| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Café. |
|
Data corrente: |
13/04/2011 |
|
Data da última atualização: |
13/04/2011 |
|
Tipo da produção científica: |
Artigo em Anais de Congresso |
|
Autoria: |
FRANCO, O. L.; ANDRADE, A. E.; BARROS, E. V. S. A.; SA, M. F. G. de; CARNEIRO, R. M. D. G.; CARNEIRO, R.; EIRA, M. T. S. da; ROCHA, T. L.; REIS, A. M. dos. |
|
Afiliação: |
UNIVERSIDADE CATÓLICA DE BRASÍLIA; MARIA FATIMA GROSSI DE SA, CENARGEN; REGINA MARIA DECHECHI G CARNEIRO, CENARGEN; IAPAR; MIRIAN THEREZINHA SOUZA DA EIRA, SAPC; THALES LIMA ROCHA, CENARGEN; ANGELA MEHTA DOS REIS, CENARGEN. |
|
Título: |
Comparison of different staining methods for coffee proteomic analysis. |
|
Ano de publicação: |
2007 |
|
Fonte/Imprenta: |
In:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Proteomic methods, such as bidimensional electrophoresis (2-DE) and mass spectrometry, have been extensively used for the study of protein differential expression in several plants including Arabidopsis thaliana, rice and wheat. Specifically in the 2-DE method, deep attention must be given to the protein staining technique, which often involves silver nitrate or Coomassie Brilliant Blue (CBB). Silver staining is usually preferred over CBB due to the higher sensitivity obtained. Nevertheless, silver-staining resolution could significantly vary according to the studied organism and more specifically to the researched tissue. In Coffea spp., 2-DE analysis has been rarely employed. Some studies of protein expression have been reported in this culture mainly involving the biosynthesis of caffeine and metabolism during seed germination. The study of the global protein expression in coffee plants in response to biotic stress conditions had not been reported until now. Phytonematode infection can be considered one of the most important biotic stresses that affect coffee production and Meloidogyne paranaensis is one of the major nematode species that infects coffee plants. In this report, the protein expression of infected- and non-infected roots of coffee (Coffea canephora) were analyzed and the protein pattern determined by 2-DE. Gels were stained with silver nitrate or CBB, in order to obtain an optimized method for proteomic analysis of plant-nematode interaction. The 2-DE analysis revealed an enhanced number of protein spots, as well as differentially expressed proteins, when CBB was used. A total of approximately 70 and 100 spots were observed in silver and CBB stained gels, respectively. Moreover, 18 differentially expressed proteins were observed in CBB gels, and only 8 in the silver stained gels. This report showed that the staining method was crucial for an optimized protein analysis of coffee. Similar results were obtained for cotton roots and therefore these results may be extended to other plant species in order to better understand the host-pathogen interaction. MenosProteomic methods, such as bidimensional electrophoresis (2-DE) and mass spectrometry, have been extensively used for the study of protein differential expression in several plants including Arabidopsis thaliana, rice and wheat. Specifically in the 2-DE method, deep attention must be given to the protein staining technique, which often involves silver nitrate or Coomassie Brilliant Blue (CBB). Silver staining is usually preferred over CBB due to the higher sensitivity obtained. Nevertheless, silver-staining resolution could significantly vary according to the studied organism and more specifically to the researched tissue. In Coffea spp., 2-DE analysis has been rarely employed. Some studies of protein expression have been reported in this culture mainly involving the biosynthesis of caffeine and metabolism during seed germination. The study of the global protein expression in coffee plants in response to biotic stress conditions had not been reported until now. Phytonematode infection can be considered one of the most important biotic stresses that affect coffee production and Meloidogyne paranaensis is one of the major nematode species that infects coffee plants. In this report, the protein expression of infected- and non-infected roots of coffee (Coffea canephora) were analyzed and the protein pattern determined by 2-DE. Gels were stained with silver nitrate or CBB, in order to obtain an optimized method for proteomic analysis of plant-nematode interaction. The 2-DE analys... Mostrar Tudo |
|
Palavras-Chave: |
Bidimensional electrophoresis; Proteomic method. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32592/1/Comparison-of-Different.pdf
|
|
Marc: |
LEADER 02933nam a2200229 a 4500
001 1885752
005 2011-04-13
008 2007 bl uuuu u00u1 u #d
100 1 $aFRANCO, O. L.
