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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
11/09/2023 |
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Data da última atualização: |
11/09/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DIONISIO, J. F.; PEZENTI, L. F.; SOUZA, R. F. de; SÓSA-GOMEZ, D. R.; ROSA, R. da. |
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Afiliação: |
JAQUELINE FERNANDA DIONISIO, UEL; LARISSA FORIM PEZENTI, UEL; ROGERIO FERNANDES DE SOUZA, UEL; DANIEL RICARDO SOSA GOMEZ, CNPSO; RENATA DA ROSA, UEL. |
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Título: |
Annotation of transposable elements in the transcriptome of the Neotropical brown stink bug Euschistus heros and its chromosomal distribution. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Molecular Genetics and Genomics, 2023. |
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Páginas: |
12 p. |
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DOI: |
10.1007/s00438-023-02063-9 |
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Idioma: |
Inglês |
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Notas: |
first on line. |
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Thesagro: |
Entomologia; Hibridação; Inseto. |
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Thesaurus Nal: |
Entomology; Fluorescence in situ hybridization; Insects. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 00821naa a2200265 a 4500
001 2156558
005 2023-09-11
008 2023 bl uuuu u00u1 u #d
024 7 $a10.1007/s00438-023-02063-9$2DOI
100 1 $aDIONISIO, J. F.
245 $aAnnotation of transposable elements in the transcriptome of the Neotropical brown stink bug Euschistus heros and its chromosomal distribution.$h[electronic resource]
260 $c2023
300 $a12 p.
500 $afirst on line.
650 $aEntomology
650 $aFluorescence in situ hybridization
650 $aInsects
650 $aEntomologia
650 $aHibridação
650 $aInseto
700 1 $aPEZENTI, L. F.
700 1 $aSOUZA, R. F. de
700 1 $aSÓSA-GOMEZ, D. R.
700 1 $aROSA, R. da
773 $tMolecular Genetics and Genomics, 2023.
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Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
10/01/2005 |
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Data da última atualização: |
03/08/2017 |
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Autoria: |
GRANGE, L.; HUNGRIA, M. |
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Título: |
Genetic diversity of indigenous common bean (Phaseolus vulgaris) rhizobia in two Brazilian ecosystems. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Soil Biology and Biochemistry, Oxford, v. 36, n. 9, p. 1389-1398, Sept. 2004. |
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Idioma: |
Inglês |
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Conteúdo: |
Although rhizobia for common bean (Phaseolus vulgaris L.) are established in most Brazilian soils, understanding of their genetic diversity is very poor. This study characterized bean strains from two contrasting ecosystems in Brazil, the Northeast Region, with a semi-arid climate and neutral soils and the South Region, with a humid subtropical climate and acid soils. Seedlings of the cultivars Negro Argel and Aporé were used to trap 243 rhizobial isolates from 12 out of 14 sites. An analysis of ERIC-PCR products revealed enormous variability, with 81% of the isolates representing unique strains considering a level of 70% of similarity. In general, there was no effect of either the bean cultivar, or the ecosystem on rhizobial diversity. One-hundred and one strains showing genetic relatedness (ERIC-PCR) less than 70% were further analyzed using restriction fragment length polymorphism (RFLP) of the 16 S rDNA cleaved with five restriction enzymes. Twenty-five different profile combinations were obtained. Rhizobium etli was the predominant species, with 73 strains showing similar RFLP profiles, while 12 other strains differed only by the profile with one restriction enzyme. Fifty strains were submitted to sequencing of a 16 S rDNA fragment, and 34 clustered with R. etli, including strains with RFLP-PCR profiles similar to those species or differing by one restriction enzyme. However, other strains differing by one or two enzymes were genetically distant from R. etli and two strains with identical profiles showed higher similarity to Sinorhizobium fredii. Other strains showed higher similarity of bases with R. tropici, R. leguminosarum and Mesorhizobium plurifarium, but some strains were quite dissimilar and may represent new species. Great variability was also verified among the sequenced strains in relation to the ability to grow in YMA at 40 °C, in LB, to synthesize melanin in vitro, as well as in symbiotic performance, including differences in relation to the described species, e.g. many R. etli strains were able to grow in LB and in YMA at 40 °C, and not all R. tropici were able to nodulate Leucaena. MenosAlthough rhizobia for common bean (Phaseolus vulgaris L.) are established in most Brazilian soils, understanding of their genetic diversity is very poor. This study characterized bean strains from two contrasting ecosystems in Brazil, the Northeast Region, with a semi-arid climate and neutral soils and the South Region, with a humid subtropical climate and acid soils. Seedlings of the cultivars Negro Argel and Aporé were used to trap 243 rhizobial isolates from 12 out of 14 sites. An analysis of ERIC-PCR products revealed enormous variability, with 81% of the isolates representing unique strains considering a level of 70% of similarity. In general, there was no effect of either the bean cultivar, or the ecosystem on rhizobial diversity. One-hundred and one strains showing genetic relatedness (ERIC-PCR) less than 70% were further analyzed using restriction fragment length polymorphism (RFLP) of the 16 S rDNA cleaved with five restriction enzymes. Twenty-five different profile combinations were obtained. Rhizobium etli was the predominant species, with 73 strains showing similar RFLP profiles, while 12 other strains differed only by the profile with one restriction enzyme. Fifty strains were submitted to sequencing of a 16 S rDNA fragment, and 34 clustered with R. etli, including strains with RFLP-PCR profiles similar to those species or differing by one restriction enzyme. However, other strains differing by one or two enzymes were genetically distant from R. etli and two str... Mostrar Tudo |
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Palavras-Chave: |
Rizóbio. |
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Thesagro: |
Feijão. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02618naa a2200157 a 4500
001 1467760
005 2017-08-03
008 2004 bl --- 0-- u #d
100 1 $aGRANGE, L.
245 $aGenetic diversity of indigenous common bean (Phaseolus vulgaris) rhizobia in two Brazilian ecosystems.
260 $c2004
520 $aAlthough rhizobia for common bean (Phaseolus vulgaris L.) are established in most Brazilian soils, understanding of their genetic diversity is very poor. This study characterized bean strains from two contrasting ecosystems in Brazil, the Northeast Region, with a semi-arid climate and neutral soils and the South Region, with a humid subtropical climate and acid soils. Seedlings of the cultivars Negro Argel and Aporé were used to trap 243 rhizobial isolates from 12 out of 14 sites. An analysis of ERIC-PCR products revealed enormous variability, with 81% of the isolates representing unique strains considering a level of 70% of similarity. In general, there was no effect of either the bean cultivar, or the ecosystem on rhizobial diversity. One-hundred and one strains showing genetic relatedness (ERIC-PCR) less than 70% were further analyzed using restriction fragment length polymorphism (RFLP) of the 16 S rDNA cleaved with five restriction enzymes. Twenty-five different profile combinations were obtained. Rhizobium etli was the predominant species, with 73 strains showing similar RFLP profiles, while 12 other strains differed only by the profile with one restriction enzyme. Fifty strains were submitted to sequencing of a 16 S rDNA fragment, and 34 clustered with R. etli, including strains with RFLP-PCR profiles similar to those species or differing by one restriction enzyme. However, other strains differing by one or two enzymes were genetically distant from R. etli and two strains with identical profiles showed higher similarity to Sinorhizobium fredii. Other strains showed higher similarity of bases with R. tropici, R. leguminosarum and Mesorhizobium plurifarium, but some strains were quite dissimilar and may represent new species. Great variability was also verified among the sequenced strains in relation to the ability to grow in YMA at 40 °C, in LB, to synthesize melanin in vitro, as well as in symbiotic performance, including differences in relation to the described species, e.g. many R. etli strains were able to grow in LB and in YMA at 40 °C, and not all R. tropici were able to nodulate Leucaena.
650 $aFeijão
653 $aRizóbio
700 1 $aHUNGRIA, M.
773 $tSoil Biology and Biochemistry, Oxford$gv. 36, n. 9, p. 1389-1398, Sept. 2004.
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