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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
01/12/1992 |
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Data da última atualização: |
01/12/1992 |
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Autoria: |
MESHI, T.; WATANABE, Y.; SAITO, T.; SUGIMOTO, A.; MAEDA, T.; OKADA, Y. |
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Afiliação: |
Faculty of Science, University of Tokyo, Hongo, Tokyo 113, Japan. |
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Título: |
Function of the 30 kd protein of tobacco mosaic virus: involvement in cell-to-cell movement and dispensability for replication. |
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Ano de publicação: |
1987 |
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Fonte/Imprenta: |
The Embo Journal, v.6, n.9, p.2557-2563, 1987. |
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Idioma: |
Inglês |
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Conteúdo: |
We have investigated the function of the 30 kd protein of tobacco mosaic virus (TMV) by a reserve genetics approach. First, a point mutation of TMV Ls1 (a temperature-sensitive mutant defective in cell-to-cell movement), that causes an amino acid substitution in the 30 kd protein, was introduced into the parent strain, TMV L. The generated mutant showed the same phenotype as TMV Ls1, and therefore the one-base substitution in the 30 kd protein gene adequately explains the defectiveness of TMV Ls1. Next, four kinds of frame-shift mutants were constructed, whose mutations are located at three different positions of the 30 kd protein gene. All the frame-shift mutants were replication-competent in protoplasts but none showed infectivity on tobacco plants. From these observations the 30 kd protein was confirmed to be involved in cell-to-cell movement. To clarify that the 30 kd protein is not necessary for replication, two kinds of deletion mutants were construted; one lacking most of the 30 kd protein gene and the other lacking both the 30 kd and coat protein genes. Both mutants replicated in protoplasts and the former still produced the subgenomic mRNA for the coat protein. These results clearly showed that the 30 kd protein, as well as the coat protein, is dispensable for replication and thar no cisacting element for replication is located in their coding sequences. It is also suggested that the signal for coat protein mRNA synthesis may be located within about 100 nucleotides upstream of the initiation codon of the c MenosWe have investigated the function of the 30 kd protein of tobacco mosaic virus (TMV) by a reserve genetics approach. First, a point mutation of TMV Ls1 (a temperature-sensitive mutant defective in cell-to-cell movement), that causes an amino acid substitution in the 30 kd protein, was introduced into the parent strain, TMV L. The generated mutant showed the same phenotype as TMV Ls1, and therefore the one-base substitution in the 30 kd protein gene adequately explains the defectiveness of TMV Ls1. Next, four kinds of frame-shift mutants were constructed, whose mutations are located at three different positions of the 30 kd protein gene. All the frame-shift mutants were replication-competent in protoplasts but none showed infectivity on tobacco plants. From these observations the 30 kd protein was confirmed to be involved in cell-to-cell movement. To clarify that the 30 kd protein is not necessary for replication, two kinds of deletion mutants were construted; one lacking most of the 30 kd protein gene and the other lacking both the 30 kd and coat protein genes. Both mutants replicated in protoplasts and the former still produced the subgenomic mRNA for the coat protein. These results clearly showed that the 30 kd protein, as well as the coat protein, is dispensable for replication and thar no cisacting element for replication is located in their coding sequences. It is also suggested that the signal for coat protein mRNA synthesis may be located within about 100 nucleotides ... Mostrar Tudo |
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Palavras-Chave: |
30k protein; 30k proteina; Efeito; Transport effect. |
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Thesagro: |
Transporte; Vírus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02232naa a2200253 a 4500
001 1457955
005 1992-12-01
008 1987 bl --- 0-- u #d
100 1 $aMESHI, T.
245 $aFunction of the 30 kd protein of tobacco mosaic virus$binvolvement in cell-to-cell movement and dispensability for replication.
260 $c1987
520 $aWe have investigated the function of the 30 kd protein of tobacco mosaic virus (TMV) by a reserve genetics approach. First, a point mutation of TMV Ls1 (a temperature-sensitive mutant defective in cell-to-cell movement), that causes an amino acid substitution in the 30 kd protein, was introduced into the parent strain, TMV L. The generated mutant showed the same phenotype as TMV Ls1, and therefore the one-base substitution in the 30 kd protein gene adequately explains the defectiveness of TMV Ls1. Next, four kinds of frame-shift mutants were constructed, whose mutations are located at three different positions of the 30 kd protein gene. All the frame-shift mutants were replication-competent in protoplasts but none showed infectivity on tobacco plants. From these observations the 30 kd protein was confirmed to be involved in cell-to-cell movement. To clarify that the 30 kd protein is not necessary for replication, two kinds of deletion mutants were construted; one lacking most of the 30 kd protein gene and the other lacking both the 30 kd and coat protein genes. Both mutants replicated in protoplasts and the former still produced the subgenomic mRNA for the coat protein. These results clearly showed that the 30 kd protein, as well as the coat protein, is dispensable for replication and thar no cisacting element for replication is located in their coding sequences. It is also suggested that the signal for coat protein mRNA synthesis may be located within about 100 nucleotides upstream of the initiation codon of the c
650 $aTransporte
650 $aVírus
653 $a30k protein
653 $a30k proteina
653 $aEfeito
653 $aTransport effect
700 1 $aWATANABE, Y.
