| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Semiárido. |
|
Data corrente: |
03/10/2022 |
|
Data da última atualização: |
13/01/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
MOREIRA, V. J. V; LOURENCO, I. T.; BASSO, M. F.; SÁ. M. E. L. de; MORGANTE, C. V.; MELO, B. P.; ARRAES, F. B. M.; SÁ, D. M. de; SILVA, M. C. M. da; ENGLER, J. de A.; SA, M. F. G. de. |
|
Afiliação: |
VALDEIR JUNIO VAZ MOREIRA, UnB; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; MARCOS FERNANDO BASSO, National Institute of Science and Technology - INCT; MARIA EUGÊNIA LISEI DE SA, UnB; CAROLINA VIANNA MORGANTE, CPATSA; BRUNO PAES DE MELO, UFV; FABRÍCIO BARBOSA MONTEIRO ARRAES, National Institute of Science and Technology - INCT; DIOGO MARTINS DE SA, UnB; MARIA CRISTINA MATTAR DA SILVA, Cenargen; JANICE DE ALMEIDA ENGLER, National Institute of Science and Technology - INCT; MARIA FATIMA GROSSI DE SA, Cenargen. |
|
Título: |
Minc03328 effector gene down regulation severely affects Meloidogyne incognita parasitism in transgenic Arabidopsis thaliana. |
|
Ano de publicação: |
2022 |
|
Fonte/Imprenta: |
Planta, v. 255, n. 2, 2022. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Minc03328 effector gene downregulation triggered by in planta RNAi strategy strongly reduced plant susceptibility to Meloidogyne incognita and suggests that Minc03328 gene is a promising target for the development of genetically engineered crops to improve plant tolerance to M. incognita. Meloidogyne incognita is the most economically important species of root-knot nematodes (RKN) and causes severe damage to crops worldwide. M. incognita secretes several effector proteins to suppress the host plant defense response, and manipulate the plant cell cycle and other plant processes facilitating its parasitism. Different secreted effector proteins have already been identified in M. incognita, but not all have been characterized or have had the confirmation of their involvement in nematode parasitism in their host plants. Herein, we characterized the Minc03328 (Minc3s00020g01299) effector gene, confirmed its higher expression in the early stages of M. incognita parasitism in plants, as well as the accumulation of the Minc03328 effector protein in subventral glands and its secretion. We also discuss the potential for simultaneous downregulation of its paralogue Minc3s00083g03984 gene. Using the in planta RNA interference strategy, Arabidopsis thaliana plants overexpressing double-stranded RNA (dsRNA) were generated to specifically targeting and downregulating the Minc03328 gene during nematode parasitism. Transgenic Minc03328-dsRNA lines that significantly downregulated Minc03328 gene expression during M. incognita parasitism were significantly less susceptible. The number of galls, egg masses, and [galls/egg masses] ratio were reduced in these transgenic lines by up to 85%, 90%, and 87%, respectively. Transgenic Minc03328-dsRNA lines showed the presence of fewer and smaller galls, indicating that parasitism was hindered. Overall, data herein strongly suggest that Minc03328 effector protein is important for M. incognita parasitism establishment. As well, the in planta Minc03328-dsRNA strategy demonstrated high biotechnological potential for developing crop species that could efficiently control RKN in the field. MenosMinc03328 effector gene downregulation triggered by in planta RNAi strategy strongly reduced plant susceptibility to Meloidogyne incognita and suggests that Minc03328 gene is a promising target for the development of genetically engineered crops to improve plant tolerance to M. incognita. Meloidogyne incognita is the most economically important species of root-knot nematodes (RKN) and causes severe damage to crops worldwide. M. incognita secretes several effector proteins to suppress the host plant defense response, and manipulate the plant cell cycle and other plant processes facilitating its parasitism. Different secreted effector proteins have already been identified in M. incognita, but not all have been characterized or