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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
27/12/2000 |
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Data da última atualização: |
27/12/2000 |
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Autoria: |
SALLES, J. F.; GITAHY, P. de M.; SKOT, L.; BALDANI, J. I. |
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Título: |
Use of endophytic diazotrophic bacteria as a vector to express the cry3A from Bacillus thuringiensis. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, Brasilia, v. 31, p. 155-161, 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
The goal of this study was to evaluate the potential of endophytic diazotrophic bacteria as a vector to express a cry gene from Bacillus thuringiensis, envisaging the control of pests that attack sugarcane plants. The endophytic nitrogen-fixing bacteria Gluconacetobacter diazotrophicus strain BR11281 and Herbaspirillum seropedicae strain BR11335 were used as models. The cry3A gene was transferred by conjugation using a suicide plasmid and the recombinant strains were selected by their ability to fix nitrogen in semi-solid N-free medium. The presence of the cry gene was detected by Southem-blot using an intemal fragment of 1.0 kb as a probe. The production of 8-endotoxin by the recombinant H. seropedicae strain was detected by dot blot while for G. diazotrophicus the Westem-blot technique was used. In both cases, a specific antibody raised against the B. thuringiensis toxin was applied. The 8-endotoxin production showed by the G. diazotrophicus recombinant strain was dependent on the nitrogen fixing conditions since the cry3A gene was fused to a nif promoter. In the case of H. seropedicae the 8-endotoxin expression was not affected by the promoter (rhi) used. These results suggest that endophytic diazotrophic bacteria can be used as vectors to express entomopathogenic genes envisaging control of sugarcane pests.
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Palavras-Chave: |
Bactéria diazotrófica endofítica; Endophytic diazotrophy bacteria. |
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Thesagro: |
Bacillus Thuringiensis. |
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Thesaurus Nal: |
Gluconacetobacter diazotrophicus; Herbaspirillum seropedicae. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02046naa a2200217 a 4500
001 1597290
005 2000-12-27
008 2000 bl --- 0-- u #d
100 1 $aSALLES, J. F.
245 $aUse of endophytic diazotrophic bacteria as a vector to express the cry3A from Bacillus thuringiensis.
260 $c2000
520 $aThe goal of this study was to evaluate the potential of endophytic diazotrophic bacteria as a vector to express a cry gene from Bacillus thuringiensis, envisaging the control of pests that attack sugarcane plants. The endophytic nitrogen-fixing bacteria Gluconacetobacter diazotrophicus strain BR11281 and Herbaspirillum seropedicae strain BR11335 were used as models. The cry3A gene was transferred by conjugation using a suicide plasmid and the recombinant strains were selected by their ability to fix nitrogen in semi-solid N-free medium. The presence of the cry gene was detected by Southem-blot using an intemal fragment of 1.0 kb as a probe. The production of 8-endotoxin by the recombinant H. seropedicae strain was detected by dot blot while for G. diazotrophicus the Westem-blot technique was used. In both cases, a specific antibody raised against the B. thuringiensis toxin was applied. The 8-endotoxin production showed by the G. diazotrophicus recombinant strain was dependent on the nitrogen fixing conditions since the cry3A gene was fused to a nif promoter. In the case of H. seropedicae the 8-endotoxin expression was not affected by the promoter (rhi) used. These results suggest that endophytic diazotrophic bacteria can be used as vectors to express entomopathogenic genes envisaging control of sugarcane pests.
650 $aGluconacetobacter diazotrophicus
650 $aHerbaspirillum seropedicae
650 $aBacillus Thuringiensis
653 $aBactéria diazotrófica endofítica
653 $aEndophytic diazotrophy bacteria
700 1 $aGITAHY, P. de M.
700 1 $aSKOT, L.
700 1 $aBALDANI, J. I.
773 $tBrazilian Journal of Microbiology, Brasilia$gv. 31, p. 155-161, 2000.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 14 | |
| 10. |  | CANUTO, E. de L.; SALLES, J. F.; OLIVEIRA, A. L. M.; PERIN, L.; REIS, V. M.; BALDANI, J. I. Respostas de plantas micropropagadas de cana-de-açúcar à inoculação de bactérias diazotróficas endofíticas. Agronomia, Seropédica, RJ, v. 37, n. 2, p. 67-72, ago./dez. 2003. Response of micropropagated sugarcane plants to inoculation with endophytic diazotrophic bacteria.| Biblioteca(s): Embrapa Agrobiologia. |
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| 11. | | BRASIL, M. S.; RODRIGUES, L. S.; SALLES, J. F.; BALDANI, V. L. D.; REIS, V. M.; BALDANI, J. I. Uso de "primers" espécie-específicos para identificação e detecção no ambiente de bactérias fixadoras de N2 do gênero Burkholderia spp. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 22., 2003, Florianópolis. Resumos... Florianópolis: SBM, 2003. 1 p.| Biblioteca(s): Embrapa Agrobiologia. |
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| 12. | | DIAS, A. C. F.; SILVA, M. C. P. e; SALLES, J. F.; AZEVEDO, J. L.; MELO, I. S. de; ELSAS, J. D. van; ANDREOTE, F. D. Linking the edges of microbially-mediated nitrogen transformations in mangrove sediments. In: SYMPOSIUM ON BACTERIAL GENETICS AND ECOLOGY - BAGECO 2011, 11., 2011, Corfu. Book of Abstracts... Corfu: University of Athens, 2011. P2.06.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 13. | | REIS, V. M.; CRUZ, G. B.; FERREIRA, A.; FERNANDES, M. F.; FERREIRA, A. C.; REIS, F. B.; RIBEIRO, J. R. A.; SALLES, J. F.; WEBER, O. B. Produção e caracterização de soros policlonais para a detecção de bactérias diazotróficas. Seropédica: EMBRAPA-CNPAB, 1997. 11 p. (EMBRAPA-CNPAB. Documentos, 30).| Biblioteca(s): Embrapa Agrobiologia. |
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| 14. | | VIDEIRA, S. S.; SILVA, M. de C. P. e; GALISA, P. de S.; DIAS, A. C. F.; NISSINEM R.; BALDANI, V. L. D.; van ELSAS, J. D.; BALDANI, J. I.; SALLES, J. F. Culture-independent molecular approaches reveal a mostly unknown high diversity of active nitrogen-fixing bacteria associated with Pennisetum purpureum: a bionergy crop. Plant and Soil, v. 373, p. 737-754, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 14 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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