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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
24/02/2015 |
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Data da última atualização: |
23/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BRAGA, R. M.; SANTANA, M. F.; COSTA, R. V. da; BROMMONSCHENKEL, S. H.; ARAÚJO, E. F. de; QUEIROZ, M. V. de. |
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Afiliação: |
RODRIGO VERAS DA COSTA, CNPMS. |
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Título: |
Transposable elements belonging to the Tc1-Mariner superfamily are heavily mutated in Colletotrichum graminicola. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Mycologia, New York, v. 106, n. 4, p. 629-641, 2014. |
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DOI: |
10.3852/13?262 |
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Idioma: |
Inglês |
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Conteúdo: |
Transposable elements are ubiquitous and constitute an important source of genetic variation in addition to generating deleterious mutations. Several filamentous fungi are able to defend against transposable elements using RIP(repeat-induced point mutation)-like mechanisms, which induce mutations in duplicated sequences. The sequenced Colletotrichum graminicola genome and the availability of transposable element databases provide an efficient approach for identifying and characterizing transposable elements in this fungus, which was the subject of this study. We identified 132 full-sized Tc1-Mariner transposable elements in the sequenced C. graminicola genome, which were divided into six families. Several putative transposases that have been found in these elements have conserved DDE motifs, but all are interrupted by stop codons. An in silico analysis showed evidence for RIP-generated mutations. The TCg1 element, which was cloned from the Brazilian 2908 m isolate, has a putative transposase sequence with three characteristic conserved motifs. However, this sequence is interrupted by five stop codons. Genomic DNA from various isolates was analyzed by hybridization with an internal region of TCg1. All of the isolates featured transposable elements that were similar to TCg1, and several hybridization profiles were identified. C. graminicola has many Tc1-Mariner transposable elements that have been degenerated by characteristic RIP mutations. It is unlikely that any of the characterized elements are autonomous in the sequenced isolate. The possible existence of active copies in field isolates from Brazil was shown. The TCg1 element is present in severalC. graminicola isolates and is a potentially useful molecular marker for population studies of this phytopathogen. Key words: RIP, transposase, transposon MenosTransposable elements are ubiquitous and constitute an important source of genetic variation in addition to generating deleterious mutations. Several filamentous fungi are able to defend against transposable elements using RIP(repeat-induced point mutation)-like mechanisms, which induce mutations in duplicated sequences. The sequenced Colletotrichum graminicola genome and the availability of transposable element databases provide an efficient approach for identifying and characterizing transposable elements in this fungus, which was the subject of this study. We identified 132 full-sized Tc1-Mariner transposable elements in the sequenced C. graminicola genome, which were divided into six families. Several putative transposases that have been found in these elements have conserved DDE motifs, but all are interrupted by stop codons. An in silico analysis showed evidence for RIP-generated mutations. The TCg1 element, which was cloned from the Brazilian 2908 m isolate, has a putative transposase sequence with three characteristic conserved motifs. However, this sequence is interrupted by five stop codons. Genomic DNA from various isolates was analyzed by hybridization with an internal region of TCg1. All of the isolates featured transposable elements that were similar to TCg1, and several hybridization profiles were identified. C. graminicola has many Tc1-Mariner transposable elements that have been degenerated by characteristic RIP mutations. It is unlikely that any of the char... Mostrar Tudo |
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Thesagro: |
Antracnose. |
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Categoria do assunto: |
-- |
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Marc: |
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100 1 $aBRAGA, R. M.
245 $aTransposable elements belonging to the Tc1-Mariner superfamily are heavily mutated in Colletotrichum graminicola.$h[electronic resource]
260 $c2014
520 $aTransposable elements are ubiquitous and constitute an important source of genetic variation in addition to generating deleterious mutations. Several filamentous fungi are able to defend against transposable elements using RIP(repeat-induced point mutation)-like mechanisms, which induce mutations in duplicated sequences. The sequenced Colletotrichum graminicola genome and the availability of transposable element databases provide an efficient approach for identifying and characterizing transposable elements in this fungus, which was the subject of this study. We identified 132 full-sized Tc1-Mariner transposable elements in the sequenced C. graminicola genome, which were divided into six families. Several putative transposases that have been found in these elements have conserved DDE motifs, but all are interrupted by stop codons. An in silico analysis showed evidence for RIP-generated mutations. The TCg1 element, which was cloned from the Brazilian 2908 m isolate, has a putative transposase sequence with three characteristic conserved motifs. However, this sequence is interrupted by five stop codons. Genomic DNA from various isolates was analyzed by hybridization with an internal region of TCg1. All of the isolates featured transposable elements that were similar to TCg1, and several hybridization profiles were identified. C. graminicola has many Tc1-Mariner transposable elements that have been degenerated by characteristic RIP mutations. It is unlikely that any of the characterized elements are autonomous in the sequenced isolate. The possible existence of active copies in field isolates from Brazil was shown. The TCg1 element is present in severalC. graminicola isolates and is a potentially useful molecular marker for population studies of this phytopathogen. Key words: RIP, transposase, transposon
650 $aAntracnose
700 1 $aSANTANA, M. F.
