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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
17/12/2018 |
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Data da última atualização: |
18/02/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, I. H. S. da; GOMÉZ, I.; SÁNCHEZ, J.; CASTRO, D. L. M. de; VALICENTE, F. H.; SOBERÓN, M.; POLANCZYK, R. A.; BRAVO, A. |
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Afiliação: |
Igor Henrique Sena da Silva, Universidade Estadual Paulista; Isabel Goméz, Universidad Nacional Autónoma de México; Jorge Sánchez, Universidad Nacional Autónoma de México; Diana L. Martínez de Castro, Universidad Nacional Autónoma de México; FERNANDO HERCOS VALICENTE, CNPMS; Mario Soberón, Universidad Nacional Autónoma de México; Ricardo Antonio Polanczyk, Universidade Estadual Paulista; Alejandra Bravo, Universidad Nacional Autónoma de México. |
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Título: |
Identification of midgut membrane proteins from different instars of Helicoverpa armigera (Lepidoptera: Noctuidae) that bind to Cry1Ac toxin. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Plos One, San Francisco, v. 13, n. 12, e0207789, 2018. |
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DOI: |
10.1371/journal.pone.0207789 |
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Idioma: |
Inglês |
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Conteúdo: |
Helicoverpa armigera is a polyphagous pest sensitive to Cry1Ac protein from Bacillus thuringiensis (Bt). The susceptibility of the different larval instars of H. armigera to Cry1Ac protoxin showed a significant 45-fold reduction in late instars compared to early instars. A possible hypothesis is that gut surface proteins that bind to Cry1Ac differ in both instars, although higher Cry toxin degradation in late instars could also explain the observed differences in susceptibility. Here we compared the Cry1Ac-binding proteins from second and fifth instars by pull-down assays and liquid chromatography coupled to mass spectrometry analysis (LC-MS/MS). The data show differential protein interaction patterns of Cry1Ac in the two instars analyzed. Alkaline phosphatase, and other membrane proteins, such as prohibitin and an anion selective channel protein were identified only in the second instar, suggesting that these proteins may be involved in the higher toxicity of Cry1Ac in early instars of H. armigera. Eleven Cry1Ac binindg proteins were identified exclusively in late instar larvae, like different proteases such as trypsin-like protease, azurocidin-like proteinase, and carboxypeptidase. Different aminopeptidase N isofroms were identified in both instar larvae. We compared the Cry1Ac protoxin degradation using midgut juice from late and early instars, showing that the midgut juice from late instars is more efficient to degrade Cry1Ac protoxin than that of early instars, suggesting that increased proteolytic activity on the toxin could also explain the low Cry1Ac toxicity in late instars. MenosHelicoverpa armigera is a polyphagous pest sensitive to Cry1Ac protein from Bacillus thuringiensis (Bt). The susceptibility of the different larval instars of H. armigera to Cry1Ac protoxin showed a significant 45-fold reduction in late instars compared to early instars. A possible hypothesis is that gut surface proteins that bind to Cry1Ac differ in both instars, although higher Cry toxin degradation in late instars could also explain the observed differences in susceptibility. Here we compared the Cry1Ac-binding proteins from second and fifth instars by pull-down assays and liquid chromatography coupled to mass spectrometry analysis (LC-MS/MS). The data show differential protein interaction patterns of Cry1Ac in the two instars analyzed. Alkaline phosphatase, and other membrane proteins, such as prohibitin and an anion selective channel protein were identified only in the second instar, suggesting that these proteins may be involved in the higher toxicity of Cry1Ac in early instars of H. armigera. Eleven Cry1Ac binindg proteins were identified exclusively in late instar larvae, like different proteases such as trypsin-like protease, azurocidin-like proteinase, and carboxypeptidase. Different aminopeptidase N isofroms were identified in both instar larvae. We compared the Cry1Ac protoxin degradation using midgut juice from late and early instars, showing that the midgut juice from late instars is more efficient to degrade Cry1Ac protoxin than that of early instars, suggesti... Mostrar Tudo |
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Thesagro: |
Praga de Planta; Proteína; Toxina. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/188667/1/Identification-midgut.pdf
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Marc: |
LEADER 02401naa a2200253 a 4500
001 2101792
005 2019-02-18
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0207789$2DOI
100 1 $aSILVA, I. H. S. da
245 $aIdentification of midgut membrane proteins from different instars of Helicoverpa armigera (Lepidoptera$bNoctuidae) that bind to Cry1Ac toxin.$h[electronic resource]
260 $c2018
520 $aHelicoverpa armigera is a polyphagous pest sensitive to Cry1Ac protein from Bacillus thuringiensis (Bt). The susceptibility of the different larval instars of H. armigera to Cry1Ac protoxin showed a significant 45-fold reduction in late instars compared to early instars. A possible hypothesis is that gut surface proteins that bind to Cry1Ac differ in both instars, although higher Cry toxin degradation in late instars could also explain the observed differences in susceptibility. Here we compared the Cry1Ac-binding proteins from second and fifth instars by pull-down assays and liquid chromatography coupled to mass spectrometry analysis (LC-MS/MS). The data show differential protein interaction patterns of Cry1Ac in the two instars analyzed. Alkaline phosphatase, and other membrane proteins, such as prohibitin and an anion selective channel protein were identified only in the second instar, suggesting that these proteins may be involved in the higher toxicity of Cry1Ac in early instars of H. armigera. Eleven Cry1Ac binindg proteins were identified exclusively in late instar larvae, like different proteases such as trypsin-like protease, azurocidin-like proteinase, and carboxypeptidase. Different aminopeptidase N isofroms were identified in both instar larvae. We compared the Cry1Ac protoxin degradation using midgut juice from late and early instars, showing that the midgut juice from late instars is more efficient to degrade Cry1Ac protoxin than that of early instars, suggesting that increased proteolytic activity on the toxin could also explain the low Cry1Ac toxicity in late instars.
