Cassava (Manihot esculenta Crantz) is a cianogenic species consumed as a major staple food in many tropical countries. Cianogenic glucosides are synthesized in leaf tissues tranported to starch roots where they are stored. Mechanical demage of cassava tissues leads to the release of hydrocyanic acid (HCN), a volatile highly toxic-compound. Under normal physiological conditions cassava tissues contains no detectable HCN since the cianogenic glucosides and its degrading enzymes, linamarase and hydroxynitrile lyase, exists in different in compartments. Compartmentation of linamarase has been studied previously by other authors in several species measuring enzyme activity in different tissues. In leaves, linamarase is reported to be entirely apoplastic and associated to the cell walls. However, Pancoro and Hunghes (1992) detected the expression of mRNA and the activity of linamarase only in laticifers of cassava leaves. The present study investigates the location of linamarase in leaves and roots of two varieties of cassava of low and high content of cianoglucosides by inmunoflurescent labelling. Anti-linamarase immure e serum was obtained by inoculating a rabbit with purified linamarase. Hand-cults tissues were incubated with the anti-linamarase serum and then incubated with a commercial FITC-anti-rabbit IgG conjugate. Pre-inmune serum was control. The results shows the inmunoflurescent label distributed mainly in laticifers of leaves and roots in both varieties with no differences between then. Our results supports those of Pancoro and Hughes (1992) and in addition suggest that more than one tissue mary be involved in the synthesis and transport of linamarase.
700 1 $aMATHEUS, J.
773 $tRevista Brasileira de Mandioca, Salvador$gv.17, p.76, nov., 1998. Suplemento.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 9 | |
| 3. |  | MENDES, I. de C.; VIVALDI, L.; SANTANA, M. S. Propriedades microbiologicas de solos do bioma cerrado sob vegetacao nativa. In: REUNIAO BRAS. FERTILIDADE DO SOLO E NUTRICAO DE PLANTAS, 25.; REUNIAO BRASILEIRA SOBRE MICORRIZAS,8.; SIMPOSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 6.; REUNIAO BRASILEIRA DE BIOLOGIA DO SOLO, 3., 2000, Santa Maria, RS. Fertbio 2000: biodinamica do solo. [S.l.: s.n.], 2000. nao paginado.| Biblioteca(s): Embrapa Cerrados. |
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| 5. | | SANTANA, M. S.; GOMES, J. S.; ROSINHA, G. M. S.; SOARES, C. O.; COELHO, M. B. Imunização com vacina de DNA contra Linfadenite Caseosa e análise da resposta imune em camundongos. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 13., 2017, Campo Grande, MS. [Anais...] Brasília, DF: Embrapa, 2017. p. 70. (Embrapa Gado de Corte, Documentos, 242)| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 6. | | SANTANA, M. S.; GIONGO, V.; BRANDÃO, S. S.; JESUS, T. S.; MENEZES, R. S. C.; CUNHA, T. J. F. Carbon stock change due to land use in argisols on pernambucano-brazillan semiarid In: CONGRESO LATINOAMERICANO DE LA CIENCIA DEL SUELO, 20.; CONGRESO PERUANO DE LA CIENCIA DEL SUELO, 16., 2014, Cusco. Educar para preservar el suelo y conservar la vida en la tierra. Cusco: Centro de Convenciones de la Municipalidad del Cusco, 2014. 1 Pen drive.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 7. | | SANTOS, L. G. dos; BALDANI, V. L. D.; FERREIRA, J. S.; BAHIA, B. L.; SANTANA, M. S.; PEIXOUTO, L. S. Sobrevivência de bactérias diazotróficas em suporte inoculante alternativo de casca de algodão. Conjecturas, v. 22, n. 2, p. 1389-1397, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 8. | | CAITANO, M. A. B.; BASTOS, R.; COELHO, M. B.; DIEHL, L. de O.; SANTOS, L. R. dos; SANTANA, M. S.; ROSINHA, G. M. S. Avaliação do sistema de entrega e estabilidade de uma vacina de DNA contendo o gene ssiv DE Brucella abortus, encapsulada em nanopartículas de quitosana. In: SIMPÓSIO DE IMUNOLOGIA DO CENTRO-OESTE, I., Goiânia, 2017. Revista de Patologia Tropical, v. 46, supl. 1, p. 16, 2017.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 9. | | ANDRADE, L. R. M. de; MARTINS, E. de S.; MENDES, I. de C.; RODRIGUES, L. O.; ARAUJO, R.; SANTANA, M. S. Poltencial da rocha carbonatituo como fertilizabte e corretivo de acidez para solos dos cerrados. In: CONGRESSO BRASILEIRO DE CIENCIA DO SOLO, 28., 2001, Londrina, PR. Ciencia do solo: fator de produtividade competitiva com sustentabilidade. Londrina: SBCS, 2001. p. 167.| Biblioteca(s): Embrapa Cerrados. |
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| Registros recuperados : 9 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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