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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
03/10/2022 |
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Data da última atualização: |
13/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MOREIRA, V. J. V; LOURENCO, I. T.; BASSO, M. F.; SÁ. M. E. L. de; MORGANTE, C. V.; MELO, B. P.; ARRAES, F. B. M.; SÁ, D. M. de; SILVA, M. C. M. da; ENGLER, J. de A.; SA, M. F. G. de. |
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Afiliação: |
VALDEIR JUNIO VAZ MOREIRA, UnB; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; MARCOS FERNANDO BASSO, National Institute of Science and Technology - INCT; MARIA EUGÊNIA LISEI DE SA, UnB; CAROLINA VIANNA MORGANTE, CPATSA; BRUNO PAES DE MELO, UFV; FABRÍCIO BARBOSA MONTEIRO ARRAES, National Institute of Science and Technology - INCT; DIOGO MARTINS DE SA, UnB; MARIA CRISTINA MATTAR DA SILVA, Cenargen; JANICE DE ALMEIDA ENGLER, National Institute of Science and Technology - INCT; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Minc03328 effector gene down regulation severely affects Meloidogyne incognita parasitism in transgenic Arabidopsis thaliana. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Planta, v. 255, n. 2, 2022. |
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Idioma: |
Inglês |
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Conteúdo: |
Minc03328 effector gene downregulation triggered by in planta RNAi strategy strongly reduced plant susceptibility to Meloidogyne incognita and suggests that Minc03328 gene is a promising target for the development of genetically engineered crops to improve plant tolerance to M. incognita. Meloidogyne incognita is the most economically important species of root-knot nematodes (RKN) and causes severe damage to crops worldwide. M. incognita secretes several effector proteins to suppress the host plant defense response, and manipulate the plant cell cycle and other plant processes facilitating its parasitism. Different secreted effector proteins have already been identified in M. incognita, but not all have been characterized or have had the confirmation of their involvement in nematode parasitism in their host plants. Herein, we characterized the Minc03328 (Minc3s00020g01299) effector gene, confirmed its higher expression in the early stages of M. incognita parasitism in plants, as well as the accumulation of the Minc03328 effector protein in subventral glands and its secretion. We also discuss the potential for simultaneous downregulation of its paralogue Minc3s00083g03984 gene. Using the in planta RNA interference strategy, Arabidopsis thaliana plants overexpressing double-stranded RNA (dsRNA) were generated to specifically targeting and downregulating the Minc03328 gene during nematode parasitism. Transgenic Minc03328-dsRNA lines that significantly downregulated Minc03328 gene expression during M. incognita parasitism were significantly less susceptible. The number of galls, egg masses, and [galls/egg masses] ratio were reduced in these transgenic lines by up to 85%, 90%, and 87%, respectively. Transgenic Minc03328-dsRNA lines showed the presence of fewer and smaller galls, indicating that parasitism was hindered. Overall, data herein strongly suggest that Minc03328 effector protein is important for M. incognita parasitism establishment. As well, the in planta Minc03328-dsRNA strategy demonstrated high biotechnological potential for developing crop species that could efficiently control RKN in the field. MenosMinc03328 effector gene downregulation triggered by in planta RNAi strategy strongly reduced plant susceptibility to Meloidogyne incognita and suggests that Minc03328 gene is a promising target for the development of genetically engineered crops to improve plant tolerance to M. incognita. Meloidogyne incognita is the most economically important species of root-knot nematodes (RKN) and causes severe damage to crops worldwide. M. incognita secretes several effector proteins to suppress the host plant defense response, and manipulate the plant cell cycle and other plant processes facilitating its parasitism. Different secreted effector proteins have already been identified in M. incognita, but not all have been characterized or have had the confirmation of their involvement in nematode parasitism in their host plants. Herein, we characterized the Minc03328 (Minc3s00020g01299) effector gene, confirmed its higher expression in the early stages of M. incognita parasitism in plants, as well as the accumulation of the Minc03328 effector protein in subventral glands and its secretion. We also discuss the potential for simultaneous downregulation of its paralogue Minc3s00083g03984 gene. Using the in planta RNA interference strategy, Arabidopsis thaliana plants overexpressing double-stranded RNA (dsRNA) were generated to specifically targeting and downregulating the Minc03328 gene during nematode parasitism. Transgenic Minc03328-dsRNA lines that significantly downregulated Minc03328 ge... Mostrar Tudo |
