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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
30/10/2015 |
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Data da última atualização: |
30/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VIEIRA, L. de J.; SANTANA, J. R. F. de; ALVES, A. A. C.; LEDO, C. A. da S.; SOUZA, F. V. D. |
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Afiliação: |
LÍVIA DE JESUS VIEIRA, UEFS; JOSÉ RANIERE FERREIRA DE SANTANA, UEFS; ALFREDO AUGUSTO CUNHA ALVES, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
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Título: |
Use of aniline blue stain to observing pollen tubes development in different Manihot Mill. species. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
African Journal of Agricultural Research, v. 10, n. 15, p. 1805-1809, 9 April, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
The lack of a reliable test to evaluate pollen viability in species of the genus Manihot is one of the primary limitations for cassava genetic improvement. Thus, the objective of the present study was to apply aniline blue stain to observing pollen tubes development in different Manihot Mill. species, in order to estimate the crossability, pollen germination time, pollen tube size and pollen longevity. Pollen grains from 2 accessions of the cultivated species M. esculenta spp. esculenta and 4 accessions of 3 wild species (M. esculenta ssp. flabellifolia (Pohl) Cif, M. anomala Pohl, and M. irwinii D. J. Roger and Appan) were used. To visualize the pollen tubes, pollinated stigmas was deposited on a glass slide with 3 drops of aniline blue stain and transferred to an optical fluorescence microscope, where they were visualized and photographed using the 10x ocular lens. The pollen crossability rate was estimated by the ercentage of germinated pollen, with well-developed pollen tubes in relation to the total number of pollen grains counted per stigma. The experimental design was entirely random with 3 repetitions, each repetition consisting of 100 pollen grains. In addition to the estimation of crossability, the germination time after pollination, pollen tube size, and pollen longevity were also determined to the accession FLA 029V-01. Significant difference was observed between the crossings. Pollen tubes developed after 45 min of contact between the pollen and the stigma. The average pollen tube size 24 h after pollination was 2.9 mm. The studies examining pollen longevity showed that germination did not occur when the pollen grains were evaluated 24 h after anthesis. Thus, the use of aniline blue stain can be considered an effective method for observing pollen tubes development and studying the pollen morphology, longevity, viability and Manihot crossability. MenosThe lack of a reliable test to evaluate pollen viability in species of the genus Manihot is one of the primary limitations for cassava genetic improvement. Thus, the objective of the present study was to apply aniline blue stain to observing pollen tubes development in different Manihot Mill. species, in order to estimate the crossability, pollen germination time, pollen tube size and pollen longevity. Pollen grains from 2 accessions of the cultivated species M. esculenta spp. esculenta and 4 accessions of 3 wild species (M. esculenta ssp. flabellifolia (Pohl) Cif, M. anomala Pohl, and M. irwinii D. J. Roger and Appan) were used. To visualize the pollen tubes, pollinated stigmas was deposited on a glass slide with 3 drops of aniline blue stain and transferred to an optical fluorescence microscope, where they were visualized and photographed using the 10x ocular lens. The pollen crossability rate was estimated by the ercentage of germinated pollen, with well-developed pollen tubes in relation to the total number of pollen grains counted per stigma. The experimental design was entirely random with 3 repetitions, each repetition consisting of 100 pollen grains. In addition to the estimation of crossability, the germination time after pollination, pollen tube size, and pollen longevity were also determined to the accession FLA 029V-01. Significant difference was observed between the crossings. Pollen tubes developed after 45 min of contact between the pollen and the stigma. The ... Mostrar Tudo |
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Thesagro: |
Mandioca; Melhoramento vegetal; Recurso genético. |
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Thesaurus Nal: |
Breeding methods; Cassava; Genetic resources. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02645naa a2200241 a 4500
001 2027633
005 2023-05-30
008 2015 bl uuuu u00u1 u #d
100 1 $aVIEIRA, L. de J.
245 $aUse of aniline blue stain to observing pollen tubes development in different Manihot Mill. species.$h[electronic resource]
260 $c2015
520 $aThe lack of a reliable test to evaluate pollen viability in species of the genus Manihot is one of the primary limitations for cassava genetic improvement. Thus, the objective of the present study was to apply aniline blue stain to observing pollen tubes development in different Manihot Mill. species, in order to estimate the crossability, pollen germination time, pollen tube size and pollen longevity. Pollen grains from 2 accessions of the cultivated species M. esculenta spp. esculenta and 4 accessions of 3 wild species (M. esculenta ssp. flabellifolia (Pohl) Cif, M. anomala Pohl, and M. irwinii D. J. Roger and Appan) were used. To visualize the pollen tubes, pollinated stigmas was deposited on a glass slide with 3 drops of aniline blue stain and transferred to an optical fluorescence microscope, where they were visualized and photographed using the 10x ocular lens. The pollen crossability rate was estimated by the ercentage of germinated pollen, with well-developed pollen tubes in relation to the total number of pollen grains counted per stigma. The experimental design was entirely random with 3 repetitions, each repetition consisting of 100 pollen grains. In addition to the estimation of crossability, the germination time after pollination, pollen tube size, and pollen longevity were also determined to the accession FLA 029V-01. Significant difference was observed between the crossings. Pollen tubes developed after 45 min of contact between the pollen and the stigma. The average pollen tube size 24 h after pollination was 2.9 mm. The studies examining pollen longevity showed that germination did not occur when the pollen grains were evaluated 24 h after anthesis. Thus, the use of aniline blue stain can be considered an effective method for observing pollen tubes development and studying the pollen morphology, longevity, viability and Manihot crossability.