700 1 $aSAITO, T.
700 1 $aSUGIMOTO, A.
700 1 $aMAEDA, T.
700 1 $aOKADA, Y.
773 $tThe Embo Journal$gv.6, n.9, p.2557-2563, 1987.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 24 | |
| 5. |  | BICUDO, S. D.; AZEVEDO, H. C.; MAIA, M. S. S.; SOUSA, D. B.; RODELLO, L. Aspectos peculiares da inseminação artificial em ovinos. Acta Scientiae Veterinariae, Porto Alegre, v. 33, supl 1, p. 127-130, 2005.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 6. | | MAUES, M. M.; LEÃO, N. V. M.; SOUSA, D. B. de. Fenologia reprodutiva de especies arbustivas e arbóreas de vegetação secundária em duas áreas de diferentes idades, no município de Igarapé-Açu (PA). In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA CNPq/FCAP/EMBRAPA, 6., 1996, Belém, PA. Resumos. Belém, PA: FCAP, 1996. p. 85, 151. Publicado também em Congresso Brasileiro de Iniciação Científica em Ciências Agrárias, 16., 1996, Santa Maria, RS.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 7. | | SOUSA, D. B. de; MAUES, M. M.; LEÃO, N. V. M. Fenologia reprodutiva de espécies arbustivas e arbóreas em vegetação secundária no Nordeste paraense. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA, 5., 1995, Belém, PA. Resumos... Belém, PA: FCAP, Unidade de Apoio à Pesquisa, 1995. p. 58, 148.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 9. | | MAIA, M. S.; BICUDO, S. D.; AZEVEDO, H. C.; SICHERLE, C. C; SOUSA, D. B.; RODELLO, L. Geração de metabólitos reativos do oxigênio (ROS) em diluentes para a criopreservação de sêmen. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 17., 2007, . Anais... Belo Horizonte: CBRA, 2007. p. 198| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 10. | | SOUSA, D. B. A. de; SILVA, K. J. D. e; ROCHA, M. de M.; MENEZES JUNIOR, J. A. de. Competição de linhagens de feijão-caupi, subclasse fradinho, em Teresina, PI. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA MEIO-NORTE, 3., 2017, Teresina, PI. Anais da III Jornada Científica da Embrapa Meio-Norte. Teresina: Embrapa Meio-Norte, 2017. p. 40.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 11. | | SOUSA, D. B.; BICUDO, S. D.; MANZATO, A. J.; AZEVEDO, H. C.; MAIA, M. S.; RODELLO, L.; SICHERLE, C. C. Ranqueamento qualitativo do sêmen congelado de carneiros através da análise estatística multivariada avaliado pelo sistema Casa e sondas fluorescentes. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 17., 2007, Curitiba. Anais... Belo Horizonte: CBRA, 2007. p. 148.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 14. | | AZEVEDO, H. C.; BICUDO, S. D.; MAIA, M. da S.; SOUSA, D. B.; RODELLO, L.; SICHERLE, C. C. Influência da crioresistência da membrana plasmática sobre a avaliação computadorizada ma motilidade de espermatozóides ovinos criopreservados. In: SIMPÓSIO INTERNACIONAL SOBRE CAPRINOS E OVINOS DE CORTE, 3., 2007, João Pessoa. Anais... João Pessoa, 2007. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 17. | | MAIA, M. da S.; BICUDO, S. D.; AZEVEDO, H. C.; SICHERLE, C. C.; SOUSA, D. B. de; RODELLO, L. Molity and viability of ram sperm cryopreserved in a Tris-egg yolk extender supplemented with anti-oxidants. Small Ruminant Research, Ney Yourk, v. 85, p. 85-90, 2009. Artigo de periódico.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 18. | | AZEVEDO, H. C.; BICUDO, S. D.; SOUSA, D. B.; MAIA, M. da S.; RODELLO, L.; SICHERLE, C. C. Measurement of sperm capacitation and acrosome reaction - like changes by chlortetracycline test (CTC) can predict the freezability of ram semen? Reproduction in Domestic Animals, Berlin, v. 43, n. 3, p. 70, 2008. Supplement, ref. P128. Edição dos Abstracts do 16. International Congress on Animal Reproduction, Budapest, jul. 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 19. | | AZEVEDO, H. C.; BICUDO, S D.; SOUSA, D. B.; MAIA, M. S.; RODELLO, L.; SICHERLE, C. C. Measurement of sperm capacitation and acrosome reaction - like changes by chortetracycline test (CTC) can predict the freezability of ram semn? In: INTERNATIONAL CONGRESS ON ANIMAL REPRODUCTION, 16., 2008, Budapest. Abstracts... European Society for Domestic Animal Reproduction, 2008. Resumo em anais.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| Registros recuperados : 24 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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