245 $aComparison of different staining methods for coffee proteomic analysis.$h[electronic resource]
260 $aIn:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM.$c2007
520 $aProteomic methods, such as bidimensional electrophoresis (2-DE) and mass spectrometry, have been extensively used for the study of protein differential expression in several plants including Arabidopsis thaliana, rice and wheat. Specifically in the 2-DE method, deep attention must be given to the protein staining technique, which often involves silver nitrate or Coomassie Brilliant Blue (CBB). Silver staining is usually preferred over CBB due to the higher sensitivity obtained. Nevertheless, silver-staining resolution could significantly vary according to the studied organism and more specifically to the researched tissue. In Coffea spp., 2-DE analysis has been rarely employed. Some studies of protein expression have been reported in this culture mainly involving the biosynthesis of caffeine and metabolism during seed germination. The study of the global protein expression in coffee plants in response to biotic stress conditions had not been reported until now. Phytonematode infection can be considered one of the most important biotic stresses that affect coffee production and Meloidogyne paranaensis is one of the major nematode species that infects coffee plants. In this report, the protein expression of infected- and non-infected roots of coffee (Coffea canephora) were analyzed and the protein pattern determined by 2-DE. Gels were stained with silver nitrate or CBB, in order to obtain an optimized method for proteomic analysis of plant-nematode interaction. The 2-DE analysis revealed an enhanced number of protein spots, as well as differentially expressed proteins, when CBB was used. A total of approximately 70 and 100 spots were observed in silver and CBB stained gels, respectively. Moreover, 18 differentially expressed proteins were observed in CBB gels, and only 8 in the silver stained gels. This report showed that the staining method was crucial for an optimized protein analysis of coffee. Similar results were obtained for cotton roots and therefore these results may be extended to other plant species in order to better understand the host-pathogen interaction.
653 $aBidimensional electrophoresis
653 $aProteomic method
700 1 $aANDRADE, A. E.
700 1 $aBARROS, E. V. S. A.
700 1 $aSA, M. F. G. de
700 1 $aCARNEIRO, R. M. D. G.
700 1 $aCARNEIRO, R.
700 1 $aEIRA, M. T. S. da
700 1 $aROCHA, T. L.
700 1 $aREIS, A. M. dos
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Café (CNPCa) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 249 | |
| 141. |  | CORREZZI, A. C.; DIAS, S. C.; FRANCO, O. L.; BATISTA, C. L.; RAMOS, C. S.; GOMES, D. G.; SILVA, O. N.; MIGLIOLIO, L.; SA, M. F. G. de; OLIVEIRA, U. A.; SILVA, M. C. M. da. Biotechnological potential of the brazilian cerrado plants: investigating the inhibition of insect alpha amylases. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 146.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|   |
| 142. | | NAKASU, E. Y. T.; DIAS, S. C.; PIRES, C. S. S.; ANDOW, D. A.; PAULA, D. P.; TOGNI, P. H. B.; MACEDO, T. R.; SUJII, E. R.; SÁ, M. F. G. de; FONTES, E. M. G. Bitrophic toxicity of Cry1Ac to Cycloneda sanguinea, a predator in Brazilian cotton. Entomologia Experimentalis et Applicata, v. 148, p. 105-115, 2013| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|  |
| 143. | | ROCHA, T. L.; BOUGHTON, B. A.; SIQUEIRA, T.; FIRMINO, A. A. P.; OLDACH, K.; MURRAY, A. G. W.; CALLAHAM, D. L.; SHEEDY, J.; SILVEIRA, R. E.; CARNEIRO, R. M. D. G.; SA, M. F. G. de; BACIC, A.; ROESSNER, U. A comprehensive approach to identify toxic metabolites present in seeds of leguminous antagonistic plants to juveniles of plant parasitic nematode. In: CONGRESSO BRASILEIRO DE NEMATOLOGIA, 29., 2011, Brasília, DF. Anais... Brasília, DF: Sociedade Brasileira de Nematologia, 2011. p. 257-258.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 144. | | SANTOS, C.; NOGUEIRA, F. C. S.; DOMONT, G. B.; FONTES, W.; PRADO, G. S.; HABIBI, P.; SANTOS, V. O.; OLIVEIRA-NETO, O. B.; SA, M. F. G. de; JORRÍN-NOVO, J. V.; FRANCO, O. L.; REIS, A. M. dos. Corrigendum: proteomic analysis and functional validation of a Brassica oleracea Endochitinase involved in resistance to Xanthomonas campestris. Frontiers in Plant Science, v. 11, article 201, 2020. Na publicação: Maria Fatima Grossi-de-Sá, Angela Mehta.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 145. | | LIMA, L. M. de; VIEIRA, P. M. M. M.; VALENCIA, A. J.; CARNEIRO, R. M. D. G.; MARANHÃO, A. Q.; BRÍGIDO, M. de M.; SÁ, M. F. G. de. Caracterização de scFv selecionado contra proteínas de Meloidogyne incógnita a partir de uma biblioteca de Phage Display. In: CONGRESSO BRASILEIRO DO ALGODÃO, 6., 2007, Uberlândia. Resumos... Uberlândia, 2007. p. 1-5 1 CD-ROM| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Algodão. |
| |
| 146. | | LIMA, L. M. de; VIEIRA, P. M. M. M.; VALENCIA, A. J.; CARNEIRO, R. M. D. G.; MARANHÃO, A. Q.; BRÍGIDO, M. de M.; SÁ, M. F. G. de. Caracterização de scFv selecionado contra proteíncas de Meloidogyne incógnita a partir de uma biblioteca de Phage Display. In: CONGRESSO BRASILEIRO DO ALGODÃO, 6., 2007, Uberlândia. Resumos... Uberlândia, 2007. p. 16| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Algodão. |
| |
| 147. | | ARRAES, F. B. M.; ROCHA, S.; SÁ, D. M. de; FAHEEM, M.; MELO, B. P.; MORGANTE, C. V.; SOUZA JUNIOR, D.; NORIEGA, D.; SA, M. F. G. de; DANCHIN, E. G. In silico analysis reveal high variability in RNAi machinery of five different insect orders. In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
| |
| 148. | | REIS, A. M. dos; MAGALHÃES, B. S.; SOUZA, D. S. L.; VASCONCELOS, E. A. R.; SILVA, L. P. da; SA, M. F. G. de; FRANCO, O. L.; COSTA, P. H. A. da; ROCHA, T. L. Rooteomics: the challenge of discovering plant defense-related proteins in roots. Current Protein Peptide Science, v. 9, p. 108-116, 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 149. | | ARAGÃO, F. J. L.; BRASILEIRO, A. C. M.; BARROS, L. M. G.; ALMEIDA, E. R.; SA, M. F. G. de; GANDER, E. S.; CASTRO, L. A. B. de; RECH, E. L. Transgenic bean (Phaseolus vulgaris L.) plants containing a Brazil nut methionine-rich albumin gene and their progeny derived by microprojectile bombardment of embryonic axes. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR, 23., 1994, Caxambu, MG. Anais. Caxambu: SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR, 1994. p.E-65. p. E-65| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 150. | | SILVA, P. L. R.; MACEDO, L. L. P. de; SÁ, M. E. L. de; AMORIM. R. M. S. de; MORGANTE, C. V.; TESSUTTI, I. T. L.; BASSO, M. F.; GALBIERI, R.; SÁ, M. F. G. de. Transgenic cotton applied to phytonematode control using in plant RNA interfering strategy. In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
| |
| 151. | | NORIEGA, D. D.; ARRAES, F. B. M.; ANTONINO, J. D.; MACEDO, L. L. P. de; FONSECA, F. C. A.; TOGAWA, R. C.; GRYNBERG, P.; SILVA, M. C. M. da; NEGRISOLI JUNIOR, A. S.; SA, M. F. G. de. Transcriptome Analysis and Knockdown of the Juvenile Hormone Esterase Gene Reveal Abnormal Feeding Behavior in the Sugarcane Giant Borer. Frontiers in Physiology, v. 11, 588450, jul. 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
| |