650 $aPraga de Planta
650 $aProteína
650 $aToxina
700 1 $aGOMÉZ, I.
700 1 $aSÁNCHEZ, J.
700 1 $aCASTRO, D. L. M. de
700 1 $aVALICENTE, F. H.
700 1 $aSOBERÓN, M.
700 1 $aPOLANCZYK, R. A.
700 1 $aBRAVO, A.
773 $tPlos One, San Francisco$gv. 13, n. 12, e0207789, 2018.
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Embrapa Milho e Sorgo (CNPMS) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
10/01/2020 |
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Data da última atualização: |
29/01/2020 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
DIAS, J. A.; PACHECO, I. F.; GREGO, C. R.; CRUZ, P. G. da; MARTINS, D. N.; SILVA, J. das D. B. da; OLIVEIRA, A. M. de; RODRIGUES, K. C. L. |
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Afiliação: |
JULIANA ALVES DIAS, CPAF-RO; IVANETE FRANCESCHINI PACHECO, Universidade Federal de Rondônia; CELIA REGINA GREGO, CNPTIA; PEDRO GOMES DA CRUZ, CPAF-RO; DHIELSON NAVAS MARTINS, Faculdades Integradas Aparício Carvalho; JOSILUCE DAS DORES BONFIM DA SILVA, Faculdades Integradas Aparício Carvalho; AUDENICE MIRANDA DE OLIVEIRA, Universidade Federal de Rondônia; KAREN CRISTINA LOEBLEIN RODRIGUES, Faculdades Integradas Aparício Carvalho. |
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Título: |
Avaliação espacial da composição química do leite cru refrigerado de três microrregiões do estado de Rondônia. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
In: CONGRESSO INTERNACIONAL DE PECUÁRIA LEITEIRA DA AMAZÔNIA OCIDENTAL, 1., 2019, Rio Branco, AC. Anais... Rio Branco, AC: Edufac, 2019. |
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Páginas: |
p. 34-35. |
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ISBN: |
978-85-8236-112-2 |
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Idioma: |
Português |
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Conteúdo: |
Considerando a escassez de informações sobre a qualidade composicional do leite cru refrigerado em Rondônia, o objetivo deste trabalho foi avaliar a composição química do leite cru armazenado em tanques de resfriamento e identificar aglomerados espaciais destes indicadores visando a identificação de áreas prioritárias de atuação. |
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Palavras-Chave: |
Krigagem; Qualidade do leite; Sazonalidade; Tanques de resfriamento. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01243nam a2200265 a 4500
001 2118499
005 2020-01-29
008 2019 bl uuuu u01u1 u #d
020 $a978-85-8236-112-2
100 1 $aDIAS, J. A.
245 $aAvaliação espacial da composição química do leite cru refrigerado de três microrregiões do estado de Rondônia.$h[electronic resource]
260 $aIn: CONGRESSO INTERNACIONAL DE PECUÁRIA LEITEIRA DA AMAZÔNIA OCIDENTAL, 1., 2019, Rio Branco, AC. Anais... Rio Branco, AC: Edufac$c2019
300 $ap. 34-35.
520 $aConsiderando a escassez de informações sobre a qualidade composicional do leite cru refrigerado em Rondônia, o objetivo deste trabalho foi avaliar a composição química do leite cru armazenado em tanques de resfriamento e identificar aglomerados espaciais destes indicadores visando a identificação de áreas prioritárias de atuação.
653 $aKrigagem
653 $aQualidade do leite
653 $aSazonalidade
653 $aTanques de resfriamento
700 1 $aPACHECO, I. F.
700 1 $aGREGO, C. R.
700 1 $aCRUZ, P. G. da
700 1 $aMARTINS, D. N.
700 1 $aSILVA, J. das D. B. da
700 1 $aOLIVEIRA, A. M. de
700 1 $aRODRIGUES, K. C. L.
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