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Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Agroindústria Tropical; Embrapa Amazônia Ocidental; Embrapa Arroz e Feijão; Embrapa Cerrados; Embrapa Hortaliças; Embrapa Mandioca e Fruticultura; Embrapa Meio-Norte; Embrapa Pantanal; Embrapa Roraima; Embrapa Tabuleiros Costeiros; Embrapa Uva e Vinho. MenosEmbrapa Agrobiologia; Embrapa Agroindústria Tropical; Embrapa Amazônia Ocidental; Embrapa Arroz e Feijão; Embrapa Cerrados; Embrapa Hortaliças; Embrapa Mandioca e Fruticultura; Embrapa Meio-Norte; Embrapa Pantanal; Embrapa Roraima... Mostrar Todas |
Data corrente: |
21/02/2001 |
Data da última atualização: |
06/07/2010 |
Autoria: |
MELO, F. R.; FRANCO, O. L.; SILVA, M. C. M. da; SÁ, M. F. G. de. |
Título: |
Inibidores protéicos de plantas e suas atividades contra insetos. |
Ano de publicação: |
1999 |
Fonte/Imprenta: |
Brasília: Embrapa Recursos Genéticos e Biotecnologia, 1999. |
Páginas: |
50 p. |
Série: |
(Embrapa Recursos Genéticos e Biotecnologia. Circular técnica, 2). |
Idioma: |
Português |
Conteúdo: |
As pragas e os patogenos (fungos, bacterias e virus) sao responsaveis por grandes perdas da agricultura, causando injurias e doencas, alem de se alimentarem dos tecidos de plantas. As perdas na producao da agricultura mundial, devido ao ataque de pragas e doencas chegam a 37%, sendo que 13% desta perda e causada por insetos (Gatehouse et al., 1992). As plantas, possuem entretanto, um certo grau de resistencia a insetos e ha muitos anos, tem-se estudado a biossintese e a regulacao de compostos quimicos de plantas associadas com essas defesas. Atualmente, sabe-se que esses defensivos sao encontrados em varios tecidos vegetais e entre esses compostos, estao incluidos antibioticos, alcaloides, terpenos e proteinas. Entre as proteinas, estao incluidas enzimas tais como as quitinases, as lectinas e os inibidores de enzimas digestivas (Ryan, 1990). Atualmente, genes que conferem resistencia a insetos podem ser introduzidos em plantas de interesse para reduzir sua susceptibilidade. Esses genes podem ser obtidos de plantas, bacterias ou de outra origem (Schuler et al., 1998). Os inibidores de enzimas (a- amilases e e de proteinases) serao aqui descritos e estudados, relacionando-se suas funcoes como compostos de defesas de plantas contra insetos e seu potencial como ferramenta na obtencao de plantas resistentes a pragas. |
Palavras-Chave: |
Brasil; Brasilia; Genetic control; Inibidor; Insect; Pest insects; Pests of plants; Plant; Plant diseases; Plants; Protein inhibitor; Resistance. |
Thesagro: |
Controle Biológico; Controle Genético; Doença de Planta; Inibidor de Enzima; Inibidor de proteína; Inseto; Planta; Planta Transgênica; Praga; Praga de planta; Proteína; Resistência. |
Thesaurus Nal: |
biological control; enzyme inhibitors; Insecta; transgenic plants. |
Categoria do assunto: |
-- F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 02687nam a2200505 a 4500 001 1208796 005 2010-07-06 008 1999 bl uuuu u0uu1 u #d 100 1 $aMELO, F. R. 245 $aInibidores protéicos de plantas e suas atividades contra insetos. 260 $aBrasília: Embrapa Recursos Genéticos e Biotecnologia$c1999 300 $a50 p. 490 $a(Embrapa Recursos Genéticos e Biotecnologia. Circular técnica, 2). 520 $aAs pragas e os patogenos (fungos, bacterias e virus) sao responsaveis por grandes perdas da agricultura, causando injurias e doencas, alem de se alimentarem dos tecidos de plantas. As perdas na producao da agricultura mundial, devido ao ataque de pragas e doencas chegam a 37%, sendo que 13% desta perda e causada por insetos (Gatehouse et al., 1992). As plantas, possuem entretanto, um certo grau de resistencia a insetos e ha muitos anos, tem-se estudado a biossintese e a regulacao de compostos quimicos de plantas associadas com essas defesas. Atualmente, sabe-se que esses defensivos sao encontrados em varios tecidos vegetais e entre esses compostos, estao incluidos antibioticos, alcaloides, terpenos e proteinas. Entre as proteinas, estao incluidas enzimas tais como as quitinases, as lectinas e os inibidores de enzimas digestivas (Ryan, 1990). Atualmente, genes que conferem resistencia a insetos podem ser introduzidos em plantas de interesse para reduzir sua susceptibilidade. Esses genes podem ser obtidos de plantas, bacterias ou de outra origem (Schuler et al., 1998). Os inibidores de enzimas (a- amilases e e de proteinases) serao aqui descritos e estudados, relacionando-se suas funcoes como compostos de defesas de plantas contra insetos e seu potencial como ferramenta na obtencao de plantas resistentes a pragas. 650 $abiological control 650 $aenzyme inhibitors 650 $aInsecta 650 $atransgenic plants 650 $aControle Biológico 650 $aControle Genético 650 $aDoença de Planta 650 $aInibidor de Enzima 650 $aInibidor de proteína 650 $aInseto 650 $aPlanta 650 $aPlanta Transgênica 650 $aPraga 650 $aPraga de planta 650 $aProteína 650 $aResistência 653 $aBrasil 653 $aBrasilia 653 $aGenetic control 653 $aInibidor 653 $aInsect 653 $aPest insects 653 $aPests of plants 653 $aPlant 653 $aPlant diseases 653 $aPlants 653 $aProtein inhibitor 653 $aResistance 700 1 $aFRANCO, O. L. 700 1 $aSILVA, M. C. M. da 700 1 $aSÁ, M. F. G. de