| 152. | | MIRANDA, V. J.; COELHO, R. R.; VIANA, A. A. B.; OLIVEIRA NETO, O. B.; CARNEIRO, R. M. D. G.; ROCHA, T. L.; SA, M. F. G. de; FRAGOSO, R. da R. Validation of reference genes aiming accurate normalization of qPCR data in soybean upon nematode parasitism and insect attack. BMC Research Notes, v. 6, n. 196-205, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 153. | | HABIB, P.; SOCCOL, C. R.; O'KEEFE, B. R.; KRUMPE, L. R. H.; WILSON, J.; MACEDO, L. L. P. de; FAHEEM, M.; SANTOS, V. O. dos; PRADO, G. S.; BOTELHO, M. A.; LACOMBE, S.; SA, M. F. G. de. Gene-silencing suppressors for high-level production of the HIV-1 entry inhibitor griffithsin in Nicotiana benthamiana. Process Biochemistry, v. 70, p. 45-54, 2018. Na publicação: Maria Fatima Grossi-de-Sa.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|  |
| 154. | | GROSSI de SÁ, M.; SOUZA, D. S. L.; FRAGOSO, R. da R.; PINTO, E. R. de C.; ROCHA, T. L.; BARBOSA, A. E. A. D.; SA, M. F. G. de. Functional analysis of genes involved with phytoparasitism of meloidogyne incognita. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programas e resumos... [Brasília, DF]: Sociedade Brasileira de Biotecnologia, 2010. p. 271. p. 271| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
| |
| 155. | | PINHEIRO, M. P. N.; MIRANDA, V. DE J.; BATISTA, V. G. L.; PORTO, M. S.; OLIVEIRA NETO, O. B.; SÁ, M. F. G. DE; MELO FILHO, P. A.; SANTOS, R. C. dos; LIMA, L. M. de. Expressão relativa do gene ovu em vários estádios de desenvolvimento de botão floral de algodoeiro. In: CONGRESSO BRASILEIRO DE MAMONA, 6.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 3., 2014, Fortaleza. Energia e segurança alimentar na agricultura familiar: anais. Campina Grande, PB: Embrapa Algodão, 2014. p. 16| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
| |
| 156. | | GOMES JUNIOR, J. E.; MACEDO, L. L. P.; WERNECK, J. O. S.; SOUZA JUNIOR, J. D. A. de; CRAVEIRO, K. I. C.; SILVA, M. C. M. da; SA, M. F. G. de. Expression of variant CRY1lA12 toxins in Bacillus thuringiensis aiming to control the sugarcane giant borer (Telchin licus licus). In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 234.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 157. | | CRUZ, F.; KALAOUN, S.; NOBILE, P.; COLOMBO, C.; ALMEIDA, J. D. de; BARROS, L. M. G.; PINTO, E. R. de C.; SA, M. F. G. de; VASLIN, M.; ALVES FERREIRA, M. Evaluation of coffee reference genes for relative expression studies by quantitative real-time RT-PCR. Molecular Breeding, v. 23, p. 607-616, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 158. | | FARIAS, D. F.; VIANA, M. P.; OLIVEIRA, G. R. de; BENEVENTI, M. A.; SOARES, B. M.; PESSOA, C.; PESSOA, I. P.; SILVA, L. P.; VASCONCELOS, I. M.; SA, M. F. G. de; CARVALHO, A. F. U. Evaluation of cytotoxic and antimicrobial effects of two Bt Cry proteins on a GMO safety perspective. BioMed Research International, article ID 810490, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 159. | | NARDELI, S. M.; ARGE, L. W. P.; ARTICO, S.; MOURA, S. M. de; TSCHOEKE, D. A.; GUEDES, F. A. de F.; SA, M. F. G. de; MARTINELLI, A. P.; ALVES‑FERREIRA, M. Global gene expression profile and functional analysis reveal the conservation of reproduction‑associated gene networks in Gossypium hirsutum. Plant Reproduction, 2024 On-line first. Na publicação: Maria Fatima Grossi‑de‑Sa.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 160. | | VALENCIA, J. W. A.; JIMÉNEZ, A. V.; SIQUEIRA, F. G. de; MEDINA, K. K.; FRANCO, G. M. R.; FERREIRA FILHO, E. X.; SIEGFRIED, B. D.; SA, M. F. G. de. Holocellulase activity from Schizophyllum commune grown on bamboo: a comparison with different substrates. Current Microbiology, v. 63, p. 581-587, 2011| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| Registros recuperados : 249 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