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Palavras-Chave: |
Interação planta-nematóide; Minc03328; Nematoide das galhas; Novas ferramentas biotecnológica; Planta RNAi; Proteção de cultivos; Proteína efetora. |
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Thesagro: |
Meloidogyne Incognita; Nematóide; Planta. |
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Thesaurus Nal: |
Plants (botany); Root-knot nematodes. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1147069/1/Minc03328-effector-gene-downregulation-2022.pdf
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Marc: |
LEADER 03276naa a2200385 a 4500
001 2147069
005 2023-01-13
008 2022 bl uuuu u00u1 u #d
100 1 $aMOREIRA, V. J. V
245 $aMinc03328 effector gene down regulation severely affects Meloidogyne incognita parasitism in transgenic Arabidopsis thaliana.$h[electronic resource]
260 $c2022
520 $aMinc03328 effector gene downregulation triggered by in planta RNAi strategy strongly reduced plant susceptibility to Meloidogyne incognita and suggests that Minc03328 gene is a promising target for the development of genetically engineered crops to improve plant tolerance to M. incognita. Meloidogyne incognita is the most economically important species of root-knot nematodes (RKN) and causes severe damage to crops worldwide. M. incognita secretes several effector proteins to suppress the host plant defense response, and manipulate the plant cell cycle and other plant processes facilitating its parasitism. Different secreted effector proteins have already been identified in M. incognita, but not all have been characterized or have had the confirmation of their involvement in nematode parasitism in their host plants. Herein, we characterized the Minc03328 (Minc3s00020g01299) effector gene, confirmed its higher expression in the early stages of M. incognita parasitism in plants, as well as the accumulation of the Minc03328 effector protein in subventral glands and its secretion. We also discuss the potential for simultaneous downregulation of its paralogue Minc3s00083g03984 gene. Using the in planta RNA interference strategy, Arabidopsis thaliana plants overexpressing double-stranded RNA (dsRNA) were generated to specifically targeting and downregulating the Minc03328 gene during nematode parasitism. Transgenic Minc03328-dsRNA lines that significantly downregulated Minc03328 gene expression during M. incognita parasitism were significantly less susceptible. The number of galls, egg masses, and [galls/egg masses] ratio were reduced in these transgenic lines by up to 85%, 90%, and 87%, respectively. Transgenic Minc03328-dsRNA lines showed the presence of fewer and smaller galls, indicating that parasitism was hindered. Overall, data herein strongly suggest that Minc03328 effector protein is important for M. incognita parasitism establishment. As well, the in planta Minc03328-dsRNA strategy demonstrated high biotechnological potential for developing crop species that could efficiently control RKN in the field.
650 $aPlants (botany)
650 $aRoot-knot nematodes
650 $aMeloidogyne Incognita
650 $aNematóide
650 $aPlanta
653 $aInteração planta-nematóide
653 $aMinc03328
653 $aNematoide das galhas
653 $aNovas ferramentas biotecnológica
653 $aPlanta RNAi
653 $aProteção de cultivos
653 $aProteína efetora
700 1 $aLOURENCO, I. T.
700 1 $aBASSO, M. F.
700 1 $aSÁ. M. E. L. de
700 1 $aMORGANTE, C. V.
700 1 $aMELO, B. P.
700 1 $aARRAES, F. B. M.
700 1 $aSÁ, D. M. de
700 1 $aSILVA, M. C. M. da
700 1 $aENGLER, J. de A.
700 1 $aSA, M. F. G. de
773 $tPlanta$gv. 255, n. 2, 2022.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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| Registros recuperados : 40 | |
| 4. |  | SA, M. E. L. de; SILVA, H. E. da; SILVA, C. S.; SANTOS, C. B. Indução de embriogênese somática em macaúba. In: CONGRESSO DA REDE BRASILEIRA DE TECNOLOGIA DE BIODIESEL, 3., 2009, Brasília, DF. Anais... Brasília, DF: MCT: MBC, 2009. p. 527-528.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
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| 5. | | SÁ, M. E. L. de; CARRÃO-PANIZZI, M. C.; ARANTES, N. E.; ZITO, R. K. Influência da época e da população de plantas no rendimento de soja hortaliça. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 30., 2008, Rio Verde. Resumos... Londrina: Embrapa Soja, 2008. p. 59-61. (Embrapa Soja. Documentos, 304). Organizado por Odilon Ferreira Saraiva, César de Castro, Regina Maria Villas Bôas de Campos Leite, Fábio Alvares de Oliveira.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 6. | | SÁ, M. E. L. de; ZITO, R. K.; MORAIS, F. C.; ARANTES, N. E. Fungicide effect in soybean sensorial characteristics in two environments. In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. p. 287. (Embrapa Soja. Documentos, 228). Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.| Biblioteca(s): Embrapa Soja. |