650 $aBreeding methods
650 $aCassava
650 $aGenetic resources
650 $aMandioca
650 $aMelhoramento vegetal
650 $aRecurso genético
700 1 $aSANTANA, J. R. F. de
700 1 $aALVES, A. A. C.
700 1 $aLEDO, C. A. da S.
700 1 $aSOUZA, F. V. D.
773 $tAfrican Journal of Agricultural Research$gv. 10, n. 15, p. 1805-1809, 9 April, 2015.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| 9. |  | RIBEIRO, S. M.; ROSINHA, G. M. S.; SANTOS, L. R. dos; SANTOS, G. M. Avaliação da resposta imune de camundongos BALB/c a uma proteína recombinante visando o desenvolvimento de vacina contra tristeza parasitária bovina. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 11., 2015, Campo Grande, MS. Anais... Campo Grande: Embrapa Gado de Corte, 2015. 114 p. (Embrapa Gado de Corte. Documentos, 213).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 10. | | ROSINHA, G. M. S.; ELISEI, C.; GALVÃO, C.; SOARES, C. O.; ARAUJO, F. R. Avaliação de polimorfismos de DNA no gene prnp e sua associação à EEB e scrapie em bovinos e ovinos de raças brasileiras, respectivamente. In: SIMPÓSIO EMBRAPA LABEX DE SANIDADE ANIMAL, 1., 2009, Campo Grande, MS. Anais... Campo Grande, MS: Embrapa Gado de Corte, 2009. 2 p. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 11. | | UMEDA, L. M. L.; SOARES, C. O.; BASTOS, R.; JÁCOMO, C. S.; ROSINHA, G. M. S. Avaliação por PCR da persistência da vacina S19 em bezerras vacinadas. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 8., 2012, Campo Grande, MS. [Anais da..]. Campo Grande, MS: Embrapa Gado de Corte, 2012. 2 p. (Embrapa Gado de Corte. Documentos, 198).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 12. | | ROSINHA, G. M. S.; ELISEI, C.; BASTOS, R.; FORNER, O.; SOARES, C. O. Análise da variabilidade intraespécie dos genes PLD e RPOB de Corynebacterium pseudotuberculosis por meio de marcadores PCR-RFLP. In: XIMENES, L. J. F.; MARTINS, G. A.; MORAIS, O. R. de; COSTA, . S. de A.; NASCIMENTO, J. L. S. do (Coord.). Ciência e tecnologia na pecuária de caprinos ovinos. Fortaleza: Banco do Nordeste do Brasil, 2010. p. 581-591 (Série BNB. Ciência e Tecnologia, 5).| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 17. | | SANTANA, M. S.; GOMES, J. S.; ROSINHA, G. M. S.; SOARES, C. O.; COELHO, M. B. Imunização com vacina de DNA contra Linfadenite Caseosa e análise da resposta imune em camundongos. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 13., 2017, Campo Grande, MS. [Anais...] Brasília, DF: Embrapa, 2017. p. 70. (Embrapa Gado de Corte, Documentos, 242)| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 18. | | BASTOS, R. B. P.; SOARES, C. O.; SANTOS, L. R. dos; ROSINHA, G. M. S. Desenvolvimento e avaliação de vacinas de DNA contra Brucella abortus. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 8., 2012, Campo Grande, MS. [Anais da..]. Campo Grande, MS: Embrapa Gado de Corte, 2012. 2 p. (Embrapa Gado de Corte. Documentos, 198).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 19. | | SILVA, P. M. P.; SILVA, M. R.; SANTOS, M. G. dos; ROSINHA, G. M. S. Detecção de Brucella spp. em queijos artesanais pela técnica da reação em cadeia da polimerase. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 11., 2015, Campo Grande, MS. Anais... Campo Grande: Embrapa Gado de Corte, 2015. 114 p. (Embrapa Gado de Corte. Documentos, 213).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 20. | | SANCHES, C. C.; ROSINHA, G. M. S.; GALVÃO, C. E.; ELISEI, C.; SOARES, C. O. Mutante apolar 2308?virB10 de Brucella abortus induz proteção inferior em camundongons BALB/C, comparada às cepas vacinais B19 E RB51. In: JORNADA CIENTÍFICA DA EMBRAPA GADO DE CORTE, 6., 2010, Campo Grande, MS. Ética na pesquisa científica: [Anais da ...]. Campo Grande, MS: Embrapa Gado de Corte, 2010. 1 CD-ROM. Coordenadora: Vanessa Felipe de Souza 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| Registros recuperados : 174 | |
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