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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo; Embrapa Trigo. |
Data corrente: |
04/09/2014 |
Data da última atualização: |
01/02/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ZHOU, G.; PEREIRA, J. F.; DELHAIZE, E.; ZHOU, M.; MAGALHAES, J. V.; RYAN, P. R. |
Afiliação: |
UNIVERSITY OF TASMANIA; JORGE FERNANDO PEREIRA, CNPT; CSIRO PLANT INDUSTRY; UNIVERSITY OF TASMANIA; JURANDIR VIEIRA DE MAGALHAES, CNPMS; CSIRO PLANT INDUSTRY. |
Título: |
Enhancing the aluminium tolerance of barley by expressing the citrate transporter genes SbMATE and FRD3. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Journal of Experimental Botany, London, v. 65, n. 9, p. 2381-2390, 2014. |
DOI: |
10.1093/jxb/eru121 |
Idioma: |
Inglês |
Conteúdo: |
Malate and citrate efflux from root apices is a mechanism of Al3+ tolerance in many plant species. Citrate efflux is facilitated by members of the MATE (multidrug and toxic compound exudation) family localized to the plasma membrane of root cells. Barley (Hordeum vulgare) is among the most Al3+-sensitive cereal species but the small genotypic variation in tolerance that is present is correlated with citrate efflux via a MATE transporter named HvAACT1. This study used a biotechnological approach to increase the Al3+ tolerance of barley by transforming it with two MATE genes that encode citrate transporters: SbMATE is the major Al3+-tolerance gene from sorghum whereas FRD3 is involved with Fe nutrition in Arabidopsis. Independent transgenic and null T3 lines were generated for both transgenes. Lines expressing SbMATE showed Al3+-activated citrate efflux from root apices and greater tolerance to Al3+ toxicity than nulls in hydroponic and short-term soil trials. Transgenic lines expressing FRD3 exhibited similar phenotypes except citrate release from roots occurred constitutively. The Al3+ tolerance of these lines was compared with previously generated transgenic barley lines overexpressing the endogenous HvAACT1 gene and the TaALMT1 gene from wheat. Barley lines expressing TaALMT1 showed significantly greater Al3+ tolerance than all lines expressing MATE genes. This study highlights the relative efficacy of different organic anion transport proteins for increasing the Al3+ tolerance of an important crop species. MenosMalate and citrate efflux from root apices is a mechanism of Al3+ tolerance in many plant species. Citrate efflux is facilitated by members of the MATE (multidrug and toxic compound exudation) family localized to the plasma membrane of root cells. Barley (Hordeum vulgare) is among the most Al3+-sensitive cereal species but the small genotypic variation in tolerance that is present is correlated with citrate efflux via a MATE transporter named HvAACT1. This study used a biotechnological approach to increase the Al3+ tolerance of barley by transforming it with two MATE genes that encode citrate transporters: SbMATE is the major Al3+-tolerance gene from sorghum whereas FRD3 is involved with Fe nutrition in Arabidopsis. Independent transgenic and null T3 lines were generated for both transgenes. Lines expressing SbMATE showed Al3+-activated citrate efflux from root apices and greater tolerance to Al3+ toxicity than nulls in hydroponic and short-term soil trials. Transgenic lines expressing FRD3 exhibited similar phenotypes except citrate release from roots occurred constitutively. The Al3+ tolerance of these lines was compared with previously generated transgenic barley lines overexpressing the endogenous HvAACT1 gene and the TaALMT1 gene from wheat. Barley lines expressing TaALMT1 showed significantly greater Al3+ tolerance than all lines expressing MATE genes. This study highlights the relative efficacy of different organic anion transport proteins for increasing the Al3+ tole... Mostrar Tudo |
Palavras-Chave: |
Citrato; Tolerância ao alumínio; Transgênico. |
Thesagro: |
Cevada; Solo Ácido. |
Categoria do assunto: |
-- G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/117026/1/2014-Journal-of-Experimental-Botany-v65n9p2381.pdf
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Marc: |