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| 10. | | SÁ, M. E. L. de; ARANTES, N. E.; MORAIS, F. C. Sensorial characterization of soybean genotypes. In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. p. 105. (Embrapa Soja. Documentos, 228). Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.| Biblioteca(s): Embrapa Soja. |
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| 13. | | FERREIRA, S. D.; SÁ, M. E. L. de; ARANTES, N. E; ZITO, R. K.; PAES, J. M. V. Dinâmica populacional e ciclo de vida do fitonematóide Meloidogyne javanica em raízes de soja. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 28., 2006, Uberaba. Resumos... Londrina: Embrapa Soja: Fundação Meridional: Fundação Triângulo, 2006. p. 175-177. (Embrapa Soja. Documentos, 272). Organizado por Odilon Ferreira Saraiva, Regina Maria Villas Boas de Campos Leite, Janete Lasso Ortiz.| Biblioteca(s): Embrapa Soja. |
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| 14. | | CARRÃO-PANIZZI, M. C.; OLIVEIRA, M. A.; MANDARINO, J. M. G. L.; SANTOS, H. P.; SÁ, M. E. L. de; OLIVEIRA, A. C. B. Avaliação da cultivar BRS 267 para consumo como hortaliça. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO SUL, 36., 2008, Porto Alegre. Ata e resumos. Porto Alegre: Fepagro, 2008. p. 30.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 15. | | PANIZZI, M. C. C.; OLIVEIRA, M. A. de; MANDARINO, J. M. G.; SANTOS, H. P. dos; SÁ, M. E. L. de; OLIVEIRA, A. C. B. Avaliação da cultivar BRS 267 para consumo como hortaliça. In: REUNIÃO DA PESQUISA DE SOJA DA REGIÃO SUL, 36. , 2008, Porto Alegre. Atas e resumos... Porto Alegre: Fepagro, 2008. p. 30.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| 16. | | SÁ, M. E. L. de; MONTEBIANCO, C. de B.; BERNARDINO, M. C.; BARRETO-BERGTER, E.; SILVA, P. L. R.; SA, M. F. G. de; VASLIN, M. F. S. Controle de Meloidogyne incognita em algodoeiro mediado por uma glicoproteína de Cladosporium herbarum. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 8, 2023, Florianópolis, SC. Anais... Florianópolis: SBG, 2023. p. 112| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 17. | | LOPES, M. J. C.; CAMPOS, M. de A.; PAIVA, L. V.; SÁ, M. E. L. de; ARANTES, N. E.; ZITO, R. K.; GROSSI-DE-SÁ, M. F. Changes in gene expression in resistant soybean genotype during interaction with Meloidogyne javanica. In: WORLD SOYBEAN RESEARCH CONFERENCE, 8., 2009, Beijing. Developing a global soy blueprint for a safe secure and sustainable supply: abstracts. Beijing: Chinese Academy of Agricultural Sciences: Institute of Crop Science, 2009. p. 142, ref. P363. WSRC 2009. Editado por Lijuan Qiu, Rongxia Guan, Jian Jin, Qijan Song, Shuntang Guo, Wenbin Li, Yuanchao Wang, Tianfu Han, Xiaobing Liu, Deyue Yu, Lianzhou Jiang, Deliang Peng.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 18. | | LEMES, S. V. da R.; CAVALCANTE, R. dos S.; SÁ, M. E. L. de; MORGANTE, C. V.; SA, M. F. G. de. Development of cotton plants with reduced susceptibility to Meloidogyne incognita through RNA interference approach. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 53., 2023, Brasília, DF. Anais... Brasília, DF: Sociedade Brasileira de Fitopatologia, 2023. p. 585.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| 19. | | SÁ, M. E. L. de; CARRÃO-PANIZZI, M. C.; ARANTES, N. E.; ZITO, R. K.; SILVA, T. R.; RESENDE, P. R. N.; PAES, J. M. V. Influência da poda e população de plantas no rendimento da soja hortaliça. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 28., 2006, Uberaba. Resumos... Londrina: Embrapa Soja: Fundação Meridional: Fundação Triângulo, 2006. p. 386-388. (Embrapa Soja. Documentos, 272). Organizado por Odilon Ferreira Saraiva, Regina Maria Villas Boas de Campos Leite, Janete Lasso Ortiz.| Biblioteca(s): Embrapa Soja. |
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| 20. | | SÁ, M. E. L. de; ARANTES, N. E.; ZITO, R. K.; CARRÃO-PANIZZI, M. C.; MANDARINO, J. M. G.; BENASSI, V. T. Influência do ambiente na composição centesimal de diferentes genótipos de soja. In: CONGRESSO BRASILEIRO DE SOJA, 4., 2006, Londrina. Resumos... Londrina: Embrapa Soja, 2006. p. 151. Organizado por Odilon Ferreira Saraiva, Simone Ery Grosskopf.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 40 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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