700 1 $aCOSTA, R. V. da
700 1 $aBROMMONSCHENKEL, S. H.
700 1 $aARAÚJO, E. F. de
700 1 $aQUEIROZ, M. V. de
773 $tMycologia, New York$gv. 106, n. 4, p. 629-641, 2014.
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Embrapa Milho e Sorgo (CNPMS) |
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| 7. |  | SANTANA, M. F.; ZAMBOLIM, E. M.; OLIVEIRA, L. O. de; CAIXETA, E. T.; ZAMBOLIM, L. Análise molecular do rDNA de Hemileia vastatrix. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 5., 2007, Águas de Lindóia, SP. Anais... Brasília, DF: Embrapa Café, 2007.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 10. | | BREDA, M. O.; OLIVEIRA, J. V. de; MARQUES, E. J.; FERREIRA, R. G.; SANTANA, M. F. Inseticidas botânicos aplicados sobre Aphis gossypii e seu predador Cycloneda sanguinea em algodão-colorido. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 46, n. 11, p. 1424-1431, out. 2011 Título em inglês: Botanical insecticides applied on Aphis gossypii and its predator Cycloneda sanguinea on naturally colored cotton.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 14. | | MAIA, T. A.; ZAMBOLIM, E. M.; CAIXETA, E. T.; MIZUBUTI, E. S. G.; SANTANA, M. F.; ZAMBOLIM, L. Estrutura genética da população de Hemileia vastatrix com base no marcador AFLP. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 6., 2009, Vitória. Anais...Brasília, DF: Embrapa Café, 2009.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 15. | | MEYER, G.; CORREA, R. de O.; MARTINS JUNIOR, H.; SILVA, R. M.; COSTA, S. M.; SANTANA, M. F. S. Tambacus (Colossoma macropomum x Piaractus mesopotamicus) alimentados com rações artesanais de macaxeira e soja. Archivos de Zootecnia, v. 68, n. 263, p. 376-382, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 16. | | SANTANA, M. F.; SILVA, J. C. F.; BATISTA, A. D.; RIBEIRO, L. E.; SILVA, G. F. da; ARAÚJO, E. F. de; QUEIROZ, M. V. de. Abundance, distribution and potential impact of transposable elements in the genome of Mycosphaerella fijiensis. BMC Genomics, v. 13, n. 1, p. 1-11, Dec. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 17. | | NEVES, G. A. R.; SANTANA, M. F. S.; CARVALHO, J. E. U.; NASCIMENTO, W. M. O. do; VALENÇA, R. S. V. Caracterização físico-química de frutos de murici na Região Norte do Brasil. In: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS, 8., 2009, Campinas. Ciência de alimentos no mundo globalizado: novos desafios, novas perspectivas. Campinas: Unicamp, 2009. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 18. | | OLIVEIRA, L. C.; VASCONCELOS, M. A. M.; RODRIGUES, M. C. S. F; SANTANA, M. F. S.; PEROTES, K. F.; PERICLES, A.; MATOS, G. B. Avaliação preliminar das caracteristicas físicas e químicas de frutos e polpa de golosa (Chrysophyllum cuneifolium (Rudge) A. DC.) In: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS, 8., 2009, Campinas. Ciência de alimentos no mundo globalizado: novos desafios, novas perspectivas. Campinas: Unicamp, 2009. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 19. | | SILVA, G. F. da; PAIXÃO, R. D. V.; QUEIROZ, C. B.; SANTANA, M. F.; SOUZA, A.; SOUSA, N. R.; HANADA, R. E.; GASPAROTTO, L. Genetic diversity of Mycosphaerella fijiensis in Brazil analyzed using an ERIC-PCR marker. Genetics and Molecular Research, v. 13, n. 3, p. 7698-7707, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 20. | | ABADIO, A. K. R.; LIMA, S. S.; SANTANA, M. F.; SALOMÃO, T. M. F.; SARTORATO, A.; MIZUBUTI, E. S. G.; ARAÚJO, E. F.; QUEIROZ, M. V. de. Genetic diversity analysis of isolates of the fungal bean pathogen Pseudocercospora griseola from central and southern Brazil. Genetics and Molecular Research, v. 11, n. 2, p. 1272-1279, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 25 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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