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca(s): |
Embrapa Solos. |
Data corrente: |
16/11/2018 |
Data da última atualização: |
11/11/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
ALESSI, A. M.; BIRD, S. M.; OATES, N. C.; LI, Y.; DOWLE, A. A.; NOVOTNY, E. H.; AZEVEDO, E. R. de; BENNETT, J. P.; POLIKARPOV, I.; YOUNG, J. P. W.; MCQUEEN-MASON, S. J.; BRUCE, N. C. |
Afiliação: |
ANNA M. ALESSI, UNIVERSITY OF YORK; SUSANNAH M. BIRD, UNIVERSITY OF YORK; NICOLA C. OATES, UNIVERSITY OF YORK; YI LI, UNIVERSITY OF YORK; ADAM A. DOWLE, UNIVERSITY OF YORK; ETELVINO HENRIQUE NOVOTNY, CNPS; EDUARDO R. DE AZEVEDO, USP; JOSEPH P. BENNETT, UNIVERSITY OF YORK; IGOR POLIKARPOV, USP; J. PETER W. YOUNG, UNIVERSITY OF YORK; SIMON J. MCQUEEN-MASON, UNIVERSITY OF YORK; NEIL C. BRUCE, UNIVERSITY OF YORK. |
Título: |
Defining functional diversity for lignocellulose degradation in a microbial community using multi-omics studies. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Biotechnology for Biofuels, v. 11, article 166, 2018. |
DOI: |
https://doi.org/10.1186/s13068-018-1164-2 |
Idioma: |
Inglês |
Conteúdo: |
Background: Lignocellulose is one of the most abundant forms of fixed carbon in the biosphere. Current industrial approaches to the degradation of lignocellulose employ enzyme mixtures, usually from a single fungal species, which are only effective in hydrolyzing polysaccharides following biomass pre-treatments. While the enzymatic mechanisms of lignocellulose degradation have been characterized in detail in individual microbial species, the microbial communities that efficiently breakdown plant materials in nature are species rich and secrete a myriad of enzymes to perform "community-level" metabolism of lignocellulose. Single-species approaches are, therefore, likely to miss important aspects of lignocellulose degradation that will be central to optimizing commercial processes. Results: Here, we investigated the microbial degradation of wheat straw in liquid cultures that had been inoculated with wheat straw compost. Samples taken at selected time points were subjected to multi-omics analysis with the aim of identifying new microbial mechanisms for lignocellulose degradation that could be applied in industrial pretreatment of feedstocks. Phylogenetic composition of the community, based on sequenced bacterial and eukaryotic ribosomal genes, showed a gradual decrease in complexity and diversity over time due to microbial enrichment. Taxonomic affiliation of bacterial species showed dominance of Bacteroidetes and Proteobacteria and high relative abundance of genera Asticcacaulis, Leadbetterella and Truepera. The eukaryotic members of the community were enriched in peritrich ciliates from genus Telotrochidium that thrived in the liquid cultures compared to fungal species that were present in low abundance. A targeted metasecretome approach combined with metatranscriptomics analysis, identified 1127 proteins and showed the presence of numerous carbohydrate-active enzymes extracted from the biomassbound fractions and from the culture supernatant. This revealed a wide array of hydrolytic cellulases, hemicellulases and carbohydrate-binding modules involved in lignocellulose degradation. The expression of these activities correlated to the changes in the biomass composition observed by FTIR and ssNMR measurements. Conclusions: A combination of mass spectrometry-based proteomics coupled with metatranscriptomics has enabled the identification of a large number of lignocellulose degrading enzymes that can now be further explored for the development of improved enzyme cocktails for the treatment of plant-based feedstocks. In addition to the expected carbohydrate-active enzymes, our studies reveal a large number of unknown proteins, some of which may play a crucial role in community-based lignocellulose degradation. MenosBackground: Lignocellulose is one of the most abundant forms of fixed carbon in the biosphere. Current industrial approaches to the degradation of lignocellulose employ enzyme mixtures, usually from a single fungal species, which are only effective in hydrolyzing polysaccharides following biomass pre-treatments. While the enzymatic mechanisms of lignocellulose degradation have been characterized in detail in individual microbial species, the