LEADER 02283naa a2200253 a 4500 001 2007369 005 2015-02-01 008 2014 bl uuuu u00u1 u #d 024 7 $a10.1093/jxb/eru121$2DOI 100 1 $aZHOU, G. 245 $aEnhancing the aluminium tolerance of barley by expressing the citrate transporter genes SbMATE and FRD3.$h[electronic resource] 260 $c2014 520 $aMalate and citrate efflux from root apices is a mechanism of Al3+ tolerance in many plant species. Citrate efflux is facilitated by members of the MATE (multidrug and toxic compound exudation) family localized to the plasma membrane of root cells. Barley (Hordeum vulgare) is among the most Al3+-sensitive cereal species but the small genotypic variation in tolerance that is present is correlated with citrate efflux via a MATE transporter named HvAACT1. This study used a biotechnological approach to increase the Al3+ tolerance of barley by transforming it with two MATE genes that encode citrate transporters: SbMATE is the major Al3+-tolerance gene from sorghum whereas FRD3 is involved with Fe nutrition in Arabidopsis. Independent transgenic and null T3 lines were generated for both transgenes. Lines expressing SbMATE showed Al3+-activated citrate efflux from root apices and greater tolerance to Al3+ toxicity than nulls in hydroponic and short-term soil trials. Transgenic lines expressing FRD3 exhibited similar phenotypes except citrate release from roots occurred constitutively. The Al3+ tolerance of these lines was compared with previously generated transgenic barley lines overexpressing the endogenous HvAACT1 gene and the TaALMT1 gene from wheat. Barley lines expressing TaALMT1 showed significantly greater Al3+ tolerance than all lines expressing MATE genes. This study highlights the relative efficacy of different organic anion transport proteins for increasing the Al3+ tolerance of an important crop species. 650 $aCevada 650 $aSolo Ácido 653 $aCitrato 653 $aTolerância ao alumínio 653 $aTransgênico 700 1 $aPEREIRA, J. F. 700 1 $aDELHAIZE, E. 700 1 $aZHOU, M. 700 1 $aMAGALHAES, J. V. 700 1 $aRYAN, P. R. 773 $tJournal of Experimental Botany, London$gv. 65, n. 9, p. 2381-2390, 2014.
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Registro original: |
Embrapa Trigo (CNPT) |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
16/11/2022 |
Data da última atualização: |
16/11/2022 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
ARAÚJO, M. R. A. de; FARIAS, J. L. de S.; TABOSA, J. N.; CARVALHO, E. X. de. |
Afiliação: |
MARCELO RENATO ALVES DE ARAÚJO, CNPC; JORGE LUIS DE SALES FARIAS, CNPC; JOSÉ NILDO TABOSA; ERIC XAVIER DE CARVALHO. |
Título: |
Levantamento de variedades crioulas de milho no Semiárido do Ceará. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Revista RG News, v. 7, n. 2, p. 133, 2021. |
Idioma: |
Português |
Notas: |
Edição Anais do 5o. Simpósio da Rede de Recursos Genéticos Vegetais do Nordeste, Mossoró, RN, 10 a 12 novembro de 2021. |
Conteúdo: |
O déficit hídrico existente no semiárido brasileiro, sua escassez de recursos e mudanças climáticas se traduz como um dos desafios dos agricultores na produção de alimentos. O objetivo do presente trabalho foi analisar o manejo da agrobiodiversidade em variedades da agricultura tradicional de milho (Zea mays L.), a partir do conhecimento dos agricultores da região do semiárido cearense. O estudo foi conduzido a partir de levantamento de campo, junto a 190 agricultores, e realizado em 18 comunidades distribuídas em nove municípios do semiárido cearense, no ano de 2016. |
Palavras-Chave: |
Manejo da agrobiodiversidade; Resgate de germoplasma. |
Thesagro: |
Agricultura Familiar; Zea Mays. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148292/1/cnpc-2021-Art106.pdf
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Marc: |
LEADER 01317nam a2200205 a 4500 001 2148292 005 2022-11-16 008 2021 bl uuuu u00u1 u #d 100 1 $aARAÚJO, M. R. A. de 245 $aLevantamento de variedades crioulas de milho no Semiárido do Ceará.$h[electronic resource] 260 $aRevista RG News, v. 7, n. 2, p. 133$c2021 500 $aEdição Anais do 5o. Simpósio da Rede de Recursos Genéticos Vegetais do Nordeste, Mossoró, RN, 10 a 12 novembro de 2021. 520 $aO déficit hídrico existente no semiárido brasileiro, sua escassez de recursos e mudanças climáticas se traduz como um dos desafios dos agricultores na produção de alimentos. O objetivo do presente trabalho foi analisar o manejo da agrobiodiversidade em variedades da agricultura tradicional de milho (Zea mays L.), a partir do conhecimento dos agricultores da região do semiárido cearense. O estudo foi conduzido a partir de levantamento de campo, junto a 190 agricultores, e realizado em 18 comunidades distribuídas em nove municípios do semiárido cearense, no ano de 2016. 650 $aAgricultura Familiar 650 $aZea Mays 653 $aManejo da agrobiodiversidade 653 $aResgate de germoplasma 700 1 $aFARIAS, J. L. de S. 700 1 $aTABOSA, J. N. 700 1 $aCARVALHO, E. X. de
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Embrapa Caprinos e Ovinos (CNPC) |
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