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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte; Embrapa Gado de Leite. |
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Data corrente: |
17/03/2014 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAUJO, C. P.; OSÓRIO, A. L. A. R.; JORGE, K. S. G.; RAMOS, C. A. N.; S. FILHO, A. F.; VIDAL, C. E. S.; ROXO, E.; NISHIBE, c.; ALMEIDA, N. F.; F. JUNIOR, A. A.; SILVA, M. R.; B. NETO, J. D.; CERQUEIRA, V. D.; ZUMÁRRAGA, M.; ARAUJO, F. R. |
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Afiliação: |
MARCIO ROBERTO SILVA, CNPGL. |
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Título: |
Detection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plos One, v. 9, n. 3, p. 1-6, 2014 |
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Idioma: |
Inglês
Português |
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Conteúdo: |
In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with NVL. However, nested-PCR detected a significantly higher number of positive animals than the culture in the group of animals exhibiting LCT with no previous records of CITT. The use of the nested-PCR assay to detect M. bovis in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis. MenosIn the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with ... Mostrar Tudo |
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Palavras-Chave: |
Bovine and bubaline tuberculosis; Nested-PCR system. |
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Thesaurus Nal: |
Mycobacterium abscessus. |
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Categoria do assunto: |
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L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/119848/1/ARAUJO-C.-P..pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/99300/1/pone.0091023-1..6-2014-Nested-PCR.pdf
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Marc: |
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100 1 $aARAUJO, C. P.
245 $aDetection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1.$h[electronic resource]
260 $c2014
520 $aIn the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with NVL. However, nested-PCR detected a significantly higher number of positive animals than the culture in the group of animals exhibiting LCT with no previous records of CITT. The use of the nested-PCR assay to detect M. bovis in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis.
650 $aMycobacterium abscessus
653 $aBovine and bubaline tuberculosis
653 $aNested-PCR system
700 1 $aOSÓRIO, A. L. A. R.
700 1 $aJORGE, K. S. G.
700 1 $aRAMOS, C. A. N.
700 1 $aS. FILHO, A. F.
700 1 $aVIDAL, C. E. S.
700 1 $aROXO, E.
700 1 $aNISHIBE, c.
700 1 $aALMEIDA, N. F.
700 1 $aF. JUNIOR, A. A.
700 1 $aSILVA, M. R.
700 1 $aB. NETO, J. D.
700 1 $aCERQUEIRA, V. D.
700 1 $aZUMÁRRAGA, M.
700 1 $aARAUJO, F. R.
773 $tPlos One$gv. 9, n. 3, p. 1-6, 2014
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 6 | |
| 1. |  | MORETTI, C. L.; ARAUJO, A. L.; SILVA, W. L. C.; KRAMMES, J. G.; FERREIRA, K. S.; SILVA, V. J. M.; NUNES, F. P.; S. FILHO, A. R. da. Avaliacao da qualidade de pimentao minimamente processado. Horticultura Brasilieira, Brasilia, v.18, p.329-330, jul. 2000. Suplemento. Trabalho apresentado no 40. Congresso Brasileiro de Olericultura, 2000.| Biblioteca(s): Embrapa Hortaliças. |
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| 2. | | CARVALHO, E. J. M.; FREITAS, L. de S.; S. FILHO, A.; VELOSO, C. A. C.; VALENTE, M. A.; EL-HUSNI, J. C. Influência de sistemas de manejo sobre a matéria orgânica em solo de cerrado no Sudeste paraense. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 30., 2005, Recife. Solos, sustentabilidade e qualidade ambiental. Recife: Sociedade Brasileira de Ciência do Solo, 2005.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 3. | | GAMA, J. R. N. F.; CARVALHO, E. J. M.; FREITAS, L. de S.; S. FILHO, A.; VALENTE, M. A.; EL-HUSNY, J. C. Efeito de sistemas de manejo sobre alumínio trocável e pH do solo em área de cerrado no Sudeste paraense. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 30., 2005, Recife. Solos, sustentabilidade e qualidade ambiental. Recife: Sociedade Brasileira de Ciência do Solo, 2005.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 4. | | FARIA, D. A. de; PALLAORO, D. S.; ZUCHINI, L. A. da S.; CAMPOS, D. T. da S.; FILHO, A. B. B. Teor de carbobo microbiano em solos de cerrados sob cultivo de girassol. In: REUNIÃO NACIONAL DE PESQUISA DO GIRASSOL, 18., SIMPÓSIO NACIONAL SOBRE A CULTURA DO GIRASSOL, 6., 2009. Pelotas. Anais... Pelotas: Embrapa Clima Temperado, 2009. p. 248-252. Editado por Ana Claudia Barneche de Oliveira, Ana Paula Afonso Scheneid da Rosa e Márcia Vizzotto.| Biblioteca(s): Embrapa Clima Temperado. |
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| 5. | | OLIVEIRA, M. S. de; OLIVEIRA, H. A. de; S. FILHO, A. P. S.; ISHIDA, A. K. N.; VASCONCELOS, M. A. M. de; VENTURIERI, G. C.; PACHECO, L. C.; AZEVEDO, T. L. de. Avaliação do efeito de substâncias presentes em frações de geoprópolis da abelha sem ferrão Scaptotrigona sp., obtidas em coluna aberta sobre o crescimento de Xanthomonas axonopodis pv. passiflorae (Pereira) Dye.). In: CONGRESSO BRASILEIRO DE QUÍMICA, 53., 2013, Natal. Química e sociedade: motores da sustentabilidade: anais. Natal: ABQ; UFRN, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 6. | | ARAUJO, C. P.; OSÓRIO, A. L. A. R.; JORGE, K. S. G.; RAMOS, C. A. N.; S. FILHO, A. F.; VIDAL, C. E. S.; ROXO, E.; NISHIBE, c.; ALMEIDA, N. F.; F. JUNIOR, A. A.; SILVA, M. R.; B. NETO, J. D.; CERQUEIRA, V. D.; ZUMÁRRAGA, M.; ARAUJO, F. R. Detection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1. Plos One, v. 9, n. 3, p. 1-6, 2014| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Corte; Embrapa Gado de Leite. |
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| Registros recuperados : 6 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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