microbial communities that efficiently breakdown plant materials in nature are species rich and secrete a myriad of enzymes to perform "community-level" metabolism of lignocellulose. Single-species approaches are, therefore, likely to miss important aspects of lignocellulose degradation that will be central to optimizing commercial processes. Results: Here, we investigated the microbial degradation of wheat straw in liquid cultures that had been inoculated with wheat straw compost. Samples taken at selected time points were subjected to multi-omics analysis with the aim of identifying new microbial mechanisms for lignocellulose degradation that could be applied in industrial pretreatment of feedstocks. Phylogenetic composition of the community, based on sequenced bacterial and eukaryotic ribosomal genes, showed a gradual decrease in complexity and diversity over time due to microbial enrichment. Taxonomic affiliation of bacterial species showed dominance of Bacteroidetes and Proteobacteria and high relative abundance of genera Asticcacau... Mostrar Tudo |
Palavras-Chave: |
CAZy; Metasecretome. |
Thesaurus NAL: |
Lignocellulose. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/186133/1/2018-044.pdf
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Marc: |
LEADER 03644naa a2200301 a 4500 001 2099501 005 2021-11-11 008 2018 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1186/s13068-018-1164-2$2DOI 100 1 $aALESSI, A. M. 245 $aDefining functional diversity for lignocellulose degradation in a microbial community using multi-omics studies.$h[electronic resource] 260 $c2018 520 $aBackground: Lignocellulose is one of the most abundant forms of fixed carbon in the biosphere. Current industrial approaches to the degradation of lignocellulose employ enzyme mixtures, usually from a single fungal species, which are only effective in hydrolyzing polysaccharides following biomass pre-treatments. While the enzymatic mechanisms of lignocellulose degradation have been characterized in detail in individual microbial species, the microbial communities that efficiently breakdown plant materials in nature are species rich and secrete a myriad of enzymes to perform "community-level" metabolism of lignocellulose. Single-species approaches are, therefore, likely to miss important aspects of lignocellulose degradation that will be central to optimizing commercial processes. Results: Here, we investigated the microbial degradation of wheat straw in liquid cultures that had been inoculated with wheat straw compost. Samples taken at selected time points were subjected to multi-omics analysis with the aim of identifying new microbial mechanisms for lignocellulose degradation that could be applied in industrial pretreatment of feedstocks. Phylogenetic composition of the community, based on sequenced bacterial and eukaryotic ribosomal genes, showed a gradual decrease in complexity and diversity over time due to microbial enrichment. Taxonomic affiliation of bacterial species showed dominance of Bacteroidetes and Proteobacteria and high relative abundance of genera Asticcacaulis, Leadbetterella and Truepera. The eukaryotic members of the community were enriched in peritrich ciliates from genus Telotrochidium that thrived in the liquid cultures compared to fungal species that were present in low abundance. A targeted metasecretome approach combined with metatranscriptomics analysis, identified 1127 proteins and showed the presence of numerous carbohydrate-active enzymes extracted from the biomassbound fractions and from the culture supernatant. This revealed a wide array of hydrolytic cellulases, hemicellulases and carbohydrate-binding modules involved in lignocellulose degradation. The expression of these activities correlated to the changes in the biomass composition observed by FTIR and ssNMR measurements. Conclusions: A combination of mass spectrometry-based proteomics coupled with metatranscriptomics has enabled the identification of a large number of lignocellulose degrading enzymes that can now be further explored for the development of improved enzyme cocktails for the treatment of plant-based feedstocks. In addition to the expected carbohydrate-active enzymes, our studies reveal a large number of unknown proteins, some of which may play a crucial role in community-based lignocellulose degradation. 650 $aLignocellulose 653 $aCAZy 653 $aMetasecretome 700 1 $aBIRD, S. M. 700 1 $aOATES, N. C. 700 1 $aLI, Y. 700 1 $aDOWLE, A. A. 700 1 $aNOVOTNY, E. H. 700 1 $aAZEVEDO, E. R. de 700 1 $aBENNETT, J. P. 700 1 $aPOLIKARPOV, I. 700 1 $aYOUNG, J. P. W. 700 1 $aMCQUEEN-MASON, S. J. 700 1 $aBRUCE, N. C. 773 $tBiotechnology for Biofuels$gv. 11, article 166, 2018.
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