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Registro Completo |
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Biblioteca(s): |
Embrapa Café; Embrapa Unidades Centrais. |
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Data corrente: |
18/02/2011 |
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Data da última atualização: |
07/07/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RAMIRO, D.; JALLOUL, A.; PETITOT, A-S.; SA, M. F. G. de; MALUF, M. P.; FERNANDEZ, D. |
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Afiliação: |
DANIEL RAMIRO, CIRAD; AIDA JALLOUL, University of Damascus; ANNE-SOPHIE PETITOT, CIRAD; MARIA FATIMA GROSSI DE SA, CENARGEN; MIRIAN PEREZ MALUF, SAPC; DIANA FERNANDEZ, CIRAD. |
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Título: |
Identification of coffee WRKY transcription factor genes and expression in resistance responses to pathogens. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
TREE GENETICS & GENOMES, Curitiba, v.6, p.767-781. 2010. |
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Páginas: |
767-781 |
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Idioma: |
Português |
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Conteúdo: |
In plants, WRKY proteins are a group of transcription factors existing as a gene superfamily that play important roles in regulation of defense response pathways. To assess the diversity of this protein family in coffee (Coffea spp.), data mining methods were used on a set of around 200,000 coffee expressed sequence tags. A total of 53 different putative WRKY genes were obtained, but only 22 unigenes encoding a protein with a WRKY domain were identified, eight of which are supported by full-length cDNA sequences. Alignment of WRKY domain sequences of the coffee unigenes and 72 Arabidopsis thaliana WRKY genes showed that the 22 coffee WRKY members were distributed among the main A. thaliana WRKY subgroups and shared conserved peptide domains. To assess the involvement of WRKY genes in coffee defense response pathways, their expression was analyzed under biotic (nematode and rust fungus infection), hormonal (salicylic acid, methyl-jasmonate), and wounding treatments, leaf senescence, and fruit development. Five members of WRKY groups IId and III were regulated only by pathogens and hormone treatments. Although a significant correlation of WRKY genes expression after MeJA and rust treatments was observed, expression of coffee genes involved in JA biosynthesis and lipoxygenase (EC 1.13.11.12) activity assays did not support the involvement of JA in the early coffee resistance responses to the rust pathogen. The five WRKY transcription factor members identified might play important roles as regulators of pathogen resistance responses and could be useful for improving coffee tolerance to various biotic stresses. MenosIn plants, WRKY proteins are a group of transcription factors existing as a gene superfamily that play important roles in regulation of defense response pathways. To assess the diversity of this protein family in coffee (Coffea spp.), data mining methods were used on a set of around 200,000 coffee expressed sequence tags. A total of 53 different putative WRKY genes were obtained, but only 22 unigenes encoding a protein with a WRKY domain were identified, eight of which are supported by full-length cDNA sequences. Alignment of WRKY domain sequences of the coffee unigenes and 72 Arabidopsis thaliana WRKY genes showed that the 22 coffee WRKY members were distributed among the main A. thaliana WRKY subgroups and shared conserved peptide domains. To assess the involvement of WRKY genes in coffee defense response pathways, their expression was analyzed under biotic (nematode and rust fungus infection), hormonal (salicylic acid, methyl-jasmonate), and wounding treatments, leaf senescence, and fruit development. Five members of WRKY groups IId and III were regulated only by pathogens and hormone treatments. Although a significant correlation of WRKY genes expression after MeJA and rust treatments was observed, expression of coffee genes involved in JA biosynthesis and lipoxygenase (EC 1.13.11.12) activity assays did not support the involvement of JA in the early coffee resistance responses to the rust pathogen. The five WRKY transcription factor members identified might play importa... Mostrar Tudo |
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Palavras-Chave: |
Rust disease. |
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Thesagro: |
Coffea Arábica. |
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Thesaurus Nal: |
Regulator genes. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/29339/1/Identification-of-coffee.pdf
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Marc: |
LEADER 02308naa a2200229 a 4500
001 1880488
005 2011-07-07
008 2010 bl uuuu u00u1 u #d
100 1 $aRAMIRO, D.
245 $aIdentification of coffee WRKY transcription factor genes and expression in resistance responses to pathogens.$h[electronic resource]
260 $c2010
300 $a767-781
520 $aIn plants, WRKY proteins are a group of transcription factors existing as a gene superfamily that play important roles in regulation of defense response pathways. To assess the diversity of this protein family in coffee (Coffea spp.), data mining methods were used on a set of around 200,000 coffee expressed sequence tags. A total of 53 different putative WRKY genes were obtained, but only 22 unigenes encoding a protein with a WRKY domain were identified, eight of which are supported by full-length cDNA sequences. Alignment of WRKY domain sequences of the coffee unigenes and 72 Arabidopsis thaliana WRKY genes showed that the 22 coffee WRKY members were distributed among the main A. thaliana WRKY subgroups and shared conserved peptide domains. To assess the involvement of WRKY genes in coffee defense response pathways, their expression was analyzed under biotic (nematode and rust fungus infection), hormonal (salicylic acid, methyl-jasmonate), and wounding treatments, leaf senescence, and fruit development. Five members of WRKY groups IId and III were regulated only by pathogens and hormone treatments. Although a significant correlation of WRKY genes expression after MeJA and rust treatments was observed, expression of coffee genes involved in JA biosynthesis and lipoxygenase (EC 1.13.11.12) activity assays did not support the involvement of JA in the early coffee resistance responses to the rust pathogen. The five WRKY transcription factor members identified might play important roles as regulators of pathogen resistance responses and could be useful for improving coffee tolerance to various biotic stresses.
650 $aRegulator genes
650 $aCoffea Arábica
653 $aRust disease
700 1 $aJALLOUL, A.
700 1 $aPETITOT, A-S.
700 1 $aSA, M. F. G. de
700 1 $aMALUF, M. P.
700 1 $aFERNANDEZ, D.
773 $tTREE GENETICS & GENOMES, Curitiba$gv.6, p.767-781. 2010.
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Registro original: |
Embrapa Café (CNPCa) |
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| Registros recuperados : 249 | |
| 42. |  | SILVA, C. P.; TERRA, W. R.; XAVIER FILHO, J.; SA, M. F. G. de; BIFANO, T. D. Induction of x-amylases in larvae of the bruchid beetle zabrotes subfasciatus in response to different hosts. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 28., 1999, Caxambu, MG. Programa e resumos. Caxambu: Sociedade Brasileira de Bioquimica e Biologia Molecular, 1999.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 43. | | FREITAS, A. L. A. de; ARGE, L. W. P.; NARDELI, S. M.; SA, M. F. G. de; ALVES-FERREIRA, M. Lutando contra a Herbivoria: respostas transcricionais de algodão (Gossypium hirsutum) sob infestação pelo bicudo do algodoeiro (Anthonomus grandis). In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 8, 2023, Florianópolis, SC. Anais... Florianópolis: SBG, 2023. p. 95 Na publicação: Maria Fátima Grossi-de-sá.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 44. | | MORGANTE, C. V.; ARRAES, F. B. M.; MOREIRA-PINTO, C. E; MELO, B. P.; SA, M. F. G. de. Modulation of gene expression in plants via CRISPR/dCas9 technology. In: MOLINARI, H. B. C.; VIEIRA, L. R.; SILVA, N. V. e; PRADO, G. S.; LOPES FILHO, J. F. (Ed.). CRISPR technology in plant genome editing: biotechnology applied to agriculture. Brasília, DF : Embrapa, 2021. CAP. 4, 119-167 il., color.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| 47. | | SOUZA, D. dos S. de L.; BARBOSA, A. E. A. de D.; SA, M. F. G. de. Estrátegias moleculares para o controle de fitonematóides do algodoeiro. Tropical Plant Pathology, Brasília, DF, v. 34, p. XXXVII, 2009. Suplemento. Edição dos Resumos do XLII do Congresso Brasileiro de Fitopatologia, Rio de Janeiro, RJ, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 49. | | VASCONCELOS, E. A. R.; SILVA, T. S.; MAGALHÃES, J. C. C.; ROCHA, T. L.; SA, M. F. G. de. Proteomic characterization of Bacillus thuringiensis S811 strain and evaluation of proteins toxicity towards Anthonomus grandis. In: ANNUAL MEETING OF THE SBBq, 36.; IUBMB CONFERENCE, 10., 2007, Salvador, BA. Infectious diseases: biochemistry of parasites, vectors and hosts. [S.l.]: SBBq, 2007. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 50. | | SOUZA, C. R.; ALMEIDA, E. R. de; SAMPAIO, M. J.; PEREIRA, P. A. A.; SA, M. F. G. de. Purificação, clonagem e expressão da arcelina em espécies de feijão susceptíveis ao ataque de carunchos: estudo comparativo da expressão obtida através de técnicas de retrocruzamento e engenharia genética. In: REUNIÃO BRASILEIRA SOBRE CONTROLE BIOLÓGICO DE DOENÇAS DE PLANTAS, 3., 1989, Piracicaba. Anais. Piracicaba: ESALQ, 1989. p. 97-98.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 52. | | VIANA, A. A. B.; BARRETO, C. C.; BARROS, L. M. G.; SA, M. F. G. de; CARNEIRO, M. Spatial and temporal verification of the expression pattern of the 2.5 and 1.38(4) promoters in transgenic plants. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 28., 1999, Caxambu, MG. Programa e resumos. Caxambu: Sociedade Brasileira de Bioquimica e Biologia Molecular, 1999. p.38 E-84 Producao cientifica/99| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 53. | | VIANA, A. A. B.; BARRETO, C. C.; BARROS, L. M. G.; SA, M. F. G. de; CARNEIRO, M. Spatial and temporal verification of the expression pattern of the 2.5 and 1.38(4) promoters in transgenic plants. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 28., 1999, Caxambu, MG. Programa e resumos. Caxambu: Sociedade Brasileira de Bioquimica e Biologia Molecular, 1999. p.38-E-84| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 55. | | FRANCO, O. L.; SA, M. F. G. de; SALES, M. P.; MELLO, L. V.; OLIVEIRA, A. S.; RIGDEN, D. J. Overlapping binding sites for trypsin and papain on a kunitz-type proteinase inhibitor from Prosopis juliflora. Protein: Structure, Function, and Genetics, v. 49, p. 335-341, 2002.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 56. | | SILVA, S. G.; PAES, N. S.; BARBOSA, A. E. A. D.; BRASILEIRO, A. C. M.; SA, M. F. G. de. Validação da expressão diferencial de genes envolvidos na resistência de algodão a Meloidogyne incognita POR RT-qPCR. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 15., 2010, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. Resumo 037.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 58. | | SILVA, S. G. da; PAES, N. S.; SA, M. F. G. de; BRASILEIRO, A. C. M.; REIS, A. M. dos. Validation of the differential expression of genes involved in cotton resistance to Meloidogyne Incógnita by RT-QPCR. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 263.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 59. | | SOUZA, D. dos S. de L.; PINTO, E. R. de C.; MOLINARI, H. B. C.; SA, M. F. G. de. Transformação genética de cana-de-açúcar. In: FIGUEIREDO, M. do V. B.; BURITY, H. A.; OLIVEIRA, J. de P.; SANTOS, C. E. de R. e S.; STAMFORD, N. P. (Ed.). Biotecnologia aplicada à agricultura: textos de apoio e protocolos experimentais. Brasília, DF: Embrapa Informação Tecnológica; Recife: Instituto Agronômico de Pernambuco, 2010. p. 333-356| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia. |
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| 60. | | SOUZA, D. dos S. de L.; PINTO, E. R. de C.; MOLINARI, H. B. C.; SA, M. F. G. de. Transformação genética de cana-de-açúcar. In: FIGUEIREDO, M. do V. B.; BURITY, H. A.; OLIVEIRA, J. de P.; SANTOS, C. E. de R. e S.; STAMFORD, N. P. (Ed.). Biotecnologia aplicada à agricultura: textos de apoio e protocolos experimentais. Brasília, DF: Embrapa Informação Tecnológica; Recife: Instituto Agronômico de Pernambuco, 2010. p. 333-356.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 249 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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