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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
13/04/2004 |
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Data da última atualização: |
27/08/2018 |
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Autoria: |
GOMES, E. A.; BELICUAS, S. N. J.; GUIMARÃES, C. T.; SOUZA, I. R. P. de; SABATO, E. de O. |
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Afiliação: |
ELIANE APARECIDA GOMES, CNPMS; SILVIA NETO JARDIM BELICUAS, CNPMS; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; ISABEL REGINA PRAZERES DE SOUZA, CNPMS; ELIZABETH DE OLIVEIRA SABATO, CNPMS. |
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Título: |
Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plants. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 39, n. 1, p. 61-65, jan. 2004 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Variabilidade genética de fitoplasma e espiroplasma isolados de plantas de milho no Brasil. |
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Conteúdo: |
The objective of this work was to characterize the genetic variability of phytoplasma and Spiroplasma kunkelii isolated from maize plants showing symptoms of stunt collected from different Brazilian geographic regions. A DNA fragment of 500 base pairs (bp) was amplified from the spiralin gene in S. kunkelii and one fragment of 1,200 bp was generated from 16S rDNA gene in phytoplasma. The partial sequences of the spiralin gene showed similarity of 98% among the isolates of S. kunkelii analyzed. These sequences were compared with the sequence of the spiralin gene from other Spiroplasma species deposited in the GenBank, resulting in a similarity varying from 76.9% to 88.1%. The 16S rDNA sequence from the phytoplasma were completely similar within the Brazilian isolates and showed up to 98% of the similarity with sequences already found from other phytoplasmas. A very narrow genetic variability was detected by these gene fragments within phytoplasma and Spiroplasma analyzed. However, other genomic regions with higher polymorphic levels shall be identified in order to better evaluate the genetic diversity within these microorganisms population. |
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Palavras-Chave: |
espiralina; gene sequence; molicute; seqüência genética; spiralin. |
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Thesagro: |
Microrganismo; Polimorfismo Genético; Zea Mays. |
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Thesaurus Nal: |
genetic polymorphism; microorganisms; Mollicutes. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/26120/1/v39n01a09.pdf
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Marc: |
LEADER 02153naa a2200313 a 4500
001 1109810
005 2018-08-27
008 2004 bl uuuu u00u1 u #d
100 1 $aGOMES, E. A.
245 $aGenetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plants.
260 $c2004
500 $aTítulo em português: Variabilidade genética de fitoplasma e espiroplasma isolados de plantas de milho no Brasil.
520 $aThe objective of this work was to characterize the genetic variability of phytoplasma and Spiroplasma kunkelii isolated from maize plants showing symptoms of stunt collected from different Brazilian geographic regions. A DNA fragment of 500 base pairs (bp) was amplified from the spiralin gene in S. kunkelii and one fragment of 1,200 bp was generated from 16S rDNA gene in phytoplasma. The partial sequences of the spiralin gene showed similarity of 98% among the isolates of S. kunkelii analyzed. These sequences were compared with the sequence of the spiralin gene from other Spiroplasma species deposited in the GenBank, resulting in a similarity varying from 76.9% to 88.1%. The 16S rDNA sequence from the phytoplasma were completely similar within the Brazilian isolates and showed up to 98% of the similarity with sequences already found from other phytoplasmas. A very narrow genetic variability was detected by these gene fragments within phytoplasma and Spiroplasma analyzed. However, other genomic regions with higher polymorphic levels shall be identified in order to better evaluate the genetic diversity within these microorganisms population.
650 $agenetic polymorphism
650 $amicroorganisms
650 $aMollicutes
650 $aMicrorganismo
650 $aPolimorfismo Genético
650 $aZea Mays
653 $aespiralina
653 $agene sequence
653 $amolicute
653 $aseqüência genética
653 $aspiralin
700 1 $aBELICUAS, S. N. J.
700 1 $aGUIMARÃES, C. T.
700 1 $aSOUZA, I. R. P. de
700 1 $aSABATO, E. de O.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 39, n. 1, p. 61-65, jan. 2004
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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| Registros recuperados : 65 | |
| 2. |  | SABATO, E. de O. Enfezamento do milho. In: OLIVEIRA, C. M. de; SABATO, E. de O. (Ed.). Doenças em milho: insetos-vetores, molicutes e vírus. Brasília, DF: Embrapa, 2017. cap. 1, p. 11-24. il. color. Texto em português e inglês. Título equivalente: Corn stunting diseases.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 4. | | SABATO, E. de O. Enfezamentos e viroses no milho. In: SEMINÁRIO NACIONAL [DE] MILHO SAFRINHA, 14., 2017, Cuiabá. Construindo sistemas de produção sustentáveis e rentáveis: livro de palestras. Sete Lagoas: Associação Brasileira de Milho e Sorgo, 2017. cap. 7, p. 196-219. Editora técnica: Maria Cristina Dias Paes.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 65 | |
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