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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
14/05/1996 |
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Data da última atualização: |
14/05/1996 |
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Autoria: |
SAMPAIO, M. J. A. M. |
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Título: |
Studies on Cyanobacterial glutamine synthetase. |
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Ano de publicação: |
1979 |
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Fonte/Imprenta: |
Dundee: University of Dundee, Agosto, 1979. |
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Páginas: |
161p. |
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Série: |
Tese de Doutorado. |
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Idioma: |
Inglês |
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Notas: |
Orientada por: W.D.P. Stwart. |
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Conteúdo: |
Glutamine synthetase has been purified to homogeneity from three cyanobacteria: Anabaena cylindrica, a species of Nostoc (isolated from the lichen Peltigera canina) and Plectonema boryanum. The activities of the A. cylindrica, Nostoc sp and P. boryanum enzymes in the biosynthetic assay were, respectively, 9.4, 6.5 and 5.0 umol product formed. mg protein -1.min-1. Stabilization of the enzyme required Mg2+, glutamate, EDTA and a thiol reagent to be present during purification. The molecular weight of the A. cylindrica enzyme was 591,000 as estimated by sedimentation analysis, 660,000 by gel filtration and 565,000 by polyacrylamide gel electrophoresis; the Nostoc sp and P. boryanum enzymes gave values in the same region. The molecular weights of the sub-units ofeach enzyme were approximately 50,000. Electron microscopy revealed that each molecule was composed of 12 sub-units arranged in two superimposed hexagonal rings. When dialysed in the absence of stabilizing ligands the A. cylindrica enzyme lost activity and the protein band characteristic of the native enzyme was replaced by three bands with approximate molecular weights of 510,000, 310,000, and 130,000. These sub-species re-associated and activity was restored by adding 2-mercaptoethanol and substrates. The Nostoc sp and P. boryanum enzymes did not show such deactivation.Some kinetic and regulatory properties of the purified A. cylindrica, Nostoc and P. boryanum glutamine synthetases were determined. The feed-back inhibition pattern observed with.............. MenosGlutamine synthetase has been purified to homogeneity from three cyanobacteria: Anabaena cylindrica, a species of Nostoc (isolated from the lichen Peltigera canina) and Plectonema boryanum. The activities of the A. cylindrica, Nostoc sp and P. boryanum enzymes in the biosynthetic assay were, respectively, 9.4, 6.5 and 5.0 umol product formed. mg protein -1.min-1. Stabilization of the enzyme required Mg2+, glutamate, EDTA and a thiol reagent to be present during purification. The molecular weight of the A. cylindrica enzyme was 591,000 as estimated by sedimentation analysis, 660,000 by gel filtration and 565,000 by polyacrylamide gel electrophoresis; the Nostoc sp and P. boryanum enzymes gave values in the same region. The molecular weights of the sub-units ofeach enzyme were approximately 50,000. Electron microscopy revealed that each molecule was composed of 12 sub-units arranged in two superimposed hexagonal rings. When dialysed in the absence of stabilizing ligands the A. cylindrica enzyme lost activity and the protein band characteristic of the native enzyme was replaced by three bands with approximate molecular weights of 510,000, 310,000, and 130,000. These sub-species re-associated and activity was restored by adding 2-mercaptoethanol and substrates. The Nostoc sp and P. boryanum enzymes did not show such deactivation.Some kinetic and regulatory properties of the purified A. cylindrica, Nostoc and P. boryanum glutamine synthetases were determined. The feed-back inhib... Mostrar Tudo |
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Palavras-Chave: |
Cianobactéria; Cyanophyta; Glutamine sythetase; Purification. |
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Thesagro: |
Bactéria; Enzima; Extração; Purificação. |
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Thesaurus Nal: |
analytical methods; enzymes. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02230nam a2200265 a 4500
001 1621455
005 1996-05-14
008 1979 bl uuuu m 00u1 u #d
100 1 $aSAMPAIO, M. J. A. M.
245 $aStudies on Cyanobacterial glutamine synthetase.
260 $aDundee: University of Dundee, Agosto$c1979
300 $a161p.
490 $aTese de Doutorado.
500 $aOrientada por: W.D.P. Stwart.