have had the confirmation of their involvement in nematode parasitism in their host plants. Herein, we characterized the Minc03328 (Minc3s00020g01299) effector gene, confirmed its higher expression in the early stages of M. incognita parasitism in plants, as well as the accumulation of the Minc03328 effector protein in subventral glands and its secretion. We also discuss the potential for simultaneous downregulation of its paralogue Minc3s00083g03984 gene. Using the in planta RNA interference strategy, Arabidopsis thaliana plants overexpressing double-stranded RNA (dsRNA) were generated to specifically targeting and downregulating the Minc03328 gene during nematode parasitism. Transgenic Minc03328-dsRNA lines that significantly downregulated Minc03328 ge... Mostrar Tudo |
|
Palavras-Chave: |
Interação planta-nematóide; Minc03328; Nematoide das galhas; Novas ferramentas biotecnológica; Planta RNAi; Proteção de cultivos; Proteína efetora. |
|
Thesagro: |
Meloidogyne Incognita; Nematóide; Planta. |
|
Thesaurus Nal: |
Plants (botany); Root-knot nematodes. |
|
Categoria do assunto: |
H Saúde e Patologia |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1147069/1/Minc03328-effector-gene-downregulation-2022.pdf
|
|
Marc: |
LEADER 03276naa a2200385 a 4500
001 2147069
005 2023-01-13
008 2022 bl uuuu u00u1 u #d
100 1 $aMOREIRA, V. J. V
245 $aMinc03328 effector gene down regulation severely affects Meloidogyne incognita parasitism in transgenic Arabidopsis thaliana.$h[electronic resource]
260 $c2022
520 $aMinc03328 effector gene downregulation triggered by in planta RNAi strategy strongly reduced plant susceptibility to Meloidogyne incognita and suggests that Minc03328 gene is a promising target for the development of genetically engineered crops to improve plant tolerance to M. incognita. Meloidogyne incognita is the most economically important species of root-knot nematodes (RKN) and causes severe damage to crops worldwide. M. incognita secretes several effector proteins to suppress the host plant defense response, and manipulate the plant cell cycle and other plant processes facilitating its parasitism. Different secreted effector proteins have already been identified in M. incognita, but not all have been characterized or have had the confirmation of their involvement in nematode parasitism in their host plants. Herein, we characterized the Minc03328 (Minc3s00020g01299) effector gene, confirmed its higher expression in the early stages of M. incognita parasitism in plants, as well as the accumulation of the Minc03328 effector protein in subventral glands and its secretion. We also discuss the potential for simultaneous downregulation of its paralogue Minc3s00083g03984 gene. Using the in planta RNA interference strategy, Arabidopsis thaliana plants overexpressing double-stranded RNA (dsRNA) were generated to specifically targeting and downregulating the Minc03328 gene during nematode parasitism. Transgenic Minc03328-dsRNA lines that significantly downregulated Minc03328 gene expression during M. incognita parasitism were significantly less susceptible. The number of galls, egg masses, and [galls/egg masses] ratio were reduced in these transgenic lines by up to 85%, 90%, and 87%, respectively. Transgenic Minc03328-dsRNA lines showed the presence of fewer and smaller galls, indicating that parasitism was hindered. Overall, data herein strongly suggest that Minc03328 effector protein is important for M. incognita parasitism establishment. As well, the in planta Minc03328-dsRNA strategy demonstrated high biotechnological potential for developing crop species that could efficiently control RKN in the field.
650 $aPlants (botany)
650 $aRoot-knot nematodes
650 $aMeloidogyne Incognita
650 $aNematóide
650 $aPlanta
653 $aInteração planta-nematóide
653 $aMinc03328
653 $aNematoide das galhas
653 $aNovas ferramentas biotecnológica
653 $aPlanta RNAi
653 $aProteção de cultivos
653 $aProteína efetora
700 1 $aLOURENCO, I. T.
700 1 $aBASSO, M. F.
700 1 $aSÁ. M. E. L. de
700 1 $aMORGANTE, C. V.
700 1 $aMELO, B. P.
700 1 $aARRAES, F. B. M.
700 1 $aSÁ, D. M. de
700 1 $aSILVA, M. C. M. da
700 1 $aENGLER, J. de A.
700 1 $aSA, M. F. G. de
773 $tPlanta$gv. 255, n. 2, 2022.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Semiárido (CPATSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 91 | |
| 9. |  | SA, M. E. L. de; SILVA, H. E. da; SILVA, C. S.; SANTOS, C. B. Indução de embriogênese somática em macaúba. In: CONGRESSO DA REDE BRASILEIRA DE TECNOLOGIA DE BIODIESEL, 3., 2009, Brasília, DF. Anais... Brasília, DF: MCT: MBC, 2009. p. 527-528.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
|   |
| 10. | | SÁ, M. E. L. de; CARRÃO-PANIZZI, M. C.; ARANTES, N. E.; ZITO, R. K. Influência da época e da população de plantas no rendimento de soja hortaliça. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 30., 2008, Rio Verde. Resumos... Londrina: Embrapa Soja, 2008. p. 59-61. (Embrapa Soja. Documentos, 304). Organizado por Odilon Ferreira Saraiva, César de Castro, Regina Maria Villas Bôas de Campos Leite, Fábio Alvares de Oliveira.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
| |
| 11. | | ARF, O.; RODRIGUES, R. A. F.; SÁ, M. E. de; CRUSCIOL, C. A. C. Influência da época de semeadura no comportamento de cultivares de arroz irrigado por aspersão em Selviria, MS. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 35, n. 10, p. 1967-76, out. 2000. Título em inglês: Effects of sowing dates on performance of dryland rice cultivars under sprinkler irrigation, in Selvíria, MS, Brazil.| Biblioteca(s): Embrapa Unidades Centrais. |
|  |
| 12. | | SÁ, M. E. L. de; ZITO, R. K.; MORAIS, F. C.; ARANTES, N. E. Fungicide effect in soybean sensorial characteristics in two environments. In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. p. 287. (Embrapa Soja. Documentos, 228). Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.| Biblioteca(s): Embrapa Soja. |
| |
| 16. | | CRUSCIOL, C. A. C.; LAZARINI, E.; GOLFETO, A. R.; SÁ, M. E. de. Produtividade e componentes da produção do amendoim da seca em razão da época de semeadura e da aplicação de cálcio. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 35, n. 8, p. 1549-1558, ago. 2000. Título em inglês: Productiveness and yield components in dry season peanut as affected by planting time and calcium fertilization.| Biblioteca(s): Embrapa Unidades Centrais. |
| |
| 17. | | ARF, O.; RODRIGUES, R. A. F.; SÁ, M. E. de; CRUSCIOL, C. A. C. Resposta de cultivares de arroz de sequeiro ao preparo do solo e a irrigação por aspersão. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 36, n. 6, p. 871-79, jun. 2001 Título em inglês: Performance of dryland rice cultivars in function of soil preparation systems and sprinkler irrigation.| Biblioteca(s): Embrapa Unidades Centrais. |
| |
| 19. | | ARF, O.; RODRIGUES, R. A. F.; SÁ, M. E. de; BUZETTI, S.; NASCIMENTO, V. do. Manejo do solo, água e nitrogênio no cultivo de feijão. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 39, n. 2, p. 131-138, fev. 2004 Título em inglês: Soil, water and nitrogen management in common bean cultivation.| Biblioteca(s): Embrapa Unidades Centrais. |
| |
| 20. | | MEIRA, F. de A.; SÁ, M. E. de; BUZETTI, S.; ARF, O. Doses e épocas de aplicação de nitrogênio no feijoeiro irrigado cultivado em plantio direto. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 40, n. 4, p. 383-388, abr. 2005 Título em inglês: Doses and time of nitrogen top-dressed application for common bean in no-till system.| Biblioteca(s): Embrapa Unidades Centrais. |
| |
| Registros recuperados : 91 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