520 $aGlutamine synthetase has been purified to homogeneity from three cyanobacteria: Anabaena cylindrica, a species of Nostoc (isolated from the lichen Peltigera canina) and Plectonema boryanum. The activities of the A. cylindrica, Nostoc sp and P. boryanum enzymes in the biosynthetic assay were, respectively, 9.4, 6.5 and 5.0 umol product formed. mg protein -1.min-1. Stabilization of the enzyme required Mg2+, glutamate, EDTA and a thiol reagent to be present during purification. The molecular weight of the A. cylindrica enzyme was 591,000 as estimated by sedimentation analysis, 660,000 by gel filtration and 565,000 by polyacrylamide gel electrophoresis; the Nostoc sp and P. boryanum enzymes gave values in the same region. The molecular weights of the sub-units ofeach enzyme were approximately 50,000. Electron microscopy revealed that each molecule was composed of 12 sub-units arranged in two superimposed hexagonal rings. When dialysed in the absence of stabilizing ligands the A. cylindrica enzyme lost activity and the protein band characteristic of the native enzyme was replaced by three bands with approximate molecular weights of 510,000, 310,000, and 130,000. These sub-species re-associated and activity was restored by adding 2-mercaptoethanol and substrates. The Nostoc sp and P. boryanum enzymes did not show such deactivation.Some kinetic and regulatory properties of the purified A. cylindrica, Nostoc and P. boryanum glutamine synthetases were determined. The feed-back inhibition pattern observed with..............
650 $aanalytical methods
650 $aenzymes
650 $aBactéria
650 $aEnzima
650 $aExtração
650 $aPurificação
653 $aCianobactéria
653 $aCyanophyta
653 $aGlutamine sythetase
653 $aPurification
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria Tropical. Para informações adicionais entre em contato com cnpat.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria Tropical. |
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Data corrente: |
28/05/2021 |
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Data da última atualização: |
30/06/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
ALVES FILHO, E. G.; SILVA, L. M. A. e; BRITO, E. S. de; CASTRO, D. R. G.; BEZERRA, J. A.; SANCHES, E. A.; RODRIGUES, S.; FERNANDES, F. A. N.; CAMPELO, P. H. |
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Afiliação: |
ELENILSON G. ALVES FILHO, Departamento de Tecnologia de Alimentos (DETAL), Universidade Federal do Ceará; LORENA MARA ALEXANDRE E SILVA, CNPAT; EDY SOUSA DE BRITO, CNPAT; DÉBORA R. G. CASTRO, Grupo de Inovação em Biotecnologia e Alimentos da Amazônia (gIBA), Universidade Federal do Amazonas; JAQUELINE A. BEZERRA, Instituto Federal de Educação, Ciência e Tecnologia do Amazonas; EDGAR A. SANCHES, Laboratório de Polímeros Nanoestruturados (NANOPOL), Universidade Federal do Amazonas; SUELI RODRIGUES, Departamento de Tecnologia de Alimentos (DETAL), Universidade Federal do Ceará; FABIANO A. N. FERNANDES, Departamento de Engenharia Química (DEQ), Universidade Federal do Ceará; PEDRO HENRIQUE CAMPELO, Grupo de Inovação em Biotecnologia e Alimentos da Amazônia (gIBA), Universidade Federal do Amazonas. |
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Título: |
Effect of glow and dielectric barrier discharges plasma on volatile and non-volatile chemical profiling of camu-camu juice. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Food and Bioprocess Technology, April, 2021. |
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DOI: |
https://doi.org/10.1007/s11947-021-02639-6 |
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Idioma: |
Inglês |
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Palavras-Chave: |
Atmospheric cold plasma; Glow discharge plasma; Plasma de descarga brilhante; Plasma frio atmosférico; Quimiometria. |
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Thesagro: |
Myrciaria Dubia. |
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Thesaurus NAL: |
Chemometrics. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00978naa a2200301 a 4500
001 2132083
005 2021-06-30
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11947-021-02639-6$2DOI
100 1 $aALVES FILHO, E. G.
245 $aEffect of glow and dielectric barrier discharges plasma on volatile and non-volatile chemical profiling of camu-camu juice.$h[electronic resource]
260 $c2021
650 $aChemometrics
650 $aMyrciaria Dubia
653 $aAtmospheric cold plasma
653 $aGlow discharge plasma
653 $aPlasma de descarga brilhante
653 $aPlasma frio atmosférico
653 $aQuimiometria
700 1 $aSILVA, L. M. A. e
700 1 $aBRITO, E. S. de
700 1 $aCASTRO, D. R. G.
700 1 $aBEZERRA, J. A.
700 1 $aSANCHES, E. A.
700 1 $aRODRIGUES, S.
700 1 $aFERNANDES, F. A. N.
700 1 $aCAMPELO, P. H.
773 $tFood and Bioprocess Technology, April, 